Restriction map for
pFN21AE0821
(Circular) (Six-base) MAPSORT of: pFN21AE0821.seq Check: 1228 from: 1 to: 5515
pFN21AE0821 5515 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 3578 4962 5230 1632
Size: 914 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1032 914 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 2267 1166
Size: 1101 4414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 2196
Size: 32 5483
AlwNI CAG_nnn'CTG
Cuts at: 3258 4830 3258
Size: 1572 3943
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2840 1870
Size: 73 897 4545
Fragments arranged by size:
4545 897 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2836 4291 618
Size: 325 217 627 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2182 2834 1391
Size: 791 652 4072
Fragments arranged by size:
4072 791 652
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2009 2212 928
Size: 971 110 203 4231
Fragments arranged by size:
4231 971 203 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2363 2597 4126
Size: 107 122 71 631 605 691 234 1529
Cuts at: 4126 136
Size: 1525
Fragments arranged by size:
1529 1525 691 631 605 234 122 107
71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 3576 4732 1790
Size: 1786 1156 2573
Fragments arranged by size:
2573 1786 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3246 4204 442
Size: 416 53 83 399 24 1829 958 1753
Fragments arranged by size:
1829 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2797 2797
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2687 4175 1239
Size: 890 558 1488 2579
Fragments arranged by size:
2579 1488 890 558
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 3640 4505 4803 2446
Size: 1194 865 298 3158
Fragments arranged by size:
3158 1194 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3322 3704 4339 275
Size: 53 83 186 936 1789 382 635 1451
Fragments arranged by size:
1789 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2196 2226 1178
Size: 30 165 30 793 30 4467
Fragments arranged by size:
4467 793 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2773 1186
Size: 1587 3928
BseYI C'CCAG_C
Cuts at: 2303 2736 4718 5214 2303
Size: 433 1982 496 2604
Fragments arranged by size:
2604 1982 496 433
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4942
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2580 3576 3661 4732 2580
Size: 996 85 1071 3363
Fragments arranged by size:
3363 1071 996 85
BsmI GAATG_Cn'
Cuts at: 2672 3026 3119 2672
Size: 354 93 5068
Fragments arranged by size:
5068 354 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2182 2580 2834 3576 3661 4732 1391
Size: 399 392 398 254 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 392 254 85
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4159 4346 1700
Size: 110 2349 187 2869
Fragments arranged by size:
2869 2349 187 110
BsrGI T'GTAC_A
Cuts at: 96 2151 96
Size: 2055 3460
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2274 2274
Size: 5515
BtgI C'CryG_G
Cuts at: 513 1065 2388 513
Size: 552 1323 3640
Fragments arranged by size:
3640 1323 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 2157 3031 3825 3845 2157
Size: 874 794 20 3827
Fragments arranged by size:
3827 874 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2615 4522 809
Size: 507 1299 1907 1802
Fragments arranged by size:
1907 1802 1299 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 2962 3851
Size: 54 1425 50 275 18 555 577 889
Cuts at: 3851 5480 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 577 555 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2522 2791 3445 1353
Size: 1169 269 654 3423
Fragments arranged by size:
3423 1169 654 269
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2546 2687 3578 4175 4962 5230
Size: 393 497 417 141 891 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 891 787 597 497 417 393 268
141
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
EcoRV GAT'ATC
Cuts at: 2248 2248
Size: 5515
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 4662 1678
Size: 2984 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2169 2201 4156 4188 4230
Size: 32 135 32 597 32 1955 32 42
Cuts at: 4230 4262 1373
Size: 32 2626
Fragments arranged by size:
2626 1955 597 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 2594 3114 2594
Size: 520 4995
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 10
Size: 54 1425 343 555 3138
Fragments arranged by size:
3138 1425 555 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2585 3473 3832 3991 518
Size: 648 1101 318 888 359 159 2042
Fragments arranged by size:
2042 1101 888 648 359 318 159
MspA1I CmG'CkG
Cuts at: 1606 2931 3608 4756 5001 1606
Size: 1325 677 1148 245 2120
Fragments arranged by size:
2120 1325 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2388 513
Size: 552 1323 3640
Fragments arranged by size:
3640 1323 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2047 2873 4418 1132
Size: 97 818 826 1545 2229
Fragments arranged by size:
2229 1545 826 818 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2196 2228 3584 3616 5097 5129 2196
Size: 32 1356 32 1481 32 2582
Fragments arranged by size:
2582 1481 1356 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2164 2867 830
Size: 1334 703 3478
Fragments arranged by size:
3478 1334 703
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2160 2863 3998 4679 4870 826
Size: 219 1115 703 1135 681 191 1471
Fragments arranged by size:
1471 1135 1115 703 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2597 2597
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2425 3619 4520 4782 820
Size: 197 926 482 1194 901 262 1553
Fragments arranged by size:
1553 1194 926 901 482 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 2279 1517
Size: 762 4753
StyI C'CwwG_G
Cuts at: 513 1065 2092 2280 2388 2954 513
Size: 552 1027 188 108 566 3074
Fragments arranged by size:
3074 1027 566 552 188 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3559 3712 3897 4342 325
Size: 1255 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 2417 3761
Size: 274 80 33 51 1443 174 266 1344
Cuts at: 3761 5473 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1344 274 266 174 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2056 2088 5475 5507 2056
Size: 32 3387 32 2064
Fragments arranged by size:
3387 2064 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI XcmI