Restriction map for
pFN21AE0705
(Circular) (Six-base) MAPSORT of: pFN21AE0705.seq Check: 2865 from: 1 to: 5590
pFN21AE0705 5590 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 2933 1937
Size: 72 924 4594
Fragments arranged by size:
4594 924 72
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 2694 3653 5037 5305 1632
Size: 716 346 959 1384 268 1917
Fragments arranged by size:
1917 1384 959 716 346 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 2584 1166
Size: 1418 4172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 1184
Size: 948 4642
AlwNI CAG_nnn'CTG
Cuts at: 2672 3333 4905 2672
Size: 661 1572 3357
Fragments arranged by size:
3357 1572 661
ApaI G_GGCC'C
Cuts at: 2339 2339
Size: 5590
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2308 2356 2521 2899 3125 3277 5386 1493
Size: 815 48 165 378 226 152 2109 1697
Fragments arranged by size:
2109 1697 815 378 226 165 152 48
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2915 1870
Size: 73 972 4545
Fragments arranged by size:
4545 972 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2754 2787 2754
Size: 33 5557
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2911 4366 618
Size: 325 217 627 1124 1455 1842
Fragments arranged by size:
1842 1455 1124 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2339 2909 1391
Size: 948 570 4072
Fragments arranged by size:
4072 948 570
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4201 136
Size: 107 122 71 631 605 2529 1525
Fragments arranged by size:
2529 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 2339 3651 4807 1790
Size: 549 1312 1156 2573
Fragments arranged by size:
2573 1312 1156 549
BmgBI CAC'GTC
Cuts at: 1318 2275 2384 1318
Size: 957 109 4524
Fragments arranged by size:
4524 957 109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 3321 4279
Size: 416 53 83 399 24 639 1265 958
Cuts at: 4279 442
Size: 1753
Fragments arranged by size:
1753 1265 958 639 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2029 4250 1239
Size: 790 2221 2579
Fragments arranged by size:
2579 2221 790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2805 3715 4580 4878 2805
Size: 910 865 298 3517
Fragments arranged by size:
3517 910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4253 882
Size: 617 2754 2219
Fragments arranged by size:
2754 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3381 493
Size: 711 2177 2702
Fragments arranged by size:
2702 2177 711
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3397 3779 4414 275
Size: 53 83 186 936 1864 382 635 1451
Fragments arranged by size:
1864 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2661 2691 1178
Size: 30 165 30 1258 30 4077
Fragments arranged by size:
4077 1258 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2397 1186
Size: 1211 4379
BseYI C'CCAG_C
Cuts at: 2332 4793 5289 2332
Size: 2461 496 2633
Fragments arranged by size:
2633 2461 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 2396 1163
Size: 573 567 93 4357
Fragments arranged by size:
4357 573 567 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 3801 4829 1540
Size: 275 186 1039 761 1028 2301
Fragments arranged by size:
2301 1039 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3651 3736 4807 3651
Size: 85 1071 4434
Fragments arranged by size:
4434 1071 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2339 2909 3651 3736 4807 1391
Size: 399 549 570 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 549 399 85
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 2701 3479 5209 2701
Size: 778 1730 3082
Fragments arranged by size:
3082 1730 778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2945 844
Size: 2101 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3477 1394
Size: 429 1654 3507
Fragments arranged by size:
3507 1654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2880 4085 4259 65
Size: 1183 1632 1205 174 1396
Fragments arranged by size:
1632 1396 1205 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4234 4421 1700
Size: 110 2424 187 2869
Fragments arranged by size:
2869 2424 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5590
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstXI CCAn_nnnn'nTGG
Cuts at: 2586 2586
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585
Size: 948 367 385 17 1452 86 12 346
Cuts at: 5585 1972
Size: 1977
Fragments arranged by size:
1977 1452 948 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2314 513
Size: 552 1249 3789
Fragments arranged by size:
3789 1249 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 3106 3900 3920 3106
Size: 794 20 4776
Fragments arranged by size:
4776 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2370 2405 529
Size: 35 1806 35 3714
Fragments arranged by size:
3714 1806 35 35
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3037 3926 5555
Size: 54 1425 50 275 18 1207 889 1629
Cuts at: 5555 8
Size: 43
Fragments arranged by size:
1629 1425 1207 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3520 1353
Size: 2167 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2348 2694 3653 4250 5037 5305
Size: 393 397 319 346 959 597 787 268
Cuts at: 5305 1239
Size: 1524
Fragments arranged by size:
1524 959 787 597 397 393 346 319
268
EcoO109I rG'GnC_Cy
Cuts at: 2336 2336
Size: 5590
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
EcoRV GAT'ATC
Cuts at: 2549 2549
Size: 5590
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 4737 1678
Size: 3059 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373
Size: 32 135 32 2659 32 42 32 2626
Fragments arranged by size:
2659 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2641 2934 3180 669
Size: 988 281 703 293 246 3079
Fragments arranged by size:
3079 988 703 293 281 246
HindIII A'AGCT_T
Cuts at: 748 2176 748
Size: 1428 4162
HpaI GTT'AAC
Cuts at: 2641 3180 2641
Size: 539 5051
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2116 4704 4888 1305
Size: 811 2588 184 2007
Fragments arranged by size:
2588 2007 811 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3768
Fragments arranged by size:
3768 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2584 2652 3548 3907 4066 518
Size: 648 1418 68 896 359 159 2042
Fragments arranged by size:
2042 1418 896 648 359 159 68
MspA1I CmG'CkG
Cuts at: 1606 2075 2636 3006 3683 4831 5076 1606
Size: 469 561 370 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 561 469 370 245
NcoI C'CATG_G
Cuts at: 513 1065 2314 513
Size: 552 1249 3789
Fragments arranged by size:
3789 1249 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2948 4493 1132
Size: 97 1719 1545 2229
Fragments arranged by size:
2229 1719 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5590
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2720 1108
Size: 1612 3978
PfoI T'CCnGG_A
Cuts at: 2665 2665
Size: 5590
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PspOMI G'GGCC_C
Cuts at: 2335 2335
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2942 830
Size: 2112 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
PvuII CAG'CTG
Cuts at: 2075 2636 2075
Size: 561 5029
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2938 4073 4754 4945 826
Size: 219 1893 1135 681 191 1471
Fragments arranged by size:
1893 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2820 3694 4595 4857 820
Size: 197 926 877 874 901 262 1553
Fragments arranged by size:
1553 926 901 877 874 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2086 2314 3029 513
Size: 552 1021 228 715 3074
Fragments arranged by size:
3074 1021 715 552 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 2766 3617 3634 3787 3972 4417
Size: 1255 495 691 851 17 153 185 445
Cuts at: 4417 325
Size: 1498
Fragments arranged by size:
1498 1255 851 691 495 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3836 5548 96
Size: 274 80 33 51 1443 1859 1712 138
Fragments arranged by size:
1859 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2464 2496 5550 5582 2464
Size: 32 3054 32 2472
Fragments arranged by size:
3054 2472 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI