Restriction map for
pFN21AE0887
(Circular) (Six-base) MAPSORT of: pFN21AE0887.seq Check: 8539 from: 1 to: 5758
pFN21AE0887 5758 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2846 2846
Size: 5758
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2410 3101 1937
Size: 473 691 4594
Fragments arranged by size:
4594 691 473
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 2778 3885 4258 1563
Size: 1215 1107 373 3063
Fragments arranged by size:
3063 1215 1107 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2816 3821 5205 5473 1632
Size: 1184 1005 1384 268 1917
Fragments arranged by size:
1917 1384 1184 1005 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2347 1184
Size: 1163 4595
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2600 2632 2600
Size: 32 5726
AlwNI CAG_nnn'CTG
Cuts at: 3501 5073 3501
Size: 1572 4186
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 3045 3067 3293 3445 5554 1493
Size: 1552 22 226 152 2109 1697
Fragments arranged by size:
2109 1697 1552 226 152 22
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 3083 1870
Size: 73 1140 4545
Fragments arranged by size:
4545 1140 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3079 4534 618
Size: 325 217 627 1292 1455 1842
Fragments arranged by size:
1842 1455 1292 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2531 2538 3077 1391
Size: 1140 7 539 4072
Fragments arranged by size:
4072 1140 539 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3006 928
Size: 971 1107 3680
Fragments arranged by size:
3680 1107 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2530 2564 3949 3983 1903
Size: 34 593 34 1385 34 3678
Fragments arranged by size:
3678 1385 593 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 2520 1548
Size: 972 4786
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 3819 4975 1790
Size: 2029 1156 2573
Fragments arranged by size:
2573 2029 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2733 3489 4447
Size: 416 53 83 399 24 1316 756 958
Cuts at: 4447 442
Size: 1753
Fragments arranged by size:
1753 1316 958 756 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2468 4418 1239
Size: 962 267 1950 2579
Fragments arranged by size:
2579 1950 962 267
Bpu10I CC'TnA_GC
Cuts at: 2811 2811
Size: 5758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2735 2866 4421 882
Size: 617 1236 131 1555 2219
Fragments arranged by size:
2219 1555 1236 617 131
BsaAI yAC'GTr
Cuts at: 493 1204 2488 3549 493
Size: 711 1284 1061 2702
Fragments arranged by size:
2702 1284 1061 711
BsaBI GATnn'nnATC
Cuts at: 2717 5752 2717
Size: 3035 2723
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2237 1186
Size: 1012 39 4707
Fragments arranged by size:
4707 1012 39
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2668 1163
Size: 573 932 4253
Fragments arranged by size:
4253 932 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3819 3904 4975 3819
Size: 85 1071 4602
Fragments arranged by size:
4602 1071 85
BsmI GAATG_Cn'
Cuts at: 2453 2578 3269 3362 2453
Size: 125 691 93 4849
Fragments arranged by size:
4849 691 125 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2531 2538 3077 3819 3904 4975 1391
Size: 399 741 7 539 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 539 399 85 7
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 2523 2568 3647 5377 2523
Size: 45 1079 1730 2904
Fragments arranged by size:
2904 1730 1079 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2846 3113 844
Size: 2002 267 3489
Fragments arranged by size:
3489 2002 267
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4253 4427 65
Size: 1183 3005 174 1396
Fragments arranged by size:
3005 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 2949 96
Size: 2853 2905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 2549 3818 4830 2549
Size: 1269 1012 3477
Fragments arranged by size:
3477 1269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstXI CCAn_nnnn'nTGG
Cuts at: 2707 2707
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2376 2637 2799 3088 3455 3840 3857 5309
Size: 404 261 162 289 367 385 17 1452
Cuts at: 5309 5395 5407 5753 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 404 385 367 346 289 261
162 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2856 513
Size: 552 1791 3415
Fragments arranged by size:
3415 1791 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 3274 4068 4088 3274
Size: 794 20 4944
Fragments arranged by size:
4944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 3205 4094
Size: 54 1425 50 275 18 660 715 889
Cuts at: 4094 5723 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 715 660 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2873 3688 1353
Size: 1520 815 3423
Fragments arranged by size:
3423 1520 815
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2468 2816 3821 4418 5205 5473
Size: 393 569 267 348 1005 597 787 268
Cuts at: 5473 1239
Size: 1524
Fragments arranged by size:
1524 1005 787 597 569 393 348 268
267
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 5758
FspI TGC'GCA
Cuts at: 2626 4264 2626
Size: 1638 4120
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2368 2400 4399
Size: 32 135 32 427 32 337 32 1999
Cuts at: 4399 4431 4473 4505 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1999 427 337 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2035 4872 5056 1305
Size: 730 2837 184 2007
Fragments arranged by size:
2837 2007 730 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 10
Size: 54 1425 343 660 3276
Fragments arranged by size:
3276 1425 660 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2747 3716 4075 4234 518
Size: 648 1581 969 359 159 2042
Fragments arranged by size:
2042 1581 969 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2179 3174 3851 4999 5244 1606
Size: 573 995 677 1148 245 2120
Fragments arranged by size:
2120 1148 995 677 573 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5206
NdeI CA'TA_TG
Cuts at: 387 2495 387
Size: 2108 3650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2531 1348
Size: 1183 4575
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PfoI T'CCnGG_A
Cuts at: 2561 2561
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2768 2800 3827 3859 5340 5372 2768
Size: 32 1027 32 1481 32 3154
Fragments arranged by size:
3154 1481 1027 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2843 3110 830
Size: 2013 267 3478
Fragments arranged by size:
3478 2013 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2179 2179
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2110 2414 2839 2986 3106 4241 4922
Size: 219 1065 304 425 147 120 1135 681
Cuts at: 4922 5113 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1065 681 425 304 219 191
147 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3862 4763 5025 820
Size: 197 926 1919 901 262 1553
Fragments arranged by size:
1919 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 2042 3682 5
Size: 47 1990 1640 2081
Fragments arranged by size:
2081 1990 1640 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 3197 513
Size: 552 2132 3074
Fragments arranged by size:
3074 2132 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 2517 3785 3802 3955 4140 4585
Size: 1255 522 415 1268 17 153 185 445
Cuts at: 4585 325
Size: 1498
Fragments arranged by size:
1498 1268 1255 522 445 415 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 2295 2949
Size: 274 80 33 51 1443 243 75 654
Cuts at: 2949 4004 5716 96
Size: 1055 1712 138
Fragments arranged by size:
1712 1443 1055 654 274 243 138 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 2780 3887 2780
Size: 1107 4651
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI