Restriction map for
pFN21AE0859
(Circular) (Six-base) MAPSORT of: pFN21AE0859.seq Check: 4528 from: 1 to: 5692
pFN21AE0859 5692 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2565 2735 2565
Size: 170 5522
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2047 2582 2721 2807 2901 3755 5139 5407
Size: 415 535 139 86 94 854 1384 268
Cuts at: 5407 1632
Size: 1917
Fragments arranged by size:
1917 1384 854 535 415 268 139 94
86
AflII C'TTAA_G
Cuts at: 820 1017 2684 820
Size: 197 1667 3828
Fragments arranged by size:
3828 1667 197
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 2027 1166
Size: 861 4831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2619 3435 5007 2619
Size: 816 1572 3304
Fragments arranged by size:
3304 1572 816
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2017 3001 3227 3379 5488 1493
Size: 524 984 226 152 2109 1697
Fragments arranged by size:
2109 1697 984 524 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3013 4468 618
Size: 325 217 627 1226 1455 1842
Fragments arranged by size:
1842 1455 1226 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3011 1391
Size: 1620 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2077 2089 928
Size: 971 178 12 4531
Fragments arranged by size:
4531 971 178 12
BbvCI CC'TCA_GC
Cuts at: 2695 2695
Size: 5692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3883 3917 1903
Size: 34 50 34 1862 34 3678
Fragments arranged by size:
3678 1862 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2441 3584 4794 2441
Size: 1143 1210 3339
Fragments arranged by size:
3339 1210 1143
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 3753 4909 1790
Size: 1963 1156 2573
Fragments arranged by size:
2573 1963 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2462 3423 4381
Size: 416 53 83 399 24 1045 961 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1045 961 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 4352 1239
Size: 802 2311 2579
Fragments arranged by size:
2579 2311 802
Bpu10I CC'TnA_GC
Cuts at: 2695 2695
Size: 5692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2210 2264 4355 882
Size: 617 711 54 2091 2219
Fragments arranged by size:
2219 2091 711 617 54
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2921 2951 1178
Size: 30 165 30 1518 30 3919
Fragments arranged by size:
3919 1518 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5692
BseYI C'CCAG_C
Cuts at: 4895 5391 4895
Size: 496 5196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2557 2892 1163
Size: 573 821 335 3963
Fragments arranged by size:
3963 821 573 335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3753 3838 4909 3753
Size: 85 1071 4536
Fragments arranged by size:
4536 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3011 3753 3838 4909 1391
Size: 399 1221 742 85 1071 2174
Fragments arranged by size:
2174 1221 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 2142 3581 5311 2142
Size: 1439 1730 2523
Fragments arranged by size:
2523 1730 1439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2228 2565 2735 3047 844
Size: 1277 107 337 170 312 3489
Fragments arranged by size:
3489 1277 337 312 170 107
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 2280 4187 4361 65
Size: 1183 889 143 1907 174 1396
Fragments arranged by size:
1907 1396 1183 889 174 143
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 2872 96
Size: 2776 2916
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2396 2396
Size: 5692
BtgI C'CryG_G
Cuts at: 513 1065 2917 513
Size: 552 1852 3288
Fragments arranged by size:
3288 1852 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 3208 4002 4022 3208
Size: 794 20 4878
Fragments arranged by size:
4878 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2585 4699 809
Size: 507 1269 2114 1802
Fragments arranged by size:
2114 1802 1269 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2379 2472
Size: 54 1425 50 275 18 402 147 93
Cuts at: 2472 3139 4028 5657 8
Size: 667 889 1629 43
Fragments arranged by size:
1629 1425 889 667 402 275 147 93
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2315 2942 3622 1353
Size: 962 627 680 3423
Fragments arranged by size:
3423 962 680 627
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2047 2582 2721 2807 2901 3755
Size: 393 409 6 535 139 86 94 854
Cuts at: 3755 4352 5139 5407 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 854 787 597 535 409 393 268
139 94 86 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2813 2813
Size: 5692
EcoO109I rG'GnC_Cy
Cuts at: 2424 2424
Size: 5692
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 5692
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2827 2859 4333 4365 4407
Size: 32 135 32 1255 32 1474 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1474 1255 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2458 4806 4990 1305
Size: 1153 2348 184 2007
Fragments arranged by size:
2348 2007 1153 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2381 2474 10
Size: 54 1425 343 402 147 93 3228
Fragments arranged by size:
3228 1425 402 343 147 93 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2180 2357 2831 3650 4009 4168
Size: 648 861 153 177 474 819 359 159
Cuts at: 4168 518
Size: 2042
Fragments arranged by size:
2042 861 819 648 474 359 177 159
153
MspA1I CmG'CkG
Cuts at: 1606 2699 3108 3785 4933 5178 1606
Size: 1093 409 677 1148 245 2120
Fragments arranged by size:
2120 1148 1093 677 409 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5140
NdeI CA'TA_TG
Cuts at: 387 2247 387
Size: 1860 3832
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2203 3050 4595 1132
Size: 97 974 847 1545 2229
Fragments arranged by size:
2229 1545 974 847 97
PasI CC'CwG_GG
Cuts at: 1348 2809 1348
Size: 1461 4231
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 2981 3262 2981
Size: 281 5411
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2699 2699
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SapI GCTCTTCn'nnn_
Cuts at: 2315 2315
Size: 5692
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 3040 4175 4856 5047 826
Size: 219 1995 1135 681 191 1471
Fragments arranged by size:
1995 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2684 3796 4697 4959 820
Size: 197 926 741 1112 901 262 1553
Fragments arranged by size:
1553 1112 926 901 741 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 2203 3050 1132
Size: 1071 847 3774
Fragments arranged by size:
3774 1071 847
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 2717 1517
Size: 1200 4492
StyI C'CwwG_G
Cuts at: 513 1065 2464 3131 513
Size: 552 1399 667 3074
Fragments arranged by size:
3074 1399 667 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 3719 3736 3889 4074 4519 325
Size: 1255 723 1416 17 153 185 445 1498
Fragments arranged by size:
1498 1416 1255 723 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2872 3938
Size: 274 80 33 51 1443 207 688 1066
Cuts at: 3938 5650 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1066 688 274 207 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5652 5684 5652
Size: 32 5660
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2155 2252 2441 2155
Size: 97 189 5406
Fragments arranged by size:
5406 189 97
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI