Restriction map for
pFN21AE0804
(Circular) (Six-base) MAPSORT of: pFN21AE0804.seq Check: 487 from: 1 to: 5572
pFN21AE0804 5572 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3382 278
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2915 1937
Size: 978 4594
Acc65I G'GTAC_C
Cuts at: 2893 2893
Size: 5572
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2284 3635 5019 5287 1632
Size: 652 1351 1384 268 1917
Fragments arranged by size:
1917 1384 1351 652 268
AfeI AGC'GCT
Cuts at: 2312 2312
Size: 5572
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 4471 5448 1203
Size: 3268 977 1327
Fragments arranged by size:
3268 1327 977
AgeI A'CCGG_T
Cuts at: 2083 4403 2083
Size: 2320 3252
AhdI GACnn_n'nnGTC
Cuts at: 4301 4301
Size: 5572
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5572
AlwNI CAG_nnn'CTG
Cuts at: 2671 2854 3315 4887 2671
Size: 183 461 1572 3356
Fragments arranged by size:
3356 1572 461 183
ApaI G_GGCC'C
Cuts at: 2138 2815 2138
Size: 677 4895
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2881 3107 3259 5368 1493
Size: 1388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2755 2774 2897 1870
Size: 73 132 680 19 123 4545
Fragments arranged by size:
4545 680 132 123 73 19
BamHI G'GATC_C
Cuts at: 2902 3269 2902
Size: 367 5205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 2825 2837 2893 4348
Size: 325 217 627 441 597 12 56 1455
Cuts at: 4348 618
Size: 1842
Fragments arranged by size:
1842 1455 627 597 441 325 217 56
12
BanII G_rGCy'C
Cuts at: 1391 2138 2283 2815 2891 1391
Size: 747 145 532 76 4072
Fragments arranged by size:
4072 747 532 145 76
BbeI G_GCGC'C
Cuts at: 2232 2829 2232
Size: 597 4975
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2041 2641 928
Size: 971 142 600 3859
Fragments arranged by size:
3859 971 600 142
BbvCI CC'TCA_GC
Cuts at: 2792 2792
Size: 5572
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2819 2853 3763 3797 1903
Size: 34 882 34 910 34 3678
Fragments arranged by size:
3678 910 882 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3464 4674 3464
Size: 1210 4362
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2199 2531 4183
Size: 107 122 71 631 605 527 332 1652
Cuts at: 4183 136
Size: 1525
Fragments arranged by size:
1652 1525 631 605 527 332 122 107
71
BglII A'GATC_T
Cuts at: 5567 5567
Size: 5572
BlpI GC'TnA_GC
Cuts at: 2989 2989
Size: 5572
Bme1580I G_kGCm'C
Cuts at: 1790 2138 2462 2815 3633 4789 1790
Size: 348 324 353 818 1156 2573
Fragments arranged by size:
2573 1156 818 353 348 324
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5572
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3303 4261 442
Size: 416 53 83 399 24 1886 958 1753
Fragments arranged by size:
1886 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2047 2677 4232 1239
Size: 808 630 1555 2579
Fragments arranged by size:
2579 1555 808 630
Bpu10I CC'TnA_GC
Cuts at: 2214 2792 2214
Size: 578 4994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3697 4562 4860 3697
Size: 865 298 4409
Fragments arranged by size:
4409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2870 4235 882
Size: 617 1371 1365 2219
Fragments arranged by size:
2219 1371 1365 617
BsaAI yAC'GTr
Cuts at: 493 1204 2485 3363 493
Size: 711 1281 878 2702
Fragments arranged by size:
2702 1281 878 711
BsaBI GATnn'nnATC
Cuts at: 5566 5566
Size: 5572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 2826 3379 3761
Size: 53 83 186 936 696 597 553 382
Cuts at: 3761 4396 275
Size: 635 1451
Fragments arranged by size:
1451 936 696 635 597 553 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2083 4005 4403 4677 4824 5235 2083
Size: 1922 398 274 147 411 2420
Fragments arranged by size:
2420 1922 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2720 2750 1178
Size: 30 165 30 1317 30 4000
Fragments arranged by size:
4000 1317 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5572
BseYI C'CCAG_C
Cuts at: 2833 4775 5271 2833
Size: 1942 496 3134
Fragments arranged by size:
3134 1942 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 1163
Size: 573 405 4594
Fragments arranged by size:
4594 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2373 2412 3022 3783 4811 1540
Size: 275 186 372 39 610 761 1028 2301
Fragments arranged by size:
2301 1028 761 610 372 275 186 39
BsiHKAI G_wGCw'C
Cuts at: 2106 2256 3633 3718 4789 2106
Size: 150 1377 85 1071 2889
Fragments arranged by size:
2889 1377 1071 150 85
BsmI GAATG_Cn'
Cuts at: 3083 3176 3083
Size: 93 5479
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2138 2256 2283 2462 2815 2891
Size: 399 316 32 118 27 179 353 76
Cuts at: 2891 3633 3718 4789 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 353 316 179 118
85 76 32 27
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 3461 5191 3461
Size: 1730 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2570 2927 844
Size: 1316 410 357 3489
Fragments arranged by size:
3489 1316 410 357
BsrBI CCG'CTC
Cuts at: 1394 1823 3459 1394
Size: 429 1636 3507
Fragments arranged by size:
3507 1636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4067 4241 65
Size: 1183 2819 174 1396
Fragments arranged by size:
2819 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2083 2352 2407 4216 4403 1700
Size: 110 273 269 55 1809 187 2869
Fragments arranged by size:
2869 1809 273 269 187 110 55
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5572
BssHII G'CGCG_C
Cuts at: 1925 2610 1925
Size: 685 4887
BssSI C'ACGA_G
Cuts at: 3632 4644 3632
Size: 1012 4560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2671 794
Size: 1877 3695
BstBI TT'CG_AA
Cuts at: 4376 4376
Size: 5572
BstEII G'GTnAC_C
Cuts at: 1184 2557 1184
Size: 1373 4199
BstXI CCAn_nnnn'nTGG
Cuts at: 2716 2716
Size: 5572
BstYI r'GATC_y
Cuts at: 1972 2902 3269 3654 3671 5123 5209 5221 5567
Size: 930 367 385 17 1452 86 12 346
Cuts at: 5567 1972
Size: 1977
Fragments arranged by size:
1977 1452 930 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2208 2579 2859 513
Size: 552 1143 371 280 3226
Fragments arranged by size:
3226 1143 552 371 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4833
Fragments arranged by size:
4833 683 56
BtsI GCAGTG_nn'
Cuts at: 3088 3882 3902 3088
Size: 794 20 4758
Fragments arranged by size:
4758 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2876 2876
Size: 5572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2045 4579 809
Size: 507 729 2534 1802
Fragments arranged by size:
2534 1802 729 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2370 2409
Size: 54 1425 50 275 18 333 207 39
Cuts at: 2409 2487 2706 3019 3908 5537 8
Size: 78 219 313 889 1629 43
Fragments arranged by size:
1629 1425 889 333 313 275 219 207
78 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2370 2409 3019 1537
Size: 275 558 39 610 4090
Fragments arranged by size:
4090 610 558 275 39
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 2630 3502 1353
Size: 980 297 872 3423
Fragments arranged by size:
3423 980 872 297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4159 4533 4679 387
Size: 1064 138 2570 374 146 1280
Fragments arranged by size:
2570 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2284 2677 3635 4232 5019 5287
Size: 393 415 237 393 958 597 787 268
Cuts at: 5287 1239
Size: 1524
Fragments arranged by size:
1524 958 787 597 415 393 393 268
237
EcoNI CCTnn'n_nnAGG
Cuts at: 2819 2819
Size: 5572
EcoO109I rG'GnC_Cy
Cuts at: 2134 2135 2811 2812 2134
Size: 1 676 1 4894
Fragments arranged by size:
4894 676 1 1
EcoRI G'AATT_C
Cuts at: 2881 3259 2881
Size: 378 5194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 5572
FspI TGC'GCA
Cuts at: 2447 4078 2447
Size: 1631 3941
HaeII r_GCGC'y
Cuts at: 1678 2232 2314 2829 4719 1678
Size: 554 82 515 1890 2531
Fragments arranged by size:
2531 1890 554 515 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4213 4245 4287 4319 1373
Size: 32 135 32 2641 32 42 32 2626
Fragments arranged by size:
2641 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2728 2916 3162 669
Size: 988 281 790 188 246 3079
Fragments arranged by size:
3079 988 790 281 246 188
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5572
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KasI G'GCGC_C
Cuts at: 2228 2825 2228
Size: 597 4975
KpnI G_GTAC'C
Cuts at: 2897 2897
Size: 5572
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2346 4686 4870 1305
Size: 1041 2340 184 2007
Fragments arranged by size:
2340 2007 1041 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2489 2708 10
Size: 54 1425 343 657 219 2874
Fragments arranged by size:
2874 1425 657 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3530 3889 4048 518
Size: 648 2364 359 159 2042
Fragments arranged by size:
2364 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2419 2581 2704 2791 2988 3665 4813 5058
Size: 813 162 123 87 197 677 1148 245
Cuts at: 5058 1606
Size: 2120
Fragments arranged by size:
2120 1148 813 677 245 197 162 123
87
NaeI GCC'GGC
Cuts at: 2354 2409 2354
Size: 55 5517
NarI GG'CG_CC
Cuts at: 2229 2826 2229
Size: 597 4975
NcoI C'CATG_G
Cuts at: 513 1065 2859 513
Size: 552 1794 3226
Fragments arranged by size:
3226 1794 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NgoMIV G'CCGG_C
Cuts at: 2352 2407 2352
Size: 55 5517
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5572
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2930 4475 1132
Size: 97 1701 1545 2229
Fragments arranged by size:
2229 1701 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2224 2815 1348
Size: 876 591 4105
Fragments arranged by size:
4105 876 591
PciI A'CATG_T
Cuts at: 4471 4471
Size: 5572
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5572
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PmlI CAC'GTG
Cuts at: 2485 2485
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 3142 3142
Size: 5572
PspOMI G'GGCC_C
Cuts at: 2134 2811 2134
Size: 677 4895
PstI C_TGCA'G
Cuts at: 830 2667 2924 830
Size: 1837 257 3478
Fragments arranged by size:
3478 1837 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
SacII CC_GC'GG
Cuts at: 2582 2582
Size: 5572
SalI G'TCGA_C
Cuts at: 1936 2914 1936
Size: 978 4594
SapI GCTCTTCn'nnn_
Cuts at: 2630 2630
Size: 5572
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 3820 5532 3820
Size: 1712 3860
SexAI A'CCwGG_T
Cuts at: 2498 2498
Size: 5572
SfcI C'TryA_G
Cuts at: 826 1045 2663 2920 4055 4736 4927 826
Size: 219 1618 257 1135 681 191 1471
Fragments arranged by size:
1618 1471 1135 681 257 219 191
SfoI GGC'GCC
Cuts at: 2230 2827 2230
Size: 597 4975
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5572
SmaI CCC'GGG
Cuts at: 1872 2776 2899 1872
Size: 904 123 4545
Fragments arranged by size:
4545 904 123
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3676 4577 4839 820
Size: 197 926 1733 901 262 1553
Fragments arranged by size:
1733 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2930 1132
Size: 1798 3774
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5572
StyI C'CwwG_G
Cuts at: 513 1065 2859 3011 513
Size: 552 1794 152 3074
Fragments arranged by size:
3074 1794 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2545 2635 2825 3599 3616 3769 3954
Size: 1255 965 90 190 774 17 153 185
Cuts at: 3954 4399 325
Size: 445 1498
Fragments arranged by size:
1498 1255 965 774 445 190 185 153
90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3818 5530 96
Size: 274 80 33 51 1443 1841 1712 138
Fragments arranged by size:
1841 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5532 5564 5532
Size: 32 5540
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5572
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5572
XmaI C'CCGG_G
Cuts at: 1870 2774 2897 1870
Size: 904 123 4545
Fragments arranged by size:
4545 904 123
XmnI GAAnn'nnTTC
Cuts at: 3701 3701
Size: 5572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3380 276
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII
RsrII SacI SanDI SfiI SrfI SwaI XcmI