Restriction map for
pFN21AE0824
(Circular) (Six-base) MAPSORT of: pFN21AE0824.seq Check: 4427 from: 1 to: 5509
pFN21AE0824 5509 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3319 278
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2852 1937
Size: 915 4594
Acc65I G'GTAC_C
Cuts at: 2830 2830
Size: 5509
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3572 4956 5224 1632
Size: 1940 1384 268 1917
Fragments arranged by size:
1940 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 2085 4408 5385 1203
Size: 882 2323 977 1327
Fragments arranged by size:
2323 1327 977 882
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 4238 4238
Size: 5509
AleI CACnn'nnGTG
Cuts at: 1166 2610 1166
Size: 1444 4065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5509
AlwNI CAG_nnn'CTG
Cuts at: 3252 4824 3252
Size: 1572 3937
ApaI G_GGCC'C
Cuts at: 2388 2388
Size: 5509
ApaLI G'TGCA_C
Cuts at: 2296 3566 4722 2296
Size: 1270 1156 3083
Fragments arranged by size:
3083 1270 1156
ApoI r'AATT_y
Cuts at: 1493 2415 2818 3044 3196 5305 1493
Size: 922 403 226 152 2109 1697
Fragments arranged by size:
2109 1697 922 403 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4063 160
Size: 872 3031 1606
Fragments arranged by size:
3031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2388 2834 1870
Size: 73 445 446 4545
Fragments arranged by size:
4545 446 445 73
BamHI G'GATC_C
Cuts at: 2839 3206 2839
Size: 367 5142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2771 2830 4285 618
Size: 325 217 627 984 59 1455 1842
Fragments arranged by size:
1842 1455 984 627 325 217 59
BanII G_rGCy'C
Cuts at: 1391 2388 2828 1391
Size: 997 440 4072
Fragments arranged by size:
4072 997 440
BbeI G_GCGC'C
Cuts at: 2775 2775
Size: 5509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3700 3734 1903
Size: 34 1763 34 3678
Fragments arranged by size:
3678 1763 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3401 4611 3401
Size: 1210 4299
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BfrBI ATG'CAT
Cuts at: 2166 2166
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4120 136
Size: 107 122 71 631 605 2448 1525
Fragments arranged by size:
2448 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5504 5504
Size: 5509
BlpI GC'TnA_GC
Cuts at: 2926 2926
Size: 5509
Bme1580I G_kGCm'C
Cuts at: 1790 2300 2388 3570 4726 1790
Size: 510 88 1182 1156 2573
Fragments arranged by size:
2573 1182 1156 510 88
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3240 4198 442
Size: 416 53 83 399 24 1823 958 1753
Fragments arranged by size:
1823 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5509
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2775 2775
Size: 5509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2308 4169 1239
Size: 1010 59 1861 2579
Fragments arranged by size:
2579 1861 1010 59
Bpu10I CC'TnA_GC
Cuts at: 2760 2760
Size: 5509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 3634 4499 4797 2414
Size: 1220 865 298 3126
Fragments arranged by size:
3126 1220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4172 882
Size: 617 2673 2219
Fragments arranged by size:
2673 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3300 493
Size: 711 2096 2702
Fragments arranged by size:
2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2772 3316 3698 4333
Size: 53 83 186 936 1239 544 382 635
Cuts at: 4333 275
Size: 1451
Fragments arranged by size:
1451 1239 936 635 544 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3942 4340 4614 4761 5172 3942
Size: 398 274 147 411 4279
Fragments arranged by size:
4279 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5284
Fragments arranged by size:
5284 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2590 2693 1186
Size: 1404 103 4002
Fragments arranged by size:
4002 1404 103
BseYI C'CCAG_C
Cuts at: 4712 5208 4712
Size: 496 5013
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4936
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2959 3720 4748 1540
Size: 275 186 958 761 1028 2301
Fragments arranged by size:
2301 1028 958 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2300 2788 3570 3655 4726 2300
Size: 488 782 85 1071 3083
Fragments arranged by size:
3083 1071 782 488 85
BsmI GAATG_Cn'
Cuts at: 2761 3020 3113 2761
Size: 259 93 5157
Fragments arranged by size:
5157 259 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2300 2388 2788 2828 3570 3655 4726
Size: 399 510 88 400 40 742 85 1071
Cuts at: 4726 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 510 400 399 88 85
40
BspEI T'CCGG_A
Cuts at: 5172 5172
Size: 5509
BspHI T'CATG_A
Cuts at: 2280 3398 5128 2280
Size: 1118 1730 2661
Fragments arranged by size:
2661 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2603 2864 844
Size: 1759 261 3489
Fragments arranged by size:
3489 1759 261
BsrBI CCG'CTC
Cuts at: 1394 1823 3396 1394
Size: 429 1573 3507
Fragments arranged by size:
3507 1573 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4004 4178 65
Size: 1183 2756 174 1396
Fragments arranged by size:
2756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4153 4340 1700
Size: 110 2343 187 2869
Fragments arranged by size:
2869 2343 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5509
BssSI C'ACGA_G
Cuts at: 3569 4581 3569
Size: 1012 4497
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5509
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2119 2011
Size: 108 5401
BstYI r'GATC_y
Cuts at: 1972 2556 2839 3206 3591 3608 5060 5146 5158
Size: 584 283 367 385 17 1452 86 12
Cuts at: 5158 5504 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 584 385 367 346 283 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2640 513
Size: 552 1575 3382
Fragments arranged by size:
3382 1575 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4770
Fragments arranged by size:
4770 683 56
BtsI GCAGTG_nn'
Cuts at: 2786 3025 3819 3839 2786
Size: 239 794 20 4456
Fragments arranged by size:
4456 794 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2813 2813
Size: 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2022 4516 809
Size: 507 706 2494 1802
Fragments arranged by size:
2494 1802 706 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2247 2956
Size: 54 1425 50 275 18 243 174 709
Cuts at: 2956 3845 5474 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 709 275 243 174 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2956 1537
Size: 275 1144 4090
Fragments arranged by size:
4090 1144 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 2630 3439 1353
Size: 1058 219 809 3423
Fragments arranged by size:
3423 1058 809 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4096 4470 4616 387
Size: 1064 138 2507 374 146 1280
Fragments arranged by size:
2507 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2308 3572 4169 4956 5224 1239
Size: 393 617 59 1264 597 787 268 1524
Fragments arranged by size:
1524 1264 787 617 597 393 268 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2236 2236
Size: 5509
EcoO109I rG'GnC_Cy
Cuts at: 2385 2385
Size: 5509
EcoRI G'AATT_C
Cuts at: 2818 3196 2818
Size: 378 5131
FspI TGC'GCA
Cuts at: 4015 4015
Size: 5509
HaeII r_GCGC'y
Cuts at: 1678 2775 4656 1678
Size: 1097 1881 2531
Fragments arranged by size:
2531 1881 1097
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2293 2325 2731 2763 4150
Size: 32 135 32 721 32 406 32 1387
Cuts at: 4150 4182 4224 4256 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1387 721 406 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 2853 3099 669
Size: 988 281 520 395 246 3079
Fragments arranged by size:
3079 988 520 395 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5509
HpaI GTT'AAC
Cuts at: 2458 3099 2458
Size: 641 4868
KasI G'GCGC_C
Cuts at: 2771 2771
Size: 5509
KpnI G_GTAC'C
Cuts at: 2834 2834
Size: 5509
MfeI C'AATT_G
Cuts at: 3108 3108
Size: 5509
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 2479 2599 4623 4807 1305
Size: 819 355 120 2024 184 2007
Fragments arranged by size:
2024 2007 819 355 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2249 10
Size: 54 1425 343 243 174 3270
Fragments arranged by size:
3270 1425 343 243 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2610 2639 3467 3826 3985 518
Size: 648 918 526 29 828 359 159 2042
Fragments arranged by size:
2042 918 828 648 526 359 159 29
MspA1I CmG'CkG
Cuts at: 1606 2925 3602 4750 4995 1606
Size: 1319 677 1148 245 2120
Fragments arranged by size:
2120 1319 1148 677 245
NarI GG'CG_CC
Cuts at: 2772 2772
Size: 5509
NcoI C'CATG_G
Cuts at: 513 1065 2640 513
Size: 552 1575 3382
Fragments arranged by size:
3382 1575 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5509
NotI GC'GGCC_GC
Cuts at: 2956 2956
Size: 5509
NsiI A_TGCA'T
Cuts at: 2168 2168
Size: 5509
NspI r_CATG'y
Cuts at: 1132 1229 2089 2354 2533 2867 4412 1132
Size: 97 860 265 179 334 1545 2229
Fragments arranged by size:
2229 1545 860 334 265 179 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5509
PciI A'CATG_T
Cuts at: 2085 4408 2085
Size: 2323 3186
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5509
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 5091 5123 3578
Size: 32 1481 32 3964
Fragments arranged by size:
3964 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5509
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PspOMI G'GGCC_C
Cuts at: 2384 2384
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2793 2861 830
Size: 1963 68 3478
Fragments arranged by size:
3478 1963 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
SalI G'TCGA_C
Cuts at: 1936 2851 1936
Size: 915 4594
SbfI CC_TGCA'GG
Cuts at: 2861 2861
Size: 5509
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2202 2743 2789 2857 3992 4673 4864
Size: 219 1157 541 46 68 1135 681 191
Cuts at: 4864 826
Size: 1471
Fragments arranged by size:
1471 1157 1135 681 541 219 191 68
46
SfoI GGC'GCC
Cuts at: 2773 2773
Size: 5509
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5509
SmaI CCC'GGG
Cuts at: 1872 2390 2836 1872
Size: 518 446 4545
Fragments arranged by size:
4545 518 446
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 3613 4514 4776 820
Size: 197 926 486 1184 901 262 1553
Fragments arranged by size:
1553 1184 926 901 486 262 197
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2867 1132
Size: 1735 3774
SspI AAT'ATT
Cuts at: 5 52 3433 5
Size: 47 3381 2081
Fragments arranged by size:
3381 2081 47
StuI AGG'CCT
Cuts at: 1517 2585 1517
Size: 1068 4441
StyI C'CwwG_G
Cuts at: 513 1065 2640 2948 513
Size: 552 1575 308 3074
Fragments arranged by size:
3074 1575 552 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 3553 3706 3891 4336 325
Size: 1255 1956 17 153 185 445 1498
Fragments arranged by size:
1956 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 2268 3755
Size: 274 80 33 51 1443 75 216 1487
Cuts at: 3755 5467 96
Size: 1712 138
Fragments arranged by size:
1712 1487 1443 274 216 138 80 75
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5469 5501 5469
Size: 32 5477
Tth111I GACn'n_nGTC
Cuts at: 1316 2800 1316
Size: 1484 4025
XbaI T'CTAG_A
Cuts at: 2845 2845
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5509
XmaI C'CCGG_G
Cuts at: 1870 2388 2834 1870
Size: 518 446 4545
Fragments arranged by size:
4545 518 446
XmnI GAAnn'nnTTC
Cuts at: 3638 3638
Size: 5509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3317 276
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI