Restriction map for
pFN21AE0760
(Circular) (Six-base) MAPSORT of: pFN21AE0760.seq Check: 3299 from: 1 to: 6001
pFN21AE0760 6001 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2408 3344 1937
Size: 471 936 4594
Fragments arranged by size:
4594 936 471
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4064 5448 5716 1632
Size: 2432 1384 268 1917
Fragments arranged by size:
2432 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 2403 4900 5877 1203
Size: 1200 2497 977 1327
Fragments arranged by size:
2497 1327 1200 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 2709 2793 3213 1166
Size: 1543 84 420 3954
Fragments arranged by size:
3954 1543 420 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6001
AlwNI CAG_nnn'CTG
Cuts at: 3744 5316 3744
Size: 1572 4429
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 2176 2203 2532 2784 2868 3036 3204 3288
Size: 683 27 329 252 84 168 168 84
Cuts at: 3288 3310 3536 3688 5797 1493
Size: 22 226 152 2109 1697
Fragments arranged by size:
2109 1697 683 329 252 226 168 168
152 84 84 27 22
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3326 1870
Size: 73 1383 4545
Fragments arranged by size:
4545 1383 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3322 4777 618
Size: 325 217 627 1535 1455 1842
Fragments arranged by size:
1842 1535 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 3098 3320 1391
Size: 892 815 222 4072
Fragments arranged by size:
4072 892 815 222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2406 928
Size: 971 507 4523
Fragments arranged by size:
4523 971 507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4192 4226 1903
Size: 34 50 34 2171 34 3678
Fragments arranged by size:
3678 2171 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2104 3893 5103 2104
Size: 1789 1210 3002
Fragments arranged by size:
3002 1789 1210
BclI T'GATC_A
Cuts at: 1548 2167 2773 1548
Size: 619 606 4776
Fragments arranged by size:
4776 619 606
BfrBI ATG'CAT
Cuts at: 2071 2956 2071
Size: 885 5116
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4612 136
Size: 107 122 71 631 605 2940 1525
Fragments arranged by size:
2940 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2323 5996 2323
Size: 3673 2328
BlpI GC'TnA_GC
Cuts at: 3418 3418
Size: 6001
Bme1580I G_kGCm'C
Cuts at: 1790 4062 5218 1790
Size: 2272 1156 2573
Fragments arranged by size:
2573 2272 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3732 4690 442
Size: 416 53 83 399 24 2315 958 1753
Fragments arranged by size:
2315 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4661 1239
Size: 3422 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2124 2914 4126 4991 5289 2124
Size: 790 1212 865 298 2836
Fragments arranged by size:
2836 1212 865 790 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2336 4664 882
Size: 617 837 2328 2219
Fragments arranged by size:
2328 2219 837 617
BsaAI yAC'GTr
Cuts at: 493 1204 3792 493
Size: 711 2588 2702
Fragments arranged by size:
2702 2588 711
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3808 4190 4825 275
Size: 53 83 186 936 2275 382 635 1451
Fragments arranged by size:
2275 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4434 4832 5106 5253 5664 4434
Size: 398 274 147 411 4771
Fragments arranged by size:
4771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5776
Fragments arranged by size:
5776 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2007 2072 1186
Size: 821 65 5115
Fragments arranged by size:
5115 821 65
BseYI C'CCAG_C
Cuts at: 5204 5700 5204
Size: 496 5505
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5428
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4212 5240 1540
Size: 275 186 1450 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3098 4062 4147 5218 3098
Size: 964 85 1071 3881
Fragments arranged by size:
3881 1071 964 85
BsmI GAATG_Cn'
Cuts at: 2073 2159 2958 3512 3605 2073
Size: 86 799 554 93 4469
Fragments arranged by size:
4469 799 554 93 86
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 3098 3320 4062 4147 5218 1391
Size: 399 493 815 222 742 85 1071 2174
Fragments arranged by size:
2174 1071 815 742 493 399 222 85
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3356 844
Size: 2512 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4496 4670 65
Size: 1183 3248 174 1396
Fragments arranged by size:
3248 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2104 4645 4832 1700
Size: 110 294 2541 187 2869
Fragments arranged by size:
2869 2541 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6001
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 2323 3331 3698 4083 4100 5552 5638 5650
Size: 351 1008 367 385 17 1452 86 12
Cuts at: 5650 5996 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1008 385 367 351 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2468 513
Size: 552 1403 4046
Fragments arranged by size:
4046 1403 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 2993 3517 4311 4331 2993
Size: 524 794 20 4663
Fragments arranged by size:
4663 794 524 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2376 5008 809
Size: 507 1060 2632 1802
Fragments arranged by size:
2632 1802 1060 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3448 4337 5966
Size: 54 1425 50 275 18 1618 889 1629
Cuts at: 5966 8
Size: 43
Fragments arranged by size:
1629 1618 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 1537
Size: 275 1636 4090
Fragments arranged by size:
4090 1636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2360 2454 3931 1353
Size: 696 311 94 1477 3423
Fragments arranged by size:
3423 1477 696 311 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4588 4962 5108 387
Size: 1064 138 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3096 3096
Size: 6001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4064 4661 5448 5716 1239
Size: 393 2432 597 787 268 1524
Fragments arranged by size:
2432 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2532 2784 2868 3036 3204 3288 3310 3688 2532
Size: 252 84 168 168 84 22 378 4845
Fragments arranged by size:
4845 378 252 168 168 84 84 22
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 5148 1678
Size: 3470 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2485 2517 4642
Size: 32 135 32 743 32 138 32 2125
Cuts at: 4642 4674 4716 4748 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2125 743 138 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 2545 5115 5299 1305
Size: 873 367 2570 184 2007
Fragments arranged by size:
2570 2007 873 367 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4179
Fragments arranged by size:
4179 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2473 2541 2709 2793 2877 2961 3045
Size: 648 1307 68 168 84 84 84 84
Cuts at: 3045 3129 3145 3213 3297 3959 4318 4477 518
Size: 84 16 68 84 662 359 159 2042
Fragments arranged by size:
2042 1307 662 648 359 168 159 84
84 84 84 84 84 68 68 16
MspA1I CmG'CkG
Cuts at: 1606 2249 3417 4094 5242 5487 1606
Size: 643 1168 677 1148 245 2120
Fragments arranged by size:
2120 1168 1148 677 643 245
NcoI C'CATG_G
Cuts at: 513 1065 2468 513
Size: 552 1403 4046
Fragments arranged by size:
4046 1403 552
NdeI CA'TA_TG
Cuts at: 387 3142 387
Size: 2755 3246
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NsiI A_TGCA'T
Cuts at: 2073 2958 2073
Size: 885 5116
NspI r_CATG'y
Cuts at: 1132 1229 2407 3359 4904 1132
Size: 97 1178 952 1545 2229
Fragments arranged by size:
2229 1545 1178 952 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 2403 4900 2403
Size: 2497 3504
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6001
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PstI C_TGCA'G
Cuts at: 830 3353 830
Size: 2523 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2249 2249
Size: 6001
SacI G_AGCT'C
Cuts at: 3098 3098
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SexAI A'CCwGG_T
Cuts at: 2214 2214
Size: 6001
SfcI C'TryA_G
Cuts at: 826 1045 3349 4484 5165 5356 826
Size: 219 2304 1135 681 191 1471
Fragments arranged by size:
2304 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2139 2929 4105 5006 5268 820
Size: 197 926 196 790 1176 901 262 1553
Fragments arranged by size:
1553 1176 926 901 790 262 197 196
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 2352 3925 5
Size: 47 2300 1573 2081
Fragments arranged by size:
2300 2081 1573 47
StuI AGG'CCT
Cuts at: 1517 2313 2999 3083 3167 1517
Size: 796 686 84 84 4351
Fragments arranged by size:
4351 796 686 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2097 2468 3440 513
Size: 552 1032 371 972 3074
Fragments arranged by size:
3074 1032 972 552 371
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2064 4028 4045 4198 4383 4828 325
Size: 1255 484 1964 17 153 185 445 1498
Fragments arranged by size:
1964 1498 1255 484 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4247 5959 96
Size: 274 80 33 51 1443 2270 1712 138
Fragments arranged by size:
2270 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 2502 2534 5961 5993 2321
Size: 32 149 32 3427 32 2329
Fragments arranged by size:
3427 2329 149 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 3105 4130 3105
Size: 1025 4976
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI