Restriction map for
pFN21AE0810
(Circular) (Six-base) MAPSORT of: pFN21AE0810.seq Check: 6774 from: 1 to: 5620
pFN21AE0810 5620 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2446 2941 2446
Size: 495 5125
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3683 5067 5335 1632
Size: 2051 1384 268 1917
Fragments arranged by size:
2051 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 2298 4519 5496 1203
Size: 1095 2221 977 1327
Fragments arranged by size:
2221 1327 1095 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 2534 1166
Size: 1368 4252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2787 2848 1184
Size: 1603 61 3956
Fragments arranged by size:
3956 1603 61
AlwNI CAG_nnn'CTG
Cuts at: 3363 4935 3363
Size: 1572 4048
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2029 2270 2378 2507 2929 3155 3307 5416
Size: 536 241 108 129 422 226 152 2109
Cuts at: 5416 1493
Size: 1697
Fragments arranged by size:
2109 1697 536 422 241 226 152 129
108
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2446 2941 4396 618
Size: 325 217 627 659 495 1455 1842
Fragments arranged by size:
1842 1455 659 627 495 325 217
BanII G_rGCy'C
Cuts at: 1391 2674 2939 1391
Size: 1283 265 4072
Fragments arranged by size:
4072 1283 265
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 2590 2599 928
Size: 971 347 344 9 3949
Fragments arranged by size:
3949 971 347 344 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3811 3845 1903
Size: 34 50 34 1790 34 3678
Fragments arranged by size:
3678 1790 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 2673 3037 2673
Size: 364 5256
Bme1580I G_kGCm'C
Cuts at: 1790 3681 4837 1790
Size: 1891 1156 2573
Fragments arranged by size:
2573 1891 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2008 4280 1239
Size: 769 2272 2579
Fragments arranged by size:
2579 2272 769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2833 3745 4610 4908 2833
Size: 912 865 298 3545
Fragments arranged by size:
3545 912 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 1178
Size: 30 165 30 603 30 4762
Fragments arranged by size:
4762 603 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2507 1186
Size: 1321 4299
BseYI C'CCAG_C
Cuts at: 4823 5319 4823
Size: 496 5124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2273 2648 1163
Size: 573 537 375 4135
Fragments arranged by size:
4135 573 537 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 3681 3766 4837 2499
Size: 1182 85 1071 3282
Fragments arranged by size:
3282 1182 1071 85
BsmI GAATG_Cn'
Cuts at: 2764 3131 3224 2764
Size: 367 93 5160
Fragments arranged by size:
5160 367 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2499 2674 2939 3681 3766 4837 1391
Size: 399 709 175 265 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 709 399 265 175 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 2335 1184
Size: 1151 4469
BstYI r'GATC_y
Cuts at: 1972 2739 2950 3317 3702 3719 5171 5257 5269
Size: 767 211 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 767 385 367 346 211 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2160 513
Size: 552 1095 3973
Fragments arranged by size:
3973 1095 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2506 2924 2506
Size: 418 5202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 3067 3956
Size: 54 1425 50 275 18 181 1056 889
Cuts at: 3956 5585 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1056 889 275 181 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3550 1353
Size: 2197 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 3683 4280 5067 5335 1239
Size: 393 376 1675 597 787 268 1524
Fragments arranged by size:
1675 1524 787 597 393 376 268
EcoRI G'AATT_C
Cuts at: 2029 2378 2929 3307 2029
Size: 349 551 378 4342
Fragments arranged by size:
4342 551 378 349
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2334 2366 4261 4293 4335
Size: 32 135 32 762 32 1895 32 42
Cuts at: 4335 4367 1373
Size: 32 2626
Fragments arranged by size:
2626 1895 762 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2450 2945 2450
Size: 495 5125
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2013 10
Size: 54 1425 343 181 3617
Fragments arranged by size:
3617 1425 343 181 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2534 2615 2769 3578 3937 4096
Size: 648 1131 237 81 154 809 359 159
Cuts at: 4096 518
Size: 2042
Fragments arranged by size:
2042 1131 809 648 359 237 159 154
81
MspA1I CmG'CkG
Cuts at: 1606 2677 3036 3713 4861 5106 1606
Size: 1071 359 677 1148 245 2120
Fragments arranged by size:
2120 1148 1071 677 359 245
NcoI C'CATG_G
Cuts at: 513 1065 2160 513
Size: 552 1095 3973
Fragments arranged by size:
3973 1095 552
NdeI CA'TA_TG
Cuts at: 387 2533 387
Size: 2146 3474
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2302 2399 2422 2978 4523 1132
Size: 97 1073 97 23 556 1545 2229
Fragments arranged by size:
2229 1545 1073 556 97 97 23
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 2298 4519 2298
Size: 2221 3399
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PfoI T'CCnGG_A
Cuts at: 2024 2024
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 2799 3190 2799
Size: 391 5229
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2294 2326 2294
Size: 32 5588
PstI C_TGCA'G
Cuts at: 830 2972 830
Size: 2142 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
PvuII CAG'CTG
Cuts at: 2677 2677
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2046 2383 2968 4103 4784 4975 826
Size: 219 1001 337 585 1135 681 191 1471
Fragments arranged by size:
1471 1135 1001 681 585 337 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2812 3724 4625 4887 820
Size: 197 926 869 912 901 262 1553
Fragments arranged by size:
1553 926 912 901 869 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2399 2978 1132
Size: 1267 579 3774
Fragments arranged by size:
3774 1267 579
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2160 3059 513
Size: 552 1095 899 3074
Fragments arranged by size:
3074 1095 899 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2093 3647 3664 3817 4002 4447 325
Size: 1255 513 1554 17 153 185 445 1498
Fragments arranged by size:
1554 1498 1255 513 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 5580 5612 2006
Size: 32 3542 32 2014
Fragments arranged by size:
3542 2014 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI