Restriction map for
pFN21AE0733
(Circular) (Six-base) MAPSORT of: pFN21AE0733.seq Check: 5570 from: 1 to: 5638
pFN21AE0733 5638 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2270 2270
Size: 5638
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3701 5085 5353 1632
Size: 2069 1384 268 1917
Fragments arranged by size:
2069 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 3381 4953 3381
Size: 1572 4066
ApaI G_GGCC'C
Cuts at: 2405 2405
Size: 5638
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2351 2963 1870
Size: 73 99 309 612 4545
Fragments arranged by size:
4545 612 309 99 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2627 2660 2627
Size: 33 5605
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 2289 2561 2799 2959
Size: 325 217 627 348 154 272 238 160
Cuts at: 2959 4414 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 348 325 272 238 217
160 154
BanII G_rGCy'C
Cuts at: 1391 2405 2751 2957 1391
Size: 1014 346 206 4072
Fragments arranged by size:
4072 1014 346 206
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 928
Size: 971 235 4432
Fragments arranged by size:
4432 971 235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2125 2159 3829 3863 1903
Size: 34 188 34 1670 34 3678
Fragments arranged by size:
3678 1670 188 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2918 4249 136
Size: 107 122 71 631 605 1246 1331 1525
Fragments arranged by size:
1525 1331 1246 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 2745 3055 2745
Size: 310 5328
Bme1580I G_kGCm'C
Cuts at: 1790 2292 2405 2566 2788 3699 4855 1790
Size: 502 113 161 222 911 1156 2573
Fragments arranged by size:
2573 1156 911 502 222 161 113
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2417 2684 3369
Size: 416 53 83 399 24 1000 267 685
Cuts at: 3369 4327 442
Size: 958 1753
Fragments arranged by size:
1753 1000 958 685 416 399 267 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 4298 1239
Size: 917 2142 2579
Fragments arranged by size:
2579 2142 917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5413
Fragments arranged by size:
5413 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2675 1186
Size: 1489 4149
BseYI C'CCAG_C
Cuts at: 2209 2758 4841 5337 2209
Size: 549 2083 496 2510
Fragments arranged by size:
2510 2083 549 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2298 1163
Size: 573 562 4503
Fragments arranged by size:
4503 573 562
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2751 3699 3784 4855 2751
Size: 948 85 1071 3534
Fragments arranged by size:
3534 1071 948 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2405 2566 2751 2788 2957 3699
Size: 399 502 113 161 185 37 169 742
Cuts at: 3699 3784 4855 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 399 185 169 161
113 85 37
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 2572 3527 5257 2572
Size: 955 1730 2953
Fragments arranged by size:
2953 1730 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2270 2993 844
Size: 1426 723 3489
Fragments arranged by size:
3489 1426 723
BsrBI CCG'CTC
Cuts at: 1394 1823 2356 3525 1394
Size: 429 533 1169 3507
Fragments arranged by size:
3507 1169 533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4133 4307 65
Size: 1183 2885 174 1396
Fragments arranged by size:
2885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2796 4282 4469 1700
Size: 110 986 1486 187 2869
Fragments arranged by size:
2869 1486 986 187 110
BsrGI T'GTAC_A
Cuts at: 96 2610 96
Size: 2514 3124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2474 2474
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2374 2628 2841 2968 3335 3720 3737 5189
Size: 402 254 213 127 367 385 17 1452
Cuts at: 5189 5275 5287 5633 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 402 385 367 346 254 213
127 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2473 2527 513
Size: 552 1408 54 3624
Fragments arranged by size:
3624 1408 552 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2258 2461 3154 3948 3968 2258
Size: 203 693 794 20 3928
Fragments arranged by size:
3928 794 693 203 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2411 2411
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2437 2658
Size: 54 1425 50 275 18 348 259 221
Cuts at: 2658 3085 3974 5603 8
Size: 427 889 1629 43
Fragments arranged by size:
1629 1425 889 427 348 275 259 221
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2832 3568 1353
Size: 1479 736 3423
Fragments arranged by size:
3423 1479 736
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2294 2783 4225 4599 4745 387
Size: 1064 138 705 489 1442 374 146 1280
Fragments arranged by size:
1442 1280 1064 705 489 374 146 138
EcoICRI GAG'CTC
Cuts at: 2749 2749
Size: 5638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 3701 4298 5085 5353 1239
Size: 393 524 1545 597 787 268 1524
Fragments arranged by size:
1545 1524 787 597 524 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2719 2120
Size: 599 5039
EcoO109I rG'GnC_Cy
Cuts at: 2027 2046 2401 2906 2027
Size: 19 355 505 4759
Fragments arranged by size:
4759 505 355 19
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2142 2174 4279
Size: 32 135 32 430 32 108 32 2105
Cuts at: 4279 4311 4353 4385 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2105 430 135 108 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2982 3228 669
Size: 988 281 72 972 246 3079
Fragments arranged by size:
3079 988 972 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2180 2439 10
Size: 54 1425 343 348 259 3209
Fragments arranged by size:
3209 1425 348 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2263 2472 3596 3955 4114 518
Size: 648 1020 77 209 1124 359 159 2042
Fragments arranged by size:
2042 1124 1020 648 359 209 159 77
MspA1I CmG'CkG
Cuts at: 1606 2427 3054 3731 4879 5124 1606
Size: 821 627 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 677 627 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5086
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2244 2996 4541 1132
Size: 97 1015 752 1545 2229
Fragments arranged by size:
2229 1545 1015 752 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2446 2667 1108
Size: 1338 221 4079
Fragments arranged by size:
4079 1338 221
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2046 2046
Size: 5638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PspOMI G'GGCC_C
Cuts at: 2401 2401
Size: 5638
PspXI vC'TCGA_Gb
Cuts at: 2351 2351
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2990 830
Size: 2160 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SacI G_AGCT'C
Cuts at: 2751 2751
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SanDI GG'GwC_CC
Cuts at: 2046 2046
Size: 5638
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2021 2986 4121 4802 4993 826
Size: 219 976 965 1135 681 191 1471
Fragments arranged by size:
1471 1135 976 965 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 2796 1700
Size: 1096 4542
SmaI CCC'GGG
Cuts at: 1872 2044 2965 1872
Size: 172 921 4545
Fragments arranged by size:
4545 921 172
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 3742 4643 4905 820
Size: 197 926 408 1391 901 262 1553
Fragments arranged by size:
1553 1391 926 901 408 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 3077 513
Size: 552 2012 3074
Fragments arranged by size:
3074 2012 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2264 2399 3665 3682 3835 4020 4465
Size: 1255 684 135 1266 17 153 185 445
Cuts at: 4465 325
Size: 1498
Fragments arranged by size:
1498 1266 1255 684 445 185 153 135
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2610 3884 5596
Size: 274 80 33 51 1443 633 1274 1712
Cuts at: 5596 96
Size: 138
Fragments arranged by size:
1712 1443 1274 633 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2260 2292 2453 2485 2664 2696 5598 5630 2260
Size: 32 161 32 179 32 2902 32 2268
Fragments arranged by size:
2902 2268 179 161 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2234 1316
Size: 918 4720
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 2351 1943
Size: 408 5230
XmaI C'CCGG_G
Cuts at: 1870 2042 2963 1870
Size: 172 921 4545
Fragments arranged by size:
4545 921 172
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI