Restriction map for
pFN21AE0854
(Circular) (Six-base) MAPSORT of: pFN21AE0854.seq Check: 6558 from: 1 to: 5701
pFN21AE0854 5701 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2702 2702
Size: 5701
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2589 3044 1937
Size: 652 455 4594
Fragments arranged by size:
4594 652 455
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 5148 5416 1632
Size: 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 2786 4600 5577 1203
Size: 1583 1814 977 1327
Fragments arranged by size:
1814 1583 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2084 1184
Size: 900 4801
AlwNI CAG_nnn'CTG
Cuts at: 2487 3444 5016 2487
Size: 957 1572 3172
Fragments arranged by size:
3172 1572 957
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 2748 3010 3236 3388 5497 1493
Size: 1255 262 226 152 2109 1697
Fragments arranged by size:
2109 1697 1255 262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 3026 1870
Size: 73 1083 4545
Fragments arranged by size:
4545 1083 73
BamHI G'GATC_C
Cuts at: 3031 3398 3031
Size: 367 5334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2507 2766 3022 4477 618
Size: 325 217 627 720 259 256 1455 1842
Fragments arranged by size:
1842 1455 720 627 325 259 256 217
BanII G_rGCy'C
Cuts at: 1391 2199 2868 3020 1391
Size: 808 669 152 4072
Fragments arranged by size:
4072 808 669 152
BbeI G_GCGC'C
Cuts at: 2770 2770
Size: 5701
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3892 3926 1903
Size: 34 1955 34 3678
Fragments arranged by size:
3678 1955 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2642 4312 136
Size: 107 122 71 631 605 970 1670 1525
Fragments arranged by size:
1670 1525 970 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2829 3762 4918 1790
Size: 1039 933 1156 2573
Fragments arranged by size:
2573 1156 1039 933
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3432 4390 442
Size: 416 53 83 399 24 2015 958 1753
Fragments arranged by size:
2015 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2162 2162
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4361 1239
Size: 3122 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4691 4989 3826
Size: 865 298 4538
Fragments arranged by size:
4538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4364 882
Size: 617 2865 2219
Fragments arranged by size:
2865 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2787 3492 493
Size: 711 1583 705 2702
Fragments arranged by size:
2702 1583 711 705
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2767 3508 3890 4525
Size: 53 83 186 936 1234 741 382 635
Cuts at: 4525 275
Size: 1451
Fragments arranged by size:
1451 1234 936 741 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4134 4532 4806 4953 5364 4134
Size: 398 274 147 411 4471
Fragments arranged by size:
4471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5476
Fragments arranged by size:
5476 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 1186
Size: 979 4722
BseYI C'CCAG_C
Cuts at: 2134 2515 4904 5400 2134
Size: 381 2389 496 2435
Fragments arranged by size:
2435 2389 496 381
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2102 2815 3011 1163
Size: 573 366 713 196 3853
Fragments arranged by size:
3853 713 573 366 196
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3151 3912 4940 1540
Size: 275 186 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3762 3847 4918 3762
Size: 85 1071 4545
Fragments arranged by size:
4545 1071 85
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2829 2868 3020 3762 3847 4918
Size: 399 409 630 39 152 742 85 1071
Cuts at: 4918 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 630 409 399 152 85
39
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 2472 3590 5320 2472
Size: 1118 1730 2853
Fragments arranged by size:
2853 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 2853 3056 844
Size: 1858 151 203 3489
Fragments arranged by size:
3489 1858 203 151
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 1394
Size: 429 1765 3507
Fragments arranged by size:
3507 1765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2779 4196 4370 65
Size: 1183 1531 1417 174 1396
Fragments arranged by size:
1531 1417 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4345 4532 1700
Size: 110 2535 187 2869
Fragments arranged by size:
2869 2535 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 3761 4773 3761
Size: 1012 4689
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5701
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 2659 1184
Size: 1475 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 2311 2311
Size: 5701
BstYI r'GATC_y
Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696
Size: 1059 367 385 17 1452 86 12 346
Cuts at: 5696 1972
Size: 1977
Fragments arranged by size:
1977 1452 1059 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 3217 4011 4031 3217
Size: 794 20 4887
Fragments arranged by size:
4887 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DraIII CAC_nnn'GTG
Cuts at: 2937 2937
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2092 4708 809
Size: 507 776 2616 1802
Fragments arranged by size:
2616 1802 776 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2643 3148 4037
Size: 54 1425 50 275 18 813 505 889
Cuts at: 4037 5666 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 813 505 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 3631 1353
Size: 800 1478 3423
Fragments arranged by size:
3423 1478 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4288 4662 4808 387
Size: 1064 138 2699 374 146 1280
Fragments arranged by size:
2699 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3764 4361 5148 5416 1239
Size: 393 2132 597 787 268 1524
Fragments arranged by size:
2132 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2748 3010 3388 2748
Size: 262 378 5061
Fragments arranged by size:
5061 378 262
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 2144 2770 4848 1678
Size: 466 626 2078 2531
Fragments arranged by size:
2531 2078 626 466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2511 2543 4342 4374 4416
Size: 32 135 32 939 32 1799 32 42
Cuts at: 4416 4448 1373
Size: 32 2626
Fragments arranged by size:
2626 1799 939 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2590 3045 3291 669
Size: 988 281 652 455 246 3079
Fragments arranged by size:
3079 988 652 455 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KasI G'GCGC_C
Cuts at: 2766 2766
Size: 5701
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2389 4815 4999 1305
Size: 1084 2426 184 2007
Fragments arranged by size:
2426 2007 1084 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3879
Fragments arranged by size:
3879 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2417 3659 4018 4177 518
Size: 648 942 309 1242 359 159 2042
Fragments arranged by size:
2042 1242 942 648 359 309 159
MspA1I CmG'CkG
Cuts at: 1606 2134 3117 3794 4942 5187 1606
Size: 528 983 677 1148 245 2120
Fragments arranged by size:
2120 1148 983 677 528 245
NarI GG'CG_CC
Cuts at: 2767 2767
Size: 5701
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5149
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 3059 4604 1132
Size: 97 1830 1545 2229
Fragments arranged by size:
2229 1830 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5701
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 1108
Size: 1278 4423
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PmlI CAC'GTG
Cuts at: 2787 2787
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 5283 5315 3770
Size: 32 1481 32 4156
Fragments arranged by size:
4156 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 3271 3271
Size: 5701
PstI C_TGCA'G
Cuts at: 830 3053 830
Size: 2223 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
PvuII CAG'CTG
Cuts at: 2134 2134
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 2588 3043 1936
Size: 652 455 4594
Fragments arranged by size:
4594 652 455
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 3049 4184 4865 5056 826
Size: 219 2004 1135 681 191 1471
Fragments arranged by size:
2004 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2768 2768
Size: 5701
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 3028 1872
Size: 1156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3805 4706 4968 820
Size: 197 926 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SspI AAT'ATT
Cuts at: 5 52 3625 5
Size: 47 3573 2081
Fragments arranged by size:
3573 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5701
StyI C'CwwG_G
Cuts at: 513 1065 3140 513
Size: 552 2075 3074
Fragments arranged by size:
3074 2075 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2139 3728 3745 3898 4083 4528 325
Size: 1255 559 1589 17 153 185 445 1498
Fragments arranged by size:
1589 1498 1255 559 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2393 3947 5659
Size: 274 80 33 51 1443 416 1554 1712
Cuts at: 5659 96
Size: 138
Fragments arranged by size:
1712 1554 1443 416 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5661 5693 5661
Size: 32 5669
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 3037 3037
Size: 5701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2653 2653
Size: 5701
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 3026 1870
Size: 1156 4545
XmnI GAAnn'nnTTC
Cuts at: 3830 3830
Size: 5701
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI