Restriction map for
pFN21AE0715
(Circular) (Six-base) MAPSORT of: pFN21AE0715.seq Check: 2700 from: 1 to: 5617
pFN21AE0715 5617 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2583 3427 278
Size: 53 83 186 936 1047 844 2468
Fragments arranged by size:
2468 1047 936 844 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2520 2750 3680 5064 5332 1632
Size: 888 230 930 1384 268 1917
Fragments arranged by size:
1917 1384 930 888 268 230
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 4516 5493 1203
Size: 3313 977 1327
Fragments arranged by size:
3313 1327 977
AgeI A'CCGG_T
Cuts at: 4448 4448
Size: 5617
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 2654 1166
Size: 1488 4129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5617
AlwNI CAG_nnn'CTG
Cuts at: 2651 3360 4932 2651
Size: 709 1572 3336
Fragments arranged by size:
3336 1572 709
ApaLI G'TGCA_C
Cuts at: 3674 4830 3674
Size: 1156 4461
ApoI r'AATT_y
Cuts at: 1493 2926 3152 3304 5413 1493
Size: 1433 226 152 2109 1697
Fragments arranged by size:
2109 1697 1433 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2310 2942 1870
Size: 73 367 632 4545
Fragments arranged by size:
4545 632 367 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2938 4393 618
Size: 325 217 627 1151 1455 1842
Fragments arranged by size:
1842 1455 1151 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 2801 2936 1391
Size: 634 776 135 4072
Fragments arranged by size:
4072 776 634 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2901 928
Size: 971 1002 3644
Fragments arranged by size:
3644 1002 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5612 5612
Size: 5617
BlpI GC'TnA_GC
Cuts at: 3034 3034
Size: 5617
Bme1580I G_kGCm'C
Cuts at: 1790 3678 4834 1790
Size: 1888 1156 2573
Fragments arranged by size:
2573 1888 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4306 442
Size: 416 53 83 399 24 1931 958 1753
Fragments arranged by size:
1931 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2441 4277 1239
Size: 1085 117 1836 2579
Fragments arranged by size:
2579 1836 1085 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 4607 4905 3742
Size: 865 298 4454
Fragments arranged by size:
4454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2580 3424 3806 4441
Size: 53 83 186 936 1047 844 382 635
Cuts at: 4441 275
Size: 1451
Fragments arranged by size:
1451 1047 936 844 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4050 4448 4722 4869 5280 4050
Size: 398 274 147 411 4387
Fragments arranged by size:
4387 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2540 2570 1178
Size: 30 165 30 1137 30 4225
Fragments arranged by size:
4225 1137 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2357 2366 1186
Size: 889 282 9 4437
Fragments arranged by size:
4437 889 282 9
BseYI C'CCAG_C
Cuts at: 4820 5316 4820
Size: 496 5121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5044
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2859 3067 3828 4856 1540
Size: 275 186 858 208 761 1028 2301
Fragments arranged by size:
2301 1028 858 761 275 208 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2366 3678 3763 4834 2025
Size: 341 1312 85 1071 2808
Fragments arranged by size:
2808 1312 1071 341 85
BsmI GAATG_Cn'
Cuts at: 3128 3221 3128
Size: 93 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2366 2801 2936 3678 3763 4834
Size: 399 235 341 435 135 742 85 1071
Cuts at: 4834 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 435 399 341 235 135
85
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 2835 3506 5236 2835
Size: 671 1730 3216
Fragments arranged by size:
3216 1730 671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2972 844
Size: 2128 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2317 2863 3504 1394
Size: 429 494 546 641 3507
Fragments arranged by size:
3507 641 546 494 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4112 4286 65
Size: 1183 2864 174 1396
Fragments arranged by size:
2864 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4261 4448 1700
Size: 110 2451 187 2869
Fragments arranged by size:
2869 2451 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5617
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2947 3314 3699 3716 5168 5254 5266 5612
Size: 975 367 385 17 1452 86 12 346
Cuts at: 5612 1972
Size: 1977
Fragments arranged by size:
1977 1452 975 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2766 513
Size: 552 939 762 3364
Fragments arranged by size:
3364 939 762 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 2016 2649 3133 3927 3947 2016
Size: 633 484 794 20 3686
Fragments arranged by size:
3686 794 633 484 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2856 3064 3953
Size: 54 1425 50 275 18 1026 208 889
Cuts at: 3953 5582 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1026 889 275 208 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2856 3064 1537
Size: 275 1044 208 4090
Fragments arranged by size:
4090 1044 275 208
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2343 2397 3547 1353
Size: 812 178 54 1150 3423
Fragments arranged by size:
3423 1150 812 178 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4204 4578 4724 387
Size: 1064 138 2615 374 146 1280
Fragments arranged by size:
2615 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2023 2023
Size: 5617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2441 2520 2750 3680 4277 5064
Size: 393 692 117 79 230 930 597 787
Cuts at: 5064 5332 1239
Size: 268 1524
Fragments arranged by size:
1524 930 787 692 597 393 268 230
117 79
EcoO109I rG'GnC_Cy
Cuts at: 2107 2259 2107
Size: 152 5465
EcoRI G'AATT_C
Cuts at: 2926 3304 2926
Size: 378 5239
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2312 2041
Size: 271 5346
FspI TGC'GCA
Cuts at: 2843 4123 2843
Size: 1280 4337
FspAI rTGC'GCAy
Cuts at: 2843 2843
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 4764 1678
Size: 3086 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 4258 4290 4332
Size: 32 135 32 968 32 1686 32 42
Cuts at: 4332 4364 1373
Size: 32 2626
Fragments arranged by size:
2626 1686 968 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2961 3207 669
Size: 988 281 1023 246 3079
Fragments arranged by size:
3079 1023 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2294 748
Size: 1546 4071
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2724 4731 4915 1305
Size: 1419 2007 184 2007
Fragments arranged by size:
2007 2007 1419 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2654 3575 3934 4093 518
Size: 648 843 645 921 359 159 2042
Fragments arranged by size:
2042 921 843 648 645 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2153 2419 2684 2872 3033 3710 4858
Size: 466 81 266 265 188 161 677 1148
Cuts at: 4858 5103 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 466 266 265 245 188
161 81
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4126
Fragments arranged by size:
4126 939 552
NdeI CA'TA_TG
Cuts at: 387 2218 387
Size: 1831 3786
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2848 2975 4520 1132
Size: 97 1619 127 1545 2229
Fragments arranged by size:
2229 1619 1545 127 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5617
PciI A'CATG_T
Cuts at: 4516 4516
Size: 5617
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5617
PfoI T'CCnGG_A
Cuts at: 2442 2442
Size: 5617
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2259 2259
Size: 5617
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2206 2969 830
Size: 1376 763 3478
Fragments arranged by size:
3478 1376 763
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
PvuII CAG'CTG
Cuts at: 2153 2419 2872 2153
Size: 266 453 4898
Fragments arranged by size:
4898 453 266
SacI G_AGCT'C
Cuts at: 2025 2025
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SexAI A'CCwGG_T
Cuts at: 2622 2622
Size: 5617
SfcI C'TryA_G
Cuts at: 826 1045 2202 2965 4100 4781 4972 826
Size: 219 1157 763 1135 681 191 1471
Fragments arranged by size:
1471 1157 1135 763 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2312 2944 1872
Size: 440 632 4545
Fragments arranged by size:
4545 632 440
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3721 4622 4884 820
Size: 197 926 1778 901 262 1553
Fragments arranged by size:
1778 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2848 2975 1132
Size: 1716 127 3774
Fragments arranged by size:
3774 1716 127
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 2180 1517
Size: 663 4954
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2181 2506 3056 513
Size: 552 939 12 165 325 550 3074
Fragments arranged by size:
3074 939 552 550 325 165 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2136 2210 3644 3661 3814 3999 4444
Size: 1255 556 74 1434 17 153 185 445
Cuts at: 4444 325
Size: 1498
Fragments arranged by size:
1498 1434 1255 556 445 185 153 74
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5577 5609 5577
Size: 32 5585
Tth111I GACn'n_nGTC
Cuts at: 1316 2225 1316
Size: 909 4708
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2705 2705
Size: 5617
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2310 2942 1870
Size: 440 632 4545
Fragments arranged by size:
4545 632 440
XmnI GAAnn'nnTTC
Cuts at: 3746 3746
Size: 5617
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2581 3425 276
Size: 53 83 186 936 1047 844 2468
Fragments arranged by size:
2468 1047 936 844 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI