Restriction map for
pFN21AE0803
(Circular) (Six-base) MAPSORT of: pFN21AE0803.seq Check: 3381 from: 1 to: 5641
pFN21AE0803 5641 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2478 2478
Size: 5641
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2163 2808 2984 1937
Size: 226 645 176 4594
Fragments arranged by size:
4594 645 226 176
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2746 3704 5088 5356 1632
Size: 1114 958 1384 268 1917
Fragments arranged by size:
1917 1384 1114 958 268
AfeI AGC'GCT
Cuts at: 2871 2871
Size: 5641
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 2429 1166
Size: 1263 4378
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2371 3384 4956 2371
Size: 1013 1572 3056
Fragments arranged by size:
3056 1572 1013
ApaI G_GGCC'C
Cuts at: 2452 2452
Size: 5641
ApaLI G'TGCA_C
Cuts at: 2783 3698 4854 2783
Size: 915 1156 3570
Fragments arranged by size:
3570 1156 915
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2278 2640 2966 1870
Size: 73 335 362 326 4545
Fragments arranged by size:
4545 362 335 326 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2483 2962 4417 618
Size: 325 217 627 264 432 479 1455 1842
Fragments arranged by size:
1842 1455 627 479 432 325 264 217
BanII G_rGCy'C
Cuts at: 1391 2016 2106 2452 2511 2658 2960 1391
Size: 625 90 346 59 147 302 4072
Fragments arranged by size:
4072 625 346 302 147 90 59
BbeI G_GCGC'C
Cuts at: 2055 2487 2055
Size: 432 5209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2851 4252 136
Size: 107 122 71 631 605 1179 1401 1525
Fragments arranged by size:
1525 1401 1179 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 2150 2477 2886 3058 2150
Size: 327 409 172 4733
Fragments arranged by size:
4733 409 327 172
Bme1580I G_kGCm'C
Cuts at: 1790 2452 2787 2830 3702 4858 1790
Size: 662 335 43 872 1156 2573
Fragments arranged by size:
2573 1156 872 662 335 43
BmgBI CAC'GTC
Cuts at: 1318 2197 1318
Size: 879 4762
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2488 3372 4330
Size: 416 53 83 399 24 1071 884 958
Cuts at: 4330 442
Size: 1753
Fragments arranged by size:
1753 1071 958 884 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2846 4301 1239
Size: 1607 1455 2579
Fragments arranged by size:
2579 1607 1455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2900 4304 882
Size: 617 1401 1404 2219
Fragments arranged by size:
2219 1404 1401 617
BsaAI yAC'GTr
Cuts at: 493 1204 2035 3432 493
Size: 711 831 1397 2702
Fragments arranged by size:
2702 1397 831 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2484 3448 3830
Size: 53 83 186 936 519 432 964 382
Cuts at: 3830 4465 275
Size: 635 1451
Fragments arranged by size:
1451 964 936 635 519 432 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 2666 1186
Size: 1468 12 4161
Fragments arranged by size:
4161 1468 12
BseYI C'CCAG_C
Cuts at: 2122 2386 2510 2625 2872 2890 4844 5340 2122
Size: 264 124 115 247 18 1954 496 2423
Fragments arranged by size:
2423 1954 496 264 247 124 115 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 2450 2473 1163
Size: 573 573 141 23 4331
Fragments arranged by size:
4331 573 573 141 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2352 3091 3852 4880 1540
Size: 275 186 351 739 761 1028 2301
Fragments arranged by size:
2301 1028 761 739 351 275 186
BsiHKAI G_wGCw'C
Cuts at: 2787 3702 3787 4858 2787
Size: 915 85 1071 3570
Fragments arranged by size:
3570 1071 915 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
BsmBI CGTCTCn'nnnn_
Cuts at: 2871 2871
Size: 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2106 2452 2511 2658 2787 2830
Size: 399 226 90 346 59 147 129 43
Cuts at: 2830 2960 3702 3787 4858 1391
Size: 130 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 346 226 147 130
129 90 85 59 43
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2478 2736 2996 844
Size: 1634 258 260 3489
Fragments arranged by size:
3489 1634 260 258
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2265 4285 4472 1700
Size: 110 455 2020 187 2869
Fragments arranged by size:
2869 2020 455 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 2398 3701 4713 2398
Size: 1303 1012 3326
Fragments arranged by size:
3326 1303 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 794
Size: 1679 3962
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2209 2971 3338 3723 3740 5192 5278 5290
Size: 237 762 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 762 385 367 346 237 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5089
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 2785 2830 3157 3951 3971 2372
Size: 413 45 327 794 20 4042
Fragments arranged by size:
4042 794 413 327 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DraIII CAC_nnn'GTG
Cuts at: 2236 2580 2236
Size: 344 5297
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2349 2373
Size: 54 1425 50 275 18 408 111 24
Cuts at: 2373 2775 2793 3088 3977 5606 8
Size: 402 18 295 889 1629 43
Fragments arranged by size:
1629 1425 889 408 402 295 275 111
54 50 43 24 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2349 3088 1537
Size: 275 537 739 4090
Fragments arranged by size:
4090 739 537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 2684 2690 3571 1353
Size: 1034 297 6 881 3423
Fragments arranged by size:
3423 1034 881 297 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2746 2846 3704 4301 5088 5356 1239
Size: 393 1114 100 858 597 787 268 1524
Fragments arranged by size:
1524 1114 858 787 597 393 268 100
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2096
Size: 5641
EcoO109I rG'GnC_Cy
Cuts at: 2449 2621 2920 2449
Size: 172 299 5170
Fragments arranged by size:
5170 299 172
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 2055 2487 2873 4788 1678
Size: 377 432 386 1915 2531
Fragments arranged by size:
2531 1915 432 386 377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KasI G'GCGC_C
Cuts at: 2051 2483 2051
Size: 432 5209
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 10
Size: 54 1425 343 408 3411
Fragments arranged by size:
3411 1425 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 3599 3958 4117 518
Size: 648 1263 1170 359 159 2042
Fragments arranged by size:
2042 1263 1170 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2481 2549 2629 3057 3734 4882 5127 1606
Size: 875 68 80 428 677 1148 245 2120
Fragments arranged by size:
2120 1148 875 677 428 245 80 68
NaeI GCC'GGC
Cuts at: 2267 2267
Size: 5641
NarI GG'CG_CC
Cuts at: 2052 2484 2052
Size: 432 5209
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5089
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NgoMIV G'CCGG_C
Cuts at: 2265 2265
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2651 2830 1348
Size: 1303 179 4159
Fragments arranged by size:
4159 1303 179
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 1108
Size: 1128 4513
PfoI T'CCnGG_A
Cuts at: 2190 2720 2190
Size: 530 5111
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PmlI CAC'GTG
Cuts at: 2035 2035
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2448 2448
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2164 2989 4124 4805 4996 826
Size: 219 1119 825 1135 681 191 1471
Fragments arranged by size:
1471 1135 1119 825 681 219 191
SfoI GGC'GCC
Cuts at: 2053 2485 2053
Size: 432 5209
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2280 2642 2968 1872
Size: 408 362 326 4545
Fragments arranged by size:
4545 408 362 326
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2221 3080 513
Size: 552 1156 859 3074
Fragments arranged by size:
3074 1156 859 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2507 3668 3685 3838 4023 4468 325
Size: 1255 927 1161 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1161 927 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3887 5599 96
Size: 274 80 33 51 1443 1910 1712 138
Fragments arranged by size:
1910 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 5601 5633 2019
Size: 32 3550 32 2027
Fragments arranged by size:
3550 2027 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2188 2188
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2278 2640 2966 1870
Size: 408 362 326 4545
Fragments arranged by size:
4545 408 362 326
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI