Restriction map for
pFN21AE0853
(Circular) (Six-base) MAPSORT of: pFN21AE0853.seq Check: 993 from: 1 to: 5635
pFN21AE0853 5635 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2921 2921
Size: 5635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2628 3445 278
Size: 53 83 186 936 1092 817 2468
Fragments arranged by size:
2468 1092 936 817 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2769 2978 1937
Size: 832 209 4594
Fragments arranged by size:
4594 832 209
Acc65I G'GTAC_C
Cuts at: 2385 2956 2385
Size: 571 5064
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2717 2936 3698 5082 5350 1632
Size: 1085 219 762 1384 268 1917
Fragments arranged by size:
1917 1384 1085 762 268 219
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 2229 4534 5511 1203
Size: 1026 2305 977 1327
Fragments arranged by size:
2305 1327 1026 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 2407 2837 3378 4950 2407
Size: 430 541 1572 3092
Fragments arranged by size:
3092 1572 541 430
ApaI G_GGCC'C
Cuts at: 2532 2532
Size: 5635
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2944 3170 3322 5431 1493
Size: 1451 226 152 2109 1697
Fragments arranged by size:
2109 1697 1451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2960 1870
Size: 73 1017 4545
Fragments arranged by size:
4545 1017 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2385 2721 2956 4411 618
Size: 325 217 627 598 336 235 1455 1842
Fragments arranged by size:
1842 1455 627 598 336 325 235 217
BanII G_rGCy'C
Cuts at: 1391 2532 2954 1391
Size: 1141 422 4072
Fragments arranged by size:
4072 1141 422
BbeI G_GCGC'C
Cuts at: 2725 2725
Size: 5635
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 928
Size: 971 199 4465
Fragments arranged by size:
4465 971 199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BfrBI ATG'CAT
Cuts at: 2482 2482
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 2532 3696 4852 1790
Size: 742 1164 1156 2573
Fragments arranged by size:
2573 1164 1156 742
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2344 2411 3366
Size: 416 53 83 399 24 927 67 955
Cuts at: 3366 4324 442
Size: 958 1753
Fragments arranged by size:
1753 958 955 927 416 399 83 67
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2478 4295 1239
Size: 1239 1817 2579
Fragments arranged by size:
2579 1817 1239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4298 882
Size: 617 2799 2219
Fragments arranged by size:
2799 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2625 2722 3442 3824
Size: 53 83 186 936 1092 97 720 382
Cuts at: 3824 4459 275
Size: 635 1451
Fragments arranged by size:
1451 1092 936 720 635 382 186 97
83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2451 2481 1178
Size: 30 165 30 1048 30 4332
Fragments arranged by size:
4332 1048 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2230 1186
Size: 898 146 4591
Fragments arranged by size:
4591 898 146
BseYI C'CCAG_C
Cuts at: 2134 4838 5334 2134
Size: 2704 496 2435
Fragments arranged by size:
2704 2435 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 1163
Size: 573 657 4405
Fragments arranged by size:
4405 657 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2188 2217 2424 3696 3781 4852 2188
Size: 29 207 1272 85 1071 2971
Fragments arranged by size:
2971 1272 1071 207 85 29
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2217 2424 2532 2954 3696 3781
Size: 399 398 29 207 108 422 742 85
Cuts at: 3781 4852 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 422 399 398 207 108
85 29
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 2424 3524 5254 2424
Size: 1100 1730 2805
Fragments arranged by size:
2805 1730 1100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2921 2990 844
Size: 1307 770 69 3489
Fragments arranged by size:
3489 1307 770 69
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2471 4279 4466 1700
Size: 110 661 1808 187 2869
Fragments arranged by size:
2869 1808 661 187 110
BsrGI T'GTAC_A
Cuts at: 96 2196 96
Size: 2100 3535
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2352 794
Size: 1558 4077
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstXI CCAn_nnnn'nTGG
Cuts at: 2539 2539
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2965 3332 3717 3734 5186 5272 5284 5630
Size: 993 367 385 17 1452 86 12 346
Cuts at: 5630 1972
Size: 1977
Fragments arranged by size:
1977 1452 993 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2798 2798
Size: 5635
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 513
Size: 552 939 33 4111
Fragments arranged by size:
4111 939 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2010 487
Size: 683 56 784 4112
Fragments arranged by size:
4112 784 683 56
BtsI GCAGTG_nn'
Cuts at: 3151 3945 3965 3151
Size: 794 20 4821
Fragments arranged by size:
4821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DraIII CAC_nnn'GTG
Cuts at: 2163 2352 2163
Size: 189 5446
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2597 4642 809
Size: 507 1281 2045 1802
Fragments arranged by size:
2045 1802 1281 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2637 3082 3971
Size: 54 1425 50 275 18 807 445 889
Cuts at: 3971 5600 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 807 445 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2912 3565 1353
Size: 749 810 653 3423
Fragments arranged by size:
3423 810 749 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2501 4222 4596 4742 387
Size: 1064 138 912 1721 374 146 1280
Fragments arranged by size:
1721 1280 1064 912 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2478 2717 2936 3698 4295 5082 5350
Size: 393 846 239 219 762 597 787 268
Cuts at: 5350 1239
Size: 1524
Fragments arranged by size:
1524 846 787 762 597 393 268 239
219
EcoNI CCTnn'n_nnAGG
Cuts at: 2380 2380
Size: 5635
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 2725 4782 1678
Size: 1047 2057 2531
Fragments arranged by size:
2531 2057 1047
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2451 2483 4276 4308 4350
Size: 32 135 32 879 32 1793 32 42
Cuts at: 4350 4382 1373
Size: 32 2626
Fragments arranged by size:
2626 1793 879 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2979 3225 669
Size: 988 281 1041 246 3079
Fragments arranged by size:
3079 1041 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KasI G'GCGC_C
Cuts at: 2721 2721
Size: 5635
KpnI G_GTAC'C
Cuts at: 2389 2960 2389
Size: 571 5064
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4749 4933 1305
Size: 3444 184 2007
Fragments arranged by size:
3444 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3813
Fragments arranged by size:
3813 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 3593 3952 4111 518
Size: 648 1203 1224 359 159 2042
Fragments arranged by size:
2042 1224 1203 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2711 2834 3051 3728 4876 5121 1606
Size: 528 577 123 217 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 577 528 245 217 123
NaeI GCC'GGC
Cuts at: 2473 2473
Size: 5635
NarI GG'CG_CC
Cuts at: 2722 2722
Size: 5635
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NgoMIV G'CCGG_C
Cuts at: 2471 2471
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NsiI A_TGCA'T
Cuts at: 2484 2484
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2233 2993 4538 1132
Size: 97 1004 760 1545 2229
Fragments arranged by size:
2229 1545 1004 760 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 2229 4534 2229
Size: 2305 3330
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PspOMI G'GGCC_C
Cuts at: 2528 2528
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2134 2134
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2983 4118 4799 4990 826
Size: 219 1938 1135 681 191 1471
Fragments arranged by size:
1938 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2723 2723
Size: 5635
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2004 3074 513
Size: 552 939 1070 3074
Fragments arranged by size:
3074 1070 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2363 3662 3679 3832 4017 4462 325
Size: 1255 783 1299 17 153 185 445 1498
Fragments arranged by size:
1498 1299 1255 783 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 3881 5593
Size: 274 80 33 51 1443 219 1685 1712
Cuts at: 5593 96
Size: 138
Fragments arranged by size:
1712 1685 1443 274 219 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5595 5627 5595
Size: 32 5603
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2818 2818
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 3764 3764
Size: 5635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2626 3443 276
Size: 53 83 186 936 1092 817 2468
Fragments arranged by size:
2468 1092 936 817 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI
FspAI NruI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI