Restriction map for
pFN21AE0706
(Circular) (Six-base) MAPSORT of: pFN21AE0706.seq Check: 5480 from: 1 to: 5926
pFN21AE0706 5926 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3989 5373 5641 1632
Size: 2357 1384 268 1917
Fragments arranged by size:
2357 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 3223 4825 5802 1203
Size: 2020 1602 977 1327
Fragments arranged by size:
2020 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 2373 1166
Size: 1207 4719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 3669 5241 3669
Size: 1572 4354
ApaI G_GGCC'C
Cuts at: 3155 3155
Size: 5926
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 2174 2361 3235 3461 3613 5722 1493
Size: 681 187 874 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 681 226 187 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2154 2265 2463 2574 2951 3251
Size: 73 71 140 111 198 111 377 300
Cuts at: 3251 1870
Size: 4545
Fragments arranged by size:
4545 377 300 198 140 111 111 73
71
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2609 2713 2858 3247
Size: 325 217 627 231 591 104 145 389
Cuts at: 3247 4702 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 591 389 325 231 217
145 104
BanII G_rGCy'C
Cuts at: 1391 2391 2770 3155 3245 1391
Size: 1000 379 385 90 4072
Fragments arranged by size:
4072 1000 385 379 90
BbeI G_GCGC'C
Cuts at: 2022 2022
Size: 5926
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4117 4151 1903
Size: 34 2180 34 3678
Fragments arranged by size:
3678 2180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BfrBI ATG'CAT
Cuts at: 2524 2524
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2680 4537 136
Size: 107 122 71 631 605 1008 1857 1525
Fragments arranged by size:
1857 1525 1008 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 2568 3024 3343 2568
Size: 456 319 5151
Fragments arranged by size:
5151 456 319
Bme1580I G_kGCm'C
Cuts at: 1790 2640 2863 3155 3987 5143 1790
Size: 850 223 292 832 1156 2573
Fragments arranged by size:
2573 1156 850 832 292 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3657 4615 442
Size: 416 53 83 399 24 2240 958 1753
Fragments arranged by size:
2240 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 2838 4586 1239
Size: 841 758 1748 2579
Fragments arranged by size:
2579 1748 841 758
Bpu10I CC'TnA_GC
Cuts at: 2390 2390
Size: 5926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3091 4051 4916 5214 3091
Size: 960 865 298 3803
Fragments arranged by size:
3803 960 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4589 882
Size: 617 3090 2219
Fragments arranged by size:
3090 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2679 3717 493
Size: 711 1475 1038 2702
Fragments arranged by size:
2702 1475 1038 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 2445 3733 4115
Size: 53 83 186 936 486 426 1288 382
Cuts at: 4115 4750 275
Size: 635 1451
Fragments arranged by size:
1451 1288 936 635 486 426 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2097 2127 1178
Size: 30 165 30 694 30 4977
Fragments arranged by size:
4977 694 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2778 2925 1186
Size: 1592 147 4187
Fragments arranged by size:
4187 1592 147
BseYI C'CCAG_C
Cuts at: 2655 3101 5129 5625 2655
Size: 446 2028 496 2956
Fragments arranged by size:
2956 2028 496 446
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2294 3376 4137 5165 1540
Size: 275 186 293 1082 761 1028 2301
Fragments arranged by size:
2301 1082 1028 761 293 275 186
BsiHKAI G_wGCw'C
Cuts at: 2066 2391 3987 4072 5143 2066
Size: 325 1596 85 1071 2849
Fragments arranged by size:
2849 1596 1071 325 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
BsmBI CGTCTCn'nnnn_
Cuts at: 2991 2991
Size: 5926
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2391 2640 2770 2863 3155 3245
Size: 399 276 325 249 130 93 292 90
Cuts at: 3245 3987 4072 5143 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 325 292 276 249
130 93 90 85
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 2127 2433 3815 5545 2127
Size: 306 1382 1730 2508
Fragments arranged by size:
2508 1730 1382 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 3281 844
Size: 2200 237 3489
Fragments arranged by size:
3489 2200 237
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2716 3161 4570 4757 1700
Size: 110 906 445 1409 187 2869
Fragments arranged by size:
2869 1409 906 445 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5926
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 3256 3623 4008 4025 5477 5563 5575 5921
Size: 1284 367 385 17 1452 86 12 346
Cuts at: 5921 1972
Size: 1977
Fragments arranged by size:
1977 1452 1284 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2847 2847
Size: 5926
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5374
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 3442 4236 4256 3442
Size: 794 20 5112
Fragments arranged by size:
5112 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2175 2210 2690 2725 529
Size: 35 1611 35 480 35 3730
Fragments arranged by size:
3730 1611 480 35 35 35
DraI TTT'AAA
Cuts at: 2179 3230 2179
Size: 1051 4875
DraIII CAC_nnn'GTG
Cuts at: 3199 3199
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2513 4933 809
Size: 507 1197 2420 1802
Fragments arranged by size:
2420 1802 1197 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2233 2291
Size: 54 1425 50 275 18 378 25 58
Cuts at: 2291 3373 4262 5891 8
Size: 1082 889 1629 43
Fragments arranged by size:
1629 1425 1082 889 378 275 58 54
50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2291 3373 1537
Size: 275 479 1082 4090
Fragments arranged by size:
4090 1082 479 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 2755 3856 1353
Size: 692 710 1101 3423
Fragments arranged by size:
3423 1101 710 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2389 2389
Size: 5926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2838 3989 4586 5373 5641 1239
Size: 393 448 758 1151 597 787 268 1524
Fragments arranged by size:
1524 1151 787 758 597 448 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2817 2817
Size: 5926
EcoO109I rG'GnC_Cy
Cuts at: 2315 2460 3128 3151 3152 2315
Size: 145 668 23 1 5089
Fragments arranged by size:
5089 668 145 23 1
EcoRI G'AATT_C
Cuts at: 2361 3235 3613 2361
Size: 874 378 4674
Fragments arranged by size:
4674 874 378
FspI TGC'GCA
Cuts at: 2084 4432 2084
Size: 2348 3578
HaeII r_GCGC'y
Cuts at: 1678 2022 2597 5073 1678
Size: 344 575 2476 2531
Fragments arranged by size:
2531 2476 575 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 4567 4599 4641
Size: 32 135 32 911 32 2052 32 42
Cuts at: 4641 4673 1373
Size: 32 2626
Fragments arranged by size:
2626 2052 911 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2518 3270 3516 669
Size: 988 281 580 752 246 3079
Fragments arranged by size:
3079 988 752 580 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 2018 2018
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 2371 3525 2371
Size: 1154 4772
MluI A'CGCG_T
Cuts at: 3223 5802 3223
Size: 2579 3347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5040 5224 1305
Size: 3735 184 2007
Fragments arranged by size:
3735 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 10
Size: 54 1425 343 378 3726
Fragments arranged by size:
3726 1425 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2373 3884 4243 4402 518
Size: 648 1207 1511 359 159 2042
Fragments arranged by size:
2042 1511 1207 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3171 3342 4019 5167 5412 1606
Size: 1565 171 677 1148 245 2120
Fragments arranged by size:
2120 1565 1148 677 245 171
NaeI GCC'GGC
Cuts at: 2718 3163 2718
Size: 445 5481
NarI GG'CG_CC
Cuts at: 2019 2019
Size: 5926
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5374
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NgoMIV G'CCGG_C
Cuts at: 2716 3161 2716
Size: 445 5481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NsiI A_TGCA'T
Cuts at: 2526 2526
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2524 3284 4829 1132
Size: 97 1295 760 1545 2229
Fragments arranged by size:
2229 1545 1295 760 97
PasI CC'CwG_GG
Cuts at: 1348 2836 1348
Size: 1488 4438
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2619 2655 1108
Size: 1511 36 4379
Fragments arranged by size:
4379 1511 36
PfoI T'CCnGG_A
Cuts at: 2815 2815
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PmlI CAC'GTG
Cuts at: 2679 2679
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2315 3128 2315
Size: 813 5113
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PspOMI G'GGCC_C
Cuts at: 3151 3151
Size: 5926
PstI C_TGCA'G
Cuts at: 830 2758 3278 830
Size: 1928 520 3478
Fragments arranged by size:
3478 1928 520
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
SacI G_AGCT'C
Cuts at: 2391 2391
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SanDI GG'GwC_CC
Cuts at: 3128 3128
Size: 5926
SapI GCTCTTCn'nnn_
Cuts at: 2045 2045
Size: 5926
SbfI CC_TGCA'GG
Cuts at: 2758 3278 2758
Size: 520 5406
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 2754 3274 4409 5090 5281 826
Size: 219 1709 520 1135 681 191 1471
Fragments arranged by size:
1709 1471 1135 681 520 219 191
SfoI GGC'GCC
Cuts at: 2020 2020
Size: 5926
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 3161 1700
Size: 1461 4465
SmaI CCC'GGG
Cuts at: 1872 2267 2953 3253 1872
Size: 395 686 300 4545
Fragments arranged by size:
4545 686 395 300
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2154 3070 4030 4931 5193 820
Size: 197 926 211 916 960 901 262 1553
Fragments arranged by size:
1553 960 926 916 901 262 211 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 3365 513
Size: 552 2300 3074
Fragments arranged by size:
3074 2300 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2701 3953 3970 4123 4308 4753 325
Size: 1255 1121 1252 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1252 1121 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4172 5884 96
Size: 274 80 33 51 1443 2195 1712 138
Fragments arranged by size:
2195 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2697 2729 5886 5918 2697
Size: 32 3157 32 2705
Fragments arranged by size:
3157 2705 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 2154 1943
Size: 211 5715
XmaI C'CCGG_G
Cuts at: 1870 2265 2951 3251 1870
Size: 395 686 300 4545
Fragments arranged by size:
4545 686 395 300
XmnI GAAnn'nnTTC
Cuts at: 2039 4055 2039
Size: 2016 3910
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NruI
PacI PspXI PsrI PvuII RsrII SacII SexAI SfiI
SrfI SwaI XcmI