Restriction map for
pFN21AE0838
(Circular) (Six-base) MAPSORT of: pFN21AE0838.seq Check: 5409 from: 1 to: 5632
pFN21AE0838 5632 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2315 2315
Size: 5632
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 2390 3695 5079 5347 1632
Size: 719 39 1305 1384 268 1917
Fragments arranged by size:
1917 1384 1305 719 268 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 2906 4531 5508 1203
Size: 1703 1625 977 1327
Fragments arranged by size:
1703 1625 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaLI G'TGCA_C
Cuts at: 2393 3689 4845 2393
Size: 1296 1156 3180
Fragments arranged by size:
3180 1296 1156
ApoI r'AATT_y
Cuts at: 1493 2051 2169 2941 3167 3319 5428 1493
Size: 558 118 772 226 152 2109 1697
Fragments arranged by size:
2109 1697 772 558 226 152 118
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
BamHI G'GATC_C
Cuts at: 2400 2962 3329 2400
Size: 562 367 4703
Fragments arranged by size:
4703 562 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2953 4408 618
Size: 325 217 627 1166 1455 1842
Fragments arranged by size:
1842 1455 1166 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2951 1391
Size: 1560 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2311 928
Size: 971 412 4249
Fragments arranged by size:
4249 971 412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2072 3524 4734 2072
Size: 1452 1210 2970
Fragments arranged by size:
2970 1452 1210
BclI T'GATC_A
Cuts at: 1548 2178 1548
Size: 630 5002
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2621 5627 2621
Size: 3006 2626
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2397 3693 4849 1790
Size: 607 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 607
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2067 4292 1239
Size: 828 2225 2579
Fragments arranged by size:
2579 2225 828
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2667 4295 882
Size: 617 1168 1628 2219
Fragments arranged by size:
2219 1628 1168 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5632
BseYI C'CCAG_C
Cuts at: 2407 2678 4835 5331 2407
Size: 271 2157 496 2708
Fragments arranged by size:
2708 2157 496 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5059
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 3693 3778 4849 2397
Size: 1296 85 1071 3180
Fragments arranged by size:
3180 1296 1071 85
BsmI GAATG_Cn'
Cuts at: 2904 3143 3236 2904
Size: 239 93 5300
Fragments arranged by size:
5300 239 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 2951 3693 3778 4849 1391
Size: 399 607 554 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 607 554 399 85
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2315 2987 844
Size: 1471 672 3489
Fragments arranged by size:
3489 1471 672
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2082 4127 4301 65
Size: 1183 834 2045 174 1396
Fragments arranged by size:
2045 1396 1183 834 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2663 4276 4463 1700
Size: 110 853 1613 187 2869
Fragments arranged by size:
2869 1613 853 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2400 2621 2962 3329 3714 3731 5183 5269
Size: 428 221 341 367 385 17 1452 86
Cuts at: 5269 5281 5627 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 428 385 367 346 341 221
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5080
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2868 2936 2868
Size: 68 5564
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3079 3968 5597
Size: 54 1425 50 275 18 1249 889 1629
Cuts at: 5597 8
Size: 43
Fragments arranged by size:
1629 1425 1249 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 1353
Size: 2209 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2351 2390 3695 4292 5079 5347
Size: 393 435 284 39 1305 597 787 268
Cuts at: 5347 1239
Size: 1524
Fragments arranged by size:
1524 1305 787 597 435 393 284 268
39
EcoRI G'AATT_C
Cuts at: 2051 2941 3319 2051
Size: 890 378 4364
Fragments arranged by size:
4364 890 378
EcoRV GAT'ATC
Cuts at: 2601 2601
Size: 5632
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 4779 1678
Size: 3101 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373
Size: 32 135 32 2701 32 42 32 2626
Fragments arranged by size:
2701 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2095 2137 2976 3222 669
Size: 988 281 157 42 839 246 3079
Fragments arranged by size:
3079 988 839 281 246 157 42
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 2484 2574 3231 2484
Size: 90 657 4885
Fragments arranged by size:
4885 657 90
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3810
Fragments arranged by size:
3810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3590 3949 4108 518
Size: 648 2424 359 159 2042
Fragments arranged by size:
2424 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3048 3725 4873 5118 1606
Size: 1442 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5080
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2910 2990 4535 1132
Size: 97 1681 80 1545 2229
Fragments arranged by size:
2229 1681 1545 97 80
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 2906 4531 2906
Size: 1625 4007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2891 1613
Size: 1278 4354
PsiI TTA'TAA
Cuts at: 2033 3202 2033
Size: 1169 4463
PstI C_TGCA'G
Cuts at: 830 2020 2984 830
Size: 1190 964 3478
Fragments arranged by size:
3478 1190 964
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 2214 2457 3880 5592 2214
Size: 243 1423 1712 2254
Fragments arranged by size:
2254 1712 1423 243
SfcI C'TryA_G
Cuts at: 826 1045 2016 2980 4115 4796 4987 826
Size: 219 971 964 1135 681 191 1471
Fragments arranged by size:
1471 1135 971 964 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4637 4899 820
Size: 197 926 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 2089 152
Size: 1937 3695
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 2067 3556 5
Size: 47 2015 1489 2081
Fragments arranged by size:
2081 2015 1489 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2838 3071 513
Size: 552 1773 233 3074
Fragments arranged by size:
3074 1773 552 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2212 2455 3878
Size: 274 80 33 51 1443 235 243 1423
Cuts at: 3878 5590 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1423 274 243 235 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2361 2428 2361
Size: 67 5565
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI