Restriction map for
pFN21AE0749
(Circular) (Six-base) MAPSORT of: pFN21AE0749.seq Check: 8655 from: 1 to: 5683
pFN21AE0749 5683 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2452 2452
Size: 5683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2166 2604 3026 1937
Size: 229 438 422 4594
Fragments arranged by size:
4594 438 422 229
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5130 5398 1632
Size: 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 4582 5559 1203
Size: 3379 977 1327
Fragments arranged by size:
3379 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 2627 1166
Size: 1461 4222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2467 2809 3426 4998 2467
Size: 342 617 1572 3152
Fragments arranged by size:
3152 1572 617 342
ApaI G_GGCC'C
Cuts at: 2049 2049
Size: 5683
ApaLI G'TGCA_C
Cuts at: 2504 2929 3740 4896 2504
Size: 425 811 1156 3291
Fragments arranged by size:
3291 1156 811 425
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AscI GG'CGCG_CC
Cuts at: 2444 2444
Size: 5683
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2381 2625 3008 1870
Size: 73 438 244 383 4545
Fragments arranged by size:
4545 438 383 244 73
BamHI G'GATC_C
Cuts at: 2649 3013 3380 2649
Size: 364 367 4952
Fragments arranged by size:
4952 367 364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2423 2621 2820 3004 4459
Size: 325 217 627 636 198 199 184 1455
Cuts at: 4459 618
Size: 1842
Fragments arranged by size:
1842 1455 636 627 325 217 199 198
184
BanII G_rGCy'C
Cuts at: 1391 2049 2196 2559 3002 1391
Size: 658 147 363 443 4072
Fragments arranged by size:
4072 658 443 363 147
BbeI G_GCGC'C
Cuts at: 2427 2427
Size: 5683
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3874 3908 1903
Size: 34 50 34 1853 34 3678
Fragments arranged by size:
3678 1853 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2253 4294 136
Size: 107 122 71 631 605 581 2041 1525
Fragments arranged by size:
2041 1525 631 605 581 122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2268 2508 2933 3744 4900 1790
Size: 259 219 240 425 811 1156 2573
Fragments arranged by size:
2573 1156 811 425 259 240 219
BmgBI CAC'GTC
Cuts at: 1318 2950 2964 1318
Size: 1632 14 4037
Fragments arranged by size:
4037 1632 14
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2808 2808
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2393 2778 4343 1239
Size: 1052 102 385 1565 2579
Fragments arranged by size:
2579 1565 1052 385 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2576 3808 4673 4971 2543
Size: 33 1232 865 298 3255
Fragments arranged by size:
3255 1232 865 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2794 4346 882
Size: 617 1295 1552 2219
Fragments arranged by size:
2219 1552 1295 617
BsaAI yAC'GTr
Cuts at: 493 1204 2215 3474 493
Size: 711 1011 1259 2702
Fragments arranged by size:
2702 1259 1011 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2319 2424 2901 3490
Size: 53 83 186 936 786 105 477 589
Cuts at: 3490 3872 4507 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 786 635 589 477 382 186
105 83 53
BsaWI w'CCGG_w
Cuts at: 2549 2778 2814 4116 4514 4788 4935 5346 2549
Size: 229 36 1302 398 274 147 411 2886
Fragments arranged by size:
2886 1302 411 398 274 229 147 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2531 2561 1178
Size: 30 165 30 1128 30 4300
Fragments arranged by size:
4300 1128 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2765 2797 1186
Size: 1579 32 4072
Fragments arranged by size:
4072 1579 32
BseYI C'CCAG_C
Cuts at: 2140 2695 2884 4886 5382 2140
Size: 555 189 2002 496 2441
Fragments arranged by size:
2441 2002 555 496 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2262 2849 3133 3894 4922 1540
Size: 275 186 261 587 284 761 1028 2301
Fragments arranged by size:
2301 1028 761 587 284 275 261 186
BsiHKAI G_wGCw'C
Cuts at: 2508 2559 2933 3744 3829 4900 2508
Size: 51 374 811 85 1071 3291
Fragments arranged by size:
3291 1071 811 374 85 51
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2196 2268 2508 2559 2933 3002
Size: 399 259 147 72 240 51 374 69
Cuts at: 3002 3744 3829 4900 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 374 259 240 147
85 72 69 51
BspEI T'CCGG_A
Cuts at: 2549 2778 5346 2549
Size: 229 2568 2886
Fragments arranged by size:
2886 2568 229
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2452 2550 2928 3038 844
Size: 1608 98 378 110 3489
Fragments arranged by size:
3489 1608 378 110 98
BsrBI CCG'CTC
Cuts at: 1394 1823 2227 2347 2377 2845 3570 1394
Size: 429 404 120 30 468 725 3507
Fragments arranged by size:
3507 725 468 429 404 120 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2386 4327 4514 1700
Size: 110 576 1941 187 2869
Fragments arranged by size:
2869 1941 576 187 110
BsrGI T'GTAC_A
Cuts at: 96 2631 96
Size: 2535 3148
BssHII G'CGCG_C
Cuts at: 1925 2310 2312 2444 1925
Size: 385 2 132 5164
Fragments arranged by size:
5164 385 132 2
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 2230 1184
Size: 1046 4637
BstYI r'GATC_y
Cuts at: 1972 2076 2649 2754 3013 3380 3765 3782 5234
Size: 104 573 105 259 367 385 17 1452
Cuts at: 5234 5320 5332 5678 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 573 385 367 346 259 105
104 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2668 513
Size: 552 1603 3528
Fragments arranged by size:
3528 1603 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2167 487
Size: 683 56 941 4003
Fragments arranged by size:
4003 941 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 2482 3199 3993 4013 2142
Size: 340 717 794 20 3812
Fragments arranged by size:
3812 794 717 340 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DraIII CAC_nnn'GTG
Cuts at: 2218 2688 2218
Size: 470 5213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2900 4690 809
Size: 507 1584 1790 1802
Fragments arranged by size:
1802 1790 1584 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2469 2679 2846
Size: 54 1425 50 275 18 639 210 167
Cuts at: 2846 2952 3130 4019 5648 8
Size: 106 178 889 1629 43
Fragments arranged by size:
1629 1425 889 639 275 210 178 167
106 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2846 3130 1537
Size: 275 1034 284 4090
Fragments arranged by size:
4090 1034 284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 2780 3613 1353
Size: 1052 375 833 3423
Fragments arranged by size:
3423 1052 833 375
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 2315 4270 4644 4790 387
Size: 1064 138 522 204 1955 374 146 1280
Fragments arranged by size:
1955 1280 1064 522 374 204 146 138
EcoICRI GAG'CTC
Cuts at: 2557 2557
Size: 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2393 2778 3746 4343 5130 5398
Size: 393 659 102 385 968 597 787 268
Cuts at: 5398 1239
Size: 1524
Fragments arranged by size:
1524 968 787 659 597 393 385 268
102
EcoO109I rG'GnC_Cy
Cuts at: 2395 2395
Size: 5683
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 5683
FspI TGC'GCA
Cuts at: 2288 4189 2288
Size: 1901 3782
HaeII r_GCGC'y
Cuts at: 1678 2427 2677 4830 1678
Size: 749 250 2153 2531
Fragments arranged by size:
2531 2153 749 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2531 2563 2834 2866 4324
Size: 32 135 32 959 32 271 32 1458
Cuts at: 4324 4356 4398 4430 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1458 959 271 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KasI G'GCGC_C
Cuts at: 2423 2423
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2954 10
Size: 54 1425 343 1122 2739
Fragments arranged by size:
2739 1425 1122 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2627 3641 4000 4159 518
Size: 648 1461 1014 359 159 2042
Fragments arranged by size:
2042 1461 1014 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2440 2493 2809 3099 3776 4924 5169 1606
Size: 834 53 316 290 677 1148 245 2120
Fragments arranged by size:
2120 1148 834 677 316 290 245 53
NaeI GCC'GGC
Cuts at: 2388 2388
Size: 5683
NarI GG'CG_CC
Cuts at: 2424 2424
Size: 5683
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5131
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NgoMIV G'CCGG_C
Cuts at: 2386 2386
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 3041 4586 1132
Size: 97 1812 1545 2229
Fragments arranged by size:
2229 1812 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2688 1108
Size: 1580 4103
PfoI T'CCnGG_A
Cuts at: 2904 2904
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PmlI CAC'GTG
Cuts at: 2215 2215
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 2395
Size: 5683
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PspOMI G'GGCC_C
Cuts at: 2045 2045
Size: 5683
PstI C_TGCA'G
Cuts at: 830 2085 3035 830
Size: 1255 950 3478
Fragments arranged by size:
3478 1255 950
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2493 2809 2493
Size: 316 5367
SacI G_AGCT'C
Cuts at: 2559 2559
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SapI GCTCTTCn'nnn_
Cuts at: 2780 2780
Size: 5683
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SexAI A'CCwGG_T
Cuts at: 2124 2124
Size: 5683
SfcI C'TryA_G
Cuts at: 826 1045 2081 3031 4166 4847 5038 826
Size: 219 1036 950 1135 681 191 1471
Fragments arranged by size:
1471 1135 1036 950 681 219 191
SfoI GGC'GCC
Cuts at: 2425 2425
Size: 5683
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 2591 3787 4688 4950 820
Size: 197 926 615 33 1196 901 262 1553
Fragments arranged by size:
1553 1196 926 901 615 262 197 33
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 2091 3122 513
Size: 552 1026 1031 3074
Fragments arranged by size:
3074 1031 1026 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 3710 3727 3880 4065 4510 325
Size: 1255 669 1461 17 153 185 445 1498
Fragments arranged by size:
1498 1461 1255 669 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2631 3929 5641
Size: 274 80 33 51 1443 654 1298 1712
Cuts at: 5641 96
Size: 138
Fragments arranged by size:
1712 1443 1298 654 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 2600 1316
Size: 1284 4399
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SacII SanDI
SfiI SrfI SwaI XcmI