Restriction map for
pFN21AE0728
(Circular) (Six-base) MAPSORT of: pFN21AE0728.seq Check: 2520 from: 1 to: 5959
pFN21AE0728 5959 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2886 2886
Size: 5959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2718 3302 1937
Size: 781 584 4594
Fragments arranged by size:
4594 781 584
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2425 2896 4022 5406 5674 1632
Size: 793 471 1126 1384 268 1917
Fragments arranged by size:
1917 1384 1126 793 471 268
AfeI AGC'GCT
Cuts at: 2308 2308
Size: 5959
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 2644 4858 5835 1203
Size: 1441 2214 977 1327
Fragments arranged by size:
2214 1441 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3005 1184
Size: 1821 4138
AlwNI CAG_nnn'CTG
Cuts at: 2111 3702 5274 2111
Size: 1591 1572 2796
Fragments arranged by size:
2796 1591 1572
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2729 3268 3494 3646 5755 1493
Size: 1236 539 226 152 2109 1697
Fragments arranged by size:
2109 1697 1236 539 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2240 3284 1870
Size: 73 297 1044 4545
Fragments arranged by size:
4545 1044 297 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3229 3262 3229
Size: 33 5926
BamHI G'GATC_C
Cuts at: 2452 3289 3656 2452
Size: 837 367 4755
Fragments arranged by size:
4755 837 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 2597 2923 3131 3280
Size: 325 217 627 537 273 326 208 149
Cuts at: 3280 4735 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 537 326 325 273 217
208 149
BanII G_rGCy'C
Cuts at: 1391 2294 3278 1391
Size: 903 984 4072
Fragments arranged by size:
4072 984 903
BbeI G_GCGC'C
Cuts at: 2927 2927
Size: 5959
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2543 2577 2717 2751 4150
Size: 34 50 34 522 34 140 34 1399
Cuts at: 4150 4184 1903
Size: 34 3678
Fragments arranged by size:
3678 1399 522 140 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2183 2847 3230 3851 5061 2183
Size: 664 383 621 1210 3081
Fragments arranged by size:
3081 1210 664 621 383
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2416 4570
Size: 107 122 71 631 605 337 407 2154
Cuts at: 4570 136
Size: 1525
Fragments arranged by size:
2154 1525 631 605 407 337 122 107
71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 2130 3376 2130
Size: 1246 4713
Bme1580I G_kGCm'C
Cuts at: 1790 2794 4020 5176 1790
Size: 1004 1226 1156 2573
Fragments arranged by size:
2573 1226 1156 1004
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2143 2790 3690
Size: 416 53 83 399 24 726 647 900
Cuts at: 3690 4648 442
Size: 958 1753
Fragments arranged by size:
1753 958 900 726 647 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3093 4619 1239
Size: 1854 1526 2579
Fragments arranged by size:
2579 1854 1526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2001 4084 4949 5247 2001
Size: 2083 865 298 2713
Fragments arranged by size:
2713 2083 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3200 4622 882
Size: 617 1701 1422 2219
Fragments arranged by size:
2219 1701 1422 617
BsaAI yAC'GTr
Cuts at: 493 1204 2645 3750 493
Size: 711 1441 1105 2702
Fragments arranged by size:
2702 1441 1105 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2924 3766 4148
Size: 53 83 186 936 479 912 842 382
Cuts at: 4148 4783 275
Size: 635 1451
Fragments arranged by size:
1451 936 912 842 635 479 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2455 4392 4790 5064 5211 5622 2455
Size: 1937 398 274 147 411 2792
Fragments arranged by size:
2792 1937 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5959
BseYI C'CCAG_C
Cuts at: 3024 5162 5658 3024
Size: 2138 496 3325
Fragments arranged by size:
3325 2138 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2733 1163
Size: 573 997 4389
Fragments arranged by size:
4389 997 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2859 2893 3409 4170 5198 1540
Size: 275 186 858 34 516 761 1028 2301
Fragments arranged by size:
2301 1028 858 761 516 275 186 34
BsiHKAI G_wGCw'C
Cuts at: 2247 4020 4105 5176 2247
Size: 1773 85 1071 3030
Fragments arranged by size:
3030 1773 1071 85
BsmI GAATG_Cn'
Cuts at: 3145 3470 3563 3145
Size: 325 93 5541
Fragments arranged by size:
5541 325 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2019 2323 2019
Size: 304 5655
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2294 2794 3278 4020 4105 5176
Size: 399 457 47 500 484 742 85 1071
Cuts at: 5176 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 500 484 457 399 85
47
BspEI T'CCGG_A
Cuts at: 2455 5622 2455
Size: 3167 2792
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2886 3314 844
Size: 2042 428 3489
Fragments arranged by size:
3489 2042 428
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2826 3209 4454 4628 65
Size: 1183 1578 383 1245 174 1396
Fragments arranged by size:
1578 1396 1245 1183 383 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 4603 4790 1700
Size: 110 249 2544 187 2869
Fragments arranged by size:
2869 2544 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 2784 2901 96
Size: 2688 117 3154
Fragments arranged by size:
3154 2688 117
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 2302 4019 5031 2302
Size: 1717 1012 3230
Fragments arranged by size:
3230 1717 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2111 794
Size: 1317 4642
BstBI TT'CG_AA
Cuts at: 2553 4763 2553
Size: 2210 3749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2452 2774 3289 3656 4041 4058 5510 5596
Size: 480 322 515 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 515 480 385 367 346 322
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2178 2232 2601 2853 2887 2919 513
Size: 552 1113 54 369 252 34 32 3553
Fragments arranged by size:
3553 1113 552 369 252 54 34 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3039 3475 4269 4289 3039
Size: 436 794 20 4709
Fragments arranged by size:
4709 794 436 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2362 2856 3406
Size: 54 1425 50 275 18 532 494 550
Cuts at: 3406 4295 5924 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 550 532 494 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2856 3406 1537
Size: 275 1044 550 4090
Fragments arranged by size:
4090 1044 550 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3889 1353
Size: 2536 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2312 2967 4546 4920 5066 387
Size: 1064 138 723 655 1579 374 146 1280
Fragments arranged by size:
1579 1280 1064 723 655 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2425 2896 3093 4022 4619 5406 5674
Size: 393 793 471 197 929 597 787 268
Cuts at: 5674 1239
Size: 1524
Fragments arranged by size:
1524 929 793 787 597 471 393 268
197
EcoRI G'AATT_C
Cuts at: 2729 3268 3646 2729
Size: 539 378 5042
Fragments arranged by size:
5042 539 378
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2310 2927 5106 1678
Size: 632 617 2179 2531
Fragments arranged by size:
2531 2179 632 617
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373
Size: 32 135 32 3028 32 42 32 2626
Fragments arranged by size:
3028 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2671 3303 3549 669
Size: 988 281 733 632 246 3079
Fragments arranged by size:
3079 988 733 632 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2923 2923
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2364 10
Size: 54 1425 343 532 3605
Fragments arranged by size:
3605 1425 532 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2037 3183 3917 4276 4435 518
Size: 648 871 1146 734 359 159 2042
Fragments arranged by size:
2042 1146 871 734 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2111 2180 2855 2889 2921 3047 3375 4052
Size: 505 69 675 34 32 126 328 677
Cuts at: 4052 5200 5445 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 505 328 245 126
69 34 32
NarI GG'CG_CC
Cuts at: 2924 2924
Size: 5959
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 2856 3406 2856
Size: 550 5409
NspI r_CATG'y
Cuts at: 1132 1229 2426 3317 4862 1132
Size: 97 1197 891 1545 2229
Fragments arranged by size:
2229 1545 1197 891 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2077 2448 2077
Size: 371 5588
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PmlI CAC'GTG
Cuts at: 2645 2645
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2997 3311 830
Size: 2167 314 3478
Fragments arranged by size:
3478 2167 314
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacII CC_GC'GG
Cuts at: 2181 2856 2890 2922 2181
Size: 675 34 32 5218
Fragments arranged by size:
5218 675 34 32
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2719 2993 3052 3307 4442 5123 5314
Size: 219 1674 274 59 255 1135 681 191
Cuts at: 5314 826
Size: 1471
Fragments arranged by size:
1674 1471 1135 681 274 255 219 191
59
SfoI GGC'GCC
Cuts at: 2925 2925
Size: 5959
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2016 4063 4964 5226 820
Size: 197 926 73 2047 901 262 1553
Fragments arranged by size:
2047 1553 926 901 262 197 73
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 3398 513
Size: 552 2333 3074
Fragments arranged by size:
3074 2333 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2699 3986 4003 4156 4341 4786 325
Size: 1255 1119 1287 17 153 185 445 1498
Fragments arranged by size:
1498 1287 1255 1119 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2217 2784 2901
Size: 274 80 33 51 1443 240 567 117
Cuts at: 2901 4205 5917 96
Size: 1304 1712 138
Fragments arranged by size:
1712 1443 1304 567 274 240 138 117
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PstI PvuI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI
SapI SexAI SfiI SrfI SwaI XcmI