(Circular) (Six-base) MAPSORT of: pFN21AE0750.seq Check: 5143 from: 1 to: 5983 pFN21AE0750 5983 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3326 1937 Size: 1389 4594 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 2291 2389 4046 5430 5698 1632 Size: 618 41 98 1657 1384 268 1917 Fragments arranged by size: 1917 1657 1384 618 268 98 41 AflII C'TTAA_G Cuts at: 820 1017 2423 820 Size: 197 1406 4380 Fragments arranged by size: 4380 1406 197 AflIII A'CryG_T Cuts at: 1203 3004 4882 5859 1203 Size: 1801 1878 977 1327 Fragments arranged by size: 1878 1801 1327 977 AgeI A'CCGG_T Cuts at: 2100 4814 2100 Size: 2714 3269 AhdI GACnn_n'nnGTC Cuts at: 2101 4712 2101 Size: 2611 3372 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5983 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2513 1184 Size: 1329 4654 AlwNI CAG_nnn'CTG Cuts at: 3726 5298 3726 Size: 1572 4411 ApaLI G'TGCA_C Cuts at: 2498 4040 5196 2498 Size: 1542 1156 3285 Fragments arranged by size: 3285 1542 1156 ApoI r'AATT_y Cuts at: 1493 2107 2534 2592 2702 2899 2928 3180 3292 Size: 614 427 58 110 197 29 252 112 Cuts at: 3292 3518 3670 5779 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 614 427 252 226 197 152 112 110 58 29 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 3308 1870 Size: 73 1365 4545 Fragments arranged by size: 4545 1365 73 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3304 4759 618 Size: 325 217 627 1517 1455 1842 Fragments arranged by size: 1842 1517 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2443 3302 1391 Size: 1052 859 4072 Fragments arranged by size: 4072 1052 859 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2395 2548 2611 3230 928 Size: 971 496 153 63 619 3681 Fragments arranged by size: 3681 971 619 496 153 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4174 4208 1903 Size: 34 2237 34 3678 Fragments arranged by size: 3678 2237 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2337 3875 5085 2337 Size: 1538 1210 3235 Fragments arranged by size: 3235 1538 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4594 136 Size: 107 122 71 631 605 2922 1525 Fragments arranged by size: 2922 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3152 5978 3152 Size: 2826 3157 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2502 4044 5200 1790 Size: 712 1542 1156 2573 Fragments arranged by size: 2573 1542 1156 712 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2349 3714 4672 Size: 416 53 83 399 24 932 1365 958 Cuts at: 4672 442 Size: 1753 Fragments arranged by size: 1753 1365 958 932 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2711 2711 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2553 3126 4643 1239 Size: 998 316 573 1517 2579 Fragments arranged by size: 2579 1517 998 573 316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4108 4973 5271 4108 Size: 865 298 4820 Fragments arranged by size: 4820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4646 882 Size: 617 3147 2219 Fragments arranged by size: 3147 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3774 493 Size: 711 2570 2702 Fragments arranged by size: 2702 2570 711 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3790 4172 4807 275 Size: 53 83 186 936 2257 382 635 1451 Fragments arranged by size: 2257 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2100 4416 4814 5088 5235 5646 2100 Size: 2316 398 274 147 411 2437 Fragments arranged by size: 2437 2316 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2551 2581 3267 3297 1178 Size: 30 165 30 1148 30 686 30 3864 Fragments arranged by size: 3864 1148 686 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 1186 Size: 991 4992 BseYI C'CCAG_C Cuts at: 5186 5682 5186 Size: 496 5487 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2817 1163 Size: 573 1081 4329 Fragments arranged by size: 4329 1081 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 4194 5222 1540 Size: 275 186 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2443 2502 4044 4129 5200 2443 Size: 59 1542 85 1071 3226 Fragments arranged by size: 3226 1542 1071 85 59 BsmI GAATG_Cn' Cuts at: 3494 3587 3494 Size: 93 5890 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2443 2502 3302 4044 4129 5200 1391 Size: 399 653 59 800 742 85 1071 2174 Fragments arranged by size: 2174 1071 800 742 653 399 85 59 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 3872 5602 3872 Size: 1730 4253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3338 844 Size: 2494 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3870 1394 Size: 429 2047 3507 Fragments arranged by size: 3507 2047 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4478 4652 65 Size: 1183 3230 174 1396 Fragments arranged by size: 3230 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 4627 4814 1700 Size: 110 290 2527 187 2869 Fragments arranged by size: 2869 2527 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 4043 5055 4043 Size: 1012 4971 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5983 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2166 2166 Size: 5983 BstYI r'GATC_y Cuts at: 1972 2888 3152 3313 3680 4065 4082 5534 5620 Size: 916 264 161 367 385 17 1452 86 Cuts at: 5620 5632 5978 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 916 385 367 346 264 161 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2026 513 Size: 552 961 4470 Fragments arranged by size: 4470 961 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5244 Fragments arranged by size: 5244 683 56 BtsI GCAGTG_nn' Cuts at: 2756 3499 4293 4313 2756 Size: 743 794 20 4426 Fragments arranged by size: 4426 794 743 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3052 3430 4319 Size: 54 1425 50 275 18 1222 378 889 Cuts at: 4319 5948 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1222 889 378 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3913 1353 Size: 2560 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4570 4944 5090 387 Size: 1064 138 2981 374 146 1280 Fragments arranged by size: 2981 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2441 2441 Size: 5983 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2250 2291 2389 2553 3126 4046 Size: 393 605 13 41 98 164 573 920 Cuts at: 4046 4643 5430 5698 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 920 787 605 597 573 393 268 164 98 41 13 EcoRI G'AATT_C Cuts at: 3180 3292 3670 3180 Size: 112 378 5493 Fragments arranged by size: 5493 378 112 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 5130 1678 Size: 3452 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2364 2396 4624 Size: 32 135 32 515 32 245 32 2228 Cuts at: 4624 4656 4698 4730 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2228 515 245 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5097 5281 1305 Size: 3792 184 2007 Fragments arranged by size: 3792 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3054 10 Size: 54 1425 343 1222 2939 Fragments arranged by size: 2939 1425 1222 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2953 3941 4300 4459 518 Size: 648 1329 458 988 359 159 2042 Fragments arranged by size: 2042 1329 988 648 458 359 159 MspA1I CmG'CkG Cuts at: 1606 3399 4076 5224 5469 1606 Size: 1793 677 1148 245 2120 Fragments arranged by size: 2120 1793 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2026 513 Size: 552 961 4470 Fragments arranged by size: 4470 961 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 2840 3008 3341 4886 1132 Size: 97 1611 168 333 1545 2229 Fragments arranged by size: 2229 1611 1545 333 168 97 PasI CC'CwG_GG Cuts at: 1348 2040 1348 Size: 692 5291 PciI A'CATG_T Cuts at: 3004 4882 3004 Size: 1878 4105 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5983 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2222 2254 4052 4084 5565 5597 2222 Size: 32 1798 32 1481 32 2608 Fragments arranged by size: 2608 1798 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PstI C_TGCA'G Cuts at: 830 3335 830 Size: 2505 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 SacI G_AGCT'C Cuts at: 2443 2443 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SfcI C'TryA_G Cuts at: 826 1045 2200 2365 3331 4466 5147 5338 826 Size: 219 1155 165 966 1135 681 191 1471 Fragments arranged by size: 1471 1155 1135 966 681 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2423 4087 4988 5250 820 Size: 197 926 480 1664 901 262 1553 Fragments arranged by size: 1664 1553 926 901 480 262 197 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3341 1132 Size: 2209 3774 SspI AAT'ATT Cuts at: 5 52 2334 3907 5 Size: 47 2282 1573 2081 Fragments arranged by size: 2282 2081 1573 47 StuI AGG'CCT Cuts at: 1517 2574 1517 Size: 1057 4926 StyI C'CwwG_G Cuts at: 513 1065 2026 3422 513 Size: 552 961 1396 3074 Fragments arranged by size: 3074 1396 961 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4010 4027 4180 4365 4810 325 Size: 1255 2430 17 153 185 445 1498 Fragments arranged by size: 2430 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3069 4229 5941 Size: 274 80 33 51 1443 1092 1160 1712 Cuts at: 5941 96 Size: 138 Fragments arranged by size: 1712 1443 1160 1092 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2368 2400 2551 2583 5943 5975 2368 Size: 32 151 32 3360 32 2376 Fragments arranged by size: 3360 2376 151 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 2807 2975 3143 3227 4112 2807 Size: 168 168 84 885 4678 Fragments arranged by size: 4678 885 168 168 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI