Restriction map for
pFN21AE0850
(Circular) (Six-base) MAPSORT of: pFN21AE0850.seq Check: 209 from: 1 to: 5656
pFN21AE0850 5656 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2751 3719 5103 5371 1632
Size: 1119 968 1384 268 1917
Fragments arranged by size:
1917 1384 1119 968 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaLI G'TGCA_C
Cuts at: 2663 3713 4869 2663
Size: 1050 1156 3450
Fragments arranged by size:
3450 1156 1050
ApoI r'AATT_y
Cuts at: 1493 2033 2965 3191 3343 5452 1493
Size: 540 932 226 152 2109 1697
Fragments arranged by size:
2109 1697 932 540 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2483 2981 1870
Size: 73 540 498 4545
Fragments arranged by size:
4545 540 498 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2977 4432 618
Size: 325 217 627 1190 1455 1842
Fragments arranged by size:
1842 1455 1190 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2063 2508 2975 1391
Size: 672 445 467 4072
Fragments arranged by size:
4072 672 467 445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2311 2720 928
Size: 971 412 409 3864
Fragments arranged by size:
3864 971 412 409
BbvCI CC'TCA_GC
Cuts at: 2684 2684
Size: 5656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2464 2498 3847 3881 1903
Size: 34 50 34 443 34 1349 34 3678
Fragments arranged by size:
3678 1349 443 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 2466 1548
Size: 918 4738
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2667 2920 3717 4873 1790
Size: 877 253 797 1156 2573
Fragments arranged by size:
2573 1156 877 797 253
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2099 2099
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4316 1239
Size: 3077 2579
Bpu10I CC'TnA_GC
Cuts at: 2009 2684 2009
Size: 675 4981
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 2252 5650 2252
Size: 3398 2258
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2267 2297 1178
Size: 30 165 30 864 30 4537
Fragments arranged by size:
4537 864 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2686 2689 1186
Size: 1500 3 4153
Fragments arranged by size:
4153 1500 3
BseYI C'CCAG_C
Cuts at: 2940 4859 5355 2940
Size: 1919 496 3241
Fragments arranged by size:
3241 1919 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5083
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2063 2646 2667 3717 3802 4873 2063
Size: 583 21 1050 85 1071 2846
Fragments arranged by size:
2846 1071 1050 583 85 21
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2508 2646 2667 2920 2975 3717
Size: 399 273 445 138 21 253 55 742
Cuts at: 3717 3802 4873 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 445 399 273 253 138
85 55 21
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2078 4151 4325 65
Size: 1183 830 2073 174 1396
Fragments arranged by size:
2073 1396 1183 830 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 2838 96
Size: 2742 2914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 2042 2073 2261 3716 4728 2042
Size: 31 188 1455 1012 2970
Fragments arranged by size:
2970 1455 1012 188 31
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2606 794
Size: 1812 3844
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2986 3353 3738 3755 5207 5293 5305 5651
Size: 1014 367 385 17 1452 86 12 346
Cuts at: 5651 1972
Size: 1977
Fragments arranged by size:
1977 1452 1014 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2701 2701
Size: 5656
BtgI C'CryG_G
Cuts at: 513 1065 2118 2637 2907 513
Size: 552 1053 519 270 3262
Fragments arranged by size:
3262 1053 552 519 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 3172 3966 3986 3172
Size: 794 20 4842
Fragments arranged by size:
4842 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2232 2267 529
Size: 35 1668 35 3918
Fragments arranged by size:
3918 1668 35 35
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DraIII CAC_nnn'GTG
Cuts at: 2263 2263
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2279 4663 809
Size: 507 963 2384 1802
Fragments arranged by size:
2384 1802 963 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2197 2334
Size: 54 1425 50 275 18 291 76 137
Cuts at: 2334 2673 3103 3992 5621 8
Size: 339 430 889 1629 43
Fragments arranged by size:
1629 1425 889 430 339 291 275 137
76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 3586 1353
Size: 746 1487 3423
Fragments arranged by size:
3423 1487 746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2061 2061
Size: 5656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2751 3719 4316 5103 5371 1239
Size: 393 1119 968 597 787 268 1524
Fragments arranged by size:
1524 1119 968 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2548 2578 2548
Size: 30 5626
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373
Size: 32 135 32 2725 32 42 32 2626
Fragments arranged by size:
2725 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2533 748
Size: 1785 3871
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 2675 10
Size: 54 1425 343 291 552 2991
Fragments arranged by size:
2991 1425 552 343 291 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 3614 3973 4132 518
Size: 648 957 1491 359 159 2042
Fragments arranged by size:
2042 1491 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3072 3749 4897 5142 1606
Size: 1466 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2118 2637 2907 513
Size: 552 1053 519 270 3262
Fragments arranged by size:
3262 1053 552 519 270
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 3014 4559 1132
Size: 97 1785 1545 2229
Fragments arranged by size:
2229 1785 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2263 1108
Size: 1155 4501
PfoI T'CCnGG_A
Cuts at: 2153 2211 2153
Size: 58 5598
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 2578
Size: 5656
PshAI GACnn'nnGTC
Cuts at: 1613 2747 1613
Size: 1134 4522
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SacI G_AGCT'C
Cuts at: 2063 2063
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SanDI GG'GwC_CC
Cuts at: 2578 2578
Size: 5656
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2107 3004 4139 4820 5011 826
Size: 219 1062 897 1135 681 191 1471
Fragments arranged by size:
1471 1135 1062 897 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 3760 4661 4923 820
Size: 197 926 540 1277 901 262 1553
Fragments arranged by size:
1553 1277 926 901 540 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2118 2637 2907 3095 513
Size: 552 1053 519 270 188 3074
Fragments arranged by size:
3074 1053 552 519 270 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2310 2838
Size: 274 80 33 51 1443 207 126 528
Cuts at: 2838 3902 5614 96
Size: 1064 1712 138
Fragments arranged by size:
1712 1443 1064 528 274 207 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2267 2299 5616 5648 2267
Size: 32 3317 32 2275
Fragments arranged by size:
3317 2275 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2801 2801
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 2483 1943
Size: 540 5116
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 2717 3785 2717
Size: 1068 4588
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI