Restriction map for
pFN21AE0806
(Circular) (Six-base) MAPSORT of: pFN21AE0806.seq Check: 9455 from: 1 to: 5794
pFN21AE0806 5794 bp
With 182 enzymes: SgfI *
March 28, 2012 12:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 3137 1937
Size: 436 764 4594
Fragments arranged by size:
4594 764 436
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 2527 3857 5241 5509 1632
Size: 881 14 1330 1384 268 1917
Fragments arranged by size:
1917 1384 1330 881 268 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 4693 5670 1203
Size: 3490 977 1327
Fragments arranged by size:
3490 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 2363 1166
Size: 1197 4597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2548 1184
Size: 1364 4430
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 2813 2845 2405
Size: 32 376 32 5354
Fragments arranged by size:
5354 376 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2219 2550 3537 5109 2219
Size: 331 987 1572 2904
Fragments arranged by size:
2904 1572 987 331
ApaI G_GGCC'C
Cuts at: 3083 3083
Size: 5794
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 2527 3103 3329 3481 5590 1493
Size: 1034 576 226 152 2109 1697
Fragments arranged by size:
2109 1697 1034 576 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 1870
Size: 73 1176 4545
Fragments arranged by size:
4545 1176 73
BamHI G'GATC_C
Cuts at: 2757 3124 3491 2757
Size: 367 367 5060
Fragments arranged by size:
5060 367 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 3115 4570 618
Size: 325 217 627 226 1102 1455 1842
Fragments arranged by size:
1842 1455 1102 627 325 226 217
BanII G_rGCy'C
Cuts at: 1391 2997 3068 3083 3113 1391
Size: 1606 71 15 30 4072
Fragments arranged by size:
4072 1606 71 30 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2971 3005 3985 4019 1903
Size: 34 1034 34 980 34 3678
Fragments arranged by size:
3678 1034 980 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 2232 2928 1548
Size: 684 696 4414
Fragments arranged by size:
4414 696 684
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4405 136
Size: 107 122 71 631 605 2733 1525
Fragments arranged by size:
2733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2418 5789 2418
Size: 3371 2423
BlpI GC'TnA_GC
Cuts at: 2626 3211 2626
Size: 585 5209
Bme1580I G_kGCm'C
Cuts at: 1790 2792 3083 3855 5011 1790
Size: 1002 291 772 1156 2573
Fragments arranged by size:
2573 1156 1002 772 291
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3525 4483 442
Size: 416 53 83 399 24 2108 958 1753
Fragments arranged by size:
2108 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2609 2662 2878 2927 3012 4454 1239
Size: 893 477 53 216 49 85 1442 2579
Fragments arranged by size:
2579 1442 893 477 216 85 53 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 3919 4784 5082 2156
Size: 1763 865 298 2868
Fragments arranged by size:
2868 1763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4457 882
Size: 617 2958 2219
Fragments arranged by size:
2958 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3585 493
Size: 711 2381 2702
Fragments arranged by size:
2702 2381 711
BsaBI GATnn'nnATC
Cuts at: 2507 2762 5788 2507
Size: 255 3026 2513
Fragments arranged by size:
3026 2513 255
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3601 3983 4618 275
Size: 53 83 186 936 2068 382 635 1451
Fragments arranged by size:
2068 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3050 4227 4625 4899 5046 5457 3050
Size: 1177 398 274 147 411 3387
Fragments arranged by size:
3387 1177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 2876 2903 2906
Size: 30 165 30 1002 30 441 27 3
Cuts at: 2906 2933 1178
Size: 27 4039
Fragments arranged by size:
4039 1002 441 165 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5794
BseYI C'CCAG_C
Cuts at: 2741 4997 5493 2741
Size: 2256 496 3042
Fragments arranged by size:
3042 2256 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2554 2762 1163
Size: 573 818 208 4195
Fragments arranged by size:
4195 818 573 208
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3244 4005 5033 1540
Size: 275 186 1243 761 1028 2301
Fragments arranged by size:
2301 1243 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2997 3855 3940 5011 2997
Size: 858 85 1071 3780
Fragments arranged by size:
3780 1071 858 85
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2792 2997 3068 3083 3113 3855 3940
Size: 399 1002 205 71 15 30 742 85
Cuts at: 3940 5011 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1002 742 399 205 85 71
30 15
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 3683 5413 3683
Size: 1730 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3149 844
Size: 2305 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2814 3681 1394
Size: 429 991 867 3507
Fragments arranged by size:
3507 991 867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2143 4289 4463 65
Size: 1183 895 2146 174 1396
Fragments arranged by size:
2146 1396 1183 895 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2098 2304 2667 4438 4625 1700
Size: 110 288 206 363 1771 187 2869
Fragments arranged by size:
2869 1771 363 288 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 2983 3854 4866 2983
Size: 871 1012 3911
Fragments arranged by size:
3911 1012 871
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2020 794
Size: 1226 4568
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstYI r'GATC_y
Cuts at: 1972 2418 2757 3124 3491 3876 3893 5345 5431
Size: 446 339 367 367 385 17 1452 86
Cuts at: 5431 5443 5789 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 446 385 367 367 346 339
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2877 3059 513
Size: 552 1812 182 3248
Fragments arranged by size:
3248 1812 552 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 3310 4104 4124 2220
Size: 1090 794 20 3890
Fragments arranged by size:
3890 1090 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2081 4801 809
Size: 507 765 2720 1802
Fragments arranged by size:
2720 1802 765 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2712 3042 3241
Size: 54 1425 50 275 18 882 330 199
Cuts at: 3241 4130 5759 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 882 330 275 199 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3241 1537
Size: 275 1429 4090
Fragments arranged by size:
4090 1429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 3724 1353
Size: 968 1403 3423
Fragments arranged by size:
3423 1403 968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4381 4755 4901 387
Size: 1064 138 2792 374 146 1280
Fragments arranged by size:
2792 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2995 2995
Size: 5794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2513 2527 2609 2662 2878 2927
Size: 393 500 381 14 82 53 216 49
Cuts at: 2927 3012 3857 4454 5241 5509 1239
Size: 85 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 597 500 393 381 268
216 85 82 53 49 14
EcoO109I rG'GnC_Cy
Cuts at: 3004 3004
Size: 5794
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 3027 4941 1678
Size: 1349 1914 2531
Fragments arranged by size:
2531 1914 1349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 4435 4467 4509
Size: 32 135 32 838 32 1993 32 42
Cuts at: 4509 4541 1373
Size: 32 2626
Fragments arranged by size:
2626 1993 838 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3138 3384 669
Size: 988 281 1200 246 3079
Fragments arranged by size:
3079 1200 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4908 5092 1305
Size: 3603 184 2007
Fragments arranged by size:
3603 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2714 3044 10
Size: 54 1425 343 882 330 2760
Fragments arranged by size:
2760 1425 882 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2705 2708 2855 3752 4111 4270
Size: 648 1197 342 3 147 897 359 159
Cuts at: 4270 518
Size: 2042
Fragments arranged by size:
2042 1197 897 648 359 342 159 147
3
MspA1I CmG'CkG
Cuts at: 1606 2937 3210 3887 5035 5280 1606
Size: 1331 273 677 1148 245 2120
Fragments arranged by size:
2120 1331 1148 677 273 245
NaeI GCC'GGC
Cuts at: 2306 2306
Size: 5794
NcoI C'CATG_G
Cuts at: 513 1065 3059 513
Size: 552 1994 3248
Fragments arranged by size:
3248 1994 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NgoMIV G'CCGG_C
Cuts at: 2304 2304
Size: 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 2675 2869 3152 4697 1132
Size: 97 1446 194 283 1545 2229
Fragments arranged by size:
2229 1545 1446 283 194 97
PasI CC'CwG_GG
Cuts at: 1348 2605 1348
Size: 1257 4537
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5794
PfoI T'CCnGG_A
Cuts at: 2177 2586 2177
Size: 409 5385
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2405 2437 3863 3895 5376 5408 2405
Size: 32 1426 32 1481 32 2791
Fragments arranged by size:
2791 1481 1426 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3004 3004
Size: 5794
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PspOMI G'GGCC_C
Cuts at: 3079 3079
Size: 5794
PstI C_TGCA'G
Cuts at: 830 2025 3146 830
Size: 1195 1121 3478
Fragments arranged by size:
3478 1195 1121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
SacI G_AGCT'C
Cuts at: 2997 2997
Size: 5794
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SanDI GG'GwC_CC
Cuts at: 3004 3004
Size: 5794
SapI GCTCTTCn'nnn_
Cuts at: 2321 2321
Size: 5794
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 2021 3142 4277 4958 5149 826
Size: 219 976 1121 1135 681 191 1471
Fragments arranged by size:
1471 1135 1121 976 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 2098 1700
Size: 398 5396
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 3898 4799 5061 820
Size: 197 926 228 1727 901 262 1553
Fragments arranged by size:
1727 1553 926 901 262 228 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 2675 2869 3152 1132
Size: 1543 194 283 3774
Fragments arranged by size:
3774 1543 283 194
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5794
StyI C'CwwG_G
Cuts at: 513 1065 3059 3233 513
Size: 552 1994 174 3074
Fragments arranged by size:
3074 1994 552 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3821 3838 3991 4176 4621 325
Size: 1255 2241 17 153 185 445 1498
Fragments arranged by size:
2241 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2883 4040 5752
Size: 274 80 33 51 1443 906 1157 1712
Cuts at: 5752 96
Size: 138
Fragments arranged by size:
1712 1443 1157 906 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 5754 5786 2159
Size: 32 3563 32 2167
Fragments arranged by size:
3563 2167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5794
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2584 2584
Size: 5794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII
SexAI SfiI SfoI SrfI SwaI