Restriction map for
pFN21AE0865
(Circular) (Six-base) MAPSORT of: pFN21AE0865.seq Check: 1408 from: 1 to: 5710
pFN21AE0865 5710 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2470 2792 2470
Size: 322 5388
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2762 3773 5157 5425 1632
Size: 1130 1011 1384 268 1917
Fragments arranged by size:
1917 1384 1130 1011 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 2799 1166
Size: 1633 4077
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 3453 5025 3453
Size: 1572 4138
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2013 2096 2130 2545 3019 3245 3397 5506
Size: 520 83 34 415 474 226 152 2109
Cuts at: 5506 1493
Size: 1697
Fragments arranged by size:
2109 1697 520 474 415 226 152 83
34
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3031 4486 618
Size: 325 217 627 1244 1455 1842
Fragments arranged by size:
1842 1455 1244 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3029 1391
Size: 1638 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BbvCI CC'TCA_GC
Cuts at: 2752 2757 2752
Size: 5 5705
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3901 3935 1903
Size: 34 50 34 1880 34 3678
Fragments arranged by size:
3678 1880 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2064 5705 2064
Size: 3641 2069
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 3771 4927 1790
Size: 1981 1156 2573
Fragments arranged by size:
2573 1981 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4370 1239
Size: 3131 2579
Bpu10I CC'TnA_GC
Cuts at: 2752 2757 2752
Size: 5 5705
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2267 4373 882
Size: 617 768 2106 2219
Fragments arranged by size:
2219 2106 768 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 2309 5704 2309
Size: 3395 2315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2664 2694 1178
Size: 30 165 30 1261 30 4194
Fragments arranged by size:
4194 1261 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5710
BseYI C'CCAG_C
Cuts at: 2107 4913 5409 2107
Size: 2806 496 2408
Fragments arranged by size:
2806 2408 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2359 3771 3856 4927 2359
Size: 1412 85 1071 3142
Fragments arranged by size:
3142 1412 1071 85
BsmI GAATG_Cn'
Cuts at: 2256 2724 3221 3314 2256
Size: 468 497 93 4652
Fragments arranged by size:
4652 497 468 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2359 3029 3771 3856 4927 1391
Size: 399 569 670 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 670 569 399 85
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2470 2792 3065 844
Size: 1626 322 273 3489
Fragments arranged by size:
3489 1626 322 273
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2194 4205 4379 65
Size: 1183 946 2011 174 1396
Fragments arranged by size:
2011 1396 1183 946 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 2089 1184
Size: 905 4805
BstYI r'GATC_y
Cuts at: 1972 2064 3040 3407 3792 3809 5261 5347 5359
Size: 92 976 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 976 385 367 346 92 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2843 2843
Size: 5710
BtgI C'CryG_G
Cuts at: 513 1065 2085 2925 513
Size: 552 1020 840 3298
Fragments arranged by size:
3298 1020 840 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 3157 4046
Size: 54 1425 50 275 18 498 829 889
Cuts at: 4046 5675 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 829 498 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 3640 1353
Size: 1011 1276 3423
Fragments arranged by size:
3423 1276 1011
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2762 3773 4370 5157 5425 1239
Size: 393 1130 1011 597 787 268 1524
Fragments arranged by size:
1524 1130 1011 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2096 3019 3397 2096
Size: 923 378 4409
Fragments arranged by size:
4409 923 378
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2577 4857 1678
Size: 899 2280 2531
Fragments arranged by size:
2531 2280 899
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2893 2925 4351 4383 4425
Size: 32 135 32 1321 32 1426 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 1426 1321 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2647 3054 3300 669
Size: 988 281 709 407 246 3079
Fragments arranged by size:
3079 988 709 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 2647 3300 2647
Size: 653 5057
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 2511 3309 2511
Size: 798 4912
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2892 2980 4824 5008 1305
Size: 1587 88 1844 184 2007
Fragments arranged by size:
2007 1844 1587 184 88
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 10
Size: 54 1425 343 498 3390
Fragments arranged by size:
3390 1425 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2468 2799 3668 4027 4186 518
Size: 648 1071 231 331 869 359 159 2042
Fragments arranged by size:
2042 1071 869 648 359 331 231 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2501 2756 3126 3803 4951 5196 1606
Size: 531 364 255 370 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 370 364 255 245
NcoI C'CATG_G
Cuts at: 513 1065 2085 2925 513
Size: 552 1020 840 3298
Fragments arranged by size:
3298 1020 840 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2673 2705 2673
Size: 32 5678
PstI C_TGCA'G
Cuts at: 830 3062 830
Size: 2232 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2137 2501 2756 2137
Size: 364 255 5091
Fragments arranged by size:
5091 364 255
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SapI GCTCTTCn'nnn_
Cuts at: 2364 2364
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2850 3058 4193 4874 5065 826
Size: 219 1805 208 1135 681 191 1471
Fragments arranged by size:
1805 1471 1135 681 219 208 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3814 4715 4977 820
Size: 197 926 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 2848 1517
Size: 1331 4379
StyI C'CwwG_G
Cuts at: 513 1065 2085 2122 2251 2377 2925 3149 513
Size: 552 1020 37 129 126 548 224 3074
Fragments arranged by size:
3074 1020 552 548 224 129 126 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2399 3737 3754 3907 4092 4537 325
Size: 1255 819 1338 17 153 185 445 1498
Fragments arranged by size:
1498 1338 1255 819 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 2680 3956
Size: 274 80 33 51 1443 264 439 1276
Cuts at: 3956 5668 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1276 439 274 264 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI