Restriction map for
pFN21AE0753
(Circular) (Six-base) MAPSORT of: pFN21AE0753.seq Check: 483 from: 1 to: 5719
pFN21AE0753 5719 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2298 3062 1937
Size: 361 764 4594
Fragments arranged by size:
4594 764 361
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 5166 5434 1632
Size: 2150 1384 268 1917
Fragments arranged by size:
2150 1917 1384 268
AfeI AGC'GCT
Cuts at: 2351 2351
Size: 5719
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 4618 5595 1203
Size: 3415 977 1327
Fragments arranged by size:
3415 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 2729 1166
Size: 1563 4156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2591 2623 2591
Size: 32 5687
AlwNI CAG_nnn'CTG
Cuts at: 3462 5034 3462
Size: 1572 4147
ApaLI G'TGCA_C
Cuts at: 2438 3776 4932 2438
Size: 1338 1156 3225
Fragments arranged by size:
3225 1338 1156
ApoI r'AATT_y
Cuts at: 1493 3028 3254 3406 5515 1493
Size: 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 3044 1870
Size: 73 349 752 4545
Fragments arranged by size:
4545 752 349 73
BamHI G'GATC_C
Cuts at: 2343 3049 3416 2343
Size: 706 367 4646
Fragments arranged by size:
4646 706 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 3040 4495 618
Size: 325 217 627 764 489 1455 1842
Fragments arranged by size:
1842 1455 764 627 489 325 217
BanII G_rGCy'C
Cuts at: 1391 2373 3038 1391
Size: 982 665 4072
Fragments arranged by size:
4072 982 665
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4748
BbvCI CC'TCA_GC
Cuts at: 2615 2615
Size: 5719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2416 2450 3910 3944 1903
Size: 34 479 34 1460 34 3678
Fragments arranged by size:
3678 1460 479 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2658 5714 2658
Size: 3056 2663
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2442 3780 4936 1790
Size: 652 1338 1156 2573
Fragments arranged by size:
2573 1338 1156 652
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3450 4408 442
Size: 416 53 83 399 24 2033 958 1753
Fragments arranged by size:
2033 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2307 2307
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2722 2834 4379 1239
Size: 1483 112 1545 2579
Fragments arranged by size:
2579 1545 1483 112
Bpu10I CC'TnA_GC
Cuts at: 2319 2615 2928 2319
Size: 296 313 5110
Fragments arranged by size:
5110 313 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2900 2978 3844 4709 5007 2900
Size: 78 866 865 298 3612
Fragments arranged by size:
3612 866 865 298 78
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 2196 5713 2196
Size: 3517 2202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2712 3526 3908 4543
Size: 53 83 186 936 1179 814 382 635
Cuts at: 4543 275
Size: 1451
Fragments arranged by size:
1451 1179 936 814 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2480 2510 1178
Size: 30 165 30 1077 30 4387
Fragments arranged by size:
4387 1077 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5719
BseYI C'CCAG_C
Cuts at: 2394 2453 2806 4922 5418 2394
Size: 59 353 2116 496 2695
Fragments arranged by size:
2695 2116 496 353 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 1163
Size: 573 669 4477
Fragments arranged by size:
4477 669 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3018 3169 3930 4958 1540
Size: 275 186 1017 151 761 1028 2301
Fragments arranged by size:
2301 1028 1017 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2288 2442 2916 3780 3865 4936 2288
Size: 154 474 864 85 1071 3071
Fragments arranged by size:
3071 1071 864 474 154 85
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2288 2373 2442 2916 3038 3780 3865
Size: 399 498 85 69 474 122 742 85
Cuts at: 3865 4936 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 498 474 399 122 85
85 69
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2427 3074 844
Size: 1583 647 3489
Fragments arranged by size:
3489 1583 647
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2409 4214 4388 65
Size: 1183 1161 1805 174 1396
Fragments arranged by size:
1805 1396 1183 1161 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5719
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstXI CCAn_nnnn'nTGG
Cuts at: 2887 2887
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 2343 2658 3049 3416 3801 3818 5270 5356
Size: 371 315 391 367 385 17 1452 86
Cuts at: 5356 5368 5714 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 391 385 371 367 346 315
86 17 12
BstZ17I GTA'TAC
Cuts at: 2299 2299
Size: 5719
BtgI C'CryG_G
Cuts at: 513 1065 2004 2101 513
Size: 552 939 97 4131
Fragments arranged by size:
4131 939 552 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 4029 4049 3235
Size: 794 20 4905
Fragments arranged by size:
4905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3166 4055
Size: 54 1425 50 275 18 268 1068 889
Cuts at: 4055 5684 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1068 889 275 268 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3649 1353
Size: 2296 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2722 2834 3782 4379 5166 5434 1239
Size: 393 1090 112 948 597 787 268 1524
Fragments arranged by size:
1524 1090 948 787 597 393 268 112
EcoO109I rG'GnC_Cy
Cuts at: 2358 2358
Size: 5719
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
EcoRV GAT'ATC
Cuts at: 2526 2526
Size: 5719
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 2353 4866 1678
Size: 675 2513 2531
Fragments arranged by size:
2531 2513 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2286 2305 2318 4360
Size: 32 135 32 701 13 19 13 2042
Cuts at: 4360 4392 4434 4466 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2042 701 135 42 32 32 32
32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3063 3309 669
Size: 988 281 1125 246 3079
Fragments arranged by size:
3079 1125 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2466 4833 5017 1305
Size: 1161 2367 184 2007
Fragments arranged by size:
2367 2007 1161 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 10
Size: 54 1425 343 268 3629
Fragments arranged by size:
3629 1425 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2729 3677 4036 4195 518
Size: 648 1563 948 359 159 2042
Fragments arranged by size:
2042 1563 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2398 3135 3812 4960 5205 1606
Size: 792 737 677 1148 245 2120
Fragments arranged by size:
2120 1148 792 737 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2101 513
Size: 552 939 97 4131
Fragments arranged by size:
4131 939 552 97
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 3077 4622 1132
Size: 97 1848 1545 2229
Fragments arranged by size:
2229 1848 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2404 1348
Size: 1056 4663
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2569 1108
Size: 1461 4258
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PstI C_TGCA'G
Cuts at: 830 3071 830
Size: 2241 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2398 2398
Size: 5719
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 3067 4202 4883 5074 826
Size: 219 2022 1135 681 191 1471
Fragments arranged by size:
2022 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2292 2915 2957 3823 4724 4986
Size: 197 926 349 623 42 866 901 262
Cuts at: 4986 820
Size: 1553
Fragments arranged by size:
1553 926 901 866 623 349 262 197
42
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 2186 1517
Size: 669 5050
StyI C'CwwG_G
Cuts at: 513 1065 2004 2101 3158 513
Size: 552 939 97 1057 3074
Fragments arranged by size:
3074 1057 939 552 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2899 2954 3746 3763 3916 4101 4546
Size: 1255 1319 55 792 17 153 185 445
Cuts at: 4546 325
Size: 1498
Fragments arranged by size:
1498 1319 1255 792 445 185 153 55
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3965 5677 96
Size: 274 80 33 51 1443 1988 1712 138
Fragments arranged by size:
1988 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2627 2108
Size: 519 5200
XhoI C'TCGA_G
Cuts at: 1943 2292 1943
Size: 349 5370
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI