Restriction map for
pFN21AE0785
(Circular) (Six-base) MAPSORT of: pFN21AE0785.seq Check: 6603 from: 1 to: 5764
pFN21AE0785 5764 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2098 2623 2098
Size: 525 5239
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 2871 3047 3085 2871
Size: 176 38 5550
Fragments arranged by size:
5550 176 38
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2266 2684 2890 3827 5211 5479 1632
Size: 634 418 206 937 1384 268 1917
Fragments arranged by size:
1917 1384 937 634 418 268 206
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2208 4663 5640 1203
Size: 1005 2455 977 1327
Fragments arranged by size:
2455 1327 1005 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 2622 1166
Size: 1456 4308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2480 1184
Size: 1296 4468
AlwNI CAG_nnn'CTG
Cuts at: 2485 2788 3507 5079 2485
Size: 303 719 1572 3170
Fragments arranged by size:
3170 1572 719 303
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 2558 3073 3299 3451 5560 1493
Size: 1065 515 226 152 2109 1697
Fragments arranged by size:
2109 1697 1065 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 2323 2537 2769 3089 1870
Size: 73 380 214 232 320 4545
Fragments arranged by size:
4545 380 320 232 214 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3039 3072 3039
Size: 33 5731
BamHI G'GATC_C
Cuts at: 3094 3461 3094
Size: 367 5397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2533 2859 2871 2899 3047
Size: 325 217 627 746 326 12 28 148
Cuts at: 3047 3085 4540 618
Size: 38 1455 1842
Fragments arranged by size:
1842 1455 746 627 326 325 217 148
38 28 12
BanII G_rGCy'C
Cuts at: 1391 2770 3083 1391
Size: 1379 313 4072
Fragments arranged by size:
4072 1379 313
BbeI G_GCGC'C
Cuts at: 2537 2537
Size: 5764
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4793
BbvCI CC'TCA_GC
Cuts at: 3039 3039
Size: 5764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2657 2691 3955 3989 1903
Size: 34 50 34 636 34 1264 34 3678
Fragments arranged by size:
3678 1264 636 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2594 4375 136
Size: 107 122 71 631 605 922 1781 1525
Fragments arranged by size:
1781 1525 922 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 2423 3181 2423
Size: 758 5006
Bme1580I G_kGCm'C
Cuts at: 1790 2904 3825 4981 1790
Size: 1114 921 1156 2573
Fragments arranged by size:
2573 1156 1114 921
BmgBI CAC'GTC
Cuts at: 1318 2209 1318
Size: 891 4873
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3495 4453 442
Size: 416 53 83 399 24 2078 958 1753
Fragments arranged by size:
2078 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 4424 1239
Size: 851 2334 2579
Fragments arranged by size:
2579 2334 851
Bpu10I CC'TnA_GC
Cuts at: 3039 3039
Size: 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 3889 4754 5052 2636
Size: 1253 865 298 3348
Fragments arranged by size:
3348 1253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3555 493
Size: 711 2351 2702
Fragments arranged by size:
2702 2351 711
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2534 3571 3953 4588
Size: 53 83 186 936 1001 1037 382 635
Cuts at: 4588 275
Size: 1451
Fragments arranged by size:
1451 1037 1001 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2896 4197 4595 4869 5016 5427 2896
Size: 1301 398 274 147 411 3233
Fragments arranged by size:
3233 1301 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2619 2649 1178
Size: 30 165 30 1216 30 4293
Fragments arranged by size:
4293 1216 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5764
BseYI C'CCAG_C
Cuts at: 2148 2171 2485 2519 2806 3032 4967 5463 2148
Size: 23 314 34 287 226 1935 496 2449
Fragments arranged by size:
2449 1935 496 314 287 226 34 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 2342 1163
Size: 573 390 216 4585
Fragments arranged by size:
4585 573 390 216
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2378 3214 3975 5003 1540
Size: 275 186 377 836 761 1028 2301
Fragments arranged by size:
2301 1028 836 761 377 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 3825 3910 4981 2220
Size: 1605 85 1071 3003
Fragments arranged by size:
3003 1605 1071 85
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2770 2904 3083 3825 3910 4981
Size: 399 430 550 134 179 742 85 1071
Cuts at: 4981 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 550 430 399 179 134
85
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2098 2623 2857 3035 3119 844
Size: 1254 525 234 178 84 3489
Fragments arranged by size:
3489 1254 525 234 178 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4259 4433 65
Size: 1183 3011 174 1396
Fragments arranged by size:
3011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2530 4408 4595 1700
Size: 110 720 1878 187 2869
Fragments arranged by size:
2869 1878 720 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 2334 2460 1925
Size: 409 126 5229
Fragments arranged by size:
5229 409 126
BssSI C'ACGA_G
Cuts at: 2393 3824 4836 2393
Size: 1431 1012 3321
Fragments arranged by size:
3321 1431 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2899 794
Size: 2105 3659
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstXI CCAn_nnnn'nTGG
Cuts at: 2836 2836
Size: 5764
BstYI r'GATC_y
Cuts at: 1972 3094 3461 3846 3863 5315 5401 5413 5759
Size: 1122 367 385 17 1452 86 12 346
Cuts at: 5759 1972
Size: 1977
Fragments arranged by size:
1977 1452 1122 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2202 2202
Size: 5764
BtgI C'CryG_G
Cuts at: 513 1065 2296 2583 3051 513
Size: 552 1231 287 468 3226
Fragments arranged by size:
3226 1231 552 468 287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2332 487
Size: 683 56 1106 3919
Fragments arranged by size:
3919 1106 683 56
BtsI GCAGTG_nn'
Cuts at: 3280 4074 4094 3280
Size: 794 20 4950
Fragments arranged by size:
4950 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DraIII CAC_nnn'GTG
Cuts at: 2832 3002 2832
Size: 170 5594
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2375 2586 2941
Size: 54 1425 50 275 18 545 211 355
Cuts at: 2941 2961 3211 4100 5729 8
Size: 20 250 889 1629 43
Fragments arranged by size:
1629 1425 889 545 355 275 250 211
54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2375 3211 1537
Size: 275 563 836 4090
Fragments arranged by size:
4090 836 563 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3694 1353
Size: 2341 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 2255 4351 4725 4871 387
Size: 1064 138 498 168 2096 374 146 1280
Fragments arranged by size:
2096 1280 1064 498 374 168 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2266 2684 2890 3827 4424 5211
Size: 393 458 176 418 206 937 597 787
Cuts at: 5211 5479 1239
Size: 268 1524
Fragments arranged by size:
1524 937 787 597 458 418 393 268
206 176
EcoNI CCTnn'n_nnAGG
Cuts at: 2105 2105
Size: 5764
EcoO109I rG'GnC_Cy
Cuts at: 2489 2596 2693 2846 2489
Size: 107 97 153 5407
Fragments arranged by size:
5407 153 107 97
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2069 2537 4911 1678
Size: 391 468 2374 2531
Fragments arranged by size:
2531 2374 468 391
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2032 2064 2960 2992 4405
Size: 32 135 32 460 32 896 32 1413
Cuts at: 4405 4437 4479 4511 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1413 896 460 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2431 3108 3354 669
Size: 988 281 493 677 246 3079
Fragments arranged by size:
3079 988 677 493 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KasI G'GCGC_C
Cuts at: 2533 2533
Size: 5764
KpnI G_GTAC'C
Cuts at: 2875 3051 3089 2875
Size: 176 38 5550
Fragments arranged by size:
5550 176 38
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4878 5062 1305
Size: 3573 184 2007
Fragments arranged by size:
3573 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2588 2963 10
Size: 54 1425 343 756 375 2811
Fragments arranged by size:
2811 1425 756 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2622 3722 4081 4240 518
Size: 648 1456 1100 359 159 2042
Fragments arranged by size:
2042 1456 1100 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2171 2233 2286 2298 2371 2389 2485 2579
Size: 565 62 53 12 73 18 96 94
Cuts at: 2579 2910 3180 3857 5005 5250 1606
Size: 331 270 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 331 270 245 96
94 73 62 53 18 12
NarI GG'CG_CC
Cuts at: 2534 2534
Size: 5764
NcoI C'CATG_G
Cuts at: 513 1065 2583 3051 513
Size: 552 1518 468 3226
Fragments arranged by size:
3226 1518 552 468
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 3122 4667 1132
Size: 97 1893 1545 2229
Fragments arranged by size:
2229 1893 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 1108
Size: 1137 4627
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2489 2693 2846 2489
Size: 204 153 5407
Fragments arranged by size:
5407 204 153
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PspXI vC'TCGA_Gb
Cuts at: 2323 2323
Size: 5764
PstI C_TGCA'G
Cuts at: 830 2481 2784 2994 3116 830
Size: 1651 303 210 122 3478
Fragments arranged by size:
3478 1651 303 210 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2171 2171
Size: 5764
RsrII CG'GwC_CG
Cuts at: 2528 2528
Size: 5764
SacII CC_GC'GG
Cuts at: 2299 2299
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SanDI GG'GwC_CC
Cuts at: 2489 2693 2489
Size: 204 5560
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SexAI A'CCwGG_T
Cuts at: 2874 2874
Size: 5764
SfcI C'TryA_G
Cuts at: 826 1045 2317 2477 2780 2990 3112 4247 4928
Size: 219 1272 160 303 210 122 1135 681
Cuts at: 4928 5119 826
Size: 191 1471
Fragments arranged by size:
1471 1272 1135 681 303 219 210 191
160 122
SfiI GGCCn_nnn'nGGCC
Cuts at: 2594 2594
Size: 5764
SfoI GGC'GCC
Cuts at: 2535 2535
Size: 5764
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 2539 3091 1872
Size: 667 552 4545
Fragments arranged by size:
4545 667 552
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2323 2651 3868 4769 5031 820
Size: 197 926 380 328 1217 901 262 1553
Fragments arranged by size:
1553 1217 926 901 380 328 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SrfI GCCC'GGGC
Cuts at: 2539 2539
Size: 5764
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5764
StyI C'CwwG_G
Cuts at: 513 1065 2583 2761 2964 3051 3203 513
Size: 552 1518 178 203 87 152 3074
Fragments arranged by size:
3074 1518 552 203 178 152 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 3791 3808 3961 4146 4591 325
Size: 1255 560 1651 17 153 185 445 1498
Fragments arranged by size:
1651 1498 1255 560 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4010 5722 96
Size: 274 80 33 51 1443 2033 1712 138
Fragments arranged by size:
2033 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2619 2651 5724 5756 2619
Size: 32 3073 32 2627
Fragments arranged by size:
3073 2627 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2145 2596 2145
Size: 451 5313
XhoI C'TCGA_G
Cuts at: 1943 2323 1943
Size: 380 5384
XmaI C'CCGG_G
Cuts at: 1870 2537 3089 1870
Size: 667 552 4545
Fragments arranged by size:
4545 667 552
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI
SacI SapI SwaI