Restriction map for
pFN21AE0708
(Circular) (Six-base) MAPSORT of: pFN21AE0708.seq Check: 8065 from: 1 to: 5926
pFN21AE0708 5926 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2243 2243
Size: 5926
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2500 2752 3989 5373 5641 1632
Size: 868 252 1237 1384 268 1917
Fragments arranged by size:
1917 1384 1237 868 268 252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 2810 4825 5802 1203
Size: 1607 2015 977 1327
Fragments arranged by size:
2015 1607 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 2280 2364 1166
Size: 1114 84 4728
Fragments arranged by size:
4728 1114 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 2356 3669 5241 2356
Size: 1313 1572 3041
Fragments arranged by size:
3041 1572 1313
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 2069 2868 2912 3167 3235 3461 3613 5722
Size: 576 799 44 255 68 226 152 2109
Cuts at: 5722 1493
Size: 1697
Fragments arranged by size:
2109 1697 799 576 255 226 152 68
44
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 3251 1870
Size: 73 1308 4545
Fragments arranged by size:
4545 1308 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2194 3247 4702 618
Size: 325 217 627 407 1053 1455 1842
Fragments arranged by size:
1842 1455 1053 627 407 325 217
BanII G_rGCy'C
Cuts at: 1391 3245 1391
Size: 1854 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2506 928
Size: 971 162 445 4348
Fragments arranged by size:
4348 971 445 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4117 4151 1903
Size: 34 2180 34 3678
Fragments arranged by size:
3678 2180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BfrBI ATG'CAT
Cuts at: 2655 2655
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4537 136
Size: 107 122 71 631 605 337 2528 1525
Fragments arranged by size:
2528 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 3343 3343
Size: 5926
Bme1580I G_kGCm'C
Cuts at: 1790 2042 3987 5143 1790
Size: 252 1945 1156 2573
Fragments arranged by size:
2573 1945 1156 252
BmgBI CAC'GTC
Cuts at: 1318 2059 1318
Size: 741 5185
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2589 3657 4615
Size: 416 53 83 399 24 1172 1068 958
Cuts at: 4615 442
Size: 1753
Fragments arranged by size:
1753 1172 1068 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2543 2898 2982 3082 3150 4586 1239
Size: 917 387 355 84 100 68 1436 2579
Fragments arranged by size:
2579 1436 917 387 355 100 84 68
Bpu10I CC'TnA_GC
Cuts at: 2021 2543 2021
Size: 522 5404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4589 882
Size: 617 3090 2219
Fragments arranged by size:
3090 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2811 3717 493
Size: 711 1607 906 2702
Fragments arranged by size:
2702 1607 906 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3733 4115 4750 275
Size: 53 83 186 936 2200 382 635 1451
Fragments arranged by size:
2200 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2453 2483 1178
Size: 30 165 30 1050 30 4621
Fragments arranged by size:
4621 1050 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2474 1186
Size: 1288 4638
BseYI C'CCAG_C
Cuts at: 5129 5625 5129
Size: 496 5430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 3085 3169 1163
Size: 573 606 743 84 3920
Fragments arranged by size:
3920 743 606 573 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 3376 4137 5165 1540
Size: 275 186 15 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2427 3987 4072 5143 2427
Size: 1560 85 1071 3210
Fragments arranged by size:
3210 1560 1071 85
BsmI GAATG_Cn'
Cuts at: 2984 3437 3530 2984
Size: 453 93 5380
Fragments arranged by size:
5380 453 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2427 3245 3987 4072 5143 1391
Size: 399 252 385 818 742 85 1071 2174
Fragments arranged by size:
2174 1071 818 742 399 385 252 85
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2243 3281 844
Size: 1399 1038 3489
Fragments arranged by size:
3489 1399 1038
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2937 4421 4595 65
Size: 1183 1689 1484 174 1396
Fragments arranged by size:
1689 1484 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4570 4757 1700
Size: 110 2760 187 2869
Fragments arranged by size:
2869 2760 187 110
BsrGI T'GTAC_A
Cuts at: 96 2857 96
Size: 2761 3165
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 2693 3986 4998 2693
Size: 1293 1012 3621
Fragments arranged by size:
3621 1293 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2356 794
Size: 1562 4364
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 3256 3623 4008 4025 5477 5563 5575 5921
Size: 1284 367 385 17 1452 86 12 346
Cuts at: 5921 1972
Size: 1977
Fragments arranged by size:
1977 1452 1284 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4435
Fragments arranged by size:
4435 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 2989 3073 3157 3442 4236 4256 2989
Size: 84 84 285 794 20 4659
Fragments arranged by size:
4659 794 285 84 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 3373 4262
Size: 54 1425 50 275 18 183 1360 889
Cuts at: 4262 5891 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1360 889 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 3373 1537
Size: 275 201 1360 4090
Fragments arranged by size:
4090 1360 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2471 3187 3856 1353
Size: 1118 716 669 3423
Fragments arranged by size:
3423 1118 716 669
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2500 2543 2752 2898 2982 3082
Size: 393 524 344 43 209 146 84 100
Cuts at: 3082 3150 3989 4586 5373 5641 1239
Size: 68 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 597 524 393 344 268
209 146 100 84 68 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2250 2099
Size: 151 5775
EcoRI G'AATT_C
Cuts at: 2868 3235 3613 2868
Size: 367 378 5181
Fragments arranged by size:
5181 378 367
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 5073 1678
Size: 3395 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2594 2626 4567 4599 4641
Size: 32 135 32 1022 32 1941 32 42
Cuts at: 4641 4673 1373
Size: 32 2626
Fragments arranged by size:
2626 1941 1022 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5040 5224 1305
Size: 3735 184 2007
Fragments arranged by size:
3735 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4104
Fragments arranged by size:
4104 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2280 2364 2418 2893 2977 3884 4243
Size: 648 1114 84 54 475 84 907 359
Cuts at: 4243 4402 518
Size: 159 2042
Fragments arranged by size:
2042 1114 907 648 475 359 159 84
84 54
MspA1I CmG'CkG
Cuts at: 1606 2643 3342 4019 5167 5412 1606
Size: 1037 699 677 1148 245 2120
Fragments arranged by size:
2120 1148 1037 699 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4435
Fragments arranged by size:
4435 939 552
NdeI CA'TA_TG
Cuts at: 387 2890 2974 387
Size: 2503 84 3339
Fragments arranged by size:
3339 2503 84
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 2013 3373 2013
Size: 1360 4566
NsiI A_TGCA'T
Cuts at: 2657 2657
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2321 2495 3284 4829 1132
Size: 97 1092 174 789 1545 2229
Fragments arranged by size:
2229 1545 1092 789 174 97
PasI CC'CwG_GG
Cuts at: 1348 2161 1348
Size: 813 5113
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2234 1613
Size: 621 5305
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PstI C_TGCA'G
Cuts at: 830 3278 830
Size: 2448 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2643 2643
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 2443 2709 3274 4409 5090 5281 826
Size: 219 1398 266 565 1135 681 191 1471
Fragments arranged by size:
1471 1398 1135 681 565 266 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 3253 1872
Size: 1381 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 2372 1517
Size: 855 5071
StyI C'CwwG_G
Cuts at: 513 1065 2004 3365 513
Size: 552 939 1361 3074
Fragments arranged by size:
3074 1361 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3953 3970 4123 4308 4753 325
Size: 1255 2373 17 153 185 445 1498
Fragments arranged by size:
2373 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2857 4172 5884
Size: 274 80 33 51 1443 880 1315 1712
Cuts at: 5884 96
Size: 138
Fragments arranged by size:
1712 1443 1315 880 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2642 2642
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 3251 1870
Size: 1381 4545
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI