Restriction map for
pFN21AE0712
(Circular) (Six-base) MAPSORT of: pFN21AE0712.seq Check: 1901 from: 1 to: 5938
pFN21AE0712 5938 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2501 3748 278
Size: 53 83 186 936 965 1247 2468
Fragments arranged by size:
2468 1247 965 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2616 3219 3281 1937
Size: 679 603 62 4594
Fragments arranged by size:
4594 679 603 62
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2506 4001 5385 5653 1632
Size: 874 1495 1384 268 1917
Fragments arranged by size:
1917 1495 1384 874 268
AfeI AGC'GCT
Cuts at: 2111 2316 2111
Size: 205 5733
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 2350 4837 5814 1203
Size: 1147 2487 977 1327
Fragments arranged by size:
2487 1327 1147 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 2198 1166
Size: 1032 4906
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 3681 5253 3681
Size: 1572 4366
ApaI G_GGCC'C
Cuts at: 2883 2883
Size: 5938
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 3247 3473 3625 5734 1493
Size: 1754 226 152 2109 1697
Fragments arranged by size:
2109 1754 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2170 3263 1870
Size: 73 227 1093 4545
Fragments arranged by size:
4545 1093 227 73
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2636 3211 3259 4714 618
Size: 325 217 627 849 575 48 1455 1842
Fragments arranged by size:
1842 1455 849 627 575 325 217 48
BanII G_rGCy'C
Cuts at: 1391 2177 2184 2883 3257 1391
Size: 786 7 699 374 4072
Fragments arranged by size:
4072 786 699 374 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2347 928
Size: 971 448 4519
Fragments arranged by size:
4519 971 448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3830 5040 3830
Size: 1210 4728
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4549 136
Size: 107 122 71 631 605 2877 1525
Fragments arranged by size:
2877 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2469 3135 5933 2469
Size: 666 2798 2474
Fragments arranged by size:
2798 2474 666
BlpI GC'TnA_GC
Cuts at: 3355 3355
Size: 5938
Bme1580I G_kGCm'C
Cuts at: 1790 2192 2883 3999 5155 1790
Size: 402 691 1116 1156 2573
Fragments arranged by size:
2573 1156 1116 691 402
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2288 2598 3669
Size: 416 53 83 399 24 871 310 1071
Cuts at: 3669 4627 442
Size: 958 1753
Fragments arranged by size:
1753 1071 958 871 416 399 310 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2370 1056
Size: 1314 4624
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2081 2647 2766 2970 4598 1239
Size: 827 15 566 119 204 1628 2579
Fragments arranged by size:
2579 1628 827 566 204 119 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2168 3107 4063 4928 5226 2108
Size: 60 939 956 865 298 2820
Fragments arranged by size:
2820 956 939 865 298 60
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2615 4601 882
Size: 617 1116 1986 2219
Fragments arranged by size:
2219 1986 1116 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 2498 3222 3745
Size: 53 83 186 936 677 288 724 523
Cuts at: 3745 4127 4762 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 724 677 635 523 382 288
186 83 53
BsaWI w'CCGG_w
Cuts at: 2035 4371 4769 5043 5190 5601 2035
Size: 2336 398 274 147 411 2372
Fragments arranged by size:
2372 2336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2689 2719 2832 2862 1178
Size: 30 165 30 1286 30 113 30 4254
Fragments arranged by size:
4254 1286 165 113 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 2771 2840 1186
Size: 1294 291 69 4284
Fragments arranged by size:
4284 1294 291 69
BseYI C'CCAG_C
Cuts at: 2797 3028 5141 5637 2797
Size: 231 2113 496 3098
Fragments arranged by size:
3098 2113 496 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 1163
Size: 573 582 4783
Fragments arranged by size:
4783 582 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3388 4149 5177 1540
Size: 275 186 1012 375 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 375 275 186
BsiHKAI G_wGCw'C
Cuts at: 2184 2462 3999 4084 5155 2184
Size: 278 1537 85 1071 2967
Fragments arranged by size:
2967 1537 1071 278 85
BsmI GAATG_Cn'
Cuts at: 3449 3542 3449
Size: 93 5845
BsmBI CGTCTCn'nnnn_
Cuts at: 2077 2077
Size: 5938
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2184 2192 2462 2883 3257 3999
Size: 399 387 7 8 270 421 374 742
Cuts at: 3999 4084 5155 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 387 374 270
85 8 7
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3293 844
Size: 2449 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2661 2710 3825 1394
Size: 429 838 49 1115 3507
Fragments arranged by size:
3507 1115 838 429 49
BsrDI GCAATG_nn'
Cuts at: 65 1248 2489 4433 4607 65
Size: 1183 1241 1944 174 1396
Fragments arranged by size:
1944 1396 1241 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 4769 1700
Size: 110 2772 187 2869
Fragments arranged by size:
2869 2772 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstYI r'GATC_y
Cuts at: 1972 2469 3135 3268 3635 4020 4037 5489 5575
Size: 497 666 133 367 385 17 1452 86
Cuts at: 5575 5587 5933 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 666 497 385 367 346 133
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2437 487
Size: 683 56 1211 3988
Fragments arranged by size:
3988 1211 683 56
BtsI GCAGTG_nn'
Cuts at: 2222 3196 3454 4248 4268 2222
Size: 974 258 794 20 3892
Fragments arranged by size:
3892 974 794 258 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3385 4274
Size: 54 1425 50 275 18 216 1339 889
Cuts at: 4274 5903 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1339 889 275 216 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2825 3868 1353
Size: 1472 1043 3423
Fragments arranged by size:
3423 1472 1043
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 2501 4525 4899 5045 387
Size: 1064 138 476 436 2024 374 146 1280
Fragments arranged by size:
2024 1280 1064 476 436 374 146 138
EcoICRI GAG'CTC
Cuts at: 2182 2182
Size: 5938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2081 2506 2647 2766 2970 4001
Size: 393 434 15 425 141 119 204 1031
Cuts at: 4001 4598 5385 5653 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1031 787 597 434 425 393 268
204 141 119 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2257 2867 2257
Size: 610 5328
EcoO109I rG'GnC_Cy
Cuts at: 2263 2762 2879 3205 2263
Size: 499 117 326 4996
Fragments arranged by size:
4996 499 326 117
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 2113 2318 3076 5085 1678
Size: 435 205 758 2009 2531
Fragments arranged by size:
2531 2009 758 435 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2762 2794 2832 2864 2943
Size: 32 135 32 1190 32 38 32 79
Cuts at: 2943 2975 4579 4611 4653 4685 1373
Size: 32 1604 32 42 32 2626
Fragments arranged by size:
2626 1604 1190 135 79 42 38 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3220 3282 3528 669
Size: 988 281 1282 62 246 3079
Fragments arranged by size:
3079 1282 988 281 246 62
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5052 5236 1305
Size: 3747 184 2007
Fragments arranged by size:
3747 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4116
Fragments arranged by size:
4116 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 2966 3896 4255 4414 518
Size: 648 1032 768 930 359 159 2042
Fragments arranged by size:
2042 1032 930 768 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3354 4031 5179 5424 1606
Size: 1748 677 1148 245 2120
Fragments arranged by size:
2120 1748 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5386
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 2366 1052
Size: 1314 4624
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2354 3296 4841 1132
Size: 97 1125 942 1545 2229
Fragments arranged by size:
2229 1545 1125 942 97
PasI CC'CwG_GG
Cuts at: 1348 2063 1348
Size: 715 5223
PciI A'CATG_T
Cuts at: 2350 4837 2350
Size: 2487 3451
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2588 1108
Size: 1480 4458
PfoI T'CCnGG_A
Cuts at: 2082 2343 2836 3092 2082
Size: 261 493 256 4928
Fragments arranged by size:
4928 493 261 256
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PspOMI G'GGCC_C
Cuts at: 2879 2879
Size: 5938
PstI C_TGCA'G
Cuts at: 830 2946 3290 830
Size: 2116 344 3478
Fragments arranged by size:
3478 2116 344
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
SacI G_AGCT'C
Cuts at: 2184 2184
Size: 5938
SalI G'TCGA_C
Cuts at: 1936 3218 3280 1936
Size: 1282 62 4594
Fragments arranged by size:
4594 1282 62
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SfcI C'TryA_G
Cuts at: 826 1045 2942 3286 4421 5102 5293 826
Size: 219 1897 344 1135 681 191 1471
Fragments arranged by size:
1897 1471 1135 681 344 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2123 2183 3122 4042 4943 5205
Size: 197 926 180 60 939 920 901 262
Cuts at: 5205 820
Size: 1553
Fragments arranged by size:
1553 939 926 920 901 262 197 180
60
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5938
StyI C'CwwG_G
Cuts at: 513 1065 3377 513
Size: 552 2312 3074
Fragments arranged by size:
3074 2312 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2066 2260 2599 2738 3965 3982
Size: 1255 444 42 194 339 139 1227 17
Cuts at: 3982 4135 4320 4765 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1227 445 444 339 194 185
153 139 42 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2886 4184 5896
Size: 274 80 33 51 1443 909 1298 1712
Cuts at: 5896 96
Size: 138
Fragments arranged by size:
1712 1443 1298 909 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2689 2721 5898 5930 2689
Size: 32 3177 32 2697
Fragments arranged by size:
3177 2697 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2612 1316
Size: 1296 4642
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 2159 4067 2159
Size: 1908 4030
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2499 3746 276
Size: 53 83 186 936 965 1247 2468
Fragments arranged by size:
2468 1247 965 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI