Restriction map for
pFN21AE0765
(Circular) (Six-base) MAPSORT of: pFN21AE0765.seq Check: 261 from: 1 to: 5722
pFN21AE0765 5722 bp
With 182 enzymes: SgfI *
March 28, 2012 12:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 1937
Size: 1128 4594
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2529 2969 3785 5169 5437 1632
Size: 897 440 816 1384 268 1917
Fragments arranged by size:
1917 1384 897 816 440 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 2527 4621 5598 1203
Size: 1324 2094 977 1327
Fragments arranged by size:
2094 1327 1324 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AlwNI CAG_nnn'CTG
Cuts at: 3465 5037 3465
Size: 1572 4150
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 2295 2724 2970 3031 3257 3409 5518 1493
Size: 802 429 246 61 226 152 2109 1697
Fragments arranged by size:
2109 1697 802 429 246 226 152 61
AseI AT'TA_AT
Cuts at: 160 1032 2031 2672 4276 160
Size: 872 999 641 1604 1606
Fragments arranged by size:
1606 1604 999 872 641
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 3047 1870
Size: 73 1104 4545
Fragments arranged by size:
4545 1104 73
BamHI G'GATC_C
Cuts at: 3052 3419 3052
Size: 367 5355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2796 3043 4498 618
Size: 325 217 627 1009 247 1455 1842
Fragments arranged by size:
1842 1455 1009 627 325 247 217
BanII G_rGCy'C
Cuts at: 1391 2327 3041 1391
Size: 936 714 4072
Fragments arranged by size:
4072 936 714
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2278 2548 2762 928
Size: 971 379 270 214 3888
Fragments arranged by size:
3888 971 379 270 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3614 4824 3614
Size: 1210 4512
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5722
BfrBI ATG'CAT
Cuts at: 2918 2918
Size: 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4333 136
Size: 107 122 71 631 605 2661 1525
Fragments arranged by size:
2661 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2252 5717 2252
Size: 3465 2257
BlpI GC'TnA_GC
Cuts at: 2259 2321 2326 3139 2259
Size: 62 5 813 4842
Fragments arranged by size:
4842 813 62 5
Bme1580I G_kGCm'C
Cuts at: 1790 2801 3783 4939 1790
Size: 1011 982 1156 2573
Fragments arranged by size:
2573 1156 1011 982
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3453 4411 442
Size: 416 53 83 399 24 2036 958 1753
Fragments arranged by size:
2036 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2507 4382 1239
Size: 1268 1875 2579
Fragments arranged by size:
2579 1875 1268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3847 4712 5010 3847
Size: 865 298 4559
Fragments arranged by size:
4559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4385 882
Size: 617 2886 2219
Fragments arranged by size:
2886 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 2411 2685 5716 2411
Size: 274 3031 2417
Fragments arranged by size:
3031 2417 274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3529 3911 4546 275
Size: 53 83 186 936 1996 382 635 1451
Fragments arranged by size:
1996 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4155 4553 4827 4974 5385 4155
Size: 398 274 147 411 4492
Fragments arranged by size:
4492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5497
Fragments arranged by size:
5497 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2556 1186
Size: 1370 4352
BseYI C'CCAG_C
Cuts at: 4925 5421 4925
Size: 496 5226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3172 3933 4961 1540
Size: 275 186 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2265 2327 3783 3868 4939 2100
Size: 165 62 1456 85 1071 2883
Fragments arranged by size:
2883 1456 1071 165 85 62
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2265 2327 2801 3041 3783 3868
Size: 399 310 165 62 474 240 742 85
Cuts at: 3868 4939 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 310 240 165
85 62
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 3611 5341 3611
Size: 1730 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3077 844
Size: 2233 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3609 1394
Size: 429 1786 3507
Fragments arranged by size:
3507 1786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4217 4391 65
Size: 1183 2969 174 1396
Fragments arranged by size:
2969 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4366 4553 1700
Size: 110 2556 187 2869
Fragments arranged by size:
2869 2556 187 110
BsrGI T'GTAC_A
Cuts at: 96 2082 2813 96
Size: 1986 731 3005
Fragments arranged by size:
3005 1986 731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 3782 4794 3782
Size: 1012 4710
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstXI CCAn_nnnn'nTGG
Cuts at: 2181 2181
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2252 2612 3052 3419 3804 3821 5273 5359
Size: 280 360 440 367 385 17 1452 86
Cuts at: 5359 5371 5717 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 440 385 367 360 346 280
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2196 513
Size: 552 1131 4039
Fragments arranged by size:
4039 1131 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4983
Fragments arranged by size:
4983 683 56
BtsI GCAGTG_nn'
Cuts at: 2533 3238 4032 4052 2533
Size: 705 794 20 4203
Fragments arranged by size:
4203 794 705 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2101 2136 2838 2873 529
Size: 35 1537 35 702 35 3378
Fragments arranged by size:
3378 1537 702 35 35 35
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 3169 4058
Size: 54 1425 50 275 18 369 970 889
Cuts at: 4058 5687 8
Size: 1629 43
Fragments arranged by size:
1629 1425 970 889 369 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 3652 1353
Size: 1152 1147 3423
Fragments arranged by size:
3423 1152 1147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4309 4683 4829 387
Size: 1064 138 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2325 2325
Size: 5722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2507 2529 2969 3785 4382 5169 5437
Size: 393 875 22 440 816 597 787 268
Cuts at: 5437 1239
Size: 1524
Fragments arranged by size:
1524 875 816 787 597 440 393 268
22
EcoRI G'AATT_C
Cuts at: 3031 3409 3031
Size: 378 5344
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 2508 4869 1678
Size: 830 2361 2531
Fragments arranged by size:
2531 2361 830
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4363 4395 4437 4469 1373
Size: 32 135 32 2791 32 42 32 2626
Fragments arranged by size:
2791 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 3312 669
Size: 988 281 1128 246 3079
Fragments arranged by size:
3079 1128 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5722
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2426 4836 5020 1305
Size: 1121 2410 184 2007
Fragments arranged by size:
2410 2007 1121 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 10
Size: 54 1425 343 369 3531
Fragments arranged by size:
3531 1425 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2992 3680 4039 4198 518
Size: 648 1826 688 359 159 2042
Fragments arranged by size:
2042 1826 688 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3138 3815 4963 5208 1606
Size: 1532 677 1148 245 2120
Fragments arranged by size:
2120 1532 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2196 513
Size: 552 1131 4039
Fragments arranged by size:
4039 1131 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5722
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NsiI A_TGCA'T
Cuts at: 2920 2920
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 2531 3080 4625 1132
Size: 97 1302 549 1545 2229
Fragments arranged by size:
2229 1545 1302 549 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 2527 4621 2527
Size: 2094 3628
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5722
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PstI C_TGCA'G
Cuts at: 830 2540 3074 830
Size: 1710 534 3478
Fragments arranged by size:
3478 1710 534
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
SacI G_AGCT'C
Cuts at: 2327 2327
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 2536 3070 4205 4886 5077 826
Size: 219 1491 534 1135 681 191 1471
Fragments arranged by size:
1491 1471 1135 681 534 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3826 4727 4989 820
Size: 197 926 1883 901 262 1553
Fragments arranged by size:
1883 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 2035 3646 5
Size: 47 1983 1611 2081
Fragments arranged by size:
2081 1983 1611 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5722
StyI C'CwwG_G
Cuts at: 513 1065 2196 2930 3161 513
Size: 552 1131 734 231 3074
Fragments arranged by size:
3074 1131 734 552 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3749 3766 3919 4104 4549 325
Size: 1255 2169 17 153 185 445 1498
Fragments arranged by size:
2169 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 2813 3968
Size: 274 80 33 51 1443 105 731 1155
Cuts at: 3968 5680 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1155 731 274 138 105 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5682 5714 5682
Size: 32 5690
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2203
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5722
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 3851 3851
Size: 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI