Restriction map for
pFN21AE0732
(Circular) (Six-base) MAPSORT of: pFN21AE0732.seq Check: 870 from: 1 to: 5965
pFN21AE0732 5965 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2529 3308 1937
Size: 592 779 4594
Fragments arranged by size:
4594 779 592
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2760 4028 5412 5680 1632
Size: 1128 1268 1384 268 1917
Fragments arranged by size:
1917 1384 1268 1128 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 4864 5841 1203
Size: 3661 977 1327
Fragments arranged by size:
3661 1327 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2358 1184
Size: 1174 4791
AlwNI CAG_nnn'CTG
Cuts at: 2558 3708 5280 2558
Size: 1150 1572 3243
Fragments arranged by size:
3243 1572 1150
ApaI G_GGCC'C
Cuts at: 2176 2176
Size: 5965
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 2396 3274 3500 3652 5761 1493
Size: 903 878 226 152 2109 1697
Fragments arranged by size:
2109 1697 903 878 226 152
AscI GG'CGCG_CC
Cuts at: 2165 2165
Size: 5965
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 3225 3290 1870
Size: 73 1282 65 4545
Fragments arranged by size:
4545 1282 73 65
BamHI G'GATC_C
Cuts at: 2408 3295 3662 2408
Size: 887 367 4711
Fragments arranged by size:
4711 887 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2247 3286 4741 618
Size: 325 217 627 460 1039 1455 1842
Fragments arranged by size:
1842 1455 1039 627 460 325 217
BanII G_rGCy'C
Cuts at: 1391 2176 2729 3284 1391
Size: 785 553 555 4072
Fragments arranged by size:
4072 785 555 553
BbeI G_GCGC'C
Cuts at: 2251 2251
Size: 5965
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4994
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 1903
Size: 34 2219 34 3678
Fragments arranged by size:
3678 2219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2470 2725 2797 3857 5067 2470
Size: 255 72 1060 1210 3368
Fragments arranged by size:
3368 1210 1060 255 72
BclI T'GATC_A
Cuts at: 1548 3067 3106 1548
Size: 1519 39 4407
Fragments arranged by size:
4407 1519 39
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2110 4576 136
Size: 107 122 71 631 605 438 2466 1525
Fragments arranged by size:
2466 1525 631 605 438 122 107 71
BglII A'GATC_T
Cuts at: 2491 5960 2491
Size: 3469 2496
BlpI GC'TnA_GC
Cuts at: 3382 3382
Size: 5965
Bme1580I G_kGCm'C
Cuts at: 1790 2176 4026 5182 1790
Size: 386 1850 1156 2573
Fragments arranged by size:
2573 1850 1156 386
BmgBI CAC'GTC
Cuts at: 1318 2185 1318
Size: 867 5098
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2568 3153 3696
Size: 416 53 83 399 24 1151 585 543
Cuts at: 3696 4654 442
Size: 958 1753
Fragments arranged by size:
1753 1151 958 585 543 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2120 1056
Size: 1064 4901
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4625 1239
Size: 3386 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2488 4090 4955 5253 2488
Size: 1602 865 298 3200
Fragments arranged by size:
3200 1602 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4628 882
Size: 617 3129 2219
Fragments arranged by size:
3129 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2248 3772 4154 4789
Size: 53 83 186 936 715 1524 382 635
Cuts at: 4789 275
Size: 1451
Fragments arranged by size:
1524 1451 936 715 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4398 4796 5070 5217 5628 4398
Size: 398 274 147 411 4735
Fragments arranged by size:
4735 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5740
Fragments arranged by size:
5740 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 5034
BseYI C'CCAG_C
Cuts at: 2144 2175 2193 2472 5168 5664 2144
Size: 31 18 279 2696 496 2445
Fragments arranged by size:
2696 2445 496 279 31 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2518 1163
Size: 573 782 4610
Fragments arranged by size:
4610 782 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2099 2105 3415 4176 5204 1540
Size: 275 186 98 6 1310 761 1028 2301
Fragments arranged by size:
2301 1310 1028 761 275 186 98 6
BsiHKAI G_wGCw'C
Cuts at: 3209 4026 4111 5182 3209
Size: 817 85 1071 3992
Fragments arranged by size:
3992 1071 817 85
BsmI GAATG_Cn'
Cuts at: 3476 3569 3476
Size: 93 5872
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 2729 3209 3284 4026 4111 5182
Size: 399 386 553 480 75 742 85 1071
Cuts at: 5182 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 553 480 399 386 85
75
BspEI T'CCGG_A
Cuts at: 5628 5628
Size: 5965
BspHI T'CATG_A
Cuts at: 3854 5584 3854
Size: 1730 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3320 844
Size: 2476 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2057 2125 3852 1394
Size: 429 234 68 1727 3507
Fragments arranged by size:
3507 1727 429 234 68
BsrDI GCAATG_nn'
Cuts at: 65 1248 4460 4634 65
Size: 1183 3212 174 1396
Fragments arranged by size:
3212 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4609 4796 1700
Size: 110 2799 187 2869
Fragments arranged by size:
2869 2799 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 2165 2327 1925
Size: 240 162 5563
Fragments arranged by size:
5563 240 162
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5965
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstXI CCAn_nnnn'nTGG
Cuts at: 2897 2897
Size: 5965
BstYI r'GATC_y
Cuts at: 1972 2254 2408 2491 2548 2659 3295 3662 4047
Size: 282 154 83 57 111 636 367 385
Cuts at: 4047 4064 5516 5602 5614 5960 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 636 385 367 346 282 154
111 86 83 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2099 2108 2799 513
Size: 552 939 95 9 691 3679
Fragments arranged by size:
3679 939 691 552 95 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 3481 4275 4295 3481
Size: 794 20 5151
Fragments arranged by size:
5151 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 2275 2682 3269 2275
Size: 407 587 4971
Fragments arranged by size:
4971 587 407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2354 4972 809
Size: 507 1038 2618 1802
Fragments arranged by size:
2618 1802 1038 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2096 2102 3019
Size: 54 1425 50 275 18 266 6 917
Cuts at: 3019 3196 3412 4301 5930 8
Size: 177 216 889 1629 43
Fragments arranged by size:
1629 1425 917 889 275 266 216 177
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2096 2102 3412 1537
Size: 275 284 6 1310 4090
Fragments arranged by size:
4090 1310 284 275 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 2818 2850 2971 3895 1353
Size: 911 554 32 121 924 3423
Fragments arranged by size:
3423 924 911 554 121 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2140 2252 2287 3144 4552 4926
Size: 1064 138 551 112 35 857 1408 374
Cuts at: 4926 5072 387
Size: 146 1280
Fragments arranged by size:
1408 1280 1064 857 551 374 146 138
112 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2760 4028 4625 5412 5680 1239
Size: 393 1128 1268 597 787 268 1524
Fragments arranged by size:
1524 1268 1128 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2011 2011
Size: 5965
EcoRI G'AATT_C
Cuts at: 2396 3274 3652 2396
Size: 878 378 4709
Fragments arranged by size:
4709 878 378
FspI TGC'GCA
Cuts at: 4471 4471
Size: 5965
HaeII r_GCGC'y
Cuts at: 1678 2182 2251 5112 1678
Size: 504 69 2861 2531
Fragments arranged by size:
2861 2531 504 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2400 2416 2432 2448 2492
Size: 32 135 32 828 16 16 16 44
Cuts at: 2492 2524 4606 4638 4680 4712 1373
Size: 32 2082 32 42 32 2626
Fragments arranged by size:
2626 2082 828 135 44 42 32 32
32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3309 3555 669
Size: 988 281 1371 246 3079
Fragments arranged by size:
3079 1371 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2676 748
Size: 1928 4037
HpaI GTT'AAC
Cuts at: 3555 3555
Size: 5965
KasI G'GCGC_C
Cuts at: 2247 2247
Size: 5965
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5079 5263 1305
Size: 3774 184 2007
Fragments arranged by size:
3774 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3198 10
Size: 54 1425 343 1366 2777
Fragments arranged by size:
2777 1425 1366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3923 4282 4441 518
Size: 648 2757 359 159 2042
Fragments arranged by size:
2757 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2110 2306 2990 3381 4058 5206 5451
Size: 495 9 196 684 391 677 1148 245
Cuts at: 5451 1606
Size: 2120
Fragments arranged by size:
2120 1148 684 677 495 391 245 196
9
NarI GG'CG_CC
Cuts at: 2248 2248
Size: 5965
NcoI C'CATG_G
Cuts at: 513 1065 2004 2799 513
Size: 552 939 795 3679
Fragments arranged by size:
3679 939 795 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5965
NheI G'CTAG_C
Cuts at: 1052 2116 1052
Size: 1064 4901
NotI GC'GGCC_GC
Cuts at: 2096 2102 3412 2096
Size: 6 1310 4649
Fragments arranged by size:
4649 1310 6
NspI r_CATG'y
Cuts at: 1132 1229 3323 4868 1132
Size: 97 2094 1545 2229
Fragments arranged by size:
2229 2094 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5965
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2558 1108
Size: 1450 4515
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 2011
Size: 5965
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5965
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PspOMI G'GGCC_C
Cuts at: 2172 2172
Size: 5965
PstI C_TGCA'G
Cuts at: 830 2989 3317 830
Size: 2159 328 3478
Fragments arranged by size:
3478 2159 328
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
PvuII CAG'CTG
Cuts at: 2990 2990
Size: 5965
SacII CC_GC'GG
Cuts at: 2102 2111 2102
Size: 9 5956
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SapI GCTCTTCn'nnn_
Cuts at: 2850 2850
Size: 5965
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 2504 4213 5925 2504
Size: 1709 1712 2544
Fragments arranged by size:
2544 1712 1709
SfcI C'TryA_G
Cuts at: 826 1045 2985 3115 3190 3313 4448 5129 5320
Size: 219 1940 130 75 123 1135 681 191
Cuts at: 5320 826
Size: 1471
Fragments arranged by size:
1940 1471 1135 681 219 191 130 123
75
SfoI GGC'GCC
Cuts at: 2249 2249
Size: 5965
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 3292 1872
Size: 1420 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2467 3225 4069 4970 5232 820
Size: 197 926 524 758 844 901 262 1553
Fragments arranged by size:
1553 926 901 844 758 524 262 197
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 3323 1132
Size: 2191 3774
SspI AAT'ATT
Cuts at: 5 52 2759 2788 3889 5
Size: 47 2707 29 1101 2081
Fragments arranged by size:
2707 2081 1101 47 29
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 2004 2799 3046 3404 513
Size: 552 939 795 247 358 3074
Fragments arranged by size:
3074 939 795 552 358 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2705 3992 4009 4162 4347 4792 325
Size: 1255 1125 1287 17 153 185 445 1498
Fragments arranged by size:
1498 1287 1255 1125 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 2502 4211
Size: 274 80 33 51 1443 402 123 1709
Cuts at: 4211 5923 96
Size: 1712 138
Fragments arranged by size:
1712 1709 1443 402 274 138 123 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5925 5957 5925
Size: 32 5933
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 3301 3301
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 3225 1943
Size: 1282 4683
XmaI C'CCGG_G
Cuts at: 1870 3290 1870
Size: 1420 4545
XmnI GAAnn'nnTTC
Cuts at: 4094 4094
Size: 5965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SrfI SwaI XcmI