Restriction map for
pFN21AE0863
(Circular) (Six-base) MAPSORT of: pFN21AE0863.seq Check: 8153 from: 1 to: 5644
pFN21AE0863 5644 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2735 2735
Size: 5644
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 2987 1937
Size: 169 881 4594
Fragments arranged by size:
4594 881 169
Acc65I G'GTAC_C
Cuts at: 2444 2965 2444
Size: 521 5123
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3707 5091 5359 1632
Size: 2075 1384 268 1917
Fragments arranged by size:
2075 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaI G_GGCC'C
Cuts at: 2081 2908 2081
Size: 827 4817
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2036 2953 3179 3331 5440 1493
Size: 543 917 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2444 2675 2965 4420 618
Size: 325 217 627 657 231 290 1455 1842
Fragments arranged by size:
1842 1455 657 627 325 290 231 217
BanII G_rGCy'C
Cuts at: 1391 2081 2217 2264 2420 2908 2963 1391
Size: 690 136 47 156 488 55 4072
Fragments arranged by size:
4072 690 488 156 136 55 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2308 2614 2710 928
Size: 971 409 306 96 3862
Fragments arranged by size:
3862 971 409 306 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 2136 1548
Size: 588 5056
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2432 4255 136
Size: 107 122 71 631 605 760 1823 1525
Fragments arranged by size:
1823 1525 760 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2595 5639 2595
Size: 3044 2600
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2241 2908 3705 4861 1790
Size: 291 160 667 797 1156 2573
Fragments arranged by size:
2573 1156 797 667 291 160
BmgBI CAC'GTC
Cuts at: 1318 2896 1318
Size: 1578 4066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4304 1239
Size: 3065 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 2434 3769 4634 4932 2048
Size: 386 1335 865 298 2760
Fragments arranged by size:
2760 1335 865 386 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2755 4307 882
Size: 617 1256 1552 2219
Fragments arranged by size:
2219 1552 1256 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2722 2794 2914 1186
Size: 949 587 72 120 3916
Fragments arranged by size:
3916 949 587 120 72
BseYI C'CCAG_C
Cuts at: 2009 2122 2576 4847 5343 2009
Size: 113 454 2271 496 2310
Fragments arranged by size:
2310 2271 496 454 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2319 1163
Size: 573 417 166 4488
Fragments arranged by size:
4488 573 417 166
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2792 3094 3855 4883 1540
Size: 275 186 791 302 761 1028 2301
Fragments arranged by size:
2301 1028 791 761 302 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 3705 3790 4861 2217
Size: 1488 85 1071 3000
Fragments arranged by size:
3000 1488 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2217 2241 2264 2420 2908 2963
Size: 399 291 136 24 23 156 488 55
Cuts at: 2963 3705 3790 4861 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 488 399 291 156 136
85 55 24 23
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 2999 844
Size: 1891 264 3489
Fragments arranged by size:
3489 1891 264
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2694 4139 4313 65
Size: 1183 1446 1445 174 1396
Fragments arranged by size:
1446 1445 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 2744 3704 4716 2744
Size: 960 1012 3672
Fragments arranged by size:
3672 1012 960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 2113 1184
Size: 929 4715
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2248
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2595 2974 3341 3726 3743 5195 5281 5293
Size: 623 379 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 623 385 379 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2358 2420 2436 513
Size: 552 939 354 62 16 3721
Fragments arranged by size:
3721 939 552 354 62 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 2027 3160 3954 3974 2027
Size: 1133 794 20 3697
Fragments arranged by size:
3697 1133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DraIII CAC_nnn'GTG
Cuts at: 2920 2920
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2355 2433
Size: 54 1425 50 275 18 420 105 78
Cuts at: 2433 2472 2789 3091 3980 5609 8
Size: 39 317 302 889 1629 43
Fragments arranged by size:
1629 1425 889 420 317 302 275 105
78 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2789 3091 1537
Size: 275 977 302 4090
Fragments arranged by size:
4090 977 302 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2762 2840 2918 3574 1353
Size: 869 540 78 78 656 3423
Fragments arranged by size:
3423 869 656 540 78 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 2215
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3707 4304 5091 5359 1239
Size: 393 2075 597 787 268 1524
Fragments arranged by size:
2075 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 2150
Size: 5644
EcoO109I rG'GnC_Cy
Cuts at: 2068 2077 2078 2244 2904 2905 2068
Size: 9 1 166 660 1 4807
Fragments arranged by size:
4807 660 166 9 1 1
EcoRI G'AATT_C
Cuts at: 2036 2953 3331 2036
Size: 917 378 4349
Fragments arranged by size:
4349 917 378
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2935 4791 1678
Size: 1257 1856 2531
Fragments arranged by size:
2531 1856 1257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373
Size: 32 135 32 2713 32 42 32 2626
Fragments arranged by size:
2713 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2448 2969 2448
Size: 521 5123
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 2435 10
Size: 54 1425 343 420 183 3219
Fragments arranged by size:
3219 1425 420 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2435 2855 3602 3961 4120 518
Size: 648 1191 78 420 747 359 159 2042
Fragments arranged by size:
2042 1191 747 648 420 359 159 78
MspA1I CmG'CkG
Cuts at: 1606 3060 3737 4885 5130 1606
Size: 1454 677 1148 245 2120
Fragments arranged by size:
2120 1454 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2358 2436 513
Size: 552 939 354 78 3721
Fragments arranged by size:
3721 939 552 354 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2244 2244
Size: 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2077 2904 2077
Size: 827 4817
PstI C_TGCA'G
Cuts at: 830 2076 2996 830
Size: 1246 920 3478
Fragments arranged by size:
3478 1246 920
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SacI G_AGCT'C
Cuts at: 2217 2217
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SapI GCTCTTCn'nnn_
Cuts at: 2222 2222
Size: 5644
SbfI CC_TGCA'GG
Cuts at: 2076 2996 2076
Size: 920 4724
ScaI AGT'ACT
Cuts at: 2559 3892 5604 2559
Size: 1333 1712 2599
Fragments arranged by size:
2599 1712 1333
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5644
SfcI C'TryA_G
Cuts at: 826 1045 2072 2992 4127 4808 4999 826
Size: 219 1027 920 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 920 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2432 2432
Size: 5644
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 2413 3748 4649 4911 820
Size: 197 926 120 350 1335 901 262 1553
Fragments arranged by size:
1553 1335 926 901 350 262 197 120
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 2436 3083 513
Size: 552 939 354 78 647 3074
Fragments arranged by size:
3074 939 647 552 354 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2080 2385 2495 2663 2714 2869 3671
Size: 1255 500 305 110 168 51 155 802
Cuts at: 3671 3688 3841 4026 4471 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 802 500 445 305 185 168
155 153 110 51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2557 3890
Size: 274 80 33 51 1443 204 376 1333
Cuts at: 3890 5602 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1333 376 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 2822 1316
Size: 1506 4138
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2686 2686
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII
SanDI SfoI SrfI SwaI