Restriction map for
pFN21AE0787
(Circular) (Six-base) MAPSORT of: pFN21AE0787.seq Check: 3836 from: 1 to: 5764
pFN21AE0787 5764 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2189 2189
Size: 5764
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 3827 5211 5479 1632
Size: 614 1581 1384 268 1917
Fragments arranged by size:
1917 1581 1384 614 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 4663 5640 1203
Size: 3460 977 1327
Fragments arranged by size:
3460 1327 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 2102 2747 1166
Size: 936 645 4183
Fragments arranged by size:
4183 936 645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AlwNI CAG_nnn'CTG
Cuts at: 2344 2644 3507 5079 2344
Size: 300 863 1572 3029
Fragments arranged by size:
3029 1572 863 300
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 3073 3299 3451 5560 1493
Size: 1580 226 152 2109 1697
Fragments arranged by size:
2109 1697 1580 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 2268 3089 1870
Size: 73 325 821 4545
Fragments arranged by size:
4545 821 325 73
BamHI G'GATC_C
Cuts at: 2154 2824 3094 3461 2154
Size: 670 270 367 4457
Fragments arranged by size:
4457 670 367 270
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3085 4540 618
Size: 325 217 627 1298 1455 1842
Fragments arranged by size:
1842 1455 1298 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3083 1391
Size: 1692 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2234 928
Size: 971 335 4458
Fragments arranged by size:
4458 971 335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3955 3989 1903
Size: 34 2018 34 3678
Fragments arranged by size:
3678 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4375 136
Size: 107 122 71 631 605 2703 1525
Fragments arranged by size:
2703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3024 3181 3024
Size: 157 5607
Bme1580I G_kGCm'C
Cuts at: 1790 3825 4981 1790
Size: 2035 1156 2573
Fragments arranged by size:
2573 2035 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2946 3495 4453
Size: 416 53 83 399 24 1529 549 958
Cuts at: 4453 442
Size: 1753
Fragments arranged by size:
1753 1529 958 549 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2276 2558 4424 1239
Size: 974 63 282 1866 2579
Fragments arranged by size:
2579 1866 974 282 63
Bpu10I CC'TnA_GC
Cuts at: 2720 2720
Size: 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2138 3889 4754 5052 2006
Size: 132 1751 865 298 2718
Fragments arranged by size:
2718 1751 865 298 132
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3555 493
Size: 711 2351 2702
Fragments arranged by size:
2702 2351 711
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3571 3953 4588 275
Size: 53 83 186 936 2038 382 635 1451
Fragments arranged by size:
2038 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2426 4197 4595 4869 5016 5427 2426
Size: 1771 398 274 147 411 2763
Fragments arranged by size:
2763 1771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5539
Fragments arranged by size:
5539 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2288 2479 2585 1186
Size: 1018 84 191 106 4365
Fragments arranged by size:
4365 1018 191 106 84
BseYI C'CCAG_C
Cuts at: 4967 5463 4967
Size: 496 5268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2155 2330 1163
Size: 573 419 175 4597
Fragments arranged by size:
4597 573 419 175
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2210 3214 3975 5003 1540
Size: 275 186 209 1004 761 1028 2301
Fragments arranged by size:
2301 1028 1004 761 275 209 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2726 3825 3910 4981 2022
Size: 704 1099 85 1071 2805
Fragments arranged by size:
2805 1099 1071 704 85
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2726 3083 3825 3910 4981 1391
Size: 399 232 704 357 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 704 399 357 232 85
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2189 3119 844
Size: 1345 930 3489
Fragments arranged by size:
3489 1345 930
BsrBI CCG'CTC
Cuts at: 1394 1823 2212 2278 3651 1394
Size: 429 389 66 1373 3507
Fragments arranged by size:
3507 1373 429 389 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 4259 4433 65
Size: 1183 3011 174 1396
Fragments arranged by size:
3011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4408 4595 1700
Size: 110 2598 187 2869
Fragments arranged by size:
2869 2598 187 110
BsrGI T'GTAC_A
Cuts at: 96 2664 96
Size: 2568 3196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 2903 3824 4836 2903
Size: 921 1012 3831
Fragments arranged by size:
3831 1012 921
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5764
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 2545 1184
Size: 1361 4403
BstXI CCAn_nnnn'nTGG
Cuts at: 2536 2566 2536
Size: 30 5734
BstYI r'GATC_y
Cuts at: 1972 2154 2626 2824 3094 3461 3846 3863 5315
Size: 182 472 198 270 367 385 17 1452
Cuts at: 5315 5401 5413 5759 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 472 385 367 346 270 198
182 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2582 2582
Size: 5764
BtgI C'CryG_G
Cuts at: 513 1065 2004 2241 513
Size: 552 939 237 4036
Fragments arranged by size:
4036 939 552 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2089 2378 487
Size: 683 56 863 289 3873
Fragments arranged by size:
3873 863 683 289 56
BtsI GCAGTG_nn'
Cuts at: 3280 4074 4094 3280
Size: 794 20 4950
Fragments arranged by size:
4950 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2526 2631 2766
Size: 54 1425 50 275 18 696 105 135
Cuts at: 2766 3211 4100 5729 8
Size: 445 889 1629 43
Fragments arranged by size:
1629 1425 889 696 445 275 135 105
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2856 2895 2972 3694 1353
Size: 1503 39 77 722 3423
Fragments arranged by size:
3423 1503 722 77 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2246 2276 2558 3827 4424 5211
Size: 393 581 33 30 282 1269 597 787
Cuts at: 5211 5479 1239
Size: 268 1524
Fragments arranged by size:
1524 1269 787 597 581 393 282 268
33 30
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 5764
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 4911 1678
Size: 3233 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 2199 2231 4405
Size: 32 135 32 495 32 100 32 2174
Cuts at: 4405 4437 4479 4511 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2174 495 135 100 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3108 3354 669
Size: 988 281 1170 246 3079
Fragments arranged by size:
3079 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2114 748
Size: 1366 4398
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2108 2538 4878 5062 1305
Size: 803 430 2340 184 2007
Fragments arranged by size:
2340 2007 803 430 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2768 10
Size: 54 1425 343 936 3006
Fragments arranged by size:
3006 1425 936 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2564 2747 2844 3722 4081 4240
Size: 648 936 462 183 97 878 359 159
Cuts at: 4240 518
Size: 2042
Fragments arranged by size:
2042 936 878 648 462 359 183 159
97
MspA1I CmG'CkG
Cuts at: 1606 2344 3180 3857 5005 5250 1606
Size: 738 836 677 1148 245 2120
Fragments arranged by size:
2120 1148 836 738 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 3122 4667 1132
Size: 97 1893 1545 2229
Fragments arranged by size:
2229 1893 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2879 1108
Size: 1771 3993
PfoI T'CCnGG_A
Cuts at: 2160 2253 2643 2160
Size: 93 390 5281
Fragments arranged by size:
5281 390 93
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PstI C_TGCA'G
Cuts at: 830 3116 830
Size: 2286 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2344 2344
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 2249 4012 5724 2249
Size: 1763 1712 2289
Fragments arranged by size:
2289 1763 1712
SfcI C'TryA_G
Cuts at: 826 1045 3112 4247 4928 5119 826
Size: 219 2067 1135 681 191 1471
Fragments arranged by size:
2067 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 3091 1872
Size: 1219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 2117 2268 3868 4769 5031
Size: 197 926 78 96 151 1600 901 262
Cuts at: 5031 820
Size: 1553
Fragments arranged by size:
1600 1553 926 901 262 197 151 96
78
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 2261 2587 1517
Size: 744 326 4694
Fragments arranged by size:
4694 744 326
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 2703 3203 513
Size: 552 939 141 558 500 3074
Fragments arranged by size:
3074 939 558 552 500 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2582 2867 3791 3808 3961 4146 4591
Size: 1255 1002 285 924 17 153 185 445
Cuts at: 4591 325
Size: 1498
Fragments arranged by size:
1498 1255 1002 924 445 285 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2247 2664
Size: 274 80 33 51 1443 129 141 417
Cuts at: 2664 4010 5722 96
Size: 1346 1712 138
Fragments arranged by size:
1712 1443 1346 417 274 141 138 129
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5724 5756 5724
Size: 32 5732
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XhoI C'TCGA_G
Cuts at: 1943 2268 1943
Size: 325 5439
XmaI C'CCGG_G
Cuts at: 1870 3089 1870
Size: 1219 4545
XmnI GAAnn'nnTTC
Cuts at: 2434 3893 2434
Size: 1459 4305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI