Restriction map for
pFN21AE0800
(Circular) (Six-base) MAPSORT of: pFN21AE0800.seq Check: 2873 from: 1 to: 9298
pFN21AE0800 9298 bp
With 182 enzymes: SgfI *
March 28, 2012 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7108 278
Size: 53 83 186 936 5572 2468
Fragments arranged by size:
5572 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6641 1937
Size: 4704 4594
Acc65I G'GTAC_C
Cuts at: 4376 6619 4376
Size: 2243 7055
AclI AA'CG_TT
Cuts at: 1563 7425 7798 1563
Size: 5862 373 3063
Fragments arranged by size:
5862 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3536 3954 4584 4944 5649 5757 7361 8745
Size: 1904 418 630 360 705 108 1604 1384
Cuts at: 8745 9013 1632
Size: 268 1917
Fragments arranged by size:
1917 1904 1604 1384 705 630 418 360
268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9101
AflIII A'CryG_T
Cuts at: 1203 2648 2820 2977 3081 4476 8197 9174 1203
Size: 1445 172 157 104 1395 3721 977 1327
Fragments arranged by size:
3721 1445 1395 1327 977 172 157 104
AgeI A'CCGG_T
Cuts at: 6293 8129 6293
Size: 1836 7462
AhdI GACnn_n'nnGTC
Cuts at: 2484 8027 2484
Size: 5543 3755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9298
AlwNI CAG_nnn'CTG
Cuts at: 4259 5366 7041 8613 4259
Size: 1107 1675 1572 4944
Fragments arranged by size:
4944 1675 1572 1107
ApaLI G'TGCA_C
Cuts at: 7355 8511 7355
Size: 1156 8142
ApoI r'AATT_y
Cuts at: 1493 2531 3194 3315 3719 3785 4126 4324 4904
Size: 1038 663 121 404 66 341 198 580
Cuts at: 4904 5978 6346 6607 6833 6985 9094 1493
Size: 1074 368 261 226 152 2109 1697
Fragments arranged by size:
2109 1697 1074 1038 663 580 404 368
341 261 226 198 152 121 66
AseI AT'TA_AT
Cuts at: 160 1032 3666 5222 7852 160
Size: 872 2634 1556 2630 1606
Fragments arranged by size:
2634 2630 1606 1556 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9298
AvaI C'yCGr_G
Cuts at: 1870 1943 6528 6623 1870
Size: 73 4585 95 4545
Fragments arranged by size:
4585 4545 95 73
AvrII C'CTAG_G
Cuts at: 5116 5116
Size: 9298
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4884 4917 4884
Size: 33 9265
BamHI G'GATC_C
Cuts at: 2747 5257 6334 6628 6995 2747
Size: 2510 1077 294 367 5050
Fragments arranged by size:
5050 2510 1077 367 294
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3854 4376 4841 6296 6619
Size: 325 217 627 2067 522 465 1455 323
Cuts at: 6619 8074 618
Size: 1455 1842
Fragments arranged by size:
2067 1842 1455 1455 627 522 465 325
323 217
BanII G_rGCy'C
Cuts at: 1391 6617 1391
Size: 5226 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3542 3596 4027 4260 4856 4960 5517
Size: 971 1643 54 431 233 596 104 557
Cuts at: 5517 6168 6263 6270 928
Size: 651 95 7 3956
Fragments arranged by size:
3956 1643 971 651 596 557 431 233
104 95 54 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7489 7523 1903
Size: 34 5552 34 3678
Fragments arranged by size:
5552 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3920 7190 8400 3920
Size: 3270 1210 4818
Fragments arranged by size:
4818 3270 1210
BclI T'GATC_A
Cuts at: 1548 2995 4471 1548
Size: 1447 1476 6375
Fragments arranged by size:
6375 1476 1447
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7909 136
Size: 107 122 71 631 605 6237 1525
Fragments arranged by size:
6237 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4142 9293 4142
Size: 5151 4147
BlpI GC'TnA_GC
Cuts at: 4433 6715 4433
Size: 2282 7016
Bme1580I G_kGCm'C
Cuts at: 1790 3857 7359 8515 1790
Size: 2067 3502 1156 2573
Fragments arranged by size:
3502 2573 2067 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3253 4587 6221
Size: 416 53 83 399 24 1836 1334 1634
Cuts at: 6221 7029 7987 442
Size: 808 958 1753
Fragments arranged by size:
1836 1753 1634 1334 958 808 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3825 3825
Size: 9298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3734 7958 1239
Size: 2495 4224 2579
Fragments arranged by size:
4224 2579 2495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2606 5733 7423 8288 8586 2606
Size: 3127 1690 865 298 3318
Fragments arranged by size:
3318 3127 1690 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7961 882
Size: 617 6462 2219
Fragments arranged by size:
6462 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5020 7089 493
Size: 711 3816 2069 2702
Fragments arranged by size:
3816 2702 2069 711
BsaBI GATnn'nnATC
Cuts at: 5256 9292 5256
Size: 4036 5262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7105 7487 8122 275
Size: 53 83 186 936 5572 382 635 1451
Fragments arranged by size:
5572 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2335 6293 7731 8129 8403 8550 8961 2335
Size: 3958 1438 398 274 147 411 2672
Fragments arranged by size:
3958 2672 1438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 9073
Fragments arranged by size:
9073 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4848 6516 1186
Size: 3662 1668 3968
Fragments arranged by size:
3968 3662 1668
BseYI C'CCAG_C
Cuts at: 2178 2416 5283 8501 8997 2178
Size: 238 2867 3218 496 2479
Fragments arranged by size:
3218 2867 2479 496 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8725
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6748 7509 8537 1540
Size: 275 186 4747 761 1028 2301
Fragments arranged by size:
4747 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 7359 7444 8515 7359
Size: 85 1071 8142
Fragments arranged by size:
8142 1071 85
BsmI GAATG_Cn'
Cuts at: 5127 6809 6902 5127
Size: 1682 93 7523
Fragments arranged by size:
7523 1682 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3857 6617 7359 7444 8515 1391
Size: 399 2067 2760 742 85 1071 2174
Fragments arranged by size:
2760 2174 2067 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2335 8961 2335
Size: 6626 2672
BspHI T'CATG_A
Cuts at: 7187 8917 7187
Size: 1730 7568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6653 844
Size: 5809 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 7185 1394
Size: 429 5362 3507
Fragments arranged by size:
5362 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2018 2952 7793 7967 65
Size: 1183 770 934 4841 174 1396
Fragments arranged by size:
4841 1396 1183 934 770 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6293 7942 8129 1700
Size: 110 4483 1649 187 2869
Fragments arranged by size:
4483 2869 1649 187 110
BsrGI T'GTAC_A
Cuts at: 96 2247 96
Size: 2151 7147
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9298
BssSI C'ACGA_G
Cuts at: 5940 7358 8370 5940
Size: 1418 1012 6868
Fragments arranged by size:
6868 1418 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9298
BstBI TT'CG_AA
Cuts at: 6452 8102 6452
Size: 1650 7648
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9298
BstXI CCAn_nnnn'nTGG
Cuts at: 3406 4247 3406
Size: 841 8457
BstYI r'GATC_y
Cuts at: 1972 2747 4142 5257 6334 6628 6995 7380 7397
Size: 775 1395 1115 1077 294 367 385 17
Cuts at: 7397 8849 8935 8947 9293 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1395 1115 1077 775 385 367
346 294 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4047 4047
Size: 9298
BtgI C'CryG_G
Cuts at: 513 1065 2004 4542 5954 513
Size: 552 939 2538 1412 3857
Fragments arranged by size:
3857 2538 1412 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8559
Fragments arranged by size:
8559 683 56
BtsI GCAGTG_nn'
Cuts at: 5917 6169 6814 7608 7628 5917
Size: 252 645 794 20 7587
Fragments arranged by size:
7587 794 645 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9263
DraI TTT'AAA
Cuts at: 2683 5031 6602 2683
Size: 2348 1571 5379
Fragments arranged by size:
5379 2348 1571
DraIII CAC_nnn'GTG
Cuts at: 3366 3366
Size: 9298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8305 809
Size: 507 6989 1802
Fragments arranged by size:
6989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6745 7634 9263
Size: 54 1425 50 275 18 4915 889 1629
Cuts at: 9263 8
Size: 43
Fragments arranged by size:
4915 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6745 1537
Size: 275 4933 4090
Fragments arranged by size:
4933 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2596 2722 3699 3786 3794 5310 5867 6567
Size: 1243 126 977 87 8 1516 557 700
Cuts at: 6567 7228 1353
Size: 661 3423
Fragments arranged by size:
3423 1516 1243 977 700 661 557 126
87 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2129 7885 8259 8405 387
Size: 1064 138 540 5756 374 146 1280
Fragments arranged by size:
5756 1280 1064 540 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3536 3734 3954 4584 4944 5649 5757
Size: 393 1904 198 220 630 360 705 108
Cuts at: 5757 7361 7958 8745 9013 1239
Size: 1604 597 787 268 1524
Fragments arranged by size:
1904 1604 1524 787 705 630 597 393
360 268 220 198 108
EcoNI CCTnn'n_nnAGG
Cuts at: 3383 3462 5589 3383
Size: 79 2127 7092
Fragments arranged by size:
7092 2127 79
EcoO109I rG'GnC_Cy
Cuts at: 2299 2299
Size: 9298
EcoRI G'AATT_C
Cuts at: 3315 6346 6607 6985 3315
Size: 3031 261 378 5628
Fragments arranged by size:
5628 3031 378 261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2789 5597 6294 2789
Size: 2808 697 5793
Fragments arranged by size:
5793 2808 697
FspI TGC'GCA
Cuts at: 7804 7804
Size: 9298
HaeII r_GCGC'y
Cuts at: 1678 8445 1678
Size: 6767 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2470 2502 3532 3564 3894
Size: 32 135 32 898 32 1030 32 330
Cuts at: 3894 3926 3967 3999 4843 4875 5882 5914 6413
Size: 32 41 32 844 32 1007 32 499
Cuts at: 6413 6445 6497 6529 7939 7971 8013 8045 1373
Size: 32 52 32 1410 32 42 32 2626
Fragments arranged by size:
2626 1410 1030 1007 898 844 499 330
135 52 42 41 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2050 2947 3999 6642 6888 669
Size: 988 281 112 897 1052 2643 246 3079
Fragments arranged by size:
3079 2643 1052 988 897 281 246 112
HindIII A'AGCT_T
Cuts at: 748 3489 748
Size: 2741 6557
HpaI GTT'AAC
Cuts at: 3999 6888 3999
Size: 2889 6409
KpnI G_GTAC'C
Cuts at: 4380 6623 4380
Size: 2243 7055
MfeI C'AATT_G
Cuts at: 2120 2328 3182 6897 2120
Size: 208 854 3715 4521
Fragments arranged by size:
4521 3715 854 208
MluI A'CGCG_T
Cuts at: 9174 9174
Size: 9298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 4933 5031 5557 8412 8596 1305
Size: 1515 2113 98 526 2855 184 2007
Fragments arranged by size:
2855 2113 2007 1515 526 184 98
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7476
Fragments arranged by size:
7476 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4281 5681 7256 7615 7774 518
Size: 648 843 2272 1400 1575 359 159 2042
Fragments arranged by size:
2272 2042 1575 1400 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3290 6518 6714 7391 8539 8784 1606
Size: 1684 3228 196 677 1148 245 2120
Fragments arranged by size:
3228 2120 1684 1148 677 245 196
NcoI C'CATG_G
Cuts at: 513 1065 2004 4542 513
Size: 552 939 2538 5269
Fragments arranged by size:
5269 2538 939 552
NdeI CA'TA_TG
Cuts at: 387 2734 6501 387
Size: 2347 3767 3184
Fragments arranged by size:
3767 3184 2347
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9298
NotI GC'GGCC_GC
Cuts at: 6745 6745
Size: 9298
NspI r_CATG'y
Cuts at: 1132 1229 2652 2824 2981 3085 3180 4995 6656
Size: 97 1423 172 157 104 95 1815 1661
Cuts at: 6656 8201 1132
Size: 1545 2229
Fragments arranged by size:
2229 1815 1661 1545 1423 172 157 104
97 95
PacI TTA_AT'TAA
Cuts at: 5226 5226
Size: 9298
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9298
PciI A'CATG_T
Cuts at: 2648 2820 2977 3081 8197 2648
Size: 172 157 104 5116 3749
Fragments arranged by size:
5116 3749 172 157 104
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9298
PmeI GTTT'AAAC
Cuts at: 6602 6602
Size: 9298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7367 7399 8880 8912 7367
Size: 32 1481 32 7753
Fragments arranged by size:
7753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2299
Size: 9298
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9298
PsiI TTA'TAA
Cuts at: 6868 6868
Size: 9298
PstI C_TGCA'G
Cuts at: 830 5441 6650 830
Size: 4611 1209 3478
Fragments arranged by size:
4611 3478 1209
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9298
PvuII CAG'CTG
Cuts at: 3290 6518 3290
Size: 3228 6070
SalI G'TCGA_C
Cuts at: 1936 6640 1936
Size: 4704 4594
SanDI GG'GwC_CC
Cuts at: 2299 2299
Size: 9298
SapI GCTCTTCn'nnn_
Cuts at: 2596 2722 2596
Size: 126 9172
SbfI CC_TGCA'GG
Cuts at: 6650 6650
Size: 9298
ScaI AGT'ACT
Cuts at: 7546 9258 7546
Size: 1712 7586
SexAI A'CCwGG_T
Cuts at: 4174 5812 4174
Size: 1638 7660
SfcI C'TryA_G
Cuts at: 826 1045 2218 4873 5437 6646 7781 8462 8653
Size: 219 1173 2655 564 1209 1135 681 191
Cuts at: 8653 826
Size: 1471
Fragments arranged by size:
2655 1471 1209 1173 1135 681 564 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9298
SmaI CCC'GGG
Cuts at: 1872 6625 1872
Size: 4753 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2621 5748 7402 8303 8565 820
Size: 197 926 678 3127 1654 901 262 1553
Fragments arranged by size:
3127 1654 1553 926 901 678 262 197
SnaBI TAC'GTA
Cuts at: 493 7089 493
Size: 6596 2702
SpeI A'CTAG_T
Cuts at: 152 4982 152
Size: 4830 4468
SphI G_CATG'C
Cuts at: 1132 6656 1132
Size: 5524 3774
SspI AAT'ATT
Cuts at: 5 52 3839 3923 5090 7222 5
Size: 47 3787 84 1167 2132 2081
Fragments arranged by size:
3787 2132 2081 1167 84 47
StuI AGG'CCT
Cuts at: 1517 3420 5270 5775 1517
Size: 1903 1850 505 5040
Fragments arranged by size:
5040 1903 1850 505
StyI C'CwwG_G
Cuts at: 513 1065 2004 2964 3421 4542 5116 5415 5553
Size: 552 939 960 457 1121 574 299 138
Cuts at: 5553 5604 6477 6737 513
Size: 51 873 260 3074
Fragments arranged by size:
3074 1121 960 939 873 574 552 457
299 260 138 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3235 4569 7325 7342 7495 7680 8125
Size: 1255 1655 1334 2756 17 153 185 445
Cuts at: 8125 325
Size: 1498
Fragments arranged by size:
2756 1655 1498 1334 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 3555 7544
Size: 274 80 33 51 1443 270 1308 3989
Cuts at: 7544 9256 96
Size: 1712 138
Fragments arranged by size:
3989 1712 1443 1308 274 270 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2221 2253 2317 2349 9258 9290 2221
Size: 32 64 32 6909 32 2229
Fragments arranged by size:
6909 2229 64 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9298
XbaI T'CTAG_A
Cuts at: 4822 6634 4822
Size: 1812 7486
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2536 4312 5499 2536
Size: 1776 1187 6335
Fragments arranged by size:
6335 1776 1187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9298
XmaI C'CCGG_G
Cuts at: 1870 6623 1870
Size: 4753 4545
XmnI GAAnn'nnTTC
Cuts at: 2570 4818 7427 2570
Size: 2248 2609 4441
Fragments arranged by size:
4441 2609 2248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7106 276
Size: 53 83 186 936 5572 2468
Fragments arranged by size:
5572 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PacI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI
SrfI SwaI