Restriction map for
pFN21AE0764
(Circular) (Six-base) MAPSORT of: pFN21AE0764.seq Check: 403 from: 1 to: 6016
pFN21AE0764 6016 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3359 1937
Size: 1422 4594
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 2720 4143 4516 1563
Size: 1157 1423 373 3063
Fragments arranged by size:
3063 1423 1157 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 2718 2949 4079 5463 5731 1632
Size: 599 487 231 1130 1384 268 1917
Fragments arranged by size:
1917 1384 1130 599 487 268 231
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 2391 4915 5892 1203
Size: 1188 2524 977 1327
Fragments arranged by size:
2524 1327 1188 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2323 1184
Size: 1139 4877
AlwNI CAG_nnn'CTG
Cuts at: 3142 3759 5331 3142
Size: 617 1572 3827
Fragments arranged by size:
3827 1572 617
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 3307 3325 3551 3703 5812 1493
Size: 1814 18 226 152 2109 1697
Fragments arranged by size:
2109 1814 1697 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 3341 1870
Size: 73 1398 4545
Fragments arranged by size:
4545 1398 73
BamHI G'GATC_C
Cuts at: 3346 3713 3346
Size: 367 5649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3337 4792 618
Size: 325 217 627 1550 1455 1842
Fragments arranged by size:
1842 1550 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3335 1391
Size: 1944 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2624 928
Size: 971 725 4320
Fragments arranged by size:
4320 971 725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4207 4241 1903
Size: 34 2270 34 3678
Fragments arranged by size:
3678 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3908 5118 3908
Size: 1210 4806
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4627 136
Size: 107 122 71 631 605 2955 1525
Fragments arranged by size:
2955 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2116 6011 2116
Size: 3895 2121
BlpI GC'TnA_GC
Cuts at: 3433 3433
Size: 6016
Bme1580I G_kGCm'C
Cuts at: 1790 4077 5233 1790
Size: 2287 1156 2573
Fragments arranged by size:
2573 2287 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6016
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3747 4705 442
Size: 416 53 83 399 24 2330 958 1753
Fragments arranged by size:
2330 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2088 2088
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4676 1239
Size: 3437 2579
Bpu10I CC'TnA_GC
Cuts at: 2177 2177
Size: 6016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2058 3260 4141 5006 5304 2058
Size: 1202 881 865 298 2770
Fragments arranged by size:
2770 1202 881 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4679 882
Size: 617 3180 2219
Fragments arranged by size:
3180 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3807 493
Size: 711 2603 2702
Fragments arranged by size:
2702 2603 711
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4205 4840 275
Size: 53 83 186 936 2290 382 635 1451
Fragments arranged by size:
2290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2936 2966 1178
Size: 30 165 30 1533 30 4228
Fragments arranged by size:
4228 1533 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2113 2502 2973 1186
Size: 927 389 471 4229
Fragments arranged by size:
4229 927 471 389
BseYI C'CCAG_C
Cuts at: 2567 2624 5219 5715 2567
Size: 57 2595 496 2868
Fragments arranged by size:
2868 2595 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2399 2526 1163
Size: 573 663 127 4653
Fragments arranged by size:
4653 663 573 127
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2038 4077 4162 5233 2038
Size: 2039 85 1071 2821
Fragments arranged by size:
2821 2039 1071 85
BsmI GAATG_Cn'
Cuts at: 2312 3527 3620 2312
Size: 1215 93 4708
Fragments arranged by size:
4708 1215 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 3335 4077 4162 5233 1391
Size: 399 248 1297 742 85 1071 2174
Fragments arranged by size:
2174 1297 1071 742 399 248 85
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 2857 3189 3905 5635 2857
Size: 332 716 1730 3238
Fragments arranged by size:
3238 1730 716 332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2283 3371 844
Size: 1439 1088 3489
Fragments arranged by size:
3489 1439 1088
BsrBI CCG'CTC
Cuts at: 1394 1823 3903 1394
Size: 429 2080 3507
Fragments arranged by size:
3507 2080 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4511 4685 65
Size: 1183 3263 174 1396
Fragments arranged by size:
3263 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4660 4847 1700
Size: 110 2850 187 2869
Fragments arranged by size:
2869 2850 187 110
BsrGI T'GTAC_A
Cuts at: 96 2952 96
Size: 2856 3160
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6016
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 2116 2479 3346 3713 4098 4115 5567 5653
Size: 144 363 867 367 385 17 1452 86
Cuts at: 5653 5665 6011 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 867 385 367 363 346 144
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5464
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5277
Fragments arranged by size:
5277 683 56
BtsI GCAGTG_nn'
Cuts at: 2038 3532 4326 4346 2038
Size: 1494 794 20 3708
Fragments arranged by size:
3708 1494 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2923 5023 809
Size: 507 1607 2100 1802
Fragments arranged by size:
2100 1802 1607 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3123 3463 4352
Size: 54 1425 50 275 18 1293 340 889
Cuts at: 4352 5981 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1293 889 340 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2602 2907 3946 1353
Size: 1249 305 1039 3423
Fragments arranged by size:
3423 1249 1039 305
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4603 4977 5123 387
Size: 1064 138 3014 374 146 1280
Fragments arranged by size:
3014 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2718 2949 4079 4676 5463 5731
Size: 393 599 487 231 1130 597 787 268
Cuts at: 5731 1239
Size: 1524
Fragments arranged by size:
1524 1130 787 599 597 487 393 268
231
EcoO109I rG'GnC_Cy
Cuts at: 2773 2773
Size: 6016
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
EcoRV GAT'ATC
Cuts at: 2106 2106
Size: 6016
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 5163 1678
Size: 3485 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2323 2355 2645 2677 2839
Size: 32 135 32 751 32 290 32 162
Cuts at: 2839 2871 2883 2915 4657 4689 4731 4763 1373
Size: 32 12 32 1742 32 42 32 2626
Fragments arranged by size:
2626 1742 751 290 162 135 42 32
32 32 32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 2680 3360 3606 669
Size: 988 281 334 408 680 246 3079
Fragments arranged by size:
3079 988 680 408 334 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 2680 3606 2680
Size: 926 5090
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2075 5130 5314 1305
Size: 770 3055 184 2007
Fragments arranged by size:
3055 2007 770 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3125 10
Size: 54 1425 343 1293 2901
Fragments arranged by size:
2901 1425 1293 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2305 3103 3974 4333 4492 518
Size: 648 1139 798 871 359 159 2042
Fragments arranged by size:
2042 1139 871 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2369 3229 3432 4109 5257 5502 1606
Size: 763 860 203 677 1148 245 2120
Fragments arranged by size:
2120 1148 860 763 677 245 203
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5464
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 2324 3374 4919 1132
Size: 97 1095 1050 1545 2229
Fragments arranged by size:
2229 1545 1095 1050 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3142 1108
Size: 2034 3982
PfoI T'CCnGG_A
Cuts at: 3207 3207
Size: 6016
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PstI C_TGCA'G
Cuts at: 830 3368 830
Size: 2538 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
PvuII CAG'CTG
Cuts at: 2369 2369
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 3364 4499 5180 5371 826
Size: 219 2319 1135 681 191 1471
Fragments arranged by size:
2319 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2073 3275 4120 5021 5283 820
Size: 197 926 130 1202 845 901 262 1553
Fragments arranged by size:
1553 1202 926 901 845 262 197 130
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 2324 3374 1132
Size: 1192 1050 3774
Fragments arranged by size:
3774 1192 1050
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6016
StyI C'CwwG_G
Cuts at: 513 1065 2137 3455 513
Size: 552 1072 1318 3074
Fragments arranged by size:
3074 1318 1072 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 2581 2638 3009 4043 4060 4213
Size: 1255 611 390 57 371 1034 17 153
Cuts at: 4213 4398 4843 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1034 611 445 390 371 185
153 57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2952 4262
Size: 274 80 33 51 1443 249 726 1310
Cuts at: 4262 5974 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1310 726 274 249 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5976 6008 5976
Size: 32 5984
Tth111I GACn'n_nGTC
Cuts at: 1316 2530 1316
Size: 1214 4802
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 2195 4145 2195
Size: 1950 4066
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI