Restriction map for
pFN21AE0709
(Circular) (Six-base) MAPSORT of: pFN21AE0709.seq Check: 9086 from: 1 to: 5590
pFN21AE0709 5590 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2220 2933 1937
Size: 283 713 4594
Fragments arranged by size:
4594 713 283
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2520 3653 5037 5305 1632
Size: 695 193 1133 1384 268 1917
Fragments arranged by size:
1917 1384 1133 695 268 193
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 2385 4489 5466 1203
Size: 1182 2104 977 1327
Fragments arranged by size:
2104 1327 1182 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AlwNI CAG_nnn'CTG
Cuts at: 3333 4905 3333
Size: 1572 4018
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2245 2270 2791 2899 3125 3277 5386 1493
Size: 752 25 521 108 226 152 2109 1697
Fragments arranged by size:
2109 1697 752 521 226 152 108 25
AseI AT'TA_AT
Cuts at: 160 1032 2102 4144 160
Size: 872 1070 2042 1606
Fragments arranged by size:
2042 1606 1070 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2784 2915 1870
Size: 73 841 131 4545
Fragments arranged by size:
4545 841 131 73
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2911 4366 618
Size: 325 217 627 1124 1455 1842
Fragments arranged by size:
1842 1455 1124 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2909 1391
Size: 1518 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 928
Size: 971 416 4203
Fragments arranged by size:
4203 971 416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2111 3482 4692 2111
Size: 1371 1210 3009
Fragments arranged by size:
3009 1371 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4201 136
Size: 107 122 71 631 605 337 2192 1525
Fragments arranged by size:
2192 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 3651 4807 1790
Size: 1861 1156 2573
Fragments arranged by size:
2573 1861 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5590
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2835 3321 4279
Size: 416 53 83 399 24 1418 486 958
Cuts at: 4279 442
Size: 1753
Fragments arranged by size:
1753 1418 958 486 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2561 4250 1239
Size: 1322 1689 2579
Fragments arranged by size:
2579 1689 1322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3715 4580 4878 3715
Size: 865 298 4427
Fragments arranged by size:
4427 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4253 882
Size: 617 2754 2219
Fragments arranged by size:
2754 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3381 493
Size: 711 2177 2702
Fragments arranged by size:
2702 2177 711
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3397 3779 4414 275
Size: 53 83 186 936 1864 382 635 1451
Fragments arranged by size:
1864 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5365
Fragments arranged by size:
5365 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5590
BseYI C'CCAG_C
Cuts at: 4793 5289 4793
Size: 496 5094
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5017
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 3801 4829 1540
Size: 275 186 1039 761 1028 2301
Fragments arranged by size:
2301 1039 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3651 3736 4807 3651
Size: 85 1071 4434
Fragments arranged by size:
4434 1071 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2909 3651 3736 4807 1391
Size: 399 1119 742 85 1071 2174
Fragments arranged by size:
2174 1119 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2945 844
Size: 2101 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3477 1394
Size: 429 1654 3507
Fragments arranged by size:
3507 1654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2883 4085 4259 65
Size: 1183 1635 1202 174 1396
Fragments arranged by size:
1635 1396 1202 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4234 4421 1700
Size: 110 2424 187 2869
Fragments arranged by size:
2869 2424 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5590
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2043 2920 3287 3672 3689 5141 5227 5239
Size: 71 877 367 385 17 1452 86 12
Cuts at: 5239 5585 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 877 385 367 346 86 71
17 12
Bsu36I CC'TnA_GG
Cuts at: 2609 2609
Size: 5590
BtgI C'CryG_G
Cuts at: 513 1065 2475 513
Size: 552 1410 3628
Fragments arranged by size:
3628 1410 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 3106 3900 3920 3106
Size: 794 20 4776
Fragments arranged by size:
4776 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3037 3926 5555
Size: 54 1425 50 275 18 1207 889 1629
Cuts at: 5555 8
Size: 43
Fragments arranged by size:
1629 1425 1207 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3520 1353
Size: 2167 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2520 2561 3653 4250 5037 5305
Size: 393 695 193 41 1092 597 787 268
Cuts at: 5305 1239
Size: 1524
Fragments arranged by size:
1524 1092 787 695 597 393 268 193
41
EcoRI G'AATT_C
Cuts at: 2245 2899 3277 2245
Size: 654 378 4558
Fragments arranged by size:
4558 654 378
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 4737 1678
Size: 3059 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2056 2088 4231 4263 4305
Size: 32 135 32 484 32 2143 32 42
Cuts at: 4305 4337 1373
Size: 32 2626
Fragments arranged by size:
2626 2143 484 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2203 2934 3180 669
Size: 988 281 265 731 246 3079
Fragments arranged by size:
3079 988 731 281 265 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3768
Fragments arranged by size:
3768 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2806 3548 3907 4066 518
Size: 648 1640 742 359 159 2042
Fragments arranged by size:
2042 1640 742 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3006 3683 4831 5076 1606
Size: 1400 677 1148 245 2120
Fragments arranged by size:
2120 1400 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5038
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2232 2538 2948 4493 1132
Size: 97 1003 306 410 1545 2229
Fragments arranged by size:
2229 1545 1003 410 306 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5590
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5590
PfoI T'CCnGG_A
Cuts at: 2049 2552 2049
Size: 503 5087
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PspXI vC'TCGA_Gb
Cuts at: 2784 2784
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2942 830
Size: 2112 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2938 4073 4754 4945 826
Size: 219 1893 1135 681 191 1471
Fragments arranged by size:
1893 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2784 3694 4595 4857 820
Size: 197 926 841 910 901 262 1553
Fragments arranged by size:
1553 926 910 901 841 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 2614 1517
Size: 1097 4493
StyI C'CwwG_G
Cuts at: 513 1065 3029 513
Size: 552 1964 3074
Fragments arranged by size:
3074 1964 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3617 3634 3787 3972 4417 325
Size: 1255 2037 17 153 185 445 1498
Fragments arranged by size:
2037 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 2299 2515
Size: 274 80 33 51 1443 105 217 216
Cuts at: 2515 3836 5548 96
Size: 1321 1712 138
Fragments arranged by size:
1712 1443 1321 274 217 216 138 105
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5550 5582 5550
Size: 32 5558
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 2784 1943
Size: 841 4749
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI