Restriction map for
pFN21AE0734
(Circular) (Six-base) MAPSORT of: pFN21AE0734.seq Check: 4879 from: 1 to: 5971
pFN21AE0734 5971 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2285 2285
Size: 5971
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2524 3314 1937
Size: 587 790 4594
Fragments arranged by size:
4594 790 587
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2119 4034 5418 5686 1632
Size: 487 1915 1384 268 1917
Fragments arranged by size:
1917 1915 1384 487 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 3714 5286 3714
Size: 1572 4399
ApaLI G'TGCA_C
Cuts at: 2070 2468 4028 5184 2070
Size: 398 1560 1156 2857
Fragments arranged by size:
2857 1560 1156 398
ApoI r'AATT_y
Cuts at: 1493 3280 3506 3658 5767 1493
Size: 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3054 3296 1870
Size: 73 1111 242 4545
Fragments arranged by size:
4545 1111 242 73
AvrII C'CTAG_G
Cuts at: 2713 2713
Size: 5971
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 4747 618
Size: 325 217 627 1505 1455 1842
Fragments arranged by size:
1842 1505 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2178 2364 3163 3290 1391
Size: 787 186 799 127 4072
Fragments arranged by size:
4072 799 787 186 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2426 3257 928
Size: 971 527 831 3642
Fragments arranged by size:
3642 971 831 527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3083 3117 4162 4196 1903
Size: 34 1146 34 1045 34 3678
Fragments arranged by size:
3678 1146 1045 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3258 3388 3258
Size: 130 5841
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2472 4032 5188 1790
Size: 284 398 1560 1156 2573
Fragments arranged by size:
2573 1560 1156 398 284
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3702 4660 442
Size: 416 53 83 399 24 2285 958 1753
Fragments arranged by size:
2285 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2765
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2994 4631 1239
Size: 1139 616 1637 2579
Fragments arranged by size:
2579 1637 1139 616
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 4096 4961 5259 2750
Size: 1346 865 298 3462
Fragments arranged by size:
3462 1346 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2309 2989 4634 882
Size: 617 810 680 1645 2219
Fragments arranged by size:
2219 1645 810 680 617
BsaAI yAC'GTr
Cuts at: 493 1204 2243 2467 3028 3762 493
Size: 711 1039 224 561 734 2702
Fragments arranged by size:
2702 1039 734 711 561 224
BsaBI GATnn'nnATC
Cuts at: 2438 5965 2438
Size: 3527 2444
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2372 2402 1178
Size: 30 165 30 969 30 4747
Fragments arranged by size:
4747 969 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2086 2870 1186
Size: 900 784 4287
Fragments arranged by size:
4287 900 784
BseYI C'CCAG_C
Cuts at: 5174 5670 5174
Size: 496 5475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2313 1163
Size: 573 577 4821
Fragments arranged by size:
4821 577 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 2178 2248 2364 2472 4032 4117 5188 2074
Size: 104 70 116 108 1560 85 1071 2857
Fragments arranged by size:
2857 1560 1071 116 108 104 85 70
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2178 2248 2364 2472 3163 3290
Size: 399 284 104 70 116 108 691 127
Cuts at: 3290 4032 4117 5188 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 691 399 284 127 116
108 104 85 70
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2285 3326 844
Size: 1441 1041 3489
Fragments arranged by size:
3489 1441 1041
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4466 4640 65
Size: 1183 3218 174 1396
Fragments arranged by size:
3218 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4615 4802 1700
Size: 110 2805 187 2869
Fragments arranged by size:
2869 2805 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 3103 4775 3103
Size: 1672 4299
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2356
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966
Size: 1329 367 385 17 1452 86 12 346
Cuts at: 5966 1972
Size: 1977
Fragments arranged by size:
1977 1452 1329 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2349 3153 513
Size: 552 939 51 294 804 3331
Fragments arranged by size:
3331 939 804 552 294 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 2296 3487 4281 4301 2074
Size: 222 1191 794 20 3744
Fragments arranged by size:
3744 1191 794 222 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2574 3080 3418
Size: 54 1425 50 275 18 744 506 338
Cuts at: 3418 4307 5936 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 744 506 338 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2939 3105 3901 1353
Size: 1586 166 796 3423
Fragments arranged by size:
3423 1586 796 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2176 2362 2176
Size: 186 5785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2378 2994 4034 4631 5418 5686
Size: 393 487 259 616 1040 597 787 268
Cuts at: 5686 1239
Size: 1524
Fragments arranged by size:
1524 1040 787 616 597 487 393 268
259
EcoO109I rG'GnC_Cy
Cuts at: 2126 2126
Size: 5971
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
EcoRV GAT'ATC
Cuts at: 2792 2792
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 3265 5118 1678
Size: 1587 1853 2531
Fragments arranged by size:
2531 1853 1587
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3068 3100 4612 4644 4686
Size: 32 135 32 1496 32 1512 32 42
Cuts at: 4686 4718 1373
Size: 32 2626
Fragments arranged by size:
2626 1512 1496 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2525 3315 3561 669
Size: 988 281 587 790 246 3079
Fragments arranged by size:
3079 988 790 587 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2576 10
Size: 54 1425 343 744 3405
Fragments arranged by size:
3405 1425 744 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3168 3929 4288 4447 518
Size: 648 2002 761 359 159 2042
Fragments arranged by size:
2042 2002 761 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2446 3387 4064 5212 5457 1606
Size: 840 941 677 1148 245 2120
Fragments arranged by size:
2120 1148 941 840 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2055 2349 513
Size: 552 939 51 294 4135
Fragments arranged by size:
4135 939 552 294 51
NdeI CA'TA_TG
Cuts at: 387 2721 387
Size: 2334 3637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2549 3329 4874 1132
Size: 97 1320 780 1545 2229
Fragments arranged by size:
2229 1545 1320 780 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PmlI CAC'GTG
Cuts at: 2243 2243
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2126 2126
Size: 5971
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 2277 3541 2277
Size: 1264 4707
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2471 2503 3006 3038 2471
Size: 32 503 32 5404
Fragments arranged by size:
5404 503 32 32
PstI C_TGCA'G
Cuts at: 830 3323 830
Size: 2493 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2446 2446
Size: 5971
SacI G_AGCT'C
Cuts at: 2178 2364 2178
Size: 186 5785
SalI G'TCGA_C
Cuts at: 1936 2523 3313 1936
Size: 587 790 4594
Fragments arranged by size:
4594 790 587
SanDI GG'GwC_CC
Cuts at: 2126 2126
Size: 5971
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 3319 4454 5135 5326 826
Size: 219 2274 1135 681 191 1471
Fragments arranged by size:
2274 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 3054 4075 4976 5238 820
Size: 197 926 822 289 1021 901 262 1553
Fragments arranged by size:
1553 1021 926 901 822 289 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 2004 2055 2349 2365 2577 2713 3410
Size: 552 939 51 294 16 212 136 697
Cuts at: 3410 513
Size: 3074
Fragments arranged by size:
3074 939 697 552 294 212 136 51
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2418 3998 4015 4168 4353 4798 325
Size: 1255 838 1580 17 153 185 445 1498
Fragments arranged by size:
1580 1498 1255 838 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 4217 5929
Size: 274 80 33 51 1443 288 1952 1712
Cuts at: 5929 96
Size: 138
Fragments arranged by size:
1952 1712 1443 288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3203 3235 5931 5963 3203
Size: 32 2696 32 3211
Fragments arranged by size:
3211 2696 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 3054 1943
Size: 1111 4860
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspOMI PspXI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI