Restriction map for
pFN21AE0841
(Circular) (Six-base) MAPSORT of: pFN21AE0841.seq Check: 373 from: 1 to: 5731
pFN21AE0841 5731 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 3074 1937
Size: 885 252 4594
Fragments arranged by size:
4594 885 252
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3794 5178 5446 1632
Size: 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 2331 3474 5046 2331
Size: 1143 1572 3016
Fragments arranged by size:
3016 1572 1143
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 2309 2451 2883 3040 3266 3418 5527 1493
Size: 816 142 432 157 226 152 2109 1697
Fragments arranged by size:
2109 1697 816 432 226 157 152 142
AseI AT'TA_AT
Cuts at: 160 1032 2837 4285 160
Size: 872 1805 1448 1606
Fragments arranged by size:
1805 1606 1448 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 3056 1870
Size: 73 151 962 4545
Fragments arranged by size:
4545 962 151 73
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3052 4507 618
Size: 325 217 627 1265 1455 1842
Fragments arranged by size:
1842 1455 1265 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3050 1391
Size: 1659 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2222 4342 136
Size: 107 122 71 631 605 550 2120 1525
Fragments arranged by size:
2120 1525 631 605 550 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 3792 4948 1790
Size: 2002 1156 2573
Fragments arranged by size:
2573 2002 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3462 4420 442
Size: 416 53 83 399 24 2045 958 1753
Fragments arranged by size:
2045 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2552 4391 1239
Size: 1313 1839 2579
Fragments arranged by size:
2579 1839 1313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3856 4721 5019 3856
Size: 865 298 4568
Fragments arranged by size:
4568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2487 3522 493
Size: 711 1283 1035 2702
Fragments arranged by size:
2702 1283 1035 711
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5506
Fragments arranged by size:
5506 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5731
BseYI C'CCAG_C
Cuts at: 2592 4934 5430 2592
Size: 2342 496 2893
Fragments arranged by size:
2893 2342 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2174 1163
Size: 573 318 120 4720
Fragments arranged by size:
4720 573 318 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2551 3792 3877 4948 2551
Size: 1241 85 1071 3334
Fragments arranged by size:
3334 1241 1071 85
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2551 3050 3792 3877 4948 1391
Size: 399 761 499 742 85 1071 2174
Fragments arranged by size:
2174 1071 761 742 499 399 85
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3086 844
Size: 2242 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2211 4226 4400 65
Size: 1183 963 2015 174 1396
Fragments arranged by size:
2015 1396 1183 963 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 2106 96
Size: 2010 3721
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 2814 1184
Size: 1630 4101
BstYI r'GATC_y
Cuts at: 1972 2060 3061 3428 3813 3830 5282 5368 5380
Size: 88 1001 367 385 17 1452 86 12
Cuts at: 5380 5726 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1001 385 367 346 88 86
17 12
BstZ17I GTA'TAC
Cuts at: 2823 2823
Size: 5731
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4992
Fragments arranged by size:
4992 683 56
BtsI GCAGTG_nn'
Cuts at: 3247 4041 4061 3247
Size: 794 20 4917
Fragments arranged by size:
4917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 2994 3035 2994
Size: 41 5690
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2748 3178 4067
Size: 54 1425 50 275 18 918 430 889
Cuts at: 4067 5696 8
Size: 1629 43
Fragments arranged by size:
1629 1425 918 889 430 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 2359 3661 1353
Size: 972 34 1302 3423
Fragments arranged by size:
3423 1302 972 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4318 4692 4838 387
Size: 1064 138 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2552 3794 4391 5178 5446 1239
Size: 393 920 1242 597 787 268 1524
Fragments arranged by size:
1524 1242 920 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2091 2091
Size: 5731
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 4878 1678
Size: 3200 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2464 2496 2920 2952 4372
Size: 32 135 32 892 32 424 32 1420
Cuts at: 4372 4404 4446 4478 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1420 892 424 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2222 2873 4845 5029 1305
Size: 917 651 1972 184 2007
Fragments arranged by size:
2007 1972 917 651 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2750 10
Size: 54 1425 343 918 2991
Fragments arranged by size:
2991 1425 918 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 3689 4048 4207 518
Size: 648 1296 1227 359 159 2042
Fragments arranged by size:
2042 1296 1227 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2188 2493 3147 3824 4972 5217 1606
Size: 582 305 654 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 654 582 305 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5179
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 3089 4634 1132
Size: 97 1860 1545 2229
Fragments arranged by size:
2229 1860 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2779 1348
Size: 1431 4300
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5731
PfoI T'CCnGG_A
Cuts at: 2468 2468
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2091 2091
Size: 5731
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 2736 2907 3301 2736
Size: 171 394 5166
Fragments arranged by size:
5166 394 171
PstI C_TGCA'G
Cuts at: 830 3083 830
Size: 2253 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2188 2188
Size: 5731
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SanDI GG'GwC_CC
Cuts at: 2091 2091
Size: 5731
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 3079 4214 4895 5086 826
Size: 219 2034 1135 681 191 1471
Fragments arranged by size:
2034 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2096 3058 1872
Size: 224 962 4545
Fragments arranged by size:
4545 962 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3835 4736 4998 820
Size: 197 926 1892 901 262 1553
Fragments arranged by size:
1892 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 2702 152
Size: 2550 3181
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 2948 3170 513
Size: 552 1883 222 3074
Fragments arranged by size:
3074 1883 552 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2584 3758 3775 3928 4113 4558 325
Size: 1255 1004 1174 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1174 1004 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2315 3977
Size: 274 80 33 51 1443 129 209 1662
Cuts at: 3977 5689 96
Size: 1712 138
Fragments arranged by size:
1712 1662 1443 274 209 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5691 5723 5691
Size: 32 5699
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 2094 3056 1870
Size: 224 962 4545
Fragments arranged by size:
4545 962 224
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI