Restriction map for
pFN21AE0745
(Circular) (Six-base) MAPSORT of: pFN21AE0745.seq Check: 3867 from: 1 to: 5665
pFN21AE0745 5665 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2570 3008 1937
Size: 633 438 4594
Fragments arranged by size:
4594 633 438
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 2964 3792 4165 1563
Size: 1401 828 373 3063
Fragments arranged by size:
3063 1401 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 3728 5112 5380 1632
Size: 610 1486 1384 268 1917
Fragments arranged by size:
1917 1486 1384 610 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 2753 4564 5541 1203
Size: 1550 1811 977 1327
Fragments arranged by size:
1811 1550 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AlwNI CAG_nnn'CTG
Cuts at: 3408 4980 3408
Size: 1572 4093
ApaI G_GGCC'C
Cuts at: 2535 2535
Size: 5665
ApaLI G'TGCA_C
Cuts at: 3722 4878 3722
Size: 1156 4509
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2990 1870
Size: 73 1047 4545
Fragments arranged by size:
4545 1047 73
BamHI G'GATC_C
Cuts at: 2103 2995 3362 2103
Size: 892 367 4406
Fragments arranged by size:
4406 892 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4441 618
Size: 325 217 627 1199 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2094 2158 2535 2778 2984 1391
Size: 703 64 377 243 206 4072
Fragments arranged by size:
4072 703 377 243 206 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2864 2898 3856 3890 1903
Size: 34 50 34 843 34 958 34 3678
Fragments arranged by size:
3678 958 843 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2515 3557 4767 2515
Size: 1042 1210 3413
Fragments arranged by size:
3413 1210 1042
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 136
Size: 107 122 71 631 605 2604 1525
Fragments arranged by size:
2604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2535 3726 4882 1790
Size: 745 1191 1156 2573
Fragments arranged by size:
2573 1191 1156 745
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2028 3396 4354
Size: 416 53 83 399 24 611 1368 958
Cuts at: 4354 442
Size: 1753
Fragments arranged by size:
1753 1368 958 611 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 4325 1239
Size: 795 2291 2579
Fragments arranged by size:
2579 2291 795
Bpu10I CC'TnA_GC
Cuts at: 2817 2817
Size: 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 2435 3790 4655 4953 2081
Size: 354 1355 865 298 2793
Fragments arranged by size:
2793 1355 865 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2261 2753 4328 882
Size: 617 762 492 1575 2219
Fragments arranged by size:
2219 1575 762 617 492
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5440
Fragments arranged by size:
5440 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2072 2084 2441 2444 1186
Size: 883 3 12 357 3 4407
Fragments arranged by size:
4407 883 357 12 3 3
BseYI C'CCAG_C
Cuts at: 2527 2560 4868 5364 2527
Size: 33 2308 496 2828
Fragments arranged by size:
2828 2308 496 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2633 1163
Size: 573 897 4195
Fragments arranged by size:
4195 897 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 2158 2778 3726 3811 4882 2094
Size: 64 620 948 85 1071 2877
Fragments arranged by size:
2877 1071 948 620 85 64
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2158 2535 2778 2984 3726 3811
Size: 399 304 64 377 243 206 742 85
Cuts at: 3811 4882 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 377 304 243 206
85 64
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2775 3020 844
Size: 1931 245 3489
Fragments arranged by size:
3489 1931 245
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2892 4160 4334 65
Size: 1183 1644 1268 174 1396
Fragments arranged by size:
1644 1396 1268 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2608 4309 4496 1700
Size: 110 798 1701 187 2869
Fragments arranged by size:
2869 1701 798 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 2027 4469 2027
Size: 2442 3223
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2103 2995 3362 3747 3764 5216 5302 5314
Size: 131 892 367 385 17 1452 86 12
Cuts at: 5314 5660 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 892 385 367 346 131 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2677 513
Size: 552 1612 3501
Fragments arranged by size:
3501 1612 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2159 3181 3975 3995 2159
Size: 1022 794 20 3829
Fragments arranged by size:
3829 1022 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2078 4672 809
Size: 507 762 2594 1802
Fragments arranged by size:
2594 1802 762 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2355 2550
Size: 54 1425 50 275 18 282 243 195
Cuts at: 2550 2833 3112 4001 5630 8
Size: 283 279 889 1629 43
Fragments arranged by size:
1629 1425 889 283 282 279 275 243
195 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2783 3595 1353
Size: 1430 812 3423
Fragments arranged by size:
3423 1430 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2092 2156 2776 2092
Size: 64 620 4981
Fragments arranged by size:
4981 620 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2242 3728 4325 5112 5380 1239
Size: 393 402 208 1486 597 787 268 1524
Fragments arranged by size:
1524 1486 787 597 402 393 268 208
EcoNI CCTnn'n_nnAGG
Cuts at: 2816 2816
Size: 5665
EcoO109I rG'GnC_Cy
Cuts at: 2410 2434 2531 2410
Size: 24 97 5544
Fragments arranged by size:
5544 97 24
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 4812 1678
Size: 3134 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 4306 4338 4380
Size: 32 135 32 794 32 1908 32 42
Cuts at: 4380 4412 1373
Size: 32 2626
Fragments arranged by size:
2626 1908 794 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 2753 5541 2753
Size: 2788 2877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 2357 2835 10
Size: 54 1425 343 282 243 478 2840
Fragments arranged by size:
2840 1425 478 343 282 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3623 3982 4141 518
Size: 648 2457 359 159 2042
Fragments arranged by size:
2457 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2508 2560 2679 3081 3758 4906 5151 1606
Size: 902 52 119 402 677 1148 245 2120
Fragments arranged by size:
2120 1148 902 677 402 245 119 52
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 3023 4568 1132
Size: 97 1794 1545 2229
Fragments arranged by size:
2229 1794 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 2434 2410
Size: 24 5641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PspOMI G'GGCC_C
Cuts at: 2531 2531
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2772 3017 830
Size: 1942 245 3478
Fragments arranged by size:
3478 1942 245
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
PvuII CAG'CTG
Cuts at: 2560 2560
Size: 5665
SacI G_AGCT'C
Cuts at: 2094 2158 2778 2094
Size: 64 620 4981
Fragments arranged by size:
4981 620 64
SacII CC_GC'GG
Cuts at: 2680 2680
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SapI GCTCTTCn'nnn_
Cuts at: 2783 2783
Size: 5665
SbfI CC_TGCA'GG
Cuts at: 2772 3017 2772
Size: 245 5420
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SexAI A'CCwGG_T
Cuts at: 2046 2046
Size: 5665
SfcI C'TryA_G
Cuts at: 826 1045 2768 3013 4148 4829 5020 826
Size: 219 1723 245 1135 681 191 1471
Fragments arranged by size:
1723 1471 1135 681 245 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 2450 3769 4670 4932 820
Size: 197 926 153 354 1319 901 262 1553
Fragments arranged by size:
1553 1319 926 901 354 262 197 153
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5665
StyI C'CwwG_G
Cuts at: 513 1065 2172 2841 3104 513
Size: 552 1107 669 263 3074
Fragments arranged by size:
3074 1107 669 552 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2777 3692 3709 3862 4047 4492 325
Size: 1255 1197 915 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1197 915 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 3911 5623
Size: 274 80 33 51 1443 234 1700 1712
Cuts at: 5623 96
Size: 138
Fragments arranged by size:
1712 1700 1443 274 234 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2337 2369 5625 5657 2337
Size: 32 3256 32 2345
Fragments arranged by size:
3256 2345 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2378 1316
Size: 1062 4603
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 2315 3794 2315
Size: 1479 4186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SanDI SfiI SfoI SrfI SwaI XcmI