Restriction map for
pFN21AE0716
(Circular) (Six-base) MAPSORT of: pFN21AE0716.seq Check: 1998 from: 1 to: 5941
pFN21AE0716 5941 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2508 2508
Size: 5941
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2027 2929 4004 5388 5656 1632
Size: 395 902 1075 1384 268 1917
Fragments arranged by size:
1917 1384 1075 902 395 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 2860 4772 2860
Size: 1912 4029
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 2861 1166
Size: 1695 4246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 2196 2977 3203 3684 5256 2196
Size: 781 226 481 1572 2881
Fragments arranged by size:
2881 1572 781 481 226
ApaI G_GGCC'C
Cuts at: 2144 2559 2144
Size: 415 5526
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2453 3250 3476 3628 5737 1493
Size: 960 797 226 152 2109 1697
Fragments arranged by size:
2109 1697 960 797 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2238 2348 2368 2558 2880 3047 3093
Size: 73 295 110 20 190 322 167 46
Cuts at: 3093 3266 1870
Size: 173 4545
Fragments arranged by size:
4545 322 295 190 173 167 110 73
46 20
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2084 2493 2863 3262
Size: 325 217 627 286 11 409 370 399
Cuts at: 3262 4717 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 409 399 370 325 286
217 11
BanII G_rGCy'C
Cuts at: 1391 2144 2367 2559 3048 3093 3260 1391
Size: 753 223 192 489 45 167 4072
Fragments arranged by size:
4072 753 489 223 192 167 45
BbeI G_GCGC'C
Cuts at: 2077 2088 2077
Size: 11 5930
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4970
BbvCI CC'TCA_GC
Cuts at: 2970 2970
Size: 5941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2082 3166 4552
Size: 107 122 71 631 605 410 1084 1386
Cuts at: 4552 136
Size: 1525
Fragments arranged by size:
1525 1386 1084 631 605 410 122 107
71
BglII A'GATC_T
Cuts at: 2703 5936 2703
Size: 3233 2708
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2144 2539 2559 2910 4002 5158 1790
Size: 354 395 20 351 1092 1156 2573
Fragments arranged by size:
2573 1156 1092 395 354 351 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2326 3672 4630
Size: 416 53 83 399 24 909 1346 958
Cuts at: 4630 442
Size: 1753
Fragments arranged by size:
1753 1346 958 909 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2282 2561 2682 3131 4601 1239
Size: 908 135 279 121 449 1470 2579
Fragments arranged by size:
2579 1470 908 449 279 135 121
Bpu10I CC'TnA_GC
Cuts at: 2970 2970
Size: 5941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 2464 3002 4604 882
Size: 617 759 206 538 1602 2219
Fragments arranged by size:
2219 1602 759 617 538 206
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2074 2085 3748 4130
Size: 53 83 186 936 541 11 1663 382
Cuts at: 4130 4765 275
Size: 635 1451
Fragments arranged by size:
1663 1451 936 635 541 382 186 83
53 11
BsaWI w'CCGG_w
Cuts at: 2860 4374 4772 5046 5193 5604 2860
Size: 1514 398 274 147 411 3197
Fragments arranged by size:
3197 1514 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 2882 2912 1178
Size: 30 165 30 861 30 588 30 4207
Fragments arranged by size:
4207 861 588 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2958 3020 3124 1186
Size: 1772 62 104 4003
Fragments arranged by size:
4003 1772 104 62
BseYI C'CCAG_C
Cuts at: 2525 2571 5144 5640 2525
Size: 46 2573 496 2826
Fragments arranged by size:
2826 2573 496 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 2733 1163
Size: 573 606 391 4371
Fragments arranged by size:
4371 606 573 391
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2825 3391 4152 5180 1540
Size: 275 186 824 566 761 1028 2301
Fragments arranged by size:
2301 1028 824 761 566 275 186
BsiHKAI G_wGCw'C
Cuts at: 2367 4002 4087 5158 2367
Size: 1635 85 1071 3150
Fragments arranged by size:
3150 1635 1071 85
BsmI GAATG_Cn'
Cuts at: 2749 3452 3545 2749
Size: 703 93 5145
Fragments arranged by size:
5145 703 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2004 2662 2004
Size: 658 5283
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2367 2539 2559 2910 3048 3093
Size: 399 354 223 172 20 351 138 45
Cuts at: 3093 3260 4002 4087 5158 1391
Size: 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 354 351 223 172
167 138 85 45 20
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2508 3296 844
Size: 1664 788 3489
Fragments arranged by size:
3489 1664 788
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2704 4436 4610 65
Size: 1183 1456 1732 174 1396
Fragments arranged by size:
1732 1456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2216 2391 2860 4585 4772 1700
Size: 110 406 175 469 1725 187 2869
Fragments arranged by size:
2869 1725 469 406 187 175 110
BsrGI T'GTAC_A
Cuts at: 96 2418 96
Size: 2322 3619
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 2682 4745 2682
Size: 2063 3878
BstEII G'GTnAC_C
Cuts at: 1184 2623 1184
Size: 1439 4502
BstYI r'GATC_y
Cuts at: 1972 2703 3271 3638 4023 4040 5492 5578 5590
Size: 731 568 367 385 17 1452 86 12
Cuts at: 5590 5936 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 731 568 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2873 513
Size: 552 939 869 3581
Fragments arranged by size:
3581 939 869 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 2834 3457 4251 4271 2025
Size: 809 623 794 20 3695
Fragments arranged by size:
3695 809 794 623 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2822 2979
Size: 54 1425 50 275 18 378 614 157
Cuts at: 2979 3388 4277 5906 8
Size: 409 889 1629 43
Fragments arranged by size:
1629 1425 889 614 409 378 275 157
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2822 3388 1537
Size: 275 1010 566 4090
Fragments arranged by size:
4090 1010 566 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2715 3871 1353
Size: 1362 1156 3423
Fragments arranged by size:
3423 1362 1156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 2235 4528 4902 5048 387
Size: 1064 138 585 61 2293 374 146 1280
Fragments arranged by size:
2293 1280 1064 585 374 146 138 61
EcoICRI GAG'CTC
Cuts at: 2365 2365
Size: 5941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2147 2282 2561 2682 2929 3131
Size: 393 395 120 135 279 121 247 202
Cuts at: 3131 4004 4601 5388 5656 1239
Size: 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 597 395 393 279 268
247 202 135 121 120
EcoNI CCTnn'n_nnAGG
Cuts at: 2720 2720
Size: 5941
EcoO109I rG'GnC_Cy
Cuts at: 2140 2508 2568 3133 2140
Size: 368 60 565 4948
Fragments arranged by size:
4948 565 368 60
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2077 2088 2278 2608 5088 1678
Size: 399 11 190 330 2480 2531
Fragments arranged by size:
2531 2480 399 330 190 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373
Size: 32 135 32 3010 32 42 32 2626
Fragments arranged by size:
3010 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2073 2084 2073
Size: 11 5930
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 5055 5239 1305
Size: 780 2970 184 2007
Fragments arranged by size:
2970 2007 780 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4119
Fragments arranged by size:
4119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 3899 4258 4417 518
Size: 648 1695 1038 359 159 2042
Fragments arranged by size:
2042 1695 1038 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2303 2575 3125 3357 4034 5182 5427 1606
Size: 697 272 550 232 677 1148 245 2120
Fragments arranged by size:
2120 1148 697 677 550 272 245 232
NaeI GCC'GGC
Cuts at: 2218 2393 2218
Size: 175 5766
NarI GG'CG_CC
Cuts at: 2074 2085 2074
Size: 11 5930
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 2216 2391 2216
Size: 175 5766
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3155 3299 4844 1132
Size: 97 1926 144 1545 2229
Fragments arranged by size:
2229 1926 1545 144 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PfoI T'CCnGG_A
Cuts at: 2259 2259
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2508 2568 3133 2508
Size: 60 565 5316
Fragments arranged by size:
5316 565 60
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2140 2555 2140
Size: 415 5526
PstI C_TGCA'G
Cuts at: 830 2136 2302 3293 830
Size: 1306 166 991 3478
Fragments arranged by size:
3478 1306 991 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2575 3125 2575
Size: 550 5391
SacI G_AGCT'C
Cuts at: 2367 2367
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 2568 3133 2568
Size: 565 5376
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2132 2298 2475 2590 3289 4424 5105
Size: 219 1087 166 177 115 699 1135 681
Cuts at: 5105 5296 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1087 699 681 219 191 177
166 115
SfoI GGC'GCC
Cuts at: 2075 2086 2075
Size: 11 5930
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 2860 1700
Size: 1160 4781
SmaI CCC'GGG
Cuts at: 1872 2240 2350 3095 3268 1872
Size: 368 110 745 173 4545
Fragments arranged by size:
4545 745 368 173 110
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4045 4946 5208 820
Size: 197 926 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2268 2931 3380 513
Size: 552 939 111 153 663 449 3074
Fragments arranged by size:
3074 939 663 552 449 153 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2308 2866 3968 3985 4138 4323 4768
Size: 1255 728 558 1102 17 153 185 445
Cuts at: 4768 325
Size: 1498
Fragments arranged by size:
1498 1255 1102 728 558 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 3220 4187
Size: 274 80 33 51 1443 441 802 967
Cuts at: 4187 5899 96
Size: 1712 138
Fragments arranged by size:
1712 1443 967 802 441 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 2335
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2238 2348 3093 3266 1870
Size: 368 110 745 173 4545
Fragments arranged by size:
4545 745 368 173 110
XmnI GAAnn'nnTTC
Cuts at: 2942 4070 2942
Size: 1128 4813
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SexAI
SfiI SrfI SwaI