Restriction map for
pFN21AE0744
(Circular) (Six-base) MAPSORT of: pFN21AE0744.seq Check: 5344 from: 1 to: 5977
pFN21AE0744 5977 bp
With 182 enzymes: SgfI *
March 28, 2012 12:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2407 3232 2407
Size: 825 5152
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2151 3787 278
Size: 53 83 186 936 615 1636 2468
Fragments arranged by size:
2468 1636 936 615 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2217 3320 1937
Size: 280 1103 4594
Fragments arranged by size:
4594 1103 280
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2267 2297 4040 5424 5692 1632
Size: 492 143 30 1743 1384 268 1917
Fragments arranged by size:
1917 1743 1384 492 268 143 30
AfeI AGC'GCT
Cuts at: 2462 2885 2462
Size: 423 5554
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 2709 4876 5853 1203
Size: 1506 2167 977 1327
Fragments arranged by size:
2167 1506 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 2702 1166
Size: 1536 4441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 2027 2119 2476 3720 5292 2027
Size: 92 357 1244 1572 2712
Fragments arranged by size:
2712 1572 1244 357 92
ApaI G_GGCC'C
Cuts at: 3201 3201
Size: 5977
ApaLI G'TGCA_C
Cuts at: 2516 4034 5190 2516
Size: 1518 1156 3303
Fragments arranged by size:
3303 1518 1156
ApoI r'AATT_y
Cuts at: 1493 3286 3512 3664 5773 1493
Size: 1793 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2534 2903 3002 3038 3173 3302 1870
Size: 73 591 369 99 36 135 129 4545
Fragments arranged by size:
4545 591 369 135 129 99 73 36
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2189 2633 2650 2777
Size: 325 217 627 294 108 444 17 127
Cuts at: 2777 2847 3043 3121 3139 3145 3163 3298 4753
Size: 70 196 78 18 6 18 135 1455
Cuts at: 4753 618
Size: 1842
Fragments arranged by size:
1842 1455 627 444 325 294 217 196
135 127 108 78 70 18 18 17
6
BanII G_rGCy'C
Cuts at: 1391 2442 2865 2904 2955 3180 3201 3296 1391
Size: 1051 423 39 51 225 21 95 4072
Fragments arranged by size:
4072 1051 423 225 95 51 39 21
BbeI G_GCGC'C
Cuts at: 2654 2781 2851 3047 2654
Size: 127 70 196 5584
Fragments arranged by size:
5584 196 127 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 928
Size: 971 213 4793
Fragments arranged by size:
4793 971 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2607 2670 4588
Size: 107 122 71 631 605 935 63 1918
Cuts at: 4588 136
Size: 1525
Fragments arranged by size:
1918 1525 935 631 605 122 107 71
63
BglII A'GATC_T
Cuts at: 3267 5972 3267
Size: 2705 3272
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2228 2520 3126 3156 3174 3201 4038
Size: 304 134 292 606 30 18 27 837
Cuts at: 4038 5194 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 837 606 304 292 134 30
27 18
BmgBI CAC'GTC
Cuts at: 1318 2350 2710 2958 1318
Size: 1032 360 248 4337
Fragments arranged by size:
4337 1032 360 248
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3708 4666 442
Size: 416 53 83 399 24 2291 958 1753
Fragments arranged by size:
2291 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 2918 4637 1239
Size: 1066 613 1719 2579
Fragments arranged by size:
2579 1719 1066 613
Bpu10I CC'TnA_GC
Cuts at: 2028 2028
Size: 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 3041 4102 4967 5265 2927
Size: 114 1061 865 298 3639
Fragments arranged by size:
3639 1061 865 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2651 2771 2778
Size: 53 83 186 936 615 503 120 7
Cuts at: 2778 2784 2848 3044 3784 4166 4801 275
Size: 6 64 196 740 382 635 1451
Fragments arranged by size:
1451 936 740 635 615 503 382 196
186 120 83 64 53 7 6
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2756 2786 1178
Size: 30 165 30 897 30 426 30 4369
Fragments arranged by size:
4369 897 426 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2308 2810 1186
Size: 1122 502 4353
Fragments arranged by size:
4353 1122 502
BseYI C'CCAG_C
Cuts at: 2179 2402 2476 2917 5180 5676 2179
Size: 223 74 441 2263 496 2480
Fragments arranged by size:
2480 2263 496 441 223 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2489 1163
Size: 573 753 4651
Fragments arranged by size:
4651 753 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2557 3208 3220 3427 4188 5216
Size: 275 186 556 651 12 207 761 1028
Cuts at: 5216 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 651 556 275 207 186
12
BsiHKAI G_wGCw'C
Cuts at: 2520 2730 2745 2955 4038 4123 5194 2520
Size: 210 15 210 1083 85 1071 3303
Fragments arranged by size:
3303 1083 1071 210 210 85 15
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2228 2442 2520 2730 2745 2865
Size: 399 304 134 214 78 210 15 120
Cuts at: 2865 2904 2955 3126 3156 3174 3180 3201 3296
Size: 39 51 171 30 18 6 21 95
Cuts at: 3296 4038 4123 5194 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 304 214 210 171
134 120 95 85 78 51 39 30
21 18 15 6
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 2407 3232 3332 844
Size: 1213 350 825 100 3489
Fragments arranged by size:
3489 1213 825 350 100
BsrBI CCG'CTC
Cuts at: 1394 1823 2040 2631 3055 3081 3864 1394
Size: 429 217 591 424 26 783 3507
Fragments arranged by size:
3507 783 591 429 424 217 26
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2084 2747 2973 3136 3142 3160 4621
Size: 110 274 663 226 163 6 18 1461
Cuts at: 4621 4808 1700
Size: 187 2869
Fragments arranged by size:
2869 1461 663 274 226 187 163 110
18 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 2988 2990 1925
Size: 1063 2 4912
Fragments arranged by size:
4912 1063 2
BssSI C'ACGA_G
Cuts at: 2948 4037 5049 2948
Size: 1089 1012 3876
Fragments arranged by size:
3876 1089 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2515 3247 794
Size: 1721 732 3524
Fragments arranged by size:
3524 1721 732
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 3267 3307 3674 4059 4076 5528 5614 5626
Size: 1295 40 367 385 17 1452 86 12
Cuts at: 5626 5972 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1295 385 367 346 86 40
17 12
Bsu36I CC'TnA_GG
Cuts at: 2445 2655 2445
Size: 210 5767
BtgI C'CryG_G
Cuts at: 513 1065 2759 2911 3255 513
Size: 552 1694 152 344 3235
Fragments arranged by size:
3235 1694 552 344 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 2849 3029 3493 4287 4307 2849
Size: 180 464 794 20 4519
Fragments arranged by size:
4519 794 464 180 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2213 2375 4984 809
Size: 507 897 162 2609 1802
Fragments arranged by size:
2609 1802 897 507 162
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2580 3205
Size: 54 1425 50 275 18 306 444 625
Cuts at: 3205 3217 3252 3258 3424 4313 5942 8
Size: 12 35 6 166 889 1629 43
Fragments arranged by size:
1629 1425 889 625 444 306 275 166
54 50 43 35 18 12 6
EagI C'GGCC_G
Cuts at: 1537 1812 3205 3217 3424 1537
Size: 275 1393 12 207 4090
Fragments arranged by size:
4090 1393 275 207 12
EarI CTCTTCn'nnn_
Cuts at: 1353 3907 1353
Size: 2554 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2953 2953
Size: 5977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2267 2297 2305 2918 4040 4637
Size: 393 492 143 30 8 613 1122 597
Cuts at: 4637 5424 5692 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1122 787 613 597 492 393 268
143 30 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2291 2291
Size: 5977
EcoO109I rG'GnC_Cy
Cuts at: 2116 2407 3197 3198 2116
Size: 291 790 1 4895
Fragments arranged by size:
4895 790 291 1
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FspI TGC'GCA
Cuts at: 2873 4483 2873
Size: 1610 4367
HaeII r_GCGC'y
Cuts at: 1678 2464 2489 2654 2781 2851 2887 2911 3047
Size: 786 25 165 127 70 36 24 136
Cuts at: 3047 5124 1678
Size: 2077 2531
Fragments arranged by size:
2531 2077 786 165 136 127 70 36
25 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2756 2788 2942 2974 4618
Size: 32 135 32 1184 32 154 32 1644
Cuts at: 4618 4650 4692 4724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1644 1184 154 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 3321 3567 669
Size: 988 281 268 1115 246 3079
Fragments arranged by size:
3079 1115 988 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KasI G'GCGC_C
Cuts at: 2650 2777 2847 3043 2650
Size: 127 70 196 5584
Fragments arranged by size:
5584 196 127 70
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5091 5275 1305
Size: 3786 184 2007
Fragments arranged by size:
3786 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3254 10
Size: 54 1425 343 1422 2733
Fragments arranged by size:
2733 1425 1422 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2237 2702 3935 4294 4453 518
Size: 648 843 228 465 1233 359 159 2042
Fragments arranged by size:
2042 1233 843 648 465 359 228 159
MspA1I CmG'CkG
Cuts at: 1606 2476 2512 2815 2913 3087 3393 4070 5218
Size: 870 36 303 98 174 306 677 1148
Cuts at: 5218 5463 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 870 677 306 303 245 174
98 36
NaeI GCC'GGC
Cuts at: 2975 2975
Size: 5977
NarI GG'CG_CC
Cuts at: 2651 2778 2848 3044 2651
Size: 127 70 196 5584
Fragments arranged by size:
5584 196 127 70
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5425
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NgoMIV G'CCGG_C
Cuts at: 2973 2973
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2113 2878 3335 4880 1132
Size: 97 884 765 457 1545 2229
Fragments arranged by size:
2229 1545 884 765 457 97
PasI CC'CwG_GG
Cuts at: 1348 2072 3193 1348
Size: 724 1121 4132
Fragments arranged by size:
4132 1121 724
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PfoI T'CCnGG_A
Cuts at: 2172 2172
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2407 2407
Size: 5977
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspOMI G'GGCC_C
Cuts at: 3197 3197
Size: 5977
PspXI vC'TCGA_Gb
Cuts at: 3038 3038
Size: 5977
PstI C_TGCA'G
Cuts at: 830 3329 830
Size: 2499 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2476 2512 2476
Size: 36 5941
RsrII CG'GwC_CG
Cuts at: 3210 3210
Size: 5977
SacI G_AGCT'C
Cuts at: 2955 2955
Size: 5977
SacII CC_GC'GG
Cuts at: 2914 2914
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SexAI A'CCwGG_T
Cuts at: 2208 2208
Size: 5977
SfcI C'TryA_G
Cuts at: 826 1045 3325 4460 5141 5332 826
Size: 219 2280 1135 681 191 1471
Fragments arranged by size:
2280 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2652 2779 2849 3045 2652
Size: 127 70 196 5584
Fragments arranged by size:
5584 196 127 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3004 3304 1872
Size: 1132 300 4545
Fragments arranged by size:
4545 1132 300
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2942 3038 3056 4081 4982 5244
Size: 197 926 999 96 18 1025 901 262
Cuts at: 5244 820
Size: 1553
Fragments arranged by size:
1553 1025 999 926 901 262 197 96
18
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 2167 152
Size: 2015 3962
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2922 3416 513
Size: 552 1857 494 3074
Fragments arranged by size:
3074 1857 552 494
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3089 3227 4004 4021 4174 4359 4804
Size: 1255 1509 138 777 17 153 185 445
Cuts at: 4804 325
Size: 1498
Fragments arranged by size:
1509 1498 1255 777 445 185 153 138
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4223 5935 96
Size: 274 80 33 51 1443 2246 1712 138
Fragments arranged by size:
2246 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 2351 1316
Size: 1035 4942
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 3038 1943
Size: 1095 4882
XmaI C'CCGG_G
Cuts at: 1870 3002 3302 1870
Size: 1132 300 4545
Fragments arranged by size:
4545 1132 300
XmnI GAAnn'nnTTC
Cuts at: 2282 4106 2282
Size: 1824 4153
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2149 3785 276
Size: 53 83 186 936 615 1636 2468
Fragments arranged by size:
2468 1636 936 615 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PsrI SanDI SapI SfiI SrfI
SwaI XcmI