Restriction map for
pFN21AE0773
(Circular) (Six-base) MAPSORT of: pFN21AE0773.seq Check: 4802 from: 1 to: 5737
pFN21AE0773 5737 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2406 2406
Size: 5737
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3080 1937
Size: 1143 4594
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2666 3800 5184 5452 1632
Size: 1034 1134 1384 268 1917
Fragments arranged by size:
1917 1384 1134 1034 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 2274 4636 5613 1203
Size: 1071 2362 977 1327
Fragments arranged by size:
2362 1327 1071 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 4466 4466
Size: 5737
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2801 1184
Size: 1617 4120
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 5705
AlwNI CAG_nnn'CTG
Cuts at: 3480 5052 3480
Size: 1572 4165
ApaLI G'TGCA_C
Cuts at: 3794 4950 3794
Size: 1156 4581
ApoI r'AATT_y
Cuts at: 1493 3046 3272 3424 5533 1493
Size: 1553 226 152 2109 1697
Fragments arranged by size:
2109 1697 1553 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 3062 1870
Size: 73 343 776 4545
Fragments arranged by size:
4545 776 343 73
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2708 3058 4513 618
Size: 325 217 627 921 350 1455 1842
Fragments arranged by size:
1842 1455 921 627 350 325 217
BanII G_rGCy'C
Cuts at: 1391 2490 2634 2905 3056 1391
Size: 1099 144 271 151 4072
Fragments arranged by size:
4072 1099 271 151 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2702 928
Size: 971 803 3963
Fragments arranged by size:
3963 971 803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2362 2396 3928 3962 1903
Size: 34 425 34 1532 34 3678
Fragments arranged by size:
3678 1532 425 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3629 4839 3629
Size: 1210 4527
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4348 136
Size: 107 122 71 631 605 2676 1525
Fragments arranged by size:
2676 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5732 5732
Size: 5737
BlpI GC'TnA_GC
Cuts at: 3154 3154
Size: 5737
Bme1580I G_kGCm'C
Cuts at: 1790 2895 3798 4954 1790
Size: 1105 903 1156 2573
Fragments arranged by size:
2573 1156 1105 903
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5737
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4426 442
Size: 416 53 83 399 24 2051 958 1753
Fragments arranged by size:
2051 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4397 1239
Size: 3158 2579
Bpu10I CC'TnA_GC
Cuts at: 2066 2927 2066
Size: 861 4876
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2303 2618 3862 4727 5025 2303
Size: 315 1244 865 298 3015
Fragments arranged by size:
3015 1244 865 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2837 2971 4400 882
Size: 617 1338 134 1429 2219
Fragments arranged by size:
2219 1429 1338 617 134
BsaAI yAC'GTr
Cuts at: 493 1204 3528 493
Size: 711 2324 2702
Fragments arranged by size:
2702 2324 711
BsaBI GATnn'nnATC
Cuts at: 2384 5731 2384
Size: 3347 2390
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2245 3544 3926 4561
Size: 53 83 186 936 712 1299 382 635
Cuts at: 4561 275
Size: 1451
Fragments arranged by size:
1451 1299 936 712 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2091 2442 4170 4568 4842 4989 5400 2091
Size: 351 1728 398 274 147 411 2428
Fragments arranged by size:
2428 1728 411 398 351 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 1178
Size: 30 165 30 1497 30 3985
Fragments arranged by size:
3985 1497 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2461 2564 2704 1186
Size: 1275 103 140 4219
Fragments arranged by size:
4219 1275 140 103
BseYI C'CCAG_C
Cuts at: 2079 4940 5436 2079
Size: 2861 496 2380
Fragments arranged by size:
2861 2380 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2100 2384 2497 1163
Size: 573 364 284 113 4403
Fragments arranged by size:
4403 573 364 284 113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2044 3187 3948 4976 1540
Size: 275 186 43 1143 761 1028 2301
Fragments arranged by size:
2301 1143 1028 761 275 186 43
BsiHKAI G_wGCw'C
Cuts at: 2072 2490 3798 3883 4954 2072
Size: 418 1308 85 1071 2855
Fragments arranged by size:
2855 1308 1071 418 85
BsmI GAATG_Cn'
Cuts at: 3248 3341 3248
Size: 93 5644
BsmBI CGTCTCn'nnnn_
Cuts at: 2426 2426
Size: 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2490 2634 2895 2905 3056 3798
Size: 399 282 418 144 261 10 151 742
Cuts at: 3798 3883 4954 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 418 399 282 261 151
144 85 10
BspEI T'CCGG_A
Cuts at: 2091 5400 2091
Size: 3309 2428
BspHI T'CATG_A
Cuts at: 2127 3626 5356 2127
Size: 1499 1730 2508
Fragments arranged by size:
2508 1730 1499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2406 2730 3092 844
Size: 1562 324 362 3489
Fragments arranged by size:
3489 1562 362 324
BsrBI CCG'CTC
Cuts at: 1394 1823 2136 3624 1394
Size: 429 313 1488 3507
Fragments arranged by size:
3507 1488 429 313
BsrDI GCAATG_nn'
Cuts at: 65 1248 4232 4406 65
Size: 1183 2984 174 1396
Fragments arranged by size:
2984 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4381 4568 1700
Size: 110 2571 187 2869
Fragments arranged by size:
2869 2571 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2368 794
Size: 1574 4163
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstXI CCAn_nnnn'nTGG
Cuts at: 2545 2545
Size: 5737
BstYI r'GATC_y
Cuts at: 1972 2451 2767 3067 3434 3819 3836 5288 5374
Size: 479 316 300 367 385 17 1452 86
Cuts at: 5374 5386 5732 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 479 385 367 346 316 300
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2687 2687
Size: 5737
BtgI C'CryG_G
Cuts at: 513 1065 2004 2479 2578 2895 513
Size: 552 939 475 99 317 3355
Fragments arranged by size:
3355 939 552 475 317 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4998
Fragments arranged by size:
4998 683 56
BtsI GCAGTG_nn'
Cuts at: 2194 3253 4047 4067 2194
Size: 1059 794 20 3864
Fragments arranged by size:
3864 1059 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 3041 3041
Size: 5737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4744 809
Size: 507 3428 1802
Fragments arranged by size:
3428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2164 3184
Size: 54 1425 50 275 18 211 123 1020
Cuts at: 3184 4073 5702 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1020 889 275 211 123 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2041 3184 1537
Size: 275 229 1143 4090
Fragments arranged by size:
4090 1143 275 229
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 2501 3667 1353
Size: 980 168 1166 3423
Fragments arranged by size:
3423 1166 980 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2488 2488
Size: 5737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2666 3800 4397 5184 5452 1239
Size: 393 1034 1134 597 787 268 1524
Fragments arranged by size:
1524 1134 1034 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2686 2960 2686
Size: 274 5463
EcoO109I rG'GnC_Cy
Cuts at: 2323 2908 2323
Size: 585 5152
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 5737
FspI TGC'GCA
Cuts at: 2372 4243 2372
Size: 1871 3866
HaeII r_GCGC'y
Cuts at: 1678 2318 4884 1678
Size: 640 2566 2531
Fragments arranged by size:
2566 2531 640
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2230 2262 2816 2848 2900
Size: 32 135 32 658 32 554 32 52
Cuts at: 2900 2932 4378 4410 4452 4484 1373
Size: 32 1446 32 42 32 2626
Fragments arranged by size:
2626 1446 658 554 135 52 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3327 669
Size: 988 281 1143 246 3079
Fragments arranged by size:
3079 1143 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5737
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 2223 3336 2223
Size: 1113 4624
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4851 5035 1305
Size: 3546 184 2007
Fragments arranged by size:
3546 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2166 10
Size: 54 1425 343 334 3581
Fragments arranged by size:
3581 1425 343 334 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 2783 3695 4054 4213 518
Size: 648 1377 240 912 359 159 2042
Fragments arranged by size:
2042 1377 912 648 359 240 159
MspA1I CmG'CkG
Cuts at: 1606 2156 2368 2481 3153 3830 4978 5223 1606
Size: 550 212 113 672 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 550 245 212 113
NcoI C'CATG_G
Cuts at: 513 1065 2004 2578 513
Size: 552 939 574 3672
Fragments arranged by size:
3672 939 574 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 2678 2752 3095 4640 1132
Size: 97 1449 74 343 1545 2229
Fragments arranged by size:
2229 1545 1449 343 97 74
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5737
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5737
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2323 2908 2323
Size: 585 5152
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 3307 3307
Size: 5737
PstI C_TGCA'G
Cuts at: 830 2505 3089 830
Size: 1675 584 3478
Fragments arranged by size:
3478 1675 584
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
PvuII CAG'CTG
Cuts at: 2156 2368 2156
Size: 212 5525
SacI G_AGCT'C
Cuts at: 2490 2490
Size: 5737
SacII CC_GC'GG
Cuts at: 2482 2482
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SapI GCTCTTCn'nnn_
Cuts at: 2501 2501
Size: 5737
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2501 3085 4220 4901 5092 826
Size: 219 1456 584 1135 681 191 1471
Fragments arranged by size:
1471 1456 1135 681 584 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 3064 1872
Size: 1192 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 2633 3841 4742 5004 820
Size: 197 926 375 315 1208 901 262 1553
Fragments arranged by size:
1553 1208 926 901 375 315 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 2678 2752 3095 1132
Size: 1546 74 343 3774
Fragments arranged by size:
3774 1546 343 74
SspI AAT'ATT
Cuts at: 5 52 3661 5
Size: 47 3609 2081
Fragments arranged by size:
3609 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 2004 2578 2978 3176 513
Size: 552 939 574 400 198 3074
Fragments arranged by size:
3074 939 574 552 400 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3764 3781 3934 4119 4564 325
Size: 1255 2184 17 153 185 445 1498
Fragments arranged by size:
2184 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 2571 3983
Size: 274 80 33 51 1443 300 294 1412
Cuts at: 3983 5695 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1412 300 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2759 2791 5697 5729 2759
Size: 32 2906 32 2767
Fragments arranged by size:
2906 2767 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5737
XbaI T'CTAG_A
Cuts at: 2464 3073 2464
Size: 609 5128
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 3062 1870
Size: 1192 4545
XmnI GAAnn'nnTTC
Cuts at: 3866 3866
Size: 5737
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI