Restriction map for
pFN21AE0817
(Circular) (Six-base) MAPSORT of: pFN21AE0817.seq Check: 7786 from: 1 to: 5467
pFN21AE0817 5467 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2226 3277 278
Size: 53 83 186 936 690 1051 2468
Fragments arranged by size:
2468 1051 936 690 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2810 1937
Size: 873 4594
Acc65I G'GTAC_C
Cuts at: 2788 2788
Size: 5467
AclI AA'CG_TT
Cuts at: 1563 3594 3967 1563
Size: 2031 373 3063
Fragments arranged by size:
3063 2031 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2047 2365 3530 4914 5182 1632
Size: 415 318 1165 1384 268 1917
Fragments arranged by size:
1917 1384 1165 415 318 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5270
AflIII A'CryG_T
Cuts at: 1203 2418 4366 5343 1203
Size: 1215 1948 977 1327
Fragments arranged by size:
1948 1327 1215 977
AgeI A'CCGG_T
Cuts at: 4298 4298
Size: 5467
AhdI GACnn_n'nnGTC
Cuts at: 4196 4196
Size: 5467
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5467
AlwNI CAG_nnn'CTG
Cuts at: 3210 4782 3210
Size: 1572 3895
ApaLI G'TGCA_C
Cuts at: 3524 4680 3524
Size: 1156 4311
ApoI r'AATT_y
Cuts at: 1493 2776 3002 3154 5263 1493
Size: 1283 226 152 2109 1697
Fragments arranged by size:
2109 1697 1283 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4021 160
Size: 872 2989 1606
Fragments arranged by size:
2989 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
AvaI C'yCGr_G
Cuts at: 1870 1943 2792 1870
Size: 73 849 4545
Fragments arranged by size:
4545 849 73
BamHI G'GATC_C
Cuts at: 2797 3164 2797
Size: 367 5100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2788 4243 618
Size: 325 217 627 1001 1455 1842
Fragments arranged by size:
1842 1455 1001 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2786 1391
Size: 1395 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3658 3692 1903
Size: 34 1721 34 3678
Fragments arranged by size:
3678 1721 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2689 3359 4569 2689
Size: 670 1210 3587
Fragments arranged by size:
3587 1210 670
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4078 136
Size: 107 122 71 631 605 2406 1525
Fragments arranged by size:
2406 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5462 5462
Size: 5467
BlpI GC'TnA_GC
Cuts at: 2884 2884
Size: 5467
Bme1580I G_kGCm'C
Cuts at: 1790 3528 4684 1790
Size: 1738 1156 2573
Fragments arranged by size:
2573 1738 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5467
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2305 3198 4156
Size: 416 53 83 399 24 888 893 958
Cuts at: 4156 442
Size: 1753
Fragments arranged by size:
1753 958 893 888 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2686 4127 1239
Size: 1447 1441 2579
Fragments arranged by size:
2579 1447 1441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3592 4457 4755 3592
Size: 865 298 4304
Fragments arranged by size:
4304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4130 882
Size: 617 2631 2219
Fragments arranged by size:
2631 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3258 493
Size: 711 2054 2702
Fragments arranged by size:
2702 2054 711
BsaBI GATnn'nnATC
Cuts at: 2153 2556 5461 2153
Size: 403 2905 2159
Fragments arranged by size:
2905 2159 403
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 2428 3274 3656
Size: 53 83 186 936 690 205 846 382
Cuts at: 3656 4291 275
Size: 635 1451
Fragments arranged by size:
1451 936 846 690 635 382 205 186
83 53
BsaWI w'CCGG_w
Cuts at: 3900 4298 4572 4719 5130 3900
Size: 398 274 147 411 4237
Fragments arranged by size:
4237 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5242
Fragments arranged by size:
5242 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 1186
Size: 895 4572
BseYI C'CCAG_C
Cuts at: 2172 2469 4670 5166 2172
Size: 297 2201 496 2473
Fragments arranged by size:
2473 2201 496 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2419 1163
Size: 573 683 4211
Fragments arranged by size:
4211 683 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2917 3678 4706 1540
Size: 275 186 916 761 1028 2301
Fragments arranged by size:
2301 1028 916 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3528 3613 4684 3528
Size: 85 1071 4311
Fragments arranged by size:
4311 1071 85
BsmI GAATG_Cn'
Cuts at: 2978 3071 2978
Size: 93 5374
BsmBI CGTCTCn'nnnn_
Cuts at: 2230 2230
Size: 5467
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2786 3528 3613 4684 1391
Size: 399 996 742 85 1071 2174
Fragments arranged by size:
2174 1071 996 742 399 85
BspEI T'CCGG_A
Cuts at: 5130 5130
Size: 5467
BspHI T'CATG_A
Cuts at: 3356 5086 3356
Size: 1730 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2288 2822 844
Size: 1444 534 3489
Fragments arranged by size:
3489 1444 534
BsrBI CCG'CTC
Cuts at: 1394 1823 3354 1394
Size: 429 1531 3507
Fragments arranged by size:
3507 1531 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 3962 4136 65
Size: 1183 1284 1430 174 1396
Fragments arranged by size:
1430 1396 1284 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4111 4298 1700
Size: 110 2301 187 2869
Fragments arranged by size:
2869 2301 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5467
BssSI C'ACGA_G
Cuts at: 3527 4539 3527
Size: 1012 4455
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5467
BstBI TT'CG_AA
Cuts at: 4271 4271
Size: 5467
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5467
BstYI r'GATC_y
Cuts at: 1972 2694 2797 3164 3549 3566 5018 5104 5116
Size: 722 103 367 385 17 1452 86 12
Cuts at: 5116 5462 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 722 385 367 346 103 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2256 2364 513
Size: 552 939 252 108 3616
Fragments arranged by size:
3616 939 552 252 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4728
Fragments arranged by size:
4728 683 56
BtsI GCAGTG_nn'
Cuts at: 2983 3777 3797 2983
Size: 794 20 4653
Fragments arranged by size:
4653 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5467
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2224 2259 529
Size: 35 1660 35 3737
Fragments arranged by size:
3737 1660 35 35
DraI TTT'AAA
Cuts at: 2771 2771
Size: 5467
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4474 809
Size: 507 3158 1802
Fragments arranged by size:
3158 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2914 3803 5432
Size: 54 1425 50 275 18 1084 889 1629
Cuts at: 5432 8
Size: 43
Fragments arranged by size:
1629 1425 1084 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2914 1537
Size: 275 1102 4090
Fragments arranged by size:
4090 1102 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2406 3397 1353
Size: 746 307 991 3423
Fragments arranged by size:
3423 991 746 307
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 4054 4428 4574 387
Size: 1064 138 471 1994 374 146 1280
Fragments arranged by size:
1994 1280 1064 471 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2365 2686 3530 4127 4914 5182
Size: 393 415 318 321 844 597 787 268
Cuts at: 5182 1239
Size: 1524
Fragments arranged by size:
1524 844 787 597 415 393 321 318
268
EcoRI G'AATT_C
Cuts at: 2776 3154 2776
Size: 378 5089
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 5467
FspI TGC'GCA
Cuts at: 3973 3973
Size: 5467
HaeII r_GCGC'y
Cuts at: 1678 4614 1678
Size: 2936 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2593 2625 4108 4140 4182
Size: 32 135 32 1021 32 1483 32 42
Cuts at: 4182 4214 1373
Size: 32 2626
Fragments arranged by size:
2626 1483 1021 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2811 3057 669
Size: 988 281 873 246 3079
Fragments arranged by size:
3079 988 873 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5467
HpaI GTT'AAC
Cuts at: 3057 3057
Size: 5467
KpnI G_GTAC'C
Cuts at: 2792 2792
Size: 5467
MfeI C'AATT_G
Cuts at: 2643 3066 2643
Size: 423 5044
MluI A'CGCG_T
Cuts at: 5343 5343
Size: 5467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4581 4765 1305
Size: 3276 184 2007
Fragments arranged by size:
3276 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3645
Fragments arranged by size:
3645 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2034 2144 2186 2748 3425 3784 3943
Size: 648 868 110 42 562 677 359 159
Cuts at: 3943 518
Size: 2042
Fragments arranged by size:
2042 868 677 648 562 359 159 110
42
MspA1I CmG'CkG
Cuts at: 1606 2176 2883 3560 4708 4953 1606
Size: 570 707 677 1148 245 2120
Fragments arranged by size:
2120 1148 707 677 570 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2256 2364 513
Size: 552 939 252 108 3616
Fragments arranged by size:
3616 939 552 252 108
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5467
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5467
NotI GC'GGCC_GC
Cuts at: 2914 2914
Size: 5467
NspI r_CATG'y
Cuts at: 1132 1229 2422 2825 4370 1132
Size: 97 1193 403 1545 2229
Fragments arranged by size:
2229 1545 1193 403 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5467
PciI A'CATG_T
Cuts at: 2418 4366 2418
Size: 1948 3519
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5467
PmeI GTTT'AAAC
Cuts at: 2771 2771
Size: 5467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 5049 5081 3536
Size: 32 1481 32 3922
Fragments arranged by size:
3922 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5467
PsiI TTA'TAA
Cuts at: 3037 3037
Size: 5467
PstI C_TGCA'G
Cuts at: 830 2819 830
Size: 1989 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5467
PvuII CAG'CTG
Cuts at: 2176 2176
Size: 5467
SalI G'TCGA_C
Cuts at: 1936 2809 1936
Size: 873 4594
SbfI CC_TGCA'GG
Cuts at: 2819 2819
Size: 5467
ScaI AGT'ACT
Cuts at: 3715 5427 3715
Size: 1712 3755
SfcI C'TryA_G
Cuts at: 826 1045 2116 2815 3950 4631 4822 826
Size: 219 1071 699 1135 681 191 1471
Fragments arranged by size:
1471 1135 1071 699 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5467
SmaI CCC'GGG
Cuts at: 1872 2794 1872
Size: 922 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3571 4472 4734 820
Size: 197 926 1628 901 262 1553
Fragments arranged by size:
1628 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3258 493
Size: 2765 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5467
SphI G_CATG'C
Cuts at: 1132 2825 1132
Size: 1693 3774
SspI AAT'ATT
Cuts at: 5 52 3391 5
Size: 47 3339 2081
Fragments arranged by size:
3339 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5467
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 2364 2906 513
Size: 552 939 252 108 542 3074
Fragments arranged by size:
3074 939 552 542 252 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2738 3494 3511 3664 3849 4294
Size: 1255 417 741 756 17 153 185 445
Cuts at: 4294 325
Size: 1498
Fragments arranged by size:
1498 1255 756 741 445 417 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3713 5425 96
Size: 274 80 33 51 1443 1736 1712 138
Fragments arranged by size:
1736 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2128 2160 5427 5459 2128
Size: 32 3267 32 2136
Fragments arranged by size:
3267 2136 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5467
XbaI T'CTAG_A
Cuts at: 2803 2803
Size: 5467
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5467
XmaI C'CCGG_G
Cuts at: 1870 2792 1870
Size: 922 4545
XmnI GAAnn'nnTTC
Cuts at: 3596 3596
Size: 5467
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2224 3275 276
Size: 53 83 186 936 690 1051 2468
Fragments arranged by size:
2468 1051 936 690 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI