Restriction map for
pFN21AE0823
(Circular) (Six-base) MAPSORT of: pFN21AE0823.seq Check: 5897 from: 1 to: 5404
pFN21AE0823 5404 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2687 2687
Size: 5404
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 1937
Size: 810 4594
Acc65I G'GTAC_C
Cuts at: 2420 2725 2420
Size: 305 5099
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 3467 4851 5119 1632
Size: 947 888 1384 268 1917
Fragments arranged by size:
1917 1384 947 888 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5207
AflIII A'CryG_T
Cuts at: 1203 4303 5280 1203
Size: 3100 977 1327
Fragments arranged by size:
3100 1327 977
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 4133 4133
Size: 5404
AleI CACnn'nnGTG
Cuts at: 1166 2177 1166
Size: 1011 4393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2408 1184
Size: 1224 4180
AlwNI CAG_nnn'CTG
Cuts at: 2248 3147 4719 2248
Size: 899 1572 2933
Fragments arranged by size:
2933 1572 899
ApaLI G'TGCA_C
Cuts at: 2488 3461 4617 2488
Size: 973 1156 3275
Fragments arranged by size:
3275 1156 973
ApoI r'AATT_y
Cuts at: 1493 2713 2939 3091 5200 1493
Size: 1220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1220 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3958 160
Size: 872 2926 1606
Fragments arranged by size:
2926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2729 1870
Size: 73 786 4545
Fragments arranged by size:
4545 786 73
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2420 2725 4180 618
Size: 325 217 627 633 305 1455 1842
Fragments arranged by size:
1842 1455 633 627 325 305 217
BanII G_rGCy'C
Cuts at: 1391 2723 1391
Size: 1332 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 928
Size: 971 145 4288
Fragments arranged by size:
4288 971 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3595 3629 1903
Size: 34 1658 34 3678
Fragments arranged by size:
3678 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 2139 1548
Size: 591 4813
BfrBI ATG'CAT
Cuts at: 2619 2619
Size: 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2258 4015 136
Size: 107 122 71 631 605 586 1757 1525
Fragments arranged by size:
1757 1525 631 605 586 122 107 71
BglII A'GATC_T
Cuts at: 5399 5399
Size: 5404
BlpI GC'TnA_GC
Cuts at: 2821 2821
Size: 5404
Bme1580I G_kGCm'C
Cuts at: 1790 2492 3465 4621 1790
Size: 702 973 1156 2573
Fragments arranged by size:
2573 1156 973 702
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 4093 442
Size: 416 53 83 399 24 1718 958 1753
Fragments arranged by size:
1753 1718 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 4064 1239
Size: 1161 1664 2579
Fragments arranged by size:
2579 1664 1161
Bpu10I CC'TnA_GC
Cuts at: 2192 2192
Size: 5404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3529 4394 4692 3529
Size: 865 298 4241
Fragments arranged by size:
4241 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4067 882
Size: 617 2568 2219
Fragments arranged by size:
2568 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2487 3195 493
Size: 711 1283 708 2702
Fragments arranged by size:
2702 1283 711 708
BsaBI GATnn'nnATC
Cuts at: 5398 5398
Size: 5404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3211 3593 4228 275
Size: 53 83 186 936 1678 382 635 1451
Fragments arranged by size:
1678 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3837 4235 4509 4656 5067 3837
Size: 398 274 147 411 4174
Fragments arranged by size:
4174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2398 2428 1178
Size: 30 165 30 995 30 4154
Fragments arranged by size:
4154 995 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5404
BseYI C'CCAG_C
Cuts at: 4607 5103 4607
Size: 496 4908
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 2346 2378 1163
Size: 573 585 25 32 4189
Fragments arranged by size:
4189 585 573 32 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 3615 4643 1540
Size: 275 186 853 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2492 3465 3550 4621 2492
Size: 973 85 1071 3275
Fragments arranged by size:
3275 1071 973 85
BsmI GAATG_Cn'
Cuts at: 2915 3008 2915
Size: 93 5311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2492 2723 3465 3550 4621 1391
Size: 399 702 231 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 399 231 85
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 3293 5023 3293
Size: 1730 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 2432 2687 2759 844
Size: 1508 80 255 72 3489
Fragments arranged by size:
3489 1508 255 80 72
BsrBI CCG'CTC
Cuts at: 1394 1823 3291 1394
Size: 429 1468 3507
Fragments arranged by size:
3507 1468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3899 4073 65
Size: 1183 2651 174 1396
Fragments arranged by size:
2651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 4235 1700
Size: 110 2238 187 2869
Fragments arranged by size:
2869 2238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5404
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5404
BssSI C'ACGA_G
Cuts at: 3464 4476 3464
Size: 1012 4392
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5404
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5404
BstXI CCAn_nnnn'nTGG
Cuts at: 2638 2638
Size: 5404
BstYI r'GATC_y
Cuts at: 1972 2734 3101 3486 3503 4955 5041 5053 5399
Size: 762 367 385 17 1452 86 12 346
Cuts at: 5399 1972
Size: 1977
Fragments arranged by size:
1977 1452 762 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4665
Fragments arranged by size:
4665 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 2920 3714 3734 2390
Size: 530 794 20 4060
Fragments arranged by size:
4060 794 530 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2250 2259
Size: 54 1425 50 275 18 177 243 9
Cuts at: 2259 2439 2851 3740 5369 8
Size: 180 412 889 1629 43
Fragments arranged by size:
1629 1425 889 412 275 243 180 177
54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2851 1537
Size: 275 1039 4090
Fragments arranged by size:
4090 1039 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 3334 1353
Size: 737 1244 3423
Fragments arranged by size:
3423 1244 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3991 4365 4511 387
Size: 1064 138 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2400 2579 3467 4064 4851 5119 1239
Size: 393 768 179 888 597 787 268 1524
Fragments arranged by size:
1524 888 787 768 597 393 268 179
EcoRI G'AATT_C
Cuts at: 2713 3091 2713
Size: 378 5026
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 4551 1678
Size: 2873 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 4045 4077 4119
Size: 32 135 32 826 32 1615 32 42
Cuts at: 4119 4151 1373
Size: 32 2626
Fragments arranged by size:
2626 1615 826 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2748 2994 669
Size: 988 281 810 246 3079
Fragments arranged by size:
3079 988 810 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KpnI G_GTAC'C
Cuts at: 2424 2729 2424
Size: 305 5099
MfeI C'AATT_G
Cuts at: 3003 3003
Size: 5404
MluI A'CGCG_T
Cuts at: 5280 5280
Size: 5404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4518 4702 1305
Size: 3213 184 2007
Fragments arranged by size:
3213 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2252 2261 2441 10
Size: 54 1425 343 177 243 9 180 2973
Fragments arranged by size:
2973 1425 343 243 180 177 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2408 3362 3721 3880 518
Size: 648 1011 231 954 359 159 2042
Fragments arranged by size:
2042 1011 954 648 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 2820 3497 4645 4890 1606
Size: 1214 677 1148 245 2120
Fragments arranged by size:
2120 1214 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
NdeI CA'TA_TG
Cuts at: 387 2321 387
Size: 1934 3470
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2851 2851
Size: 5404
NsiI A_TGCA'T
Cuts at: 2621 2621
Size: 5404
NspI r_CATG'y
Cuts at: 1132 1229 2623 2662 2762 4307 1132
Size: 97 1394 39 100 1545 2229
Fragments arranged by size:
2229 1545 1394 100 97 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5404
PciI A'CATG_T
Cuts at: 4303 4303
Size: 5404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5404
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5404
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PmlI CAC'GTG
Cuts at: 2487 2487
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 4986 5018 3473
Size: 32 1481 32 3859
Fragments arranged by size:
3859 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5404
PsiI TTA'TAA
Cuts at: 2974 2974
Size: 5404
PstI C_TGCA'G
Cuts at: 830 2429 2573 2756 830
Size: 1599 144 183 3478
Fragments arranged by size:
3478 1599 183 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SbfI CC_TGCA'GG
Cuts at: 2429 2756 2429
Size: 327 5077
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2425 2569 2752 3887 4568 4759 826
Size: 219 1380 144 183 1135 681 191 1471
Fragments arranged by size:
1471 1380 1135 681 219 191 183 144
SfiI GGCCn_nnn'nGGCC
Cuts at: 2258 2258
Size: 5404
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2731 1872
Size: 859 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3508 4409 4671 820
Size: 197 926 1565 901 262 1553
Fragments arranged by size:
1565 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5404
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 3328 5
Size: 47 3276 2081
Fragments arranged by size:
3276 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5404
StyI C'CwwG_G
Cuts at: 513 1065 2004 2843 513
Size: 552 939 839 3074
Fragments arranged by size:
3074 939 839 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3431 3448 3601 3786 4231 325
Size: 1255 1851 17 153 185 445 1498
Fragments arranged by size:
1851 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3650 5362
Size: 274 80 33 51 1443 204 1469 1712
Cuts at: 5362 96
Size: 138
Fragments arranged by size:
1712 1469 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5364 5396 5364
Size: 32 5372
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5404
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5404
XmaI C'CCGG_G
Cuts at: 1870 2729 1870
Size: 859 4545
XmnI GAAnn'nnTTC
Cuts at: 3533 3533
Size: 5404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfoI SrfI SwaI
XcmI