Restriction map for
pFN21AE0779
(Circular) (Six-base) MAPSORT of: pFN21AE0779.seq Check: 5513 from: 1 to: 5749
pFN21AE0779 5749 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 2576 3812 5196 5464 1632
Size: 662 282 1236 1384 268 1917
Fragments arranged by size:
1917 1384 1236 662 282 268
AfeI AGC'GCT
Cuts at: 2953 2953
Size: 5749
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2161 3492 5064 2161
Size: 1331 1572 2846
Fragments arranged by size:
2846 1572 1331
ApaLI G'TGCA_C
Cuts at: 2483 3806 4962 2483
Size: 1323 1156 3270
Fragments arranged by size:
3270 1323 1156
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2681 2708 2862 3074 1870
Size: 73 738 27 154 212 4545
Fragments arranged by size:
4545 738 212 154 73 27
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2982 3070 4525 618
Size: 325 217 627 1195 88 1455 1842
Fragments arranged by size:
1842 1455 1195 627 325 217 88
BanII G_rGCy'C
Cuts at: 1391 2100 2187 2337 2421 2688 3068 1391
Size: 709 87 150 84 267 380 4072
Fragments arranged by size:
4072 709 380 267 150 87 84
BbeI G_GCGC'C
Cuts at: 2986 2986
Size: 5749
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4778
BbvCI CC'TCA_GC
Cuts at: 2874 2874
Size: 5749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2031 2065 2365 2399 3940
Size: 34 50 34 10 34 300 34 1541
Cuts at: 3940 3974 1903
Size: 34 3678
Fragments arranged by size:
3678 1541 300 50 34 34 34 34
34 10
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2504 2900 4360
Size: 107 122 71 631 605 832 396 1460
Cuts at: 4360 136
Size: 1525
Fragments arranged by size:
1525 1460 832 631 605 396 122 107
71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2487 3810 4966 1790
Size: 697 1323 1156 2573
Fragments arranged by size:
2573 1323 1156 697
BmgBI CAC'GTC
Cuts at: 1318 2298 1318
Size: 980 4769
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2150 2537 3010 4409 1239
Size: 845 66 387 473 1399 2579
Fragments arranged by size:
2579 1399 845 473 387 66
Bpu10I CC'TnA_GC
Cuts at: 2874 2874
Size: 5749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 2963 5743 2963
Size: 2780 2969
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2739 2754 2849 2983
Size: 53 83 186 936 1206 15 95 134
Cuts at: 2983 3556 3938 4573 275
Size: 573 382 635 1451
Fragments arranged by size:
1451 1206 936 635 573 382 186 134
95 83 53 15
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5524
Fragments arranged by size:
5524 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2081 2108 2762 2962 3050 1186
Size: 892 3 27 654 200 88 3885
Fragments arranged by size:
3885 892 654 200 88 27 3
BseYI C'CCAG_C
Cuts at: 2341 2499 2749 3041 4952 5448 2341
Size: 158 250 292 1911 496 2642
Fragments arranged by size:
2642 1911 496 292 250 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2904 3199 3960 4988 1540
Size: 275 186 903 295 761 1028 2301
Fragments arranged by size:
2301 1028 903 761 295 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2155 2187 2487 2688 2976 3810 3895 4966
Size: 55 32 300 201 288 834 85 1071
Cuts at: 4966 2100
Size: 2883
Fragments arranged by size:
2883 1071 834 300 288 201 85 55
32
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2155 2187 2337 2421 2487 2688
Size: 399 310 55 32 150 84 66 201
Cuts at: 2688 2976 3068 3810 3895 4966 1391
Size: 288 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 310 288 201 150
92 85 84 66 55 32
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3037 3104 844
Size: 2193 67 3489
Fragments arranged by size:
3489 2193 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2704 3026 3636 1394
Size: 429 881 322 610 3507
Fragments arranged by size:
3507 881 610 429 322
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 2592 2796 4393 4580 1700
Size: 110 222 560 204 1597 187 2869
Fragments arranged by size:
2869 1597 560 222 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 2124 2346 2691 1925
Size: 199 222 345 4983
Fragments arranged by size:
4983 345 222 199
BssSI C'ACGA_G
Cuts at: 2623 3809 4821 2623
Size: 1186 1012 3551
Fragments arranged by size:
3551 1186 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 2239 1184
Size: 1055 4694
BstYI r'GATC_y
Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744
Size: 1107 367 385 17 1452 86 12 346
Cuts at: 5744 1972
Size: 1977
Fragments arranged by size:
1977 1452 1107 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2305 2478 2533 2898 513
Size: 552 1240 173 55 365 3364
Fragments arranged by size:
3364 1240 552 365 173 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 3024 3265 4059 4079 3024
Size: 241 794 20 4694
Fragments arranged by size:
4694 794 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2224 2617 2652
Size: 54 1425 50 275 18 394 393 35
Cuts at: 2652 2805 2901 3196 4085 5714 8
Size: 153 96 295 889 1629 43
Fragments arranged by size:
1629 1425 889 394 393 295 275 153
96 54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2901 3196 1537
Size: 275 1089 295 4090
Fragments arranged by size:
4090 1089 295 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2768 3027 3679 1353
Size: 1415 259 652 3423
Fragments arranged by size:
3423 1415 652 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 2500 3026 4336 4710 4856
Size: 1064 138 462 449 526 1310 374 146
Cuts at: 4856 387
Size: 1280
Fragments arranged by size:
1310 1280 1064 526 462 449 374 146
138
EcoICRI GAG'CTC
Cuts at: 2098 2185 2686 2098
Size: 87 501 5161
Fragments arranged by size:
5161 501 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2150 2294 2537 2576 3010 3812
Size: 393 452 66 144 243 39 434 802
Cuts at: 3812 4409 5196 5464 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 802 787 597 452 434 393 268
243 144 66 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2210 2807 2210
Size: 597 5152
EcoO109I rG'GnC_Cy
Cuts at: 2434 2551 2713 2434
Size: 117 162 5470
Fragments arranged by size:
5470 162 117
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2599 2750 2955 2986 4896 1678
Size: 921 151 205 31 1910 2531
Fragments arranged by size:
2531 1910 921 205 151 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4390 4422 4464 4496 1373
Size: 32 135 32 2818 32 42 32 2626
Fragments arranged by size:
2818 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3093 3339 669
Size: 988 281 1155 246 3079
Fragments arranged by size:
3079 1155 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KasI G'GCGC_C
Cuts at: 2982 2982
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4863 5047 1305
Size: 3558 184 2007
Fragments arranged by size:
3558 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3927
Fragments arranged by size:
3927 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3707 4066 4225 518
Size: 648 2541 359 159 2042
Fragments arranged by size:
2541 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2480 2490 2503 2535 2900 2998 3165 3842
Size: 874 10 13 32 365 98 167 677
Cuts at: 3842 4990 5235 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 874 677 365 245 167 98
32 13 10
NaeI GCC'GGC
Cuts at: 2034 2594 2034
Size: 560 5189
NarI GG'CG_CC
Cuts at: 2983 2983
Size: 5749
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NgoMIV G'CCGG_C
Cuts at: 2032 2592 2032
Size: 560 5189
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 3107 4652 1132
Size: 97 1878 1545 2229
Fragments arranged by size:
2229 1878 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2206 2790 1348
Size: 858 584 4307
Fragments arranged by size:
4307 858 584
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2434 2551 2434
Size: 117 5632
PshAI GACnn'nnGTC
Cuts at: 1613 2162 1613
Size: 549 5200
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PstI C_TGCA'G
Cuts at: 830 3101 830
Size: 2271 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2503 2503
Size: 5749
SacI G_AGCT'C
Cuts at: 2100 2187 2688 2100
Size: 87 501 5161
Fragments arranged by size:
5161 501 87
SacII CC_GC'GG
Cuts at: 2481 2536 2901 2481
Size: 55 365 5329
Fragments arranged by size:
5329 365 55
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SapI GCTCTTCn'nnn_
Cuts at: 2768 2768
Size: 5749
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2401 3097 4232 4913 5104 826
Size: 219 1356 696 1135 681 191 1471
Fragments arranged by size:
1471 1356 1135 696 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2900 2900
Size: 5749
SfoI GGC'GCC
Cuts at: 2984 2984
Size: 5749
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 2864 3076 1872
Size: 992 212 4545
Fragments arranged by size:
4545 992 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 2629 1517
Size: 1112 4637
StyI C'CwwG_G
Cuts at: 513 1065 2457 3188 513
Size: 552 1392 731 3074
Fragments arranged by size:
3074 1392 731 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 2624 3776 3793 3946 4131 4576
Size: 1255 678 366 1152 17 153 185 445
Cuts at: 4576 325
Size: 1498
Fragments arranged by size:
1498 1255 1152 678 445 366 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 3995 5707
Size: 274 80 33 51 1443 543 1475 1712
Cuts at: 5707 96
Size: 138
Fragments arranged by size:
1712 1475 1443 543 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2219 2219
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 2862 3074 1870
Size: 992 212 4545
Fragments arranged by size:
4545 992 212
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SanDI SexAI SrfI SwaI