Restriction map for
pFN21AE0811
(Circular) (Six-base) MAPSORT of: pFN21AE0811.seq Check: 401 from: 1 to: 5830
pFN21AE0811 5830 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2604 3173 1937
Size: 667 569 4594
Fragments arranged by size:
4594 667 569
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3034 3893 5277 5545 1632
Size: 1402 859 1384 268 1917
Fragments arranged by size:
1917 1402 1384 859 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 3573 5145 3573
Size: 1572 4258
ApaI G_GGCC'C
Cuts at: 2754 2901 3058 2754
Size: 147 157 5526
Fragments arranged by size:
5526 157 147
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 2828 3139 3365 3517 5626 1493
Size: 1335 311 226 152 2109 1697
Fragments arranged by size:
2109 1697 1335 311 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 2698 3155 1870
Size: 73 755 457 4545
Fragments arranged by size:
4545 755 457 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 2540 2588 2662 2702
Size: 325 217 627 479 274 48 74 40
Cuts at: 2702 2942 3151 4606 618
Size: 240 209 1455 1842
Fragments arranged by size:
1842 1455 627 479 325 274 240 217
209 74 48 40
BanII G_rGCy'C
Cuts at: 1391 2754 2901 3058 3080 3149 1391
Size: 1363 147 157 22 69 4072
Fragments arranged by size:
4072 1363 157 147 69 22
BbeI G_GCGC'C
Cuts at: 2270 2544 2592 2706 2946 2270
Size: 274 48 114 240 5154
Fragments arranged by size:
5154 274 240 114 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BbvCI CC'TCA_GC
Cuts at: 3038 3038
Size: 5830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2278 2312 4021 4055 1903
Size: 34 341 34 1709 34 3678
Fragments arranged by size:
3678 1709 341 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2106 2306 2409
Size: 107 122 71 631 605 434 200 103
Cuts at: 2409 2447 2788 4441 136
Size: 38 341 1653 1525
Fragments arranged by size:
1653 1525 631 605 434 341 200 122
107 103 71 38
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 2078 3247 2078
Size: 1169 4661
Bme1580I G_kGCm'C
Cuts at: 1790 2667 2754 2901 3058 3891 5047 1790
Size: 877 87 147 157 833 1156 2573
Fragments arranged by size:
2573 1156 877 833 157 147 87
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3052 3561 4519
Size: 416 53 83 399 24 1635 509 958
Cuts at: 4519 442
Size: 1753
Fragments arranged by size:
1753 1635 958 509 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2543 4490 1239
Size: 1304 1947 2579
Fragments arranged by size:
2579 1947 1304
Bpu10I CC'TnA_GC
Cuts at: 3038 3038
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 3955 4820 5118 2048
Size: 1907 865 298 2760
Fragments arranged by size:
2760 1907 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4493 882
Size: 617 2994 2219
Fragments arranged by size:
2994 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2267 2355 2541 2589
Size: 53 83 186 936 734 88 186 48
Cuts at: 2589 2703 2943 3637 4019 4654 275
Size: 114 240 694 382 635 1451
Fragments arranged by size:
1451 936 734 694 635 382 240 186
186 114 88 83 53 48
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5605
Fragments arranged by size:
5605 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 2879 1186
Size: 1618 75 4137
Fragments arranged by size:
4137 1618 75
BseYI C'CCAG_C
Cuts at: 2281 2344 2554 5033 5529 2281
Size: 63 210 2479 496 2582
Fragments arranged by size:
2582 2479 496 210 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 1163
Size: 573 399 4858
Fragments arranged by size:
4858 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2226 2256 2635 2721 2746
Size: 275 186 51 174 30 379 86 25
Cuts at: 2746 3280 4041 5069 1540
Size: 534 761 1028 2301
Fragments arranged by size:
2301 1028 761 534 379 275 186 174
86 51 30 25
BsiHKAI G_wGCw'C
Cuts at: 3891 3976 5047 3891
Size: 85 1071 4674
Fragments arranged by size:
4674 1071 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2667 2754 2901 3058 3080 3149 3891
Size: 399 877 87 147 157 22 69 742
Cuts at: 3891 3976 5047 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 877 742 399 157 147 87
85 69 22
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2928 3185 844
Size: 1334 750 257 3489
Fragments arranged by size:
3489 1334 750 257
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2408 2708 2996 4474 4661 1700
Size: 110 598 300 288 1478 187 2869
Fragments arranged by size:
2869 1478 598 300 288 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 2203 2205 2277 2550 2654 2802 2804 1925
Size: 278 2 72 273 104 148 2 4951
Fragments arranged by size:
4951 278 273 148 104 72 2 2
BssSI C'ACGA_G
Cuts at: 2816 3890 4902 2816
Size: 1074 1012 3744
Fragments arranged by size:
3744 1074 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5830
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825
Size: 1188 367 385 17 1452 86 12 346
Cuts at: 5825 1972
Size: 1977
Fragments arranged by size:
1977 1452 1188 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2256 2463 2577 2692 2746 2797
Size: 552 939 252 207 114 115 54 51
Cuts at: 2797 513
Size: 3546
Fragments arranged by size:
3546 939 552 252 207 115 114 54
51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2359 487
Size: 683 56 1133 3958
Fragments arranged by size:
3958 1133 683 56
BtsI GCAGTG_nn'
Cuts at: 3346 4140 4160 3346
Size: 794 20 5016
Fragments arranged by size:
5016 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2070 2190
Size: 54 1425 50 275 18 219 21 120
Cuts at: 2190 2223 2253 2307 2370 2406 2414 2460 2574
Size: 33 30 54 63 36 8 46 114
Cuts at: 2574 2710 2718 2743 2910 3277 4166 5795 8
Size: 136 8 25 167 367 889 1629 43
Fragments arranged by size:
1629 1425 889 367 275 219 167 136
120 114 63 54 54 50 46 43
36 33 30 25 21 18 8 8
EagI C'GGCC_G
Cuts at: 1537 1812 2049 2223 2253 2718 2743 3277 1537
Size: 275 237 174 30 465 25 534 4090
Fragments arranged by size:
4090 534 465 275 237 174 30 25
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 2624 2837 2885 3029 3760 1353
Size: 968 303 213 48 144 731 3423
Fragments arranged by size:
3423 968 731 303 213 144 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2600 4417 4791 4937 387
Size: 1064 138 1011 1817 374 146 1280
Fragments arranged by size:
1817 1280 1064 1011 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2543 3034 3893 4490 5277 5545 1239
Size: 393 911 491 859 597 787 268 1524
Fragments arranged by size:
1524 911 859 787 597 491 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2897 2898 2926 3054 2897
Size: 1 28 128 5673
Fragments arranged by size:
5673 128 28 1
EcoRI G'AATT_C
Cuts at: 2828 3139 3517 2828
Size: 311 378 5141
Fragments arranged by size:
5141 378 311
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 2270 2404 2453 2459 2544 2592 2706 2946
Size: 592 134 49 6 85 48 114 240
Cuts at: 2946 4977 1678
Size: 2031 2531
Fragments arranged by size:
2531 2031 592 240 134 114 85 49
48 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373
Size: 32 135 32 2899 32 42 32 2626
Fragments arranged by size:
2899 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KasI G'GCGC_C
Cuts at: 2266 2540 2588 2702 2942 2266
Size: 274 48 114 240 5154
Fragments arranged by size:
5154 274 240 114 48
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 2372 2912 10
Size: 54 1425 343 240 300 540 2928
Fragments arranged by size:
2928 1425 540 343 300 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 3788 4147 4306 518
Size: 648 1266 1356 359 159 2042
Fragments arranged by size:
2042 1356 1266 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2258 2465 2530 2748 2799 3246 3923
Size: 428 224 207 65 218 51 447 677
Cuts at: 3923 5071 5316 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 447 428 245 224 218
207 65 51
NaeI GCC'GGC
Cuts at: 2410 2998 2410
Size: 588 5242
NarI GG'CG_CC
Cuts at: 2267 2541 2589 2703 2943 2267
Size: 274 48 114 240 5154
Fragments arranged by size:
5154 274 240 114 48
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4339
Fragments arranged by size:
4339 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NgoMIV G'CCGG_C
Cuts at: 2408 2996 2408
Size: 588 5242
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 2049 2253 2718 3277 2049
Size: 204 465 559 4602
Fragments arranged by size:
4602 559 465 204
NspI r_CATG'y
Cuts at: 1132 1229 3188 4733 1132
Size: 97 1959 1545 2229
Fragments arranged by size:
2229 1959 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2056 1348
Size: 708 5122
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2926 2926
Size: 5830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspOMI G'GGCC_C
Cuts at: 2750 2897 3054 2750
Size: 147 157 5526
Fragments arranged by size:
5526 157 147
PstI C_TGCA'G
Cuts at: 830 3182 830
Size: 2352 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SacII CC_GC'GG
Cuts at: 2259 2466 2749 2800 2259
Size: 207 283 51 5289
Fragments arranged by size:
5289 283 207 51
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2321 2624 2885 2321
Size: 303 261 5266
Fragments arranged by size:
5266 303 261
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 3178 4313 4994 5185 826
Size: 219 2133 1135 681 191 1471
Fragments arranged by size:
2133 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2788 2788
Size: 5830
SfoI GGC'GCC
Cuts at: 2268 2542 2590 2704 2944 2268
Size: 274 48 114 240 5154
Fragments arranged by size:
5154 274 240 114 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 2700 3157 1872
Size: 828 457 4545
Fragments arranged by size:
4545 828 457
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 3934 4835 5097 820
Size: 197 926 120 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197 120
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SrfI GCCC'GGGC
Cuts at: 2700 2700
Size: 5830
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 2957 1517
Size: 1440 4390
StyI C'CwwG_G
Cuts at: 513 1065 2004 3269 513
Size: 552 939 1265 3074
Fragments arranged by size:
3074 1265 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3104 3857 3874 4027 4212 4657 325
Size: 1255 1524 753 17 153 185 445 1498
Fragments arranged by size:
1524 1498 1255 753 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4076 5788 96
Size: 274 80 33 51 1443 2099 1712 138
Fragments arranged by size:
2099 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2498 2530 5790 5822 2498
Size: 32 3260 32 2506
Fragments arranged by size:
3260 2506 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 2698 3155 1870
Size: 828 457 4545
Fragments arranged by size:
4545 828 457
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SanDI SexAI
SwaI XcmI