Restriction map for
pFN21AE0892
(Circular) (Six-base) MAPSORT of: pFN21AE0892.seq Check: 3698 from: 1 to: 5677
pFN21AE0892 5677 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5677
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 3740 5124 5392 1632
Size: 456 1652 1384 268 1917
Fragments arranged by size:
1917 1652 1384 456 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 2479 4576 5553 1203
Size: 1276 2097 977 1327
Fragments arranged by size:
2097 1327 1276 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 2744 1166
Size: 1578 4099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 2424 2699 3420 4992 2424
Size: 275 721 1572 3109
Fragments arranged by size:
3109 1572 721 275
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2036 2664 2986 3212 3364 5473 1493
Size: 543 628 322 226 152 2109 1697
Fragments arranged by size:
2109 1697 628 543 322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 3002 1870
Size: 73 1059 4545
Fragments arranged by size:
4545 1059 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2998 4453 618
Size: 325 217 627 1211 1455 1842
Fragments arranged by size:
1842 1455 1211 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2996 1391
Size: 1605 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2076 928
Size: 971 177 4529
Fragments arranged by size:
4529 971 177
BbvCI CC'TCA_GC
Cuts at: 2692 2692
Size: 5677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 3738 4894 1790
Size: 1948 1156 2573
Fragments arranged by size:
2573 1948 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3408 4366 442
Size: 416 53 83 399 24 1991 958 1753
Fragments arranged by size:
1991 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4337 1239
Size: 3098 2579
Bpu10I CC'TnA_GC
Cuts at: 2192 2692 2192
Size: 500 5177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2666 3802 4667 4965 2666
Size: 1136 865 298 3378
Fragments arranged by size:
3378 1136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 2340 5671 2340
Size: 3331 2346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3484 3866 4501 275
Size: 53 83 186 936 1951 382 635 1451
Fragments arranged by size:
1951 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2469 2499 1178
Size: 30 165 30 1066 30 4356
Fragments arranged by size:
4356 1066 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 2563 1186
Size: 861 516 4300
Fragments arranged by size:
4300 861 516
BseYI C'CCAG_C
Cuts at: 4880 5376 4880
Size: 496 5181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 2441 3188 3281 2441
Size: 747 93 4837
Fragments arranged by size:
4837 747 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2996 3738 3823 4894 1391
Size: 399 1206 742 85 1071 2174
Fragments arranged by size:
2174 1206 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 3032 844
Size: 1888 300 3489
Fragments arranged by size:
3489 1888 300
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 2510 96
Size: 2414 3263
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 2249 3737 4749 2249
Size: 1488 1012 3177
Fragments arranged by size:
3177 1488 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4186
Fragments arranged by size:
4186 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2656 487
Size: 683 56 1430 3508
Fragments arranged by size:
3508 1430 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 3987 4007 3193
Size: 794 20 4863
Fragments arranged by size:
4863 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2598 3124
Size: 54 1425 50 275 18 528 240 526
Cuts at: 3124 4013 5642 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 528 526 275 240 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3607 1353
Size: 2254 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 3740 4337 5124 5392 1239
Size: 393 456 1652 597 787 268 1524
Fragments arranged by size:
1652 1524 787 597 456 393 268
EcoRI G'AATT_C
Cuts at: 2664 2986 3364 2664
Size: 322 378 4977
Fragments arranged by size:
4977 378 322
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 4824 1678
Size: 3146 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 2914 2946 4318
Size: 32 135 32 541 32 769 32 1372
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1372 769 541 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 2844 3021 3267 669
Size: 988 281 610 296 177 246 3079
Fragments arranged by size:
3079 988 610 296 281 246 177
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 4791 4975 1305
Size: 735 2751 184 2007
Fragments arranged by size:
2751 2007 735 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 2600 10
Size: 54 1425 343 528 240 3087
Fragments arranged by size:
3087 1425 528 343 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2120 2408 2744 3635 3994 4153
Size: 648 843 111 288 336 891 359 159
Cuts at: 4153 518
Size: 2042
Fragments arranged by size:
2042 891 843 648 359 336 288 159
111
MspA1I CmG'CkG
Cuts at: 1606 2370 2699 3093 3770 4918 5163 1606
Size: 764 329 394 677 1148 245 2120
Fragments arranged by size:
2120 1148 764 677 394 329 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4186
Fragments arranged by size:
4186 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2483 3035 4580 1132
Size: 97 1254 552 1545 2229
Fragments arranged by size:
2229 1545 1254 552 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 2479 4576 2479
Size: 2097 3580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PstI C_TGCA'G
Cuts at: 830 2729 3029 830
Size: 1899 300 3478
Fragments arranged by size:
3478 1899 300
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2699 2699
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5677
SfcI C'TryA_G
Cuts at: 826 1045 2725 2869 3025 4160 4841 5032 826
Size: 219 1680 144 156 1135 681 191 1471
Fragments arranged by size:
1680 1471 1135 681 219 191 156 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2681 3781 4682 4944 820
Size: 197 926 738 1100 901 262 1553
Fragments arranged by size:
1553 1100 926 901 738 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2004 3116 513
Size: 552 939 1112 3074
Fragments arranged by size:
3074 1112 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2052 2829 3704 3721 3874 4059 4504
Size: 1255 472 777 875 17 153 185 445
Cuts at: 4504 325
Size: 1498
Fragments arranged by size:
1498 1255 875 777 472 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2510 3923 5635
Size: 274 80 33 51 1443 533 1413 1712
Cuts at: 5635 96
Size: 138
Fragments arranged by size:
1712 1443 1413 533 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 3806 2040
Size: 1766 3911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI