Restriction map for
pFN21AE0891
(Circular) (Six-base) MAPSORT of: pFN21AE0891.seq Check: 1828 from: 1 to: 5653
pFN21AE0891 5653 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2781 2996 1937
Size: 844 215 4594
Fragments arranged by size:
4594 844 215
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2010 3716 5100 5368 1632
Size: 378 1706 1384 268 1917
Fragments arranged by size:
1917 1706 1384 378 268
AflII C'TTAA_G
Cuts at: 820 1017 2560 820
Size: 197 1543 3913
Fragments arranged by size:
3913 1543 197
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 2064 4382 2064
Size: 2318 3335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2702 2883 3396 4968 2702
Size: 181 513 1572 3387
Fragments arranged by size:
3387 1572 513 181
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2418 2978 1870
Size: 73 475 560 4545
Fragments arranged by size:
4545 560 475 73
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2388 2615 2974 4429 618
Size: 325 217 627 601 227 359 1455 1842
Fragments arranged by size:
1842 1455 627 601 359 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2917 2972 1391
Size: 1526 55 4072
Fragments arranged by size:
4072 1526 55
BbeI G_GCGC'C
Cuts at: 2619 2619
Size: 5653
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2016 2339 928
Size: 971 117 323 4242
Fragments arranged by size:
4242 971 323 117
BbvCI CC'TCA_GC
Cuts at: 2695 2695
Size: 5653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2156 4264
Size: 107 122 71 631 605 337 147 2108
Cuts at: 4264 136
Size: 1525
Fragments arranged by size:
2108 1525 631 605 337 147 122 107
71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2244 2532 2908 3714 4870 1790
Size: 454 288 376 806 1156 2573
Fragments arranged by size:
2573 1156 806 454 376 288
BmgBI CAC'GTC
Cuts at: 1318 2467 1318
Size: 1149 4504
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2936 2053
Size: 883 4770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 4313 1239
Size: 1430 1644 2579
Fragments arranged by size:
2579 1644 1430
Bpu10I CC'TnA_GC
Cuts at: 2695 2695
Size: 5653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2377 3444 493
Size: 711 1173 1067 2702
Fragments arranged by size:
2702 1173 1067 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 3460 3842 4477
Size: 53 83 186 936 1083 844 382 635
Cuts at: 4477 275
Size: 1451
Fragments arranged by size:
1451 1083 936 844 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2456 2486 1178
Size: 30 165 30 1053 30 4345
Fragments arranged by size:
4345 1053 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5653
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2576 2685 3714 3799 4870 2576
Size: 109 1029 85 1071 3359
Fragments arranged by size:
3359 1071 1029 109 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2532 2576 2685 2908 2917 2972
Size: 399 454 288 44 109 223 9 55
Cuts at: 2972 3714 3799 4870 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 288 223 109
85 55 44 9
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2772 4148 4322 65
Size: 1183 1524 1376 174 1396
Fragments arranged by size:
1524 1396 1376 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 2838 2896 96
Size: 2742 58 2853
Fragments arranged by size:
2853 2742 58
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 2407 2569 3713 4725 2407
Size: 162 1144 1012 3335
Fragments arranged by size:
3335 1144 1012 162
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 2125 2251 2263 2287 2125
Size: 126 12 24 5491
Fragments arranged by size:
5491 126 24 12
BstYI r'GATC_y
Cuts at: 1972 2934 2983 3350 3735 3752 5204 5290 5302
Size: 962 49 367 385 17 1452 86 12
Cuts at: 5302 5648 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 962 385 367 346 86 49
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2358 513
Size: 552 939 354 3808
Fragments arranged by size:
3808 939 552 354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2653 2907 487
Size: 683 56 1427 254 3233
Fragments arranged by size:
3233 1427 683 254 56
BtsI GCAGTG_nn'
Cuts at: 2628 3169 3963 3983 2628
Size: 541 794 20 4298
Fragments arranged by size:
4298 794 541 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DraIII CAC_nnn'GTG
Cuts at: 2527 2527
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2253 2355
Size: 54 1425 50 275 18 285 138 102
Cuts at: 2355 2379 2826 3100 3989 5618 8
Size: 24 447 274 889 1629 43
Fragments arranged by size:
1629 1425 889 447 285 275 274 138
102 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2762 3583 1353
Size: 1409 821 3423
Fragments arranged by size:
3423 1409 821
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2010 2669 3716 4313 5100 5368 1239
Size: 393 378 659 1047 597 787 268 1524
Fragments arranged by size:
1524 1047 787 659 597 393 378 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2704 2704
Size: 5653
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 2431 4159 2431
Size: 1728 3925
HaeII r_GCGC'y
Cuts at: 1678 2619 4800 1678
Size: 941 2181 2531
Fragments arranged by size:
2531 2181 941
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2050 2082 4294 4326 4368
Size: 32 135 32 478 32 2212 32 42
Cuts at: 4368 4400 1373
Size: 32 2626
Fragments arranged by size:
2626 2212 478 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KasI G'GCGC_C
Cuts at: 2615 2615
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2255 2381 2828 10
Size: 54 1425 343 285 138 126 447 2835
Fragments arranged by size:
2835 1425 447 343 285 138 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 2834 2855 3611 3970 4129 518
Size: 648 1245 423 21 756 359 159 2042
Fragments arranged by size:
2042 1245 756 648 423 359 159 21
MspA1I CmG'CkG
Cuts at: 1606 2080 2596 2675 2689 3069 3746 4894 5139
Size: 474 516 79 14 380 677 1148 245
Cuts at: 5139 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 516 474 380 245 79
14
NarI GG'CG_CC
Cuts at: 2616 2616
Size: 5653
NcoI C'CATG_G
Cuts at: 513 1065 2004 2358 513
Size: 552 939 354 3808
Fragments arranged by size:
3808 939 552 354
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NruI TCG'CGA
Cuts at: 2892 2892
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2326 1348
Size: 978 4675
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PfoI T'CCnGG_A
Cuts at: 2068 2068
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2596 2675 2689 2596
Size: 79 14 5560
Fragments arranged by size:
5560 79 14
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2844 2875 3001 4136 4817 5008 826
Size: 219 1799 31 126 1135 681 191 1471
Fragments arranged by size:
1799 1471 1135 681 219 191 126 31
SfoI GGC'GCC
Cuts at: 2617 2617
Size: 5653
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2420 2980 1872
Size: 548 560 4545
Fragments arranged by size:
4545 560 548
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2560 3757 4658 4920 820
Size: 197 926 617 1197 901 262 1553
Fragments arranged by size:
1553 1197 926 901 617 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 3092 513
Size: 552 939 354 734 3074
Fragments arranged by size:
3074 939 734 552 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 2597 3680 3697 3850 4035 4480
Size: 1255 614 403 1083 17 153 185 445
Cuts at: 4480 325
Size: 1498
Fragments arranged by size:
1498 1255 1083 614 445 403 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2310 2838
Size: 274 80 33 51 1443 207 126 528
Cuts at: 2838 2896 3899 5611 96
Size: 58 1003 1712 138
Fragments arranged by size:
1712 1443 1003 528 274 207 138 126
80 58 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 2456 2488 5613 5645 2127
Size: 32 297 32 3125 32 2135
Fragments arranged by size:
3125 2135 297 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2418 2978 1870
Size: 548 560 4545
Fragments arranged by size:
4545 560 548
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI