Restriction map for
pFN21AE0786
(Circular) (Six-base) MAPSORT of: pFN21AE0786.seq Check: 4504 from: 1 to: 6076
pFN21AE0786 6076 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3284 3284
Size: 6076
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3886 278
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3419 1937
Size: 1482 4594
Acc65I G'GTAC_C
Cuts at: 3397 3397
Size: 6076
AclI AA'CG_TT
Cuts at: 1563 4203 4576 1563
Size: 2640 373 3063
Fragments arranged by size:
3063 2640 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 3092 3244 4139 5523 5791 1632
Size: 953 507 152 895 1384 268 1917
Fragments arranged by size:
1917 1384 953 895 507 268 152
AfeI AGC'GCT
Cuts at: 2702 2702
Size: 6076
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5879
AflIII A'CryG_T
Cuts at: 1203 2901 4975 5952 1203
Size: 1698 2074 977 1327
Fragments arranged by size:
2074 1698 1327 977
AgeI A'CCGG_T
Cuts at: 4907 4907
Size: 6076
AhdI GACnn_n'nnGTC
Cuts at: 2420 4805 2420
Size: 2385 3691
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2814 1184
Size: 1630 4446
AlwNI CAG_nnn'CTG
Cuts at: 2446 3819 5391 2446
Size: 1373 1572 3131
Fragments arranged by size:
3131 1572 1373
ApaI G_GGCC'C
Cuts at: 2257 2257
Size: 6076
ApaLI G'TGCA_C
Cuts at: 3089 4133 5289 3089
Size: 1044 1156 3876
Fragments arranged by size:
3876 1156 1044
ApoI r'AATT_y
Cuts at: 1493 3385 3611 3763 5872 1493
Size: 1892 226 152 2109 1697
Fragments arranged by size:
2109 1892 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4630 160
Size: 872 3598 1606
Fragments arranged by size:
3598 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
AvaI C'yCGr_G
Cuts at: 1870 1943 3401 1870
Size: 73 1458 4545
Fragments arranged by size:
4545 1458 73
BamHI G'GATC_C
Cuts at: 3406 3773 3406
Size: 367 5709
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2365 2528 2581 2809 2978
Size: 325 217 627 578 163 53 228 169
Cuts at: 2978 3397 4852 618
Size: 419 1455 1842
Fragments arranged by size:
1842 1455 627 578 419 325 228 217
169 163 53
BanII G_rGCy'C
Cuts at: 1391 2257 2631 3395 1391
Size: 866 374 764 4072
Fragments arranged by size:
4072 866 764 374
BbeI G_GCGC'C
Cuts at: 2369 2982 2369
Size: 613 5463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5105
BbvCI CC'TCA_GC
Cuts at: 2234 2246 2234
Size: 12 6064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2687 2710 2721 2744 4267 4301 1903
Size: 34 750 23 11 23 1523 34 3678
Fragments arranged by size:
3678 1523 750 34 34 23 23 11
BciVI GTATCCnnnnn_n'
Cuts at: 3968 5178 3968
Size: 1210 4866
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6076
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 4687 136
Size: 107 122 71 631 605 463 2552 1525
Fragments arranged by size:
2552 1525 631 605 463 122 107 71
BglII A'GATC_T
Cuts at: 6071 6071
Size: 6076
BlpI GC'TnA_GC
Cuts at: 2156 3493 2156
Size: 1337 4739
Bme1580I G_kGCm'C
Cuts at: 1790 2085 2257 2586 3093 3162 4137 5293 1790
Size: 295 172 329 507 69 975 1156 2573
Fragments arranged by size:
2573 1156 975 507 329 295 172 69
BmgBI CAC'GTC
Cuts at: 1318 3007 1318
Size: 1689 4387
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 3807 4765
Size: 416 53 83 399 24 741 1649 958
Cuts at: 4765 442
Size: 1753
Fragments arranged by size:
1753 1649 958 741 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 3266 4736 1239
Size: 809 1218 1470 2579
Fragments arranged by size:
2579 1470 1218 809
Bpu10I CC'TnA_GC
Cuts at: 2234 2246 2234
Size: 12 6064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4201 5066 5364 4201
Size: 865 298 4913
Fragments arranged by size:
4913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4739 882
Size: 617 3240 2219
Fragments arranged by size:
3240 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3088 3867 493
Size: 711 1884 779 2702
Fragments arranged by size:
2702 1884 779 711
BsaBI GATnn'nnATC
Cuts at: 6070 6070
Size: 6076
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2366 2979 3119 3883
Size: 53 83 186 936 833 613 140 764
Cuts at: 3883 4265 4900 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 833 764 635 613 382 186
140 83 53
BsaWI w'CCGG_w
Cuts at: 3126 4509 4907 5181 5328 5739 3126
Size: 1383 398 274 147 411 3463
Fragments arranged by size:
3463 1383 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2124 2154 2394 2424 2909
Size: 30 165 30 721 30 240 30 485
Cuts at: 2909 2939 1178
Size: 30 4315
Fragments arranged by size:
4315 721 485 240 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2570 2930 2984 3164 1186
Size: 1384 360 54 180 4098
Fragments arranged by size:
4098 1384 360 180 54
BseYI C'CCAG_C
Cuts at: 2256 2327 2599 2605 2651 2893 3020 3230 5279
Size: 71 272 6 46 242 127 210 2049
Cuts at: 5279 5775 2256
Size: 496 2557
Fragments arranged by size:
2557 2049 496 272 242 210 127 71
46 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2810 1163
Size: 573 1074 4429
Fragments arranged by size:
4429 1074 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2417 2474 2480 3526 4287 5315
Size: 275 186 416 57 6 1046 761 1028
Cuts at: 5315 1540
Size: 2301
Fragments arranged by size:
2301 1046 1028 761 416 275 186 57
6
BsiHKAI G_wGCw'C
Cuts at: 2562 2726 3093 4137 4222 5293 2562
Size: 164 367 1044 85 1071 3345
Fragments arranged by size:
3345 1071 1044 367 164 85
BsmI GAATG_Cn'
Cuts at: 2179 3587 3680 2179
Size: 1408 93 4575
Fragments arranged by size:
4575 1408 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2338 2338
Size: 6076
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2257 2562 2586 2631 2726 3093
Size: 399 295 172 305 24 45 95 367
Cuts at: 3093 3162 3395 4137 4222 5293 1391
Size: 69 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 305 295 233
172 95 85 69 45 24
BspEI T'CCGG_A
Cuts at: 3126 5739 3126
Size: 2613 3463
BspHI T'CATG_A
Cuts at: 3965 5695 3965
Size: 1730 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3284 3431 844
Size: 2440 147 3489
Fragments arranged by size:
3489 2440 147
BsrBI CCG'CTC
Cuts at: 1394 1823 2410 3963 1394
Size: 429 587 1553 3507
Fragments arranged by size:
3507 1553 587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4571 4745 65
Size: 1183 3323 174 1396
Fragments arranged by size:
3323 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2260 2749 4720 4907 1700
Size: 110 450 489 1971 187 2869
Fragments arranged by size:
2869 1971 489 450 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6076
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6076
BssSI C'ACGA_G
Cuts at: 4136 5148 4136
Size: 1012 5064
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3201 794
Size: 2407 3669
BstBI TT'CG_AA
Cuts at: 4880 4880
Size: 6076
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6076
BstXI CCAn_nnnn'nTGG
Cuts at: 2043 2043
Size: 6076
BstYI r'GATC_y
Cuts at: 1972 2211 3406 3773 4158 4175 5627 5713 5725
Size: 239 1195 367 385 17 1452 86 12
Cuts at: 5725 6071 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1195 385 367 346 239 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 2298 2309 2474 3205 513
Size: 552 939 135 159 11 165 731 3384
Fragments arranged by size:
3384 939 731 552 165 159 135 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5337
Fragments arranged by size:
5337 683 56
BtsI GCAGTG_nn'
Cuts at: 3091 3592 4386 4406 3091
Size: 501 794 20 4761
Fragments arranged by size:
4761 794 501 20
ClaI AT'CG_AT
Cuts at: 1554 2163 1554
Size: 609 5467
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6041
DraI TTT'AAA
Cuts at: 3380 3380
Size: 6076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2491 5083 809
Size: 507 1175 2592 1802
Fragments arranged by size:
2592 1802 1175 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2471 2477
Size: 54 1425 50 275 18 306 335 6
Cuts at: 2477 2751 3523 4412 6041 8
Size: 274 772 889 1629 43
Fragments arranged by size:
1629 1425 889 772 335 306 275 274
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2471 2477 3523 1537
Size: 275 659 6 1046 4090
Fragments arranged by size:
4090 1046 659 275 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2946 4006 1353
Size: 1593 1060 3423
Fragments arranged by size:
3423 1593 1060
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2278 4663 5037 5183 387
Size: 1064 138 689 2385 374 146 1280
Fragments arranged by size:
2385 1280 1064 689 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2585 3092 3244 3266 4139 4736
Size: 393 416 537 507 152 22 873 597
Cuts at: 4736 5523 5791 1239
Size: 787 268 1524
Fragments arranged by size:
1524 873 787 597 537 507 416 393
268 152 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2951 2951
Size: 6076
EcoO109I rG'GnC_Cy
Cuts at: 2253 2944 3135 3250 2253
Size: 691 191 115 5079
Fragments arranged by size:
5079 691 191 115
EcoRI G'AATT_C
Cuts at: 3385 3763 3385
Size: 378 5698
FspI TGC'GCA
Cuts at: 4582 4582
Size: 6076
HaeII r_GCGC'y
Cuts at: 1678 2233 2369 2388 2704 2894 2982 5223 1678
Size: 555 136 19 316 190 88 2241 2531
Fragments arranged by size:
2531 2241 555 316 190 136 88 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2394 2406 2426 2438 4717
Size: 32 135 32 822 12 20 12 2279
Cuts at: 4717 4749 4791 4823 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2279 822 135 42 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3420 3666 669
Size: 988 281 1482 246 3079
Fragments arranged by size:
3079 1482 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6076
HpaI GTT'AAC
Cuts at: 3666 3666
Size: 6076
KasI G'GCGC_C
Cuts at: 2365 2978 2365
Size: 613 5463
KpnI G_GTAC'C
Cuts at: 3401 3401
Size: 6076
MfeI C'AATT_G
Cuts at: 3675 3675
Size: 6076
MluI A'CGCG_T
Cuts at: 2901 5952 2901
Size: 3051 3025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2437 5190 5374 1305
Size: 1132 2753 184 2007
Fragments arranged by size:
2753 2007 1132 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4254
Fragments arranged by size:
4254 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3210 4034 4393 4552 518
Size: 648 2044 824 359 159 2042
Fragments arranged by size:
2044 2042 824 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2300 2311 2378 2419 2443 2476 2767 2798
Size: 694 11 67 41 24 33 291 31
Cuts at: 2798 3024 3264 3492 4169 5317 5562 1606
Size: 226 240 228 677 1148 245 2120
Fragments arranged by size:
2120 1148 694 677 291 245 240 228
226 67 41 33 31 24 11
NaeI GCC'GGC
Cuts at: 2751 2751
Size: 6076
NarI GG'CG_CC
Cuts at: 2366 2979 2366
Size: 613 5463
NcoI C'CATG_G
Cuts at: 513 1065 2004 3205 513
Size: 552 939 1201 3384
Fragments arranged by size:
3384 1201 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6076
NgoMIV G'CCGG_C
Cuts at: 2749 2749
Size: 6076
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6076
NotI GC'GGCC_GC
Cuts at: 2471 3523 2471
Size: 1052 5024
NspI r_CATG'y
Cuts at: 1132 1229 3434 4979 1132
Size: 97 2205 1545 2229
Fragments arranged by size:
2229 2205 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2673 3253 1348
Size: 1325 580 4171
Fragments arranged by size:
4171 1325 580
PciI A'CATG_T
Cuts at: 4975 4975
Size: 6076
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6076
PfoI T'CCnGG_A
Cuts at: 2946 3243 2946
Size: 297 5779
PmeI GTTT'AAAC
Cuts at: 3380 3380
Size: 6076
PmlI CAC'GTG
Cuts at: 3088 3088
Size: 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 5658 5690 4145
Size: 32 1481 32 4531
Fragments arranged by size:
4531 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2944 2944
Size: 6076
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6076
PsiI TTA'TAA
Cuts at: 3646 3646
Size: 6076
PspOMI G'GGCC_C
Cuts at: 2253 2253
Size: 6076
PstI C_TGCA'G
Cuts at: 830 2772 3428 830
Size: 1942 656 3478
Fragments arranged by size:
3478 1942 656
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6076
PvuII CAG'CTG
Cuts at: 2798 3024 3264 2798
Size: 226 240 5610
Fragments arranged by size:
5610 240 226
SacII CC_GC'GG
Cuts at: 2301 2312 2477 2301
Size: 11 165 5900
Fragments arranged by size:
5900 165 11
SalI G'TCGA_C
Cuts at: 1936 3418 1936
Size: 1482 4594
SbfI CC_TGCA'GG
Cuts at: 3428 3428
Size: 6076
ScaI AGT'ACT
Cuts at: 4324 6036 4324
Size: 1712 4364
SexAI A'CCwGG_T
Cuts at: 2883 2883
Size: 6076
SfcI C'TryA_G
Cuts at: 826 1045 2768 3424 4559 5240 5431 826
Size: 219 1723 656 1135 681 191 1471
Fragments arranged by size:
1723 1471 1135 681 656 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2135 2135
Size: 6076
SfoI GGC'GCC
Cuts at: 2367 2980 2367
Size: 613 5463
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6076
SmaI CCC'GGG
Cuts at: 1872 3403 1872
Size: 1531 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4180 5081 5343 820
Size: 197 926 2237 901 262 1553
Fragments arranged by size:
2237 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3867 493
Size: 3374 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6076
SphI G_CATG'C
Cuts at: 1132 3434 1132
Size: 2302 3774
SspI AAT'ATT
Cuts at: 5 52 4000 5
Size: 47 3948 2081
Fragments arranged by size:
3948 2081 47
StuI AGG'CCT
Cuts at: 1517 2285 3343 1517
Size: 768 1058 4250
Fragments arranged by size:
4250 1058 768
StyI C'CwwG_G
Cuts at: 513 1065 2004 2042 3205 3515 513
Size: 552 939 38 1163 310 3074
Fragments arranged by size:
3074 1163 939 552 310 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2132 2140 2341 3034 4103 4120 4273
Size: 1255 552 8 201 693 1069 17 153
Cuts at: 4273 4458 4903 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1069 693 552 445 201 185
153 17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4322 6034 96
Size: 274 80 33 51 1443 2345 1712 138
Fragments arranged by size:
2345 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2124 2156 2909 2941 6036 6068 2124
Size: 32 753 32 3095 32 2132
Fragments arranged by size:
3095 2132 753 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6076
XbaI T'CTAG_A
Cuts at: 3412 3412
Size: 6076
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6076
XmaI C'CCGG_G
Cuts at: 1870 3401 1870
Size: 1531 4545
XmnI GAAnn'nnTTC
Cuts at: 4205 4205
Size: 6076
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3884 276
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacI SanDI SapI
SrfI SwaI XcmI