Restriction map for
pFN21AE0842
(Circular) (Six-base) MAPSORT of: pFN21AE0842.seq Check: 9288 from: 1 to: 5872
pFN21AE0842 5872 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2240 2915 2988 2240
Size: 675 73 5124
Fragments arranged by size:
5124 675 73
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2268 3215 1937
Size: 331 947 4594
Fragments arranged by size:
4594 947 331
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3935 5319 5587 1632
Size: 2303 1384 268 1917
Fragments arranged by size:
2303 1917 1384 268
AfeI AGC'GCT
Cuts at: 2447 3062 2447
Size: 615 5257
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 4771 5748 1203
Size: 3568 977 1327
Fragments arranged by size:
3568 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 2222 1166
Size: 1056 4816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2081 2543 2813 1184
Size: 897 462 270 4243
Fragments arranged by size:
4243 897 462 270
AlwNI CAG_nnn'CTG
Cuts at: 2109 3615 5187 2109
Size: 1506 1572 2794
Fragments arranged by size:
2794 1572 1506
ApaI G_GGCC'C
Cuts at: 2618 2673 2752 3002 3027 3166 2618
Size: 55 79 250 25 139 5324
Fragments arranged by size:
5324 250 139 79 55 25
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 3181 3407 3559 5668 1493
Size: 1688 226 152 2109 1697
Fragments arranged by size:
2109 1697 1688 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 2018 2026 2672 3001 3149 3197 1870
Size: 73 75 8 646 329 148 48 4545
Fragments arranged by size:
4545 646 329 148 75 73 48 8
BamHI G'GATC_C
Cuts at: 3202 3569 3202
Size: 367 5505
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2519 2869 3193 4648
Size: 325 217 627 372 360 350 324 1455
Cuts at: 4648 618
Size: 1842
Fragments arranged by size:
1842 1455 627 372 360 350 325 324
217
BanII G_rGCy'C
Cuts at: 1391 2130 2618 2673 2752 3002 3027 3166 3191
Size: 739 488 55 79 250 25 139 25
Cuts at: 3191 1391
Size: 4072
Fragments arranged by size:
4072 739 488 250 139 79 55 25
25
BbeI G_GCGC'C
Cuts at: 2873 2873
Size: 5872
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3075 928
Size: 971 1176 3725
Fragments arranged by size:
3725 1176 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4063 4097 1903
Size: 34 2126 34 3678
Fragments arranged by size:
3678 2126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2493 2827 2867
Size: 107 122 71 631 605 821 334 40
Cuts at: 2867 3128 4483 136
Size: 261 1355 1525
Fragments arranged by size:
1525 1355 821 631 605 334 261 122
107 71 40
BglII A'GATC_T
Cuts at: 5867 5867
Size: 5872
BlpI GC'TnA_GC
Cuts at: 2309 3289 2309
Size: 980 4892
Bme1580I G_kGCm'C
Cuts at: 1790 2162 2522 2618 2673 2752 3002 3027 3166
Size: 372 360 96 55 79 250 25 139
Cuts at: 3166 3933 5089 1790
Size: 767 1156 2573
Fragments arranged by size:
2573 1156 767 372 360 250 139 96
79 55 25
BmgBI CAC'GTC
Cuts at: 1318 2365 1318
Size: 1047 4825
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2842 3603 4561
Size: 416 53 83 399 24 1425 761 958
Cuts at: 4561 442
Size: 1753
Fragments arranged by size:
1753 1425 958 761 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 4532 1239
Size: 920 2373 2579
Fragments arranged by size:
2579 2373 920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3997 4862 5160 3997
Size: 865 298 4709
Fragments arranged by size:
4709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4535 882
Size: 617 3036 2219
Fragments arranged by size:
3036 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2272 3663 493
Size: 711 1068 1391 2702
Fragments arranged by size:
2702 1391 1068 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2870 3679 4061 4696
Size: 53 83 186 936 1337 809 382 635
Cuts at: 4696 275
Size: 1451
Fragments arranged by size:
1451 1337 936 809 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4305 4703 4977 5124 5535 4305
Size: 398 274 147 411 4642
Fragments arranged by size:
4642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5647
Fragments arranged by size:
5647 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5872
BseYI C'CCAG_C
Cuts at: 2129 2515 2785 3124 5075 5571 2129
Size: 386 270 339 1951 496 2430
Fragments arranged by size:
2430 1951 496 386 339 270
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 1163
Size: 573 765 4534
Fragments arranged by size:
4534 765 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2124 2468 2759 2989 3322 4083
Size: 275 186 123 344 291 230 333 761
Cuts at: 4083 5111 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 344 333 291 275 230
186 123
BsiHKAI G_wGCw'C
Cuts at: 2382 3933 4018 5089 2382
Size: 1551 85 1071 3165
Fragments arranged by size:
3165 1551 1071 85
BsmI GAATG_Cn'
Cuts at: 3383 3476 3383
Size: 93 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2162 2382 2522 2618 2673 2752
Size: 399 340 32 220 140 96 55 79
Cuts at: 2752 3002 3027 3166 3191 3933 4018 5089 1391
Size: 250 25 139 25 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 250 220 140
139 96 85 79 55 32 25 25
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 2433 3761 5491 2433
Size: 1328 1730 2814
Fragments arranged by size:
2814 1730 1328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2240 2304 2313 2915 2988 3151 3227 844
Size: 1396 64 9 602 73 163 76 3489
Fragments arranged by size:
3489 1396 602 163 76 73 64 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2238 2724 3075 3759 1394
Size: 429 415 486 351 684 3507
Fragments arranged by size:
3507 684 486 429 415 351
BsrDI GCAATG_nn'
Cuts at: 65 1248 4367 4541 65
Size: 1183 3119 174 1396
Fragments arranged by size:
3119 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4516 4703 1700
Size: 110 2706 187 2869
Fragments arranged by size:
2869 2706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 2620 2699 2731 2765 2767 2769 2882 1925
Size: 695 79 32 34 2 2 113 4915
Fragments arranged by size:
4915 695 113 79 34 32 2 2
BssSI C'ACGA_G
Cuts at: 3113 3932 4944 3113
Size: 819 1012 4041
Fragments arranged by size:
4041 1012 819
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2928 794
Size: 2134 3738
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 2227 1184
Size: 1043 4829
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2191 2572 2137
Size: 54 381 5437
Fragments arranged by size:
5437 381 54
BstYI r'GATC_y
Cuts at: 1972 3202 3569 3954 3971 5423 5509 5521 5867
Size: 1230 367 385 17 1452 86 12 346
Cuts at: 5867 1972
Size: 1977
Fragments arranged by size:
1977 1452 1230 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2184 2873 513
Size: 552 939 150 30 689 3512
Fragments arranged by size:
3512 939 689 552 150 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2647 487
Size: 683 56 1421 3712
Fragments arranged by size:
3712 1421 683 56
BtsI GCAGTG_nn'
Cuts at: 3388 4182 4202 3388
Size: 794 20 5058
Fragments arranged by size:
5058 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2020 2176 4879 809
Size: 507 704 156 2703 1802
Fragments arranged by size:
2703 1802 704 507 156
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2151 2181
Size: 54 1425 50 275 18 216 105 30
Cuts at: 2181 2289 2328 2465 2562 2756 2986 3319 4208
Size: 108 39 137 97 194 230 333 889
Cuts at: 4208 5837 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 333 275 230 216 194
137 108 105 97 54 50 43 39
30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2465 2756 2986 3319 1537
Size: 275 653 291 230 333 4090
Fragments arranged by size:
4090 653 333 291 275 230
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 2654 2807 3802 1353
Size: 1034 267 153 995 3423
Fragments arranged by size:
3423 1034 995 267 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4459 4833 4979 387
Size: 1064 138 2870 374 146 1280
Fragments arranged by size:
2870 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 3935 4532 5319 5587 1239
Size: 393 527 1776 597 787 268 1524
Fragments arranged by size:
1776 1524 787 597 527 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2143 2585 2614 2669 2860 2998 3023 3155 3162
Size: 442 29 55 191 138 25 132 7
Cuts at: 3162 3163 2143
Size: 1 4852
Fragments arranged by size:
4852 442 191 138 132 55 29 25
7 1
EcoRI G'AATT_C
Cuts at: 3181 3559 3181
Size: 378 5494
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 2012 2288 2449 2488 2516 2537 2873 3064
Size: 334 276 161 39 28 21 336 191
Cuts at: 3064 5019 1678
Size: 1955 2531
Fragments arranged by size:
2531 1955 336 334 276 191 161 39
28 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373
Size: 32 135 32 2941 32 42 32 2626
Fragments arranged by size:
2941 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2278 2893 3216 3462 669
Size: 988 281 340 615 323 246 3079
Fragments arranged by size:
3079 988 615 340 323 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KasI G'GCGC_C
Cuts at: 2869 2869
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4986 5170 1305
Size: 3681 184 2007
Fragments arranged by size:
3681 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2153 2330 2564 10
Size: 54 1425 343 321 177 234 3318
Fragments arranged by size:
3318 1425 343 321 234 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2222 3830 4189 4348 518
Size: 648 1023 33 1608 359 159 2042
Fragments arranged by size:
2042 1608 1023 648 359 159 33
MspA1I CmG'CkG
Cuts at: 1606 2470 2839 2866 2875 2928 3009 3128 3288
Size: 864 369 27 9 53 81 119 160
Cuts at: 3288 3965 5113 5358 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 864 677 369 245 160 119
81 53 27 9
NarI GG'CG_CC
Cuts at: 2870 2870
Size: 5872
NcoI C'CATG_G
Cuts at: 513 1065 2004 2184 513
Size: 552 939 180 4201
Fragments arranged by size:
4201 939 552 180
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 2465 2986 3319 2465
Size: 521 333 5018
Fragments arranged by size:
5018 521 333
NspI r_CATG'y
Cuts at: 1132 1229 3230 4775 1132
Size: 97 2001 1545 2229
Fragments arranged by size:
2229 2001 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2039 3159 1348
Size: 691 1120 4061
Fragments arranged by size:
4061 1120 691
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2349 1108
Size: 1241 4631
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2585 3155 2143
Size: 442 570 4860
Fragments arranged by size:
4860 570 442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PspOMI G'GGCC_C
Cuts at: 2614 2669 2748 2998 3023 3162 2614
Size: 55 79 250 25 139 5324
Fragments arranged by size:
5324 250 139 79 55 25
PstI C_TGCA'G
Cuts at: 830 3042 3092 3224 830
Size: 2212 50 132 3478
Fragments arranged by size:
3478 2212 132 50
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
PvuII CAG'CTG
Cuts at: 2839 2928 2839
Size: 89 5783
SacII CC_GC'GG
Cuts at: 2876 2876
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SanDI GG'GwC_CC
Cuts at: 2585 3155 2585
Size: 570 5302
SapI GCTCTTCn'nnn_
Cuts at: 2387 2654 2807 2387
Size: 267 153 5452
Fragments arranged by size:
5452 267 153
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SexAI A'CCwGG_T
Cuts at: 2145 2145
Size: 5872
SfcI C'TryA_G
Cuts at: 826 1045 3038 3088 3220 4355 5036 5227 826
Size: 219 1993 50 132 1135 681 191 1471
Fragments arranged by size:
1993 1471 1135 681 219 191 132 50
SfiI GGCCn_nnn'nGGCC
Cuts at: 3128 3128
Size: 5872
SfoI GGC'GCC
Cuts at: 2871 2871
Size: 5872
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 2674 3003 3199 1872
Size: 802 329 196 4545
Fragments arranged by size:
4545 802 329 196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3976 4877 5139 820
Size: 197 926 2033 901 262 1553
Fragments arranged by size:
2033 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SrfI GCCC'GGGC
Cuts at: 2674 3003 2674
Size: 329 5543
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 2004 2184 2343 2490 3311 513
Size: 552 939 180 159 147 821 3074
Fragments arranged by size:
3074 939 821 552 180 159 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 3899 3916 4069 4254 4699 325
Size: 1255 558 1761 17 153 185 445 1498
Fragments arranged by size:
1761 1498 1255 558 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4118 5830 96
Size: 274 80 33 51 1443 2141 1712 138
Fragments arranged by size:
2141 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5832 5864 5832
Size: 32 5840
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2191
Size: 5872
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 2672 3001 3197 1870
Size: 802 329 196 4545
Fragments arranged by size:
4545 802 329 196
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SwaI