Restriction map for
pFN21AE0717
(Circular) (Six-base) MAPSORT of: pFN21AE0717.seq Check: 2303 from: 1 to: 5620
pFN21AE0717 5620 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 3683 5067 5335 1632
Size: 629 1422 1384 268 1917
Fragments arranged by size:
1917 1422 1384 629 268
AfeI AGC'GCT
Cuts at: 2141 2141
Size: 5620
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 2404 4349 2404
Size: 1945 3675
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2615 2647 2615
Size: 32 5588
AlwNI CAG_nnn'CTG
Cuts at: 2844 3363 4935 2844
Size: 519 1572 3529
Fragments arranged by size:
3529 1572 519
ApaI G_GGCC'C
Cuts at: 2805 2805
Size: 5620
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2011 2929 3155 3307 5416 1493
Size: 518 918 226 152 2109 1697
Fragments arranged by size:
2109 1697 918 518 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 2577 2945 1870
Size: 73 313 321 368 4545
Fragments arranged by size:
4545 368 321 313 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2279 2369 2941 4396 618
Size: 325 217 627 492 90 572 1455 1842
Fragments arranged by size:
1842 1455 627 572 492 325 217 90
BanII G_rGCy'C
Cuts at: 1391 2805 2939 1391
Size: 1414 134 4072
Fragments arranged by size:
4072 1414 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BbvCI CC'TCA_GC
Cuts at: 2334 2334
Size: 5620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2375 2409 2899 2933 3811 3845 1903
Size: 34 438 34 490 34 878 34 3678
Fragments arranged by size:
3678 878 490 438 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2076 2198 2592
Size: 107 122 71 631 605 404 122 394
Cuts at: 2592 4231 136
Size: 1639 1525
Fragments arranged by size:
1639 1525 631 605 404 394 122 122
107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2284 2805 3681 4837 1790
Size: 494 521 876 1156 2573
Fragments arranged by size:
2573 1156 876 521 494
BmgBI CAC'GTC
Cuts at: 1318 2329 1318
Size: 1011 4609
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4280 1239
Size: 3041 2579
Bpu10I CC'TnA_GC
Cuts at: 2334 2334
Size: 5620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 4610 4908 3745
Size: 865 298 4457
Fragments arranged by size:
4457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2610 4283 882
Size: 617 1111 1673 2219
Fragments arranged by size:
2219 1673 1111 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 3427 3809 4444
Size: 53 83 186 936 657 1237 382 635
Cuts at: 4444 275
Size: 1451
Fragments arranged by size:
1451 1237 936 657 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2546 4053 4451 4725 4872 5283 2546
Size: 1507 398 274 147 411 2883
Fragments arranged by size:
2883 1507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 1186
Size: 1009 4611
BseYI C'CCAG_C
Cuts at: 2844 4823 5319 2844
Size: 1979 496 3145
Fragments arranged by size:
3145 1979 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 1163
Size: 573 336 4711
Fragments arranged by size:
4711 573 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2587 2596 2854 3070 3831 4859
Size: 275 186 586 9 258 216 761 1028
Cuts at: 4859 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 586 275 258 216 186
9
BsiHKAI G_wGCw'C
Cuts at: 2355 3681 3766 4837 2355
Size: 1326 85 1071 3138
Fragments arranged by size:
3138 1326 1071 85
BsmI GAATG_Cn'
Cuts at: 3131 3224 3131
Size: 93 5527
BsmBI CGTCTCn'nnnn_
Cuts at: 2319 2319
Size: 5620
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2355 2805 2939 3681 3766 4837
Size: 399 494 71 450 134 742 85 1071
Cuts at: 4837 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 494 450 399 134 85
71
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2814 3507 1394
Size: 429 991 693 3507
Fragments arranged by size:
3507 991 693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2950 3317 3702 3719 5171 5257 5269 5615
Size: 978 367 385 17 1452 86 12 346
Cuts at: 5615 1972
Size: 1977
Fragments arranged by size:
1977 1452 978 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2196 2217 2373 2469 513
Size: 552 939 192 21 156 96 3664
Fragments arranged by size:
3664 939 552 192 156 96 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2486 487
Size: 683 56 1260 3621
Fragments arranged by size:
3621 1260 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2401 2436 529
Size: 35 1837 35 3713
Fragments arranged by size:
3713 1837 35 35
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2062 4627 809
Size: 507 746 2565 1802
Fragments arranged by size:
2565 1802 746 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2077 2199 2584
Size: 54 1425 50 275 18 247 122 385
Cuts at: 2584 2593 3067 3956 5585 8
Size: 9 474 889 1629 43
Fragments arranged by size:
1629 1425 889 474 385 275 247 122
54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2584 2593 3067 1537
Size: 275 772 9 474 4090
Fragments arranged by size:
4090 772 474 275 9
EarI CTCTTCn'nnn_
Cuts at: 1353 2804 3550 1353
Size: 1451 746 3423
Fragments arranged by size:
3423 1451 746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2201 2697 4207 4581 4727 387
Size: 1064 138 612 496 1510 374 146 1280
Fragments arranged by size:
1510 1280 1064 612 496 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 3683 4280 5067 5335 1239
Size: 393 629 1422 597 787 268 1524
Fragments arranged by size:
1524 1422 787 629 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2633 2874 2633
Size: 241 5379
EcoRI G'AATT_C
Cuts at: 2011 2929 3307 2011
Size: 918 378 4324
Fragments arranged by size:
4324 918 378
EcoRV GAT'ATC
Cuts at: 2539 2539
Size: 5620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 5620
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 2143 4767 1678
Size: 465 2624 2531
Fragments arranged by size:
2624 2531 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2390 2422 2528 2560 4261
Size: 32 135 32 818 32 106 32 1701
Cuts at: 4261 4293 4335 4367 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1701 818 135 106 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2290 4734 4918 1305
Size: 985 2444 184 2007
Fragments arranged by size:
2444 2007 985 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3798
Fragments arranged by size:
3798 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3578 3937 4096 518
Size: 648 2412 359 159 2042
Fragments arranged by size:
2412 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2471 3036 3713 4861 5106 1606
Size: 865 565 677 1148 245 2120
Fragments arranged by size:
2120 1148 865 677 565 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2196 513
Size: 552 939 192 3937
Fragments arranged by size:
3937 939 552 192
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2218 2506 1108
Size: 1110 288 4222
Fragments arranged by size:
4222 1110 288
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PspOMI G'GGCC_C
Cuts at: 2801 2801
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2972 830
Size: 2142 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SacII CC_GC'GG
Cuts at: 2472 2472
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SapI GCTCTTCn'nnn_
Cuts at: 2804 2804
Size: 5620
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2776 2968 4103 4784 4975 826
Size: 219 1731 192 1135 681 191 1471
Fragments arranged by size:
1731 1471 1135 681 219 192 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2076 2592 2076
Size: 516 5104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2258 2579 2947 1872
Size: 386 321 368 4545
Fragments arranged by size:
4545 386 368 321
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3724 4625 4887 820
Size: 197 926 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 2090 2316 1517
Size: 573 226 4821
Fragments arranged by size:
4821 573 226
StyI C'CwwG_G
Cuts at: 513 1065 2004 2196 2289 2529 2634 3059 513
Size: 552 939 192 93 240 105 425 3074
Fragments arranged by size:
3074 939 552 425 240 192 105 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3647 3664 3817 4002 4447 325
Size: 1255 2067 17 153 185 445 1498
Fragments arranged by size:
2067 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2256 2577 2945 1870
Size: 386 321 368 4545
Fragments arranged by size:
4545 386 368 321
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI
SfoI SrfI SwaI XcmI