Restriction map for
pFN21AE0868
(Circular) (Six-base) MAPSORT of: pFN21AE0868.seq Check: 5321 from: 1 to: 5620
pFN21AE0868 5620 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2705 2705
Size: 5620
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3683 5067 5335 1632
Size: 2051 1384 268 1917
Fragments arranged by size:
2051 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 2201 2717 1166
Size: 1035 516 4069
Fragments arranged by size:
4069 1035 516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2891 2923 2000
Size: 32 859 32 4697
Fragments arranged by size:
4697 859 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2901 3363 4935 2901
Size: 462 1572 3586
Fragments arranged by size:
3586 1572 462
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2009 2929 3155 3307 5416 1493
Size: 516 920 226 152 2109 1697
Fragments arranged by size:
2109 1697 920 516 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2209 2941 4396 618
Size: 325 217 627 422 732 1455 1842
Fragments arranged by size:
1842 1455 732 627 422 325 217
BanII G_rGCy'C
Cuts at: 1391 2939 1391
Size: 1548 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2252 928
Size: 971 353 4296
Fragments arranged by size:
4296 971 353
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 2088 1548
Size: 540 5080
BfrBI ATG'CAT
Cuts at: 2533 2533
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2762 3681 4837 1790
Size: 424 548 919 1156 2573
Fragments arranged by size:
2573 1156 919 548 424
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2106 2306 3351
Size: 416 53 83 399 24 689 200 1045
Cuts at: 3351 4309 442
Size: 958 1753
Fragments arranged by size:
1753 1045 958 689 416 399 200 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2752 4280 1239
Size: 1513 1528 2579
Fragments arranged by size:
2579 1528 1513
Bpu10I CC'TnA_GC
Cuts at: 2490 2490
Size: 5620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 3745 4610 4908 2639
Size: 1106 865 298 3351
Fragments arranged by size:
3351 1106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 1186
Size: 1183 4437
BseYI C'CCAG_C
Cuts at: 2762 4823 5319 2762
Size: 2061 496 3063
Fragments arranged by size:
3063 2061 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2505 2548 3681 3766 4837 2505
Size: 43 1133 85 1071 3288
Fragments arranged by size:
3288 1133 1071 85 43
BsmI GAATG_Cn'
Cuts at: 3131 3224 3131
Size: 93 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2505 2548 2762 2939 3681 3766
Size: 399 424 291 43 214 177 742 85
Cuts at: 3766 4837 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 291 214 177
85 43
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 2382 2560 3509 5239 2382
Size: 178 949 1730 2763
Fragments arranged by size:
2763 1730 949 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2705 2975 844
Size: 1861 270 3489
Fragments arranged by size:
3489 1861 270
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2289 2595 4115 4289 65
Size: 1183 1041 306 1520 174 1396
Fragments arranged by size:
1520 1396 1183 1041 306 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 2808 96
Size: 2712 2908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2572 794
Size: 1778 3842
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 2278
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2658 2950 3317 3702 3719 5171 5257 5269
Size: 686 292 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 686 385 367 346 292 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2852 2852
Size: 5620
BtgI C'CryG_G
Cuts at: 513 1065 2004 2328 513
Size: 552 939 324 3805
Fragments arranged by size:
3805 939 552 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 2196 2208 2308 2825 3136 3930 3950 2196
Size: 12 100 517 311 794 20 3866
Fragments arranged by size:
3866 794 517 311 100 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2349 2674
Size: 54 1425 50 275 18 393 126 325
Cuts at: 2674 3067 3956 5585 8
Size: 393 889 1629 43
Fragments arranged by size:
1629 1425 889 393 393 325 275 126
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2707 3550 1353
Size: 1354 843 3423
Fragments arranged by size:
3423 1354 843
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2752 3683 4280 5067 5335 1239
Size: 393 1120 931 597 787 268 1524
Fragments arranged by size:
1524 1120 931 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2239 2271 2377 2409 4261
Size: 32 135 32 667 32 106 32 1852
Cuts at: 4261 4293 4335 4367 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1852 667 135 106 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2225 2351 10
Size: 54 1425 343 393 126 3279
Fragments arranged by size:
3279 1425 393 343 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 2150 2201 2276 2559 2600 2717
Size: 648 948 36 51 75 283 41 117
Cuts at: 2717 3578 3937 4096 518
Size: 861 359 159 2042
Fragments arranged by size:
2042 948 861 648 359 283 159 117
75 51 41 36
MspA1I CmG'CkG
Cuts at: 1606 3036 3713 4861 5106 1606
Size: 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2328 513
Size: 552 939 324 3805
Fragments arranged by size:
3805 939 552 324
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NsiI A_TGCA'T
Cuts at: 2535 2535
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2537 2978 4523 1132
Size: 97 1308 441 1545 2229
Fragments arranged by size:
2229 1545 1308 441 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2221 1108
Size: 1113 4507
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2871 2903 2871
Size: 32 5588
PstI C_TGCA'G
Cuts at: 830 2203 2730 2972 830
Size: 1373 527 242 3478
Fragments arranged by size:
3478 1373 527 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SapI GCTCTTCn'nnn_
Cuts at: 2707 2707
Size: 5620
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2199 2234 2726 2968 4103 4784 4975
Size: 219 1154 35 492 242 1135 681 191
Cuts at: 4975 826
Size: 1471
Fragments arranged by size:
1471 1154 1135 681 492 242 219 191
35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 3724 4625 4887 820
Size: 197 926 711 1070 901 262 1553
Fragments arranged by size:
1553 1070 926 901 711 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2004 2062 2328 3059 513
Size: 552 939 58 266 731 3074
Fragments arranged by size:
3074 939 731 552 266 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2288 3647 3664 3817 4002 4447 325
Size: 1255 708 1359 17 153 185 445 1498
Fragments arranged by size:
1498 1359 1255 708 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2154 2808 3866
Size: 274 80 33 51 1443 177 654 1058
Cuts at: 3866 5578 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1058 654 274 177 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2669 2257
Size: 412 5208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI