Restriction map for
pFN21AE0861
(Circular) (Six-base) MAPSORT of: pFN21AE0861.seq Check: 2714 from: 1 to: 5533
pFN21AE0861 5533 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 1937
Size: 939 4594
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 2306 3660 4033 1563
Size: 743 1354 373 3063
Fragments arranged by size:
3063 1354 743 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3596 4980 5248 1632
Size: 1964 1384 268 1917
Fragments arranged by size:
1964 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 4432 5409 1203
Size: 3229 977 1327
Fragments arranged by size:
3229 1327 977
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2799 2831 2799
Size: 32 5501
AlwNI CAG_nnn'CTG
Cuts at: 3276 4848 3276
Size: 1572 3961
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2842 3068 3220 5329 1493
Size: 1349 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2571 4087 160
Size: 872 1539 1516 1606
Fragments arranged by size:
1606 1539 1516 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2858 1870
Size: 73 915 4545
Fragments arranged by size:
4545 915 73
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2854 4309 618
Size: 325 217 627 1067 1455 1842
Fragments arranged by size:
1842 1455 1067 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2852 1391
Size: 1461 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2635 2714 928
Size: 971 736 79 3747
Fragments arranged by size:
3747 971 736 79
BbvCI CC'TCA_GC
Cuts at: 2689 2694 2689
Size: 5 5528
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3724 3758 1903
Size: 34 50 34 1703 34 3678
Fragments arranged by size:
3678 1703 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4144 136
Size: 107 122 71 631 605 2472 1525
Fragments arranged by size:
2472 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2950 2950
Size: 5533
Bme1580I G_kGCm'C
Cuts at: 1790 3594 4750 1790
Size: 1804 1156 2573
Fragments arranged by size:
2573 1804 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3264 4222 442
Size: 416 53 83 399 24 1847 958 1753
Fragments arranged by size:
1847 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2208 1056
Size: 1152 4381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 2236 4193 1239
Size: 802 195 1957 2579
Fragments arranged by size:
2579 1957 802 195
Bpu10I CC'TnA_GC
Cuts at: 2192 2689 2694 2192
Size: 497 5 5031
Fragments arranged by size:
5031 497 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2413 2663 3658 4523 4821 2413
Size: 250 995 865 298 3125
Fragments arranged by size:
3125 995 865 298 250
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4196 882
Size: 617 2697 2219
Fragments arranged by size:
2697 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3324 493
Size: 711 2120 2702
Fragments arranged by size:
2702 2120 711
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3340 3722 4357 275
Size: 53 83 186 936 1807 382 635 1451
Fragments arranged by size:
1807 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2799 2829 1178
Size: 30 165 30 1396 30 3882
Fragments arranged by size:
3882 1396 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5533
BseYI C'CCAG_C
Cuts at: 2271 4736 5232 2271
Size: 2465 496 2572
Fragments arranged by size:
2572 2465 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4960
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2983 3744 4772 1540
Size: 275 186 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3594 3679 4750 3594
Size: 85 1071 4377
Fragments arranged by size:
4377 1071 85
BsmI GAATG_Cn'
Cuts at: 3044 3137 3044
Size: 93 5440
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2852 3594 3679 4750 1391
Size: 399 1062 742 85 1071 2174
Fragments arranged by size:
2174 1071 1062 742 399 85
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 2139 2553 3422 5152 2139
Size: 414 869 1730 2520
Fragments arranged by size:
2520 1730 869 414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2279 2888 844
Size: 1435 609 3489
Fragments arranged by size:
3489 1435 609
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2134 2769 4028 4202 65
Size: 1183 886 635 1259 174 1396
Fragments arranged by size:
1396 1259 1183 886 635 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 2106 2220 96
Size: 2010 114 3409
Fragments arranged by size:
3409 2010 114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5533
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstXI CCAn_nnnn'nTGG
Cuts at: 2638 2638
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528
Size: 891 367 385 17 1452 86 12 346
Cuts at: 5528 1972
Size: 1977
Fragments arranged by size:
1977 1452 891 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2466 2631 513
Size: 552 1401 165 3415
Fragments arranged by size:
3415 1401 552 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4794
Fragments arranged by size:
4794 683 56
BtsI GCAGTG_nn'
Cuts at: 3049 3843 3863 3049
Size: 794 20 4719
Fragments arranged by size:
4719 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2837 2837
Size: 5533
DraIII CAC_nnn'GTG
Cuts at: 2518 2518
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4540 809
Size: 507 3224 1802
Fragments arranged by size:
3224 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2376 2463
Size: 54 1425 50 275 18 495 51 87
Cuts at: 2463 2980 3869 5498 8
Size: 517 889 1629 43
Fragments arranged by size:
1629 1425 889 517 495 275 87 54
51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2980 1537
Size: 275 1168 4090
Fragments arranged by size:
4090 1168 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3463 1353
Size: 2110 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4120 4494 4640 387
Size: 1064 138 2531 374 146 1280
Fragments arranged by size:
2531 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2236 3596 4193 4980 5248 1239
Size: 393 409 195 1360 597 787 268 1524
Fragments arranged by size:
1524 1360 787 597 409 393 268 195
EcoNI CCTnn'n_nnAGG
Cuts at: 2024 2024
Size: 5533
EcoRI G'AATT_C
Cuts at: 2842 3220 2842
Size: 378 5155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2834 2728
Size: 106 5427
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 4680 1678
Size: 3002 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2388 2420 4174 4206 4248
Size: 32 135 32 816 32 1754 32 42
Cuts at: 4248 4280 1373
Size: 32 2626
Fragments arranged by size:
2626 1754 816 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 2335 3132 2335
Size: 797 4736
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2681 4647 4831 1305
Size: 1376 1966 184 2007
Fragments arranged by size:
2007 1966 1376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 2378 2465 10
Size: 54 1425 343 495 51 87 3078
Fragments arranged by size:
3078 1425 495 343 87 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 2177 2354 3491 3850 4009 518
Size: 648 972 39 177 1137 359 159 2042
Fragments arranged by size:
2042 1137 972 648 359 177 159 39
MspA1I CmG'CkG
Cuts at: 1606 2434 2474 2693 2949 3626 4774 5019 1606
Size: 828 40 219 256 677 1148 245 2120
Fragments arranged by size:
2120 1148 828 677 256 245 219 40
NcoI C'CATG_G
Cuts at: 513 1065 2466 2631 513
Size: 552 1401 165 3415
Fragments arranged by size:
3415 1401 552 165
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 2204 1052
Size: 1152 4381
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2200 2891 4436 1132
Size: 97 971 691 1545 2229
Fragments arranged by size:
2229 1545 971 691 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5533
PciI A'CATG_T
Cuts at: 4432 4432
Size: 5533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 1108
Size: 1266 4267
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2799 2831 3602 3634 5115 5147 2799
Size: 32 771 32 1481 32 3185
Fragments arranged by size:
3185 1481 771 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2213 2245 2213
Size: 32 5501
PstI C_TGCA'G
Cuts at: 830 2885 830
Size: 2055 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
PvuII CAG'CTG
Cuts at: 2434 2474 2693 2434
Size: 40 219 5274
Fragments arranged by size:
5274 219 40
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SfcI C'TryA_G
Cuts at: 826 1045 2802 2881 4016 4697 4888 826
Size: 219 1757 79 1135 681 191 1471
Fragments arranged by size:
1757 1471 1135 681 219 191 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2860 1872
Size: 988 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2392 2678 3637 4538 4800 820
Size: 197 926 449 286 959 901 262 1553
Fragments arranged by size:
1553 959 926 901 449 286 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2200 2891 1132
Size: 1068 691 3774
Fragments arranged by size:
3774 1068 691
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2322 2466 2631 2972 513
Size: 552 1257 144 165 341 3074
Fragments arranged by size:
3074 1257 552 341 165 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2315 3560 3577 3730 3915 4360 325
Size: 1255 735 1245 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1245 735 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2181 2220
Size: 274 80 33 51 1443 129 75 39
Cuts at: 2220 3779 5491 96
Size: 1559 1712 138
Fragments arranged by size:
1712 1559 1443 274 138 129 80 75
51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2438 2438
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2858 1870
Size: 988 4545
XmnI GAAnn'nnTTC
Cuts at: 3662 3662
Size: 5533
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI