Restriction map for
pFN21AE0816
(Circular) (Six-base) MAPSORT of: pFN21AE0816.seq Check: 2371 from: 1 to: 5383
pFN21AE0816 5383 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 278
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 2547 2726 1937
Size: 169 441 179 4594
Fragments arranged by size:
4594 441 179 169
Acc65I G'GTAC_C
Cuts at: 2370 2704 2370
Size: 334 5049
AclI AA'CG_TT
Cuts at: 1563 3510 3883 1563
Size: 1947 373 3063
Fragments arranged by size:
3063 1947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3446 4830 5098 1632
Size: 1814 1384 268 1917
Fragments arranged by size:
1917 1814 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5186
AflIII A'CryG_T
Cuts at: 1203 4282 5259 1203
Size: 3079 977 1327
Fragments arranged by size:
3079 1327 977
AgeI A'CCGG_T
Cuts at: 4214 4214
Size: 5383
AhdI GACnn_n'nnGTC
Cuts at: 4112 4112
Size: 5383
AleI CACnn'nnGTG
Cuts at: 1166 2636 1166
Size: 1470 3913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5383
AlwNI CAG_nnn'CTG
Cuts at: 3126 4698 3126
Size: 1572 3811
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2070 2254 2692 2918 3070 5179 1493
Size: 577 184 438 226 152 2109 1697
Fragments arranged by size:
2109 1697 577 438 226 184 152
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 1870
Size: 73 765 4545
Fragments arranged by size:
4545 765 73
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2370 2704 4159 618
Size: 325 217 627 583 334 1455 1842
Fragments arranged by size:
1842 1455 627 583 334 325 217
BanII G_rGCy'C
Cuts at: 1391 2615 2702 1391
Size: 1224 87 4072
Fragments arranged by size:
4072 1224 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3574 3608 1903
Size: 34 1637 34 3678
Fragments arranged by size:
3678 1637 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 2527 1548
Size: 979 4404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3994 136
Size: 107 122 71 631 605 2322 1525
Fragments arranged by size:
2322 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2214 5378 2214
Size: 3164 2219
BlpI GC'TnA_GC
Cuts at: 2800 2800
Size: 5383
Bme1580I G_kGCm'C
Cuts at: 1790 2330 3444 4600 1790
Size: 540 1114 1156 2573
Fragments arranged by size:
2573 1156 1114 540
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2133 3114 4072
Size: 416 53 83 399 24 716 981 958
Cuts at: 4072 442
Size: 1753
Fragments arranged by size:
1753 981 958 716 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4043 1239
Size: 2804 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3508 4373 4671 3508
Size: 865 298 4220
Fragments arranged by size:
4220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4046 882
Size: 617 2547 2219
Fragments arranged by size:
2547 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2185 3174 493
Size: 711 981 989 2702
Fragments arranged by size:
2702 989 981 711
BsaBI GATnn'nnATC
Cuts at: 5377 5377
Size: 5383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3190 3572 4207 275
Size: 53 83 186 936 1657 382 635 1451
Fragments arranged by size:
1657 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3816 4214 4488 4635 5046 3816
Size: 398 274 147 411 4153
Fragments arranged by size:
4153 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2387 2417 1178
Size: 30 165 30 984 30 4144
Fragments arranged by size:
4144 984 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5383
BseYI C'CCAG_C
Cuts at: 4586 5082 4586
Size: 496 4887
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2166 2215 2378 1163
Size: 573 430 49 163 4168
Fragments arranged by size:
4168 573 430 163 49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 3594 4622 1540
Size: 275 186 832 761 1028 2301
Fragments arranged by size:
2301 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2615 2670 3444 3529 4600 2190
Size: 425 55 774 85 1071 2973
Fragments arranged by size:
2973 1071 774 425 85 55
BsmI GAATG_Cn'
Cuts at: 2342 2894 2987 2342
Size: 552 93 4738
Fragments arranged by size:
4738 552 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2330 2615 2670 2702 3444 3529
Size: 399 400 140 285 55 32 742 85
Cuts at: 3529 4600 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 285 140 85
55 32
BspEI T'CCGG_A
Cuts at: 5046 5046
Size: 5383
BspHI T'CATG_A
Cuts at: 3272 5002 3272
Size: 1730 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2237 2738 844
Size: 1393 501 3489
Fragments arranged by size:
3489 1393 501
BsrBI CCG'CTC
Cuts at: 1394 1823 3270 1394
Size: 429 1447 3507
Fragments arranged by size:
3507 1447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3878 4052 65
Size: 1183 2630 174 1396
Fragments arranged by size:
2630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4027 4214 1700
Size: 110 2217 187 2869
Fragments arranged by size:
2869 2217 187 110
BsrGI T'GTAC_A
Cuts at: 96 2640 96
Size: 2544 2839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5383
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 4187 4187
Size: 5383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2426 2248
Size: 178 5205
BstYI r'GATC_y
Cuts at: 1972 2214 2713 3080 3465 3482 4934 5020 5032
Size: 242 499 367 385 17 1452 86 12
Cuts at: 5032 5378 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 499 385 367 346 242 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2634 513
Size: 552 1569 3262
Fragments arranged by size:
3262 1569 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2154 2390 2899 3693 3713 2154
Size: 236 509 794 20 3824
Fragments arranged by size:
3824 794 509 236 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5348
DraI TTT'AAA
Cuts at: 2687 2687
Size: 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2376 2416
Size: 54 1425 50 275 18 303 243 40
Cuts at: 2416 2830 3719 5348 8
Size: 414 889 1629 43
Fragments arranged by size:
1629 1425 889 414 303 275 243 54
50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 2830 1537
Size: 275 1018 4090
Fragments arranged by size:
4090 1018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3313 1353
Size: 1960 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3970 4344 4490 387
Size: 1064 138 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2613 2613
Size: 5383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3446 4043 4830 5098 1239
Size: 393 1814 597 787 268 1524
Fragments arranged by size:
1814 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2652 2652
Size: 5383
EcoRI G'AATT_C
Cuts at: 2692 3070 2692
Size: 378 5005
EcoRV GAT'ATC
Cuts at: 2022 2022
Size: 5383
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 4530 1678
Size: 2852 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4024 4056 4098 4130 1373
Size: 32 135 32 2452 32 42 32 2626
Fragments arranged by size:
2626 2452 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2727 2973 669
Size: 988 281 789 246 3079
Fragments arranged by size:
3079 988 789 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5383
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KpnI G_GTAC'C
Cuts at: 2374 2708 2374
Size: 334 5049
MfeI C'AATT_G
Cuts at: 2982 2982
Size: 5383
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4497 4681 1305
Size: 3192 184 2007
Fragments arranged by size:
3192 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2378 2418 10
Size: 54 1425 343 303 243 40 2975
Fragments arranged by size:
2975 1425 343 303 243 54 40
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 2636 3341 3700 3859 518
Size: 648 1269 201 705 359 159 2042
Fragments arranged by size:
2042 1269 705 648 359 201 159
MspA1I CmG'CkG
Cuts at: 1606 2799 3476 4624 4869 1606
Size: 1193 677 1148 245 2120
Fragments arranged by size:
2120 1193 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2634 513
Size: 552 1569 3262
Fragments arranged by size:
3262 1569 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2248 2597 2741 4286 1132
Size: 97 1019 349 144 1545 2229
Fragments arranged by size:
2229 1545 1019 349 144 97
PasI CC'CwG_GG
Cuts at: 1348 2323 1348
Size: 975 4408
PciI A'CATG_T
Cuts at: 4282 4282
Size: 5383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5383
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 4965 4997 3452
Size: 32 1481 32 3838
Fragments arranged by size:
3838 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2652 2652
Size: 5383
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2953 2953
Size: 5383
PstI C_TGCA'G
Cuts at: 830 2735 830
Size: 1905 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
SacI G_AGCT'C
Cuts at: 2615 2615
Size: 5383
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SbfI CC_TGCA'GG
Cuts at: 2735 2735
Size: 5383
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SfcI C'TryA_G
Cuts at: 826 1045 2731 3866 4547 4738 826
Size: 219 1686 1135 681 191 1471
Fragments arranged by size:
1686 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5383
SmaI CCC'GGG
Cuts at: 1872 2710 1872
Size: 838 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3487 4388 4650 820
Size: 197 926 1544 901 262 1553
Fragments arranged by size:
1553 1544 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SspI AAT'ATT
Cuts at: 5 52 3307 5
Size: 47 3255 2081
Fragments arranged by size:
3255 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5383
StyI C'CwwG_G
Cuts at: 513 1065 2464 2634 2647 2661 2822 513
Size: 552 1399 170 13 14 161 3074
Fragments arranged by size:
3074 1399 552 170 161 14 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2403 3410 3427 3580 3765 4210 325
Size: 1255 823 1007 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1007 823 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2640 3629 5341
Size: 274 80 33 51 1443 663 989 1712
Cuts at: 5341 96
Size: 138
Fragments arranged by size:
1712 1443 989 663 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 5343 5375 2387
Size: 32 2924 32 2395
Fragments arranged by size:
2924 2395 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5383
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5383
XmaI C'CCGG_G
Cuts at: 1870 2708 1870
Size: 838 4545
XmnI GAAnn'nnTTC
Cuts at: 3512 3512
Size: 5383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 276
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI